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排序方式: 共有470条查询结果,搜索用时 15 毫秒
1.
建立一种粪便细菌学检验的新方法--肠道菌谱分析(初步报告) 总被引:3,自引:0,他引:3
目的 建立一种以粪便标本细菌菌谱分析为主要内容的细菌学检验方法。方法 取粪便标本直接涂片做革兰染色,镜检观察标本中细菌数量、种类及比例,并进行显微照相;对粪便标本进行菌群培养,分离病原菌,并与镜检结果进行对照;将照片进行分类。建立菌群失调和病原菌感染的图谱。同时以正常大便菌谱作对照。结果 因菌群失调导致的腹泻。直接镜检表现为细菌数量和种类普遍减少,或某一类细菌增多或减少,或呈单一种类细菌;由某种病原菌引起的腹泻,直接涂片多数只能见到革兰阴性杆菌,且细菌数量少或极少,一般均与培养结果相符。结论 粪便细菌菌谱分析能直接或间接为临床提供腹泻患者的肠道微生态资料,尤其能准确地提示因各种原因造成菌群失调而导致的腹泻,为临床诊断和制定治疗方案提供快捷、科学的依据。 相似文献
2.
J Slusarczyk B G Hansson E Nordenfelt K Krawczyński S Karwowska J Knap 《Journal of medical virology》1985,15(2):105-112
The excretion of hepatitis A virus (HAV) in stools from 30 patients with clinically overt hepatitis A infection on the day of their admission to the hospital was determined and compared with the dynamics and values of biochemical indices of hepatocyte injury as well as with the immune response to HAV. Virus was found in 16 out of 30 stools (53%) collected within 1 week after the appearance of clinical symptoms. In sera obtained on the day of hospitalization both IgM and IgA anti-HAV were detected in all of the 30 patients, while IgG anti-HAV were found in 20 (67%). There was a correlation between HAV excretion and increasing SGPT upon admission to hospital, while the level of SGPT or bilirubin as well as presence or absence of IgG anti-HAV did not correlate with excretion of HAV. HAV from stools was characterized morphologically and physicochemically. The majority of particles visualized by immune electron microscopy had electrondense appearance, while electron-lucid particles were only occasionally encountered. Isopycnic banding of HAV in CsCl revealed a broad range of densities with HAV activity. Rebanding of pooled fractions containing HAV revealed peak amounts of the virus in fractions with densities 1.32-1.33 gm/cm3. 相似文献
3.
Isolation of exfoliated colonocytes from human stool as a new technique for colonic cytology 总被引:4,自引:0,他引:4
Bandaletova T Bailey N Bingham SA Loktionov A 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2002,110(3):239-246
Cell exfoliation in the gut is an important cell renewal mechanism. To approach its investigation we applied a novel immunomagnetic technique for isolation of exfoliated cells from human stool. Exfoliated colonocytes were isolated from 168 stool samples. The cells were assessed microscopically using conventional stains and immunohistochemistry. The technique allowed us to obtain well-preserved colonocytes displaying characteristic features of well-differentiated colonic epithelium and positive immunostaining for cytokeratin 5/8. No mucin-producing cells were found. Exfoliated cells did not produce inducible nitric oxide synthase, albeit cultured colon carcinoma cells HT-29 analysed in parallel showed strong immunostaining. Analysis of exfoliated cell numbers in consecutive stool samples from the same subjects revealed considerable interindividual variation. Overall exfoliated colonocyte numbers were relatively low, isolation being unaffected by addition during the procedure of excessive amounts of HT-29 cells. Apoptosis was extremely rare among exfoliated colonocytes. Well-preserved exfoliated colonocytes can be consistently isolated from human faeces using a simple procedure. Our findings suggest that the actual process of cell exfoliation in the human colon may be much less intense than is generally accepted. Exfoliated cell isolation from human stool constitutes a convenient non-invasive approach that can be used for diagnostic and research purposes. 相似文献
4.
