河南地区寻常型银屑病389例临床分析

银屑病是一种常见并易复发的慢性炎症性皮肤病。本研究立足河南,以中医证候为切入点,探讨银屑病发病与患者的基本情况及影响因素的关系,分析证候分布规律,对更深入认识本病,指导银屑病临床预防和治疗具有重要意义。     

窄谱蓝光联合1%克林霉素磷酸酯凝胶治疗寻常痤疮疗效观察

目的观察窄谱蓝光联合外用克林霉素磷酸酯凝胶治疗寻常痤疮的疗效。方法 110例痤疮患者随机分为两组,治疗组58例,采用窄谱蓝光照射联合外用1%克林霉素磷酸酯凝胶治疗;对照组52例,外用1%克林霉素磷酸酯凝胶治疗。结果治疗组和对照组有效率分别为75.9%和25.0%,治疗组疗效和有效率高于对照组(P<0.01)。结论窄谱蓝光照射联合外用1%克林霉素磷酸酯凝胶治疗痤疮疗效优于单用克林霉素磷酸酯凝胶。     

A pilot methodology study for the photographic assessment of post‐inflammatory hyperpigmentation in patients treated with tretinoin

Background Post‐inflammatory hyperpigmentation (PIH) is a common occurrence in patients with acne vulgaris, particularly in those with skin of colour. Aims A previous study has demonstrated the benefit of tretinoin (retinoic acid) in the treatment of PIH; however, there is currently no standard protocol to evaluate change in PIH following treatment. Based on these findings, we performed a pilot, exploratory, blinded, intraindividual‐controlled methodology study that consisted of a photographic assessment protocol with facial mapping. Materials and methods The study was based on a secondary analysis of a phase 4, community‐based trial of 544 acne patients who were treated with tretinoin gel microsphere 0.04% or 0.1%. Only patients with Fitzpatrick types III–V (skin of colour) were included in the study; subjects with Fitzpatrick skin type VI were excluded because the photographic assessment did not allow for proper evaluation. Results Despite the small number of subjects evaluated (n = 25), the results revealed consistent assessment of improvement in PIH between two independent graders (weighted κ = 0.84). Conclusion Further study with a larger population is recommended to validate the accuracy of this method.     

窄谱中波紫外线联合中药药浴治疗寻常型银屑病疗效观察及对血管内皮细胞生长因子影响

目的:观察中药药浴联合窄谱中波紫外线(NB-UVB)治疗寻常型银屑病的疗效及治疗前后血清细胞因子浓度改变,探讨治疗机制。方法:32例寻常型银屑病患者中药药浴后,给予NB-UVB照射,每周3次,共40次。用酶联免疫吸附试验检测32例患者治疗前后血管内皮细胞生长因子(VEGF)水平。结果:32例患者治疗后痊愈11例,临床有效率为90.6%。32例患者治疗前VEGF血清水平较健康对照者水平高,照射后显著下降。结论:中药药浴联合NB-UVB照射治疗银屑病疗效好,不良反应少,VEGF在寻常型银屑病中发挥重要作用。     

炎琥宁注射液治疗寻常型银屑病前后外周血T细胞亚群的变化

目的：探讨寻常型银屑病患者炎琥宁注射液治疗前后外周血CD3^＋，CD4^＋，CD8^＋ T细胞变化情况。方法：40例患者中予炎琥宁注射液240mg加入0．9％生理盐水250mL～500mL中静滴，1次／d；内服中药银花虎杖饮（加减），1剂／d，分2次服；苦豆子油外用，2次／d，治疗4周。治疗前及治疗后检测患者外周血CD3^＋，CD4^＋，CD8^＋ T细胞。选择门诊健康体检者40例作为对照组。结果：治疗组有效率为85％，CD3^＋，CD4^＋，CD8^＋ T细胞以及CD3^＋/CD4^＋比值与治疗前比较差异有统计学意义（P〈0．01）。结论：炎琥宁注射液对寻常型银屑病疗效肯定，其在改善症状的同时亦能纠正患者外周血T细胞及其亚群的失衡状态。     

