^90Sr-^90Y敷贴与联合瘢痕内切除术治疗大面积病理性瘢痕的疗效

目的观察对比单纯^90Sr-^90Y敷贴和瘢痕内切除术后^90Sr-^90Y敷贴治疗大面积病理性瘢痕的疗效。方法选取临床确诊的大面积病理性瘢痕患者158例,共196块病理性瘢痕,按瘢痕厚度分为A、B、C 3组,每组患者再采用简单随机法分为两部分,分别接受单纯^90Sr-^90Y敷贴治疗（102块）和瘢痕内切除术并^90Sr-^90Y敷贴治疗（94块）,治疗结束后2年评价疗效,行Ridit分析。结果瘢痕内切除术并^90Sr-^90Y敷贴的疗效明显优于单纯^90Sr-^90Y敷贴治疗（R=0．428和0．578,F=92．6,P〈0．01）,前者疗效不受瘢痕厚度的影响,后者疗效则明显受瘢痕厚度影响。结论对于瘢痕表面积和厚度较大的病理性瘢痕患者。宜采用瘢痕内切除术并^90Sr-^90Y敷贴治疗。     

含锶交联藻酸钠凝胶对前成骨细胞黏附和增殖的影响

目的 利用SrCl₂作为交联剂,合成含锶交联新型藻酸凝胶,检测含锶藻酸凝胶的理化性质,并观察细胞于凝胶中的黏附、增殖情况。方法 按钙离子和锶离子比例,分别制备5组终浓度为4 g/L的电解液,分别与藻酸钠溶液交联。检测各组凝胶的压缩模量、溶胀率及锶离子析出浓度。将种子细胞MC3T3-E1接种于凝胶表面后,观察并行 FDA/PI 细胞染色。采用GraphPad Prism 6.0软件包对数据进行统计学分析。结果 藻酸盐凝胶在双蒸水及PBS中,溶胀率随Sr²⁺浓度增高而增大;压缩模量检测发现,C组凝胶强度显著高于其他各组。ICP显示,各组溶液的锶离子浓度逐渐降低。扫描电镜观察发现,凝胶孔隙随Sr²⁺浓度增加而增大。显微镜及FDA/PI染色均发现,种子细胞在C组凝胶表面的增殖明显改善。结论 当钙离子和锶离子等比例混合时,藻酸盐凝胶的细胞黏附和增殖性能明显改善,可作为一种新型可注射骨组织工程支架。     

90Sr-90Y敷贴联合普萘洛尔治疗婴幼儿大面积皮肤血管瘤的临床观察

目的 探讨90Sr-90Y敷贴联合普萘洛尔治疗婴幼儿大面积皮肤血管瘤的临床疗效.方法 将39例婴幼儿大面积皮肤血管瘤患儿按随机数字表法分为2组,进行前瞻性研究:观察组18例,采用90Sr-90Y敷贴治疗同时给予普萘洛尔口服;对照组21例,单纯采用90Sr-90Y敷贴治疗.对比观察2组患儿疗效,并对相关数据行秩和检验及x2检验.结果 观察组治愈率为44.4％ (8/18),好转率为55.6％ (10/18),有效率为100.0％ (18/18),加重率为0(0/18);对照组治愈率为14.3％ (3/21),好转率为52.4％(11/21),有效率为66.7％(14/21),加重率为19.0％ (4/21).观察组疗效优于对照组(Z=-2.861,P＜0.05);观察组1周内见效率为72.2％(13/18),高于对照组(14.3％,3/21;x2=13.447,P＜O.05);观察组不良反应发生率为66.7％(12/18),高于对照组(19.0％,4/21;x2=9.084,P＜0.05).结论 普萘洛尔联合90Sr-90Y治疗婴幼儿大面积皮肤血管瘤具有较好的临床疗效,但必须密切监测不良反应的发生.     

纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的实验研究

目的 探讨纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的能力及其作用机制.方法 6月龄雄性SD大鼠24只,体重(545±22)g.在双侧股骨髁部使用慢速钻钻取直径2 mm贯通双侧皮质的冠状轴洞性骨缺损.以纤维多孔钛微球复合纳米锶磷灰石填充左侧骨缺损,以单纯纤维多孔钛微球填充右侧.术后1、2、4、8周分别处死6只大鼠行X线、组织学及骨组织形态计量学观察,并进行比较分析.结果 影像学结果表明,两侧骨缺损修复效果均良好.组织学显示,纤维多孔钛微球允许骨长入,且左侧微球内新生骨多于右侧.骨组织形态计量学观察显示,随时间延长,左侧新生骨量逐渐增多,2、4、8周新生骨量存在差异,而4、8周时左侧与右侧的新生骨量比较,差异有统计学意义.结论 纤维多孔钛微球具有良好的生物相容性及骨传导性,可作为骨缺损修复的支架材料;纳米锶磷灰石可增强纤维多孔钛微球修复骨缺损的能力.     

Strontium ranelate improves bone strength in ovariectomized rat by positively influencing bone resistance determinants

Summary  Treatment of adult ovariectomized (OVX) rats with strontium ranelate prevented vertebral biomechanics degradation as a result of the prevention of bone loss and micro-architecture deterioration associated to an effect on intrinsic bone material quality. Strontium ranelate influenced the determinants of bone strength by prevention of ovariectomy-induced changes which contribute to explain strontium ranelate antifracture efficacy. Introduction  Strontium ranelate effects on the determinants of bone strength in OVX rats were evaluated. Methods  Adult female Sprague–Dawley rats were OVX, then treated daily for 52 weeks with 125, 250, or 625 mg strontium ranelate/kg. Bone strength, mass, micro-architecture, turnover, and intrinsic quality were assessed. Results  Strontium ranelate prevented ovariectomy-induced deterioration in mechanical properties with energy necessary for fracture completely maintained vs. SHAM at 625 mg/kg/day, which corresponds to the clinical dose. This was related to a dose-dependent effect on bone volume, higher trabeculae number, and lower trabecular separation in strontium ranelate vs. OVX. Load and energy required to induce lamella deformation were higher with strontium ranelate than in OVX and in SHAM, indicating that the bone formed with strontium ranelate is able to withstand greater damage before fracture. Bone formation was maintained high or even increased in strontium ranelate as shown by mineralizing surfaces and alkaline phosphatase while strontium ranelate led to reductions in deoxypyridinoline. Conclusion  Strontium ranelate administered at 625 mg/kg/day for 52 weeks prevented OVX-induced biomechanical properties deterioration by influencing the determinants of bone strength: it prevented bone loss and micro-architecture degradation in association with an effect on intrinsic bone quality. These beneficial effects on bone contribute to explain strontium ranelate antifracture efficacy.     

Strontium ranelate does not stimulate bone formation in ovariectomized rats

INTRODUCTION: Strontium ranelate (SrR) is suggested to function as a dual-acting agent in the treatment of postmenopausal osteoporosis with anti-resorptive and anabolic skeletal benefits. We evaluated the effects of SrR on the skeleton in ovariectomized (OVX) rats and evaluated the influence of dietary calcium. METHODS: Three-month old virgin female rats underwent ovariectomy (OVX, n = 50) or SHAM surgery (SHAM, n = 10). Four weeks post-surgery, rats were treated daily by oral gavage with distilled water (10 ml/kg/day) or SrR (25 or 150 mg/kg/day) for 90 days. Separate groups of animals for each dose of SrR were fed a low (0.1%) or normal (1.19%) calcium (Ca) diet. Static and dynamic histomorphometry, DXA, mu-CT, mechanical testing, and serum and skeletal concentrations of strontium were assessed. RESULTS: SrR at doses of 25 and 150 mg/kg/day did not increase bone formation on trabecular or periosteal bone surfaces, and failed to inhibit bone resorption of trabecular bone regardless of Ca intake. There were no improvements in bone mass, volume or strength with either dose of SrR given normal Ca. CONCLUSION: These findings demonstrate that SrR at dosages of 25 and 150 mg/kg/day did not stimulate an anabolic bone response, and failed to improve the bone biomechanical properties of OVX rats.     

