短期胰岛素泵治疗对2型糖尿病患者血内脏脂肪素的影响

目的比较短期胰岛素泵治疗对不同病程2型糖尿病患者血内脏脂肪素（visfatin,VF）的影响。方法选择2型糖尿病患者100例,根据病程分为观察组40例（初诊病程〈1年）和对照组60例（病程1～10年）。两组均使用胰岛素泵持续皮下输注超短效胰岛素（诺和锐）2周。比较两组空腹血糖（FBG）、餐后2h血糖（2hFBG）、糖化血清白蛋白（GA）、空腹内脏脂肪素（VF）、空腹C肽（C-P）等变化。结果两组FPG、2hFPG、GA、C-P、空腹内脏脂肪素差异均有统计学意义（t=3.573,P〈0.05）;两组治疗前后变化量比较具有统计学意义（t=2.784,P〈0.05）,VF与FBG、2hFPG及GA成正相关。结论早期胰岛素泵强化治疗能够很好控制血糖水平,对改善β细胞功能及胰岛素抵抗有着重要的作用,而这种作用在1年以上病程的患者中表现相对不明显。     

冠心病患者 Visfatin mRNA 基因表达及相关因素的研究

目的：探讨Visfatin（内脏素）基因在冠心病腹部皮下及大网膜的脂肪组织中表达情况及相关因素的分析,从而为冠心病防治提供一种新的、切实可行的新方法。方法：根据既往冠心病的病史的不同,将100例胆石症择期手术患者分为冠心病组50例,对照组50例。用RT-PCR方法检测两组的大网膜与皮下脂肪组织Visfatin mRNA的表达水平,并测定空腹胰岛素（ FINS）、血脂和胰岛素抵抗指数（ HOMA-IR）等指标。结果：①冠心病组血清Visfatin浓度高于对照组;②冠心病组者腹部皮下和网膜脂肪组织中的Visfatin表达高于对照组;结论：Visfatin在冠心病中表达明显增高,可能是冠心病发病独立危险因素。     

胰升血糖素样肽-1促进内浆网应激脂肪细胞内脏脂肪素的表达

目的 探讨胰升血糖素样肽-1（GLP-1）对内质网应激状态下脂肪细胞内脏脂肪素表达的调节作用。 方法 3T3-L1分化成熟的脂肪细胞,经Tg诱导建立内质网应激模型。脂肪细胞经Tg和/或GLP-1孵育后,收集细胞用于总RNA的提取,收集细胞液用于ELISA分析。定量PCR检测内脏脂肪素转录水平,ELISA检测其分泌水平。内质网应激发生通过测定sXBP-1表达水平增加来判断。使用细胞核因子κB（NF-κB）特异阻断剂PDTC预处理30 min,观察NF-κB在GLP-1调节内脏脂肪素表达中的作用。 结果 Tg诱导后sXBP-1表达增加,内脏脂肪素表达下降并呈剂量依赖性;同时给予GLP-1处理后,内脏脂肪素表达增加;PDTC降低内脏脂肪素基础水平,并未阻断GLP-1作用。 结论 GLP-1提升内质网应激状态下脂肪细胞内脏脂肪素的表达。     

Serum visfatin levels, adiposity and glucose metabolism in obese adolescents

Objective: Visfatin, an adipokine, has insulin-mimetic effects. The main determinants of both the production and the physiologic role of visfatin are still unclear. The aim of this study is to determine the relation of serum visfatin to adiposity and glucose metabolism.Methods: 40 pubertal adolescents (20 females, 20 males; age range: 9-17 years) with exogenous obesity and 20 age- and sex-matched healthy adolescents (10 females, 10 males) were enrolled in the study. Oral glucose tolerance test (OGTT) was performed in the obese group. Serum glucose, insulin and visfatin levels were analyzed in the fasting state in the controls and at 0, 60 and 120 minutes during the OGTT in the obese group.Results: The obese group had higher serum visfatin levels than the control group [11.6 (3.3-26) ng/mL vs. 7.5 (3.3-10.5) ng/mL, p<0.001]. Visfatin levels were correlated positively with body mass index, waist/hip ratio, insulin, and homeostasis model assessment for insulin resistance and negatively with glucose/insulin ratio in the combined group (obese subjects plus controls). Visfatin levels were essentially similar in obese subjects with and without insulin resistance (p>0.05). Serum visfatin levels did not change at 60 and 120 minutes of the OGTT compared to the baseline levels (p>0.05).Conclusions: Serum visfatin levels are elevated in obese adolescents and do not change with acute changes in glucose metabolism. Visfatin levels are related with adiposity and glucose metabolism parameters. However, the role and contribution of adiposity and glucose metabolism to the circulating visfatin levels in obese patients remain to be explored. Conflict of interest:None declared.     

