THAP11通过抑制p53的泛素化影响食管癌细胞的增殖和凋亡

目的：探讨死亡相关蛋白(thanatos-associated protein,THAP)11对食管癌细胞增殖和凋亡的影响及其潜在 的机制。方法：采用蛋白质印迹法检测人食管上皮细胞Het-1A和人食管癌细胞(Eca109,TE-1和Ec 9706)中THAP11的 表达。将食管癌TE-1细胞分为空白对照(NC)组、阴性对照(LV-LC)组、TRA P11(LV-TRA P11)组,按分组处理细胞后, 采用MTT 法检测细胞活力,流式细胞术检测细胞凋亡,caspases试剂盒检测caspase-3和caspase-9的活性。采用体外泛素 化实验检测TE-1细胞的p53泛素化水平。结果：与Het-1A细胞相比,食管癌细胞中THAP11的表达显著下降(P<0.05)。 LV-THAP11转染食管癌细胞后,细胞活力降低(P<0.05),凋亡率升高(P<0.05),caspase-3和caspase-9活性升高(P<0.05)。 THAP11能够提高食管癌细胞中p53蛋白的表达(P<0.05)。与LV-LC组相比,转染THAP11后食管癌细胞中p53的表达上调 (P<0.05),MDM2调节的p53的泛素化也被抑制。结论：THAP11通过抑制p53的泛素化抑制食管癌细胞的增殖,促进食管 癌细胞的凋亡。     

Role of Laryngeal Movement and Effect of Aging on Swallowing Pressure in the Pharynx and Upper Esophageal Sphincter

Objectives Describe contribution of laryngeal movement to pressure changes at the upper esophageal sphincter (UES) and the effect of aging on the swallowing function. Study Design Manofluorography on 56 nondysphagic adults divided into three age groups: the 21‐ to 31‐year‐old group (n = 32), the 61‐ to 74‐year‐old group (n = 12) and the 75‐ to 89‐year‐old group (n = 12). Analyses of the bolus transit time, the amplitudes and durations of pharyngeal pressures, the timing of a pressure fall at the UES and the laryngeal movements. Methods Intraluminal strain‐gauge sensors recorded pressure changes in the oropharynx, hypopharynx and the UES. Motion pictures of the videotapes were fed into a personal computer, and movements of the hyoid bone were measured in both the horizontal and vertical directions as an indication of laryngeal movement. Results In 26‐ and 70‐year‐old men with calcification of the thyroid cartilage, it was determined that the larynx and hyoid bone moved in consonance until the end of the rapid hyoid movements in both the superior and anterior directions. In the 21‐ to 31‐year‐old group, the magnitude of the pressure fall at the UES was maximal before or almost at the same time as the bolus arrival, in preparation for smooth passage of the bolus from the pharynx to the esophagus. The rapid superior movements of the hyoid bone started significantly early as compared with its anterior movements (P = .0001). The rapid anterior movements of the hyoid bone started simultaneously with the pressure fall at the UES. In the elderly, all segmental transit times were significantly increased. The timing of the pressure fall at the UES was significantly delayed and the UES pressure reached its minimum value after arrival of the bolus at the UES. The minimum pressure at the UES increased to a significantly positive value. The rapid anterior movements of the hyoid were significantly delayed, suggesting that this delay causes the delay in the pressure fall at the UES. Conclusions The rapid superior and anterior movements of the hyoid bone are considered to start at the same time as those of the larynx. In the young group, it is suggested that superior laryngeal movement protects the lower airway prior to the anterior laryngeal movement, causing the pressure fall at the UES to enable the passage of a bolus into the UES. In the elderly, smooth passage of the bolus from the pharynx to the esophagus is hindered and the system that prevents aspiration is rendered inefficient by changes in the swallowing pressures and laryngeal movements with aging.     

胸导管结扎预防食管癌术后乳糜胸的临床研究

目的　寻找减少或消除食管癌切除术后乳糜胸发生率的有效方法。方法　随机选取 45例 (A组 )食管癌患者 ,在食管癌切除加胃 食管残端吻合时 ,一期行预防性低位胸导管结扎术 (第 8胸椎水平以下 ) ;另 92例 (B组 )作对照组 ,仅做食管癌切除加胃 -食管残端吻合术。结果　两组患者在术后乳糜胸发生率 (A :1/ 45 ;B :9/ 92 ,P =0 0 3)、术后 48h胸腔引流量〔第 1个 2 4h :A :(2 5 4 33± 45 15 )ml,B :(4 93 6 1± 76 6 7)ml;第 2个 2 4h :A :(141 11± 2 5 5 3)ml,B :(32 3 39± 5 8 17)ml;P <0 0 5 )〕、3天内拨管率 (A :94 78% ;B :6 2 2 1% ,P =0 0 0 1)、并发症 (A :3/ 45 ,B :0 / 92 ,P =0 0 34)以及随访锁骨上淋巴结转移 (A :0 / 45 ,B :9/ 92 ,P =0 0 3)等方面有统计学意义。结论　术中行预防性低位胸导管结扎术可明显减少甚至消除食管癌患者术后乳糜胸的发生。     

