全文获取类型
收费全文 | 106690篇 |
免费 | 10448篇 |
国内免费 | 6108篇 |
专业分类
耳鼻咽喉 | 806篇 |
儿科学 | 980篇 |
妇产科学 | 1399篇 |
基础医学 | 29589篇 |
口腔科学 | 3225篇 |
临床医学 | 6918篇 |
内科学 | 14700篇 |
皮肤病学 | 2023篇 |
神经病学 | 8447篇 |
特种医学 | 2137篇 |
外国民族医学 | 23篇 |
外科学 | 8600篇 |
综合类 | 15691篇 |
现状与发展 | 21篇 |
一般理论 | 2篇 |
预防医学 | 2995篇 |
眼科学 | 2846篇 |
药学 | 10763篇 |
19篇 | |
中国医学 | 3195篇 |
肿瘤学 | 8867篇 |
出版年
2024年 | 166篇 |
2023年 | 1205篇 |
2022年 | 2278篇 |
2021年 | 3252篇 |
2020年 | 3140篇 |
2019年 | 2763篇 |
2018年 | 2893篇 |
2017年 | 3414篇 |
2016年 | 3903篇 |
2015年 | 4407篇 |
2014年 | 6713篇 |
2013年 | 8356篇 |
2012年 | 6427篇 |
2011年 | 7478篇 |
2010年 | 6052篇 |
2009年 | 5811篇 |
2008年 | 6120篇 |
2007年 | 6077篇 |
2006年 | 5589篇 |
2005年 | 4803篇 |
2004年 | 4057篇 |
2003年 | 3362篇 |
2002年 | 2535篇 |
2001年 | 2150篇 |
2000年 | 1877篇 |
1999年 | 1619篇 |
1998年 | 1508篇 |
1997年 | 1417篇 |
1996年 | 1301篇 |
1995年 | 1352篇 |
1994年 | 1197篇 |
1993年 | 1054篇 |
1992年 | 864篇 |
1991年 | 827篇 |
1990年 | 702篇 |
1989年 | 712篇 |
1988年 | 586篇 |
1987年 | 542篇 |
1986年 | 472篇 |
1985年 | 710篇 |
1984年 | 638篇 |
1983年 | 438篇 |
1982年 | 552篇 |
1981年 | 423篇 |
1980年 | 352篇 |
1979年 | 313篇 |
1978年 | 219篇 |
1977年 | 169篇 |
1976年 | 154篇 |
1975年 | 64篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
钙拮抗剂TMB-8抑制BHQ,NE和KCl引起的培养乳牛基底动脉单个平滑肌细胞内游离钙的升高 总被引:1,自引:0,他引:1
用AR CM MIC阳离子测定系统,测量单个细胞内游离钙浓度([Ca2+]i),研究8-(N,N-二乙胺)-n-辛基 3,4,5-三甲氧基苯甲酸酯(TMB-8)对培养乳牛基底动脉平滑肌[Ca2+]i的作用。在细胞外钙浓度为1.3mmol·L-1时,TMB-8(30μmol·L-1)可明显抑制BHQ,NE及KCl引起[Ca2+]i的升高。在细胞外钙为零+EGTA 0.1mmol·L-1时,TMB-8(10,30及100μmol·L-1)可浓度依赖性地降低静息[Ca2+]i,TMB-8(30μmol·L-1)可几乎完全阻断BHQ及NE引起[Ca2+]i的增加。研究表明TMB-8降低培养乳牛基底动脉平滑肌[Ca2+]i的机制,主要是抑制肌浆网Ca2+的释放,或增加肌浆网对Ca2+的摄入,并由此间接地抑制细胞外钙的内流。 相似文献
72.