血浆内脂素水平与宁夏回、汉族2型糖尿病的相关性

目的探讨血浆内脂素（Visfatin）水平与宁夏回、汉族2型糖尿病的相关性。方法采用ELISA法测定所有受试者血浆内脂素水平,同时检测空腹静脉血糖（FPG）、餐后2h血糖（2hPG）、糖化血红蛋白（HbA1c）、甘油三酯（TG）、总胆固醇（TC）等指标,测量并计算体重指数（BMI）、腰臀围比（WHR）。结果回、汉族2型糖尿病患者血浆内脂素水平均高于健康体检者;回族2型糖尿病组血浆内脂素水平与体重指数（BMI）、腰臀比（WHR）、FPG呈正相关,汉族2型糖尿病组血浆内脂素水平与WHR、FPG、2hPG、HbA1c、TG呈正相关。结论血浆内脂素与2型糖尿病及肥胖相关,可能在2型糖尿病的发生、发展中起一定作用。     

冲压发动机C/SiC喷管承压失效研究

目的 了解成人生长激素缺乏症(GHD)患者体内的瘦素(leptin)、脂联素(ADP)、内脂素(visfatin)水平变化以及与体质指标、血脂及各种激素指标的关系.方法 纳入成人GHD患者40例(男19例)作为观察组,健康人36例(男18例)作为正常对照组.收集两组的基本资料,包括身高、体质量、腰围、臀围、血脂、甲状腺激素、生长激素、胰岛素、性激素等指标.采集空腹静脉血标本进行血清瘦素、脂联素、内脂素水平测定.结果 GHD组腰臀比、胆固醇(CHOL)、甘油三酯(TG)、低密度脂蛋白-胆固醇(LDL-C)、瘦素、脂联素、内脂素水平均高于正常对照组(P＜0.05);GHD组生长激素(GH)、胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-3(IGFBP-3)、硫酸脱氢表雄酮(DHEA-S)水平均低于正常对照组(P＜0.05).②经多因素logistic回归分析得出,内脂素水平升高的人群患成人GHD的风险增高(OR=1.430,P＜0.05).结论 内脂素水平升高的人群患GHD的风险较正常人群增高.     

Umbilical cord blood and maternal visfatin (PBEF/NAMPT) concentrations in preterm birth with and without preterm premature rupture of membranes

Objectives: The aim of the study is to investigate differences in visfatin concentrations between mothers with term and preterm birth (PTB) and between mothers who delivered within seven days and after more than seven days following admission for PTB/preterm premature rupture of membranes (PPROMs).

Methods: Maternal peripheral blood and cord blood were collected from 56 mothers with PTB (31 with PPROM) and 71 mothers with term delivery (three with PPROM).

Results: Maternal visfatin concentration was significantly higher for given gestational age in PTBs compared to term deliveries (p?=?.021) and also in mothers who delivered within seven days after admission for PTB or PPROM, compared to those who delivered after more than seven days (p?=?.027; p?=?.039). Cord blood visfatin concentration was found to be decreased in preterm compared to term infants (p?=?.007).

Conclusions: Visfatin in both maternal and fetal circulation may play an important role in the pathogenesis of PTB/PPROM and could be used to distinguish between women who will deliver in a short period of time after clinical presentation of PTB/PPROM and those who deliver later. Nevertheless, additional research is necessary in order to identify its direct involvement in PTB/PPROM.     

血清内脂素及氧化应激状态评价在妊娠糖尿病患者病情评估中的意义

目的探讨血清内脂素及氧化应激状态评价在妊娠糖尿病患者病情评估中的意义。方法选择妊娠糖尿病患者68例为糖尿病组,对照组选择非糖尿病孕妇60例。人选后分别检测两组对象血清内脂素、空腹血糖、胰岛素抵抗指数、丙二醛、过氧化氢酶、超氧化物歧化酶、谷胱甘肽并计算体重指数。结果糖尿病组空腹血糖、胰岛素抵抗指数,内脂素较非糖尿病组差异有统计学意义（P〈0．05）。糖尿病组的MDA、CAT较非糖尿病组有显著性增高（P〈0．05）,糖尿病组SOD水平较非糖尿病组有极显著性下降（P〈0．01）,GSH较非糖尿病组有显著性下降（P〈0．05）。空腹血糖与内脂素、MDA呈显著正相关（P〈0．05）,空腹血糖与SOD、GSH呈显著负相关（P〈0．05）;胰岛素抵抗指数与内脂素、MDA呈显著正相关（P〈0．05）,胰岛素抵抗指数与SOD、GSH呈显著负相关（P〈0．05）。结论血清内脂素及氧化应激状态评价可有效反映妊娠糖尿病患者病情严重程度,对判断病情进展具有重要意义。     

2型糖尿病合并危重症患者血糖监测及治疗观察

目的探讨2型糖尿病（T2DM）合并危重症患者血糖监测的意义及血糖控制对危重症患者的意义。方法选择T2DM并内科危重症患者76例,随机分为对照组和治疗组。对照组采用胰岛素皮下注射控制血糖,治疗组采用胰岛素泵控制血糖水平。人选后分别检测两组对象空腹血糖、三餐后血糖、血清内脂素、空腹C肽、糖化血红蛋白及餐后2hC肽并计算病死率。结果治疗组胰岛素用量较对照组有显著性下降（P〈0．05）,治疗组血糖控制良好者较对照组有显著性升高（P〈0．05）,对照组血糖控制差者较治疗组有显著性升高（P〈0．01）。治疗组低血糖发生率及内脂素水平较对照组有显著性下降（P〈0．05）。治疗后治疗组血糖波动及病死率较对照组有显著性下降（P〈0．05）。结论采用胰岛素泵治疗可积极的控制血糖并降低血糖波动,可以减少T2DM合并危重症患者病死率。