Human VAT-1: a calcium-regulated activation marker of human epithelial cells

Background and aims Human VAT-1 (hVAT-1) is a homologue of the synaptic vesicle membrane protein of Torpedo californica. Its coding gene is located near the BRCA1 locus and thus hVAT-1 may be linked to an inherited predisposition to breast and ovary cancer. However, the hVAT-1 protein expression pattern in normal epithelial tissues such as skin, mammary gland and ovary, as well as in tumours of the mammary gland and ovary, has not been studied.Methods To address this issue, an immunohistological analysis of biopsies of normal epidermis and lesional epidermis of bullous pemphigoid and pemphigus vulgaris patients was undertaken.Results hVAT-1-expression was observed in basal keratinocytes of lesional epidermis of bullous pemphigoid patients but not in normal epidermis or in lesional epidermis of pemphigus vulgaris patients. Moreover, hVAT-1 expression in HaCaT cells was found to be calcium-dependent. Normal and malignant mammary and ovary epithelium were found to be hVAT-1-negative.Conclusions Our results indicate that hVAT-1 exerts a specific function in keratinocyte physiology, in particular in calcium-regulated processes, with no evident deregulation in malignancies of the breast and ovary.Abbreviations BRCA1 Breast-related cancer antigen 1 - DMEM Dulbecco's modified Eagle's medium - EDTA Ethylenediamine tetraacetate - ELISA Enzyme-linked immunosorbent assay - FCS Fetal calf serum - GAPDH Glyceraldehyde-3-phosphate dehydrogenase - GST Glutathione-S-transferase - HRP Horseradish peroxidase - hVAT-1 Human VAT-1 - NHEK Normal human epidermal keratinocytes - PBS Phosphate-buffered saline - TBS Tris-buffered saline - VAT-1 Vesicle-amine-transport protein-1     

夏枯草及抗炎一号注射液经肝动脉联合灌注治疗大鼠肝癌作用机制的初步探讨

目的:探讨夏枯草及抗炎一号注射液经肝动脉联合灌注治疗大鼠肝癌的作用机制。方法:建立30只二乙基亚硝胺(DEN)诱发大鼠晚期原发性肝癌模型,随机分为中药组、化疗组和盐水组,每组各10只。经胃十二指肠动脉至肝固有动脉分别灌注生理盐水0.5ml、夏枯草注射液0.6g/kg 抗炎一号注射液0.45g/kg、顺铂(DDP)4mg/kg 羟基喜树碱(HCPT)1.5mg/kg 5-氟尿嘧啶(5-FU)34mg/kg。应用流式细胞仪(FCM)检测治疗后各组大鼠肝癌细胞的凋亡情况。结果:盐水组、化疗组和中药组均存在不同程度的细胞凋亡现象,与盐水组比较,中药组和化疗组肝癌细胞凋亡百分率均显著增高(P<0.01),但化疗组和中药组之间无显著性差异(P>0.05)。结论:夏枯草与抗炎一号注射液可诱导大鼠肝癌细胞凋亡,其程度与化疗药物相似。     

通俗环毛蚓的化学成分研究

目的：对中药通俗环毛蚓的化学成分进行分离鉴定。方法：乙醇、乙醚等溶剂提取物，用红外光谱仪和气相色谱-质谱联用方法以及氨基酸分析仪等技术鉴定化合物。结果：在通俗环毛蚓中测定有琥珀酸、18种脂肪酸和20种游离氨基酸。结论：通俗环毛蚓中的脂肪酸可能是其活性成分之一；另外其总游离氨基酸含量较高（含量为8.629%）。     

清肺祛脂方治疗寻常痤疮的临床研究

目的验证清肺祛脂方治疗寻常痤疮的临床疗效和作用机理.方法观察皮肤损害、记录个数、测量皮脂溢出率(吸烟纸法).结果通过临床观察,清肺祛脂方总有效率为90%,跟对照组无显著差异.结论清肺祛脂方是临床治疗寻常痤疮的有效药物,抑制皮脂溢出率是其作用机理之一.