M. E. Perry Y. Mustafa S. K. Wood M. I. D. Cawley D. C. Dumonde K. A. Brown 《Rheumatology international》1997,17(4):169-174
A morphological examination of synovial tissue from 25 patients with rheumatoid arthritis revealed that binucleated or multinucleated
plasma cells were present in all samples and absent in synovia obtained from 16 control patients. Plasma cells containing
two, three of four nuclei constitutet a mean 3% of the total plasma cell population. They were aways found amongst plasma
cell infiltrates and in close association with small blood vessels. Ultrastructural analysis found no evidence of cellular
membranes separating the individual nuclei in binucleated or multinucleated plasma cells, suggesting that the cells did not
arise from fusion. Some of these plasma cells had a diameter approaching 100 μm, and many were in intimate contact with macrophages.
The demonstration of a few cells with mitotic figures within the infiltrates suggests that the maintenance of plasma cell
numbers in rheumatoid synovium may depend, in part, upon their local proliferation.
Received: 25 August 1997 / Accepted: 2 October 1997 相似文献
73.
采集23名产妇的脐带血和8名正常成人的外周血制备LAK细胞,随机分为4组,在培养第3,5,7,14天,分别利用MTT法和^125I-UdR释放法对4组LAK细胞的增殖力和杀伤活性进行了测定,同时用细胞毒方法测定培养7天的LAK细胞的免疫表型。结果发现,在不同培养时间,脐血来源的LAK细胞增殖力显著高于成人血LAK细胞(P〈0.05或P〈0.01),成人血清组,脐血血清组,红细胞组LAK细胞增殖力有 相似文献
74.
PACAP is a hypothalamic hypophysiotropic factor that acts upon a number of pituitary cells, including gonadotrophs. In the gonadotroph-derived αT3-1 cell line, PACAP acts via PVR1 receptors to stimulate adenylyl cyclase and phosphoinositidase C. PACAP-stimulated cAMP accumulation is inhibited by protein kinase C-activating phorbol esters in these cells and the current work was undertaken primarily to establish whether it is also subject to homologous regulation. In acute experiments, PACAP27-stimulated cAMP accumulation (intracellular plus extracellular) was measured (in the presence of phosphodiesterase inhibitor) both in intact cells and in cell membranes. The peptide increased cAMP accumulation, but initial rates of PACAP27-stimulated cAMP accumulation were reduced to between 10 and 50% within 10 min of stimulation in both cells and membranes. The initial rate of forskolin-stimulated cAMP accumulation was maintained in membranes but not in intact cells (although the deviation from linearity was less pronounced than with PACAP27). Thus, rapid homologous desensitization to PACAP27 occurs in intact αT3-1 cells, but is not entirely receptor specific. Rapid homologous desensitization of PACAP27-stimulated cAMP accumulation also occurred in the presence of a protein kinase C activating phorbol ester, which inhibited cAMP accumulation without altering the kinetics of the PACAP27 effect. Brief pre-treatment (3 min) with PACAP27 also reduced the ability of PACAP27, but not gonadotrophin-releasing hormone, to cause a spike-type elevation of cytosolic Ca2+ concentration (a consequence of phosphoinositidase C activation). In chronic desensitization studies, pre-treatment for 6 h with PACAP27 caused a dose-dependent (IC50 approximately 10 nM) reduction of PACAP-stimulated cAMP accumulation and down regulated cell surface PVR1 receptors (to approximately 50%). Thus, it appears that PACAP27-stimulated (PVR-1 receptor mediated) adenylyl cyclase undergoes rapid homologous desensitization in αT3-1 cells, which is paralleled by homologous desensitization of PACAP27-stimulated phosphoinositidase C activity and involves mechanisms distinct from those underlying heterologous desensitization by phorbol esters. Chronic desensitization of PACAP-stimulated cAMP accumulation and down-regulation of cell surface PVR-1 receptors also occurs in these cells although the receptor loss may not entirely explain the observed desensitization. 相似文献
75.