Anselme Millogo Ahmed Loukil Coralie LOllivier Diakourga Arthur Djibougou Sylvain Godreuil Michel Drancourt 《Emerging infectious diseases》2021,27(6):1758
Mycobacterium leprae was detected by optical microscopy, fluorescent in situ hybridization, and molecular detection in feces collected for the diagnosis of Entamoeba coli enteritis in a leprosy patient in Burkina Faso. This observation raises questions about the role of fecal excretion of M. leprae in the natural history and diagnosis of leprosy. 相似文献
5.
洞庭湖垸外血吸虫病易感地带野粪分布及与感染螺密度相关研究 总被引:4,自引:0,他引:4
目的探讨洞庭湖垸外血吸虫病易感地带距大堤不同距离湖洲野粪分布特点及与感染螺密度的关系。方法1987~1994年,选择洞庭湖区30个村外洲进行了观察。结果,野粪污染湖洲以牛、猪、人为主,离大堤愈近,密度愈高。牛粪在各距离中均占绝大多数,且离堤愈远比例愈高;猪粪集中分布在距大堤300m内;人粪呈散在分布。结论<300m,各年感染螺密度均>0.005只/0.11m2,属一级易感地带,且与野粪密度有直线相关关系;然两者均呈逐年下降之势。300m外,年间虽有变化,但无规律 相似文献
6.
7.
H. Kitajima Y. Sumida R. Tanaka N. Yuki H. Takayama M. Fujimura 《Archives of disease in childhood. Fetal and neonatal edition》1997,76(2):F101
AIM—To investigate the colonisation with Bifidobacterium breve of the bowels of very low birthweight (VLBW) infants.METHODS—The adverse effects of B breve were examined in 66 VLBW infants (preliminary study). A prospective randomised clinical study of 91 VLBW infants was also completed and these infants were followed up for three years. Precise viable bacterial counts of serial stool specimens were examined for the first eight weeks after birth in 10 infants. The colonisation rates of administered bacteria were examined using immunohistochemical staining of stool specimens with a B breve specific monoclonal antibody.RESULTS—In the preliminary study there were no side effects attributable to the bacteria. Immunohistochemical staining of stool specimens showed that the colonisation rates of the administered bacteria were 73% at 2 weeks of age, but only 12% in the control group. Early administration of B breve significantly decreased aspirated air volume from the stomach and improved weight gain.CONCLUSIONS—B breve can colonise the immature bowel very effectively and is associated with fewer abnormal abdominal signs and better weight gain in VLBW infants, probably as a result of stabilisation of their intestinal flora and accelerated feeding schedules. 相似文献
8.
Incontinenceofurineandstoolisadiseasethatpatientcannotcontrolurinationanddefeca-tionatastateofclearconsciousness.Alotoffactorsofsocialpsychologymaybringaboutthisdisease.51casesofthediseasecausedbyexcessivesorrowandfrightwerestudiedandtreatedwithacupunctur… 相似文献
9.
10.
《Scandinavian journal of gastroenterology》2013,48(7-8):831-836
Abstract Objective. To examine whether a probiotic strain, Lactobacillus gasseri OLL2716 (LG21), can protect the gastric mucosal integrity from aspirin using urinary sucrose excretion (USE) test. Materials and methods. In the study using high-dose aspirin, the USE tests were carried out in 29 volunteers before and after LG21 treatment for 4 weeks. In the study using patients undergoing low-dose aspirin therapy, USE tests were performed in 37 subjects who took LG21 for 16 weeks. Stool occult blood was examined by the guaiac method. Results. In the former study, the elevation in the USE value after aspirin loading significantly decreased after LG21 treatment (Median ± SD; 0.244 ± 0.237 vs. 0.208 ± 0.112%, p = 0.018). In the latter study, the USE value significantly decreased in the period with LG21 treatment (p = 0.033), while no significant difference was found in the period without LG21 (p = 0.113). The number of positive occult blood tests decreased during LG21 treatment. Conclusions. The regular ingestion of LG21 may protect the integrity of the gastric mucosal permeability against aspirin. 相似文献