89Sr Therapy: Strontium plasma clearance in disseminated prostatic carcinoma

Strontium plasma clearance is an important factor determining the absorbed dose to metastases and bone marrow in patients receiving ⁸⁹Sr radionuclide therapy for metastatic bone disease. Amongst male patients with disseminated prostatic carcinoma, the renal component of strontium clearance is frequently greatly reduced compared with values reported for healthy middle aged men. We report a study of renal and gut strontium plasma clearance, renal function, calcium urinary excretion, parathyroid function and extent of skeletal osteoblastic metastatic disease in patients referred for radiostrontium therapy for metastasised prostatic malignancy. The wide variation in net strontium clearance was principally due to variation in the renal component. Low values of strontium renal clearance were found to correlate with the elevation of serum PTH and nephrogenous cyclic AMP, which in turn correlated with extent of skeletal metastatic disease. This suggests that the osteosclerotic metastases characteristic of prostatic carcinoma induce secondary hyperparathyroidism due to the high avidity of the skeleton for calcium. The resulting reduction in strontium excretion may be beneficial to the objectives of radiostrontium therapy.     

Uptake of47Ca and85Sr in the tibia and the femur in rats

The ratio between the uptake of⁸⁵Sr and⁴⁷Ca was measured in the tibiae and femora of rats 24 h after injection of the tracers. There was a significant difference between shafts and ends but not between the different bones; in healed tibial fractures, 7 weeks old, the ratio was identical to that of normal tibial shafts. The findings were interpreted to be related to qualitative rather than quantitative differences in mineralization between cortical and trabecular bone.

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Zusammenfassung Das Verhältnis zwischen der Abnahme von⁸⁵Sr und⁴⁷Ca wurde von der Tibia und Femur 24 Std nach der Injektion bei Ratten gemessen. Man fand einen signifikanten Unterschied zwischen den Schaften und den Enden, aber nicht zwischen den verschiedenen Knochen. In 7 Wochen alten, verheilten Tibiafrakturen war das Verhältnis genau so wie in einem normalen Tibiaschaft. Das Resultat berechtigte zu der Annahme, daß der Unterschied in dem Mineralisierungsprozeß zwischen corticalen und spongiösen Knochen wahrscheinlich qualitativ und nicht quantitativ ist.

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Résumé La relation entre la résorption du⁸⁵Sr et du⁴⁷Ca était mesuré au tibia et fémur des rats 24 heures après l'injection. La différence entre les manches et les bouts était, remarquable, mais pas entre les os séparates; en les tibiae, qui etait fracturé depuis 7 semaines et guéries, la relation etait identique à celui des tibiae manches normals. On a présumé que la découverte était due à des différences qualitatives plustot que quantitatives entre les procédé de minéralisation dans l'os cortical et trabeculair.

     

钚与锶复合作用致大鼠骨肉瘤的相加效应

研究结果表明,两种核素复合作用致大鼠骨肉瘤的效应明显大于单一核素的作用。按照等效应图分析方法表明,复合作用组的两个点皆落在相加区域内,提示钚与锶对大鼠的复合作用基本上是属于相加效应。     

The incorporation and removal of large amounts of strontium by physiologic mechanisms in mineralized tissues of the rat

The incorporation and removal of strontium from the bones and teeth of rats under conditions of low and high dietary levels of strontium were investigated. In an otherwise satisfactory diet, an amount of strontium equimolar to that of calcium seriously debilitates the young growing rat and may culminate in death. The pathology due to strontium was found by the methods used only in the hard tissues. The excess osteoid formed originally in the presence of large amounts of dietary strontium is removed during a subsequent period of feeding on a low strontium regimen. Strontium removal from participation in physiologic processes appears to be of two types, (a) excretion from the body, and (b) incarceration within the hard tissues. The translocation of strontium to the incisor teeth has been demonstrated. Differences of sodium and potassium contents of the hemi-mandibulae of the experimental and control groups were also observed.This investigation was performed in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the University of Minnesota (A.R.J.) and was supported by Grant DE 01850 of the National Institutes of Health.