良性上消化道疾病的食管动力学变化

目的：探讨良性上消化道疾病患者食管动力学的变化情况。方法：对３２例良性上消化道疾病患者进行食管测压检查。结果：３４．３８％的病例ＬＥＳＰ减低,８７．５％出现吞咽后同不收缩,５３．１３％出现自发性同步收缩,２８．１３％正常需动收缩完全消失,５０％出现远端食管平均收缩压力减低,５６．２５％出现远端食管低压收缩。结论：本组病例多有远端食管运动功能障碍,以吞咽后同步收缩为主,其与反流性食管炎以及一些临床症     

蒙药广枣总黄酮对大鼠离体心脏功能的影响

目的：探讨广枣总黄酮(TFC)对心功能的影响。方法：采用Langendorff灌流法．用多导生理记录仪测定大鼠离体心脏的心率、心肌收缩力及心电图的变化。结果：TFC使正常灌流的大鼠离体心脏心电图的P—R问期延长．心率减慢．心肌收缩力增强;对缺氧灌流所导致的心率减慢和心脏收缩力减弱均呈明显的对抗作用。结论：TFC可改善心功能。     

奥曲肽及垂体后叶素治疗食管胃底静脉曲张破裂出血的效果观察

①目的 比较奥曲肽和垂体后叶素治疗肝硬化食管胃底静脉曲张破裂出血的临床效果。②方法 将９２例（１０５次）食管胃底静脉曲张破裂出血病人随机分为奥曲肽组（５０例５８次）和垂体后叶素组（４２例４７次）,分别给予奥曲肽和垂体后叶素治疗,每隔０．５ｈ冲洗胃腔吸取胃液１次,比较两组止血率。③结果 奥曲肽组和垂体后叶素组８ｈ内止血率分别为６０．３％,１７．０％,差异有极显著性（Ｘ＾２＝２０．１５,Ｐ〈０．０１）     

心脏停搏液预处理对大鼠离体心脏缺血性挛缩及再灌注冠脉流量的影响

目的　观察心脏停搏液（ 简称停搏液）预处理对大鼠离体心脏缺血性挛缩的发生及再灌注冠脉流量的影响。方法　选用ＳＤ大鼠离体Ｌａｎｇｅｎｄｏｒｆｆ 逆行灌注模型,观察应用停搏液时心脏缺血性挛缩的发生时间和幅度以及再灌注心脏冠脉流量的改变。结果　停搏液预处理显著增加了心脏缺血再灌注期间的冠脉流量,显著延迟了缺血期间心肌挛缩的发生时间,并降低其幅度。结论　停搏液预处理减轻缺血心肌挛缩的程度,可能是其心脏缺血再灌注损伤的保护作用的机制之一。     

Study on genetic polymorphisms of DCC gene VNTR in esophageal cancer

The abeted in Colo~ cancer gene (DCC) is re~ as a susceptibility gene in colol'eCtal cancer, whichis located on chlomesome 18qZI. 2. Ihactivation of thisgene may Play an i~ role dndng some Pimessessuch as ~ p~ssion and metastasis. With its ~allycloned, in lop, casinger et alllJ found that a vocablenumber tandem repeat (VNTR) is located wick an intwOf the DCC gene, its lepeat act is 2 hp,the core seqUence of (TA)n ~ numbers ~ 10 -- to tinies, whichis an ideal genetic rnalker. Esophageal…     

食管癌APC/MCC基因杂合缺失的研究

目的 研究ＡＰＣ／ＭＣＣ基因杂合性缺失（ＬＯＨ）与食管癌发生发展的关系。方法 多聚酶锭反应技术及限制性片段长度多态现象对４６例食管癌ＡＰＣ和ＭＣＣ基因的ＬＯＨ进行了分析。结果 食管癌ＡＰＣ和ＭＣＣ基因ＬＯＨ阳性表达分别为２９．０％和３３．３％。结论ＡＰＣ、ＭＣＣ基因的ＬＯＨ是食管癌的常见改变,可能参与了食管癌的发生。     

不同浓度乙醇灌流液对蟾蜍离体心脏活动影响的比较分析

用不同浓度乙醇灌流液灌流蟾蜍离体心脏,观察其对离体心脏心率、心肌收缩力和心输出量的变化。结果显示：心率减慢,但在１０ ｍ ｍｏｌ／Ｌ 稍有加快（ Ｐ ＞ ０ ．０５） ,浓度在２０ ｍ ｍ ｏｌ／Ｌ 以上时减慢才明显（ Ｐ ＜ ０ ．０５） ;心肌收缩幅度下降,其表现浓度越大下降越明显（ Ｐ ＜ ０ ．０５ ～０ ．０１） ;心输出量在１０ ｍ ｍｏｌ／Ｌ、１５ ｍ ｍｏｌ／Ｌ 时略有增加,超过３０ ｍ ｍｏｌ／Ｌ 以后心输出量下降,但不明显（ Ｐ ＞ ０ ．０５） 。表明乙醇过量有减慢心率和抑制心肌收缩力的作用,而对输出量影响不大。