Mice homozygous for the lpr mutation have B and T cell defects and develop autoantibodies, suggesting that lpr plays a role in their genesis. The lpr defect has been identified as a mutation in the apoptosis-associated Fas receptor (FasR) gene. To begin to define the role of FasR in B cells, we have surveyed FasR expression on B-lineage cells from early progenitors in the bone marrow through their maturation in the periphery. Contrary to some reports, we found that FasR is expressed on B cells at all stages of their development and is highest on germinal center B cells. FasR is not expressed on lpr/lpr-derived cells. These data are consistent with the idea that lpr/lpr mice have an intrinsic B cell defect that may be manifested in developing as well as peripheral B cells. An unexpected finding is that B-1 (CD5) B cells do not constitutively express FasR: FasR becomes detectable on B-1 B cells only after activation. 相似文献
76.
High levels of the soluble form of CD30 molecule in rheumatoid arthritis (RA) are expression of CD30+ T cell involvement in the inflamed joints. 总被引:2,自引:0,他引:2 下载免费PDF全文
R Gerli C Muscat O Bistoni B Falini C Tomassini E Agea R Tognellini P Biagini A Bertotto 《Clinical and experimental immunology》1995,102(3):547-550
The CD30 is a surface molecule expressed by Th2-type lymphokine-producing T cells upon activation. CD30-expressing activated T cells release a soluble form of the molecule, which can be detectable both in vitro and in vivo. In the present study, high levels of soluble CD30 were found in peripheral blood and synovial fluid from patients with RA. However, CD30+ CD3+ cells, either CD4+ or CD8+, were significantly present in synovial fluid, but not in peripheral blood, of RA patients. Serum values of soluble CD30 were higher in active than inactive RA patients and directly correlated with rheumatoid factor serum titres. These data strongly support an involvement of CD30+ T cells in the immune processes of rheumatoid synovitis, and may suggest a relationship between Th2-type cytokine-secreting T cells and the pathological response in RA. 相似文献
77.
78.
置幽门螺杆菌(HP)悬液于含人喉癌上皮细胞(Hep-2)或人胃癌上皮细胞(MKN-28)的培养瓶内,经2h或3.5h培养后观察HP对细胞的粘附情况。结果发现HP能成丛地粘附在上皮细胞表面,少数侵入胞浆内。HP对MKN-28粘附率明显高于对Hep-2细胞者。随菌龄的延长或菌株的变异,HP的粘附作用明显减弱。1%D-甘露糖对HP的粘附性有部分阻抑作用。 相似文献
79.
研究新生仔兔和成兔呼吸道内分泌细胞的形态结构及分布,结果显示在呼吸道有单个物成群的内分泌细胞,单个的内分泌细胞从喉直至肺内导气部粘膜上皮内均可观察到,细胞形态多样,细胞胞质充满大量银染黑色颗粒;成群的内分泌细胞,仅分布于肺的支气管树粘膜上皮内,由几个或几十内分泌细胞组成圆形或卵圆形的小体,细胞胞南内也有大一银染黑色颗粒。 相似文献
80.
G. W. McCAUGHAN C-L. SIAH C. ABBOTT J. WICKSON M. BALLESTEROS G. A. BISHOP 《Journal of gastroenterology and hepatology》1993,8(2):142-145
Dipeptidyl peptidase IV (DPP-IV) is a cell surface ectopeptidase that has been implicated in cell-extracellular matrix interactions, lymphocyte growth and the regulation of biological peptides. Previous studies have shown that immunostaining for DPP-IV and DPP-IV enzyme levels is decreased in hepatoma cells and levels have been correlated with the ability of such cells to adhere in vitro. The aim of this paper was to measure DPP-IV enzyme levels in rat hepatoma cells and to examine whether changes were associated with alterations at the mRNA level. The results indicate a greater than 90% reduction in DPP-IV enzyme levels in two rat hepatoma cell lines, HTC and H35, compared with rat hepatocytes. Enzyme levels of the control enzyme leucine aminopeptidase (LAP) were not decreased. mRNA studies indicated that these changes were associated with similar reductions in rat DPP-IV mRNA. It is concluded that DPP-IV is markedly reduced at the protein, enzyme and mRNA levels in rat hepatoma cells. The significance of these changes is unclear but may lead to decreased extracellular matrix interactions by such cells. 相似文献