沉默LIMK1抑制人结肠癌SW480细胞上皮-间质转化

目的:研究沉默LIMK1对人结肠癌SW480细胞上皮-间质转化（epithelial-mesenchymal transformation,EMT）的影响。方法:RT-PCR、Western blot、免疫荧光与免疫组化检测沉默LIMK1对EMT相关分子表达的影响。裸鼠实验检测沉默LIMK1对移植瘤生长的影响。结果:RT-PCR检测显示,沉默LIMK1可下调Vimentin和上调E-cadherin mRNA表达(P<0.05)。Western blot显示,沉默LIMK1可明显下调SW480细胞Snail及Vimentin蛋白表达和上调E-cadherin表达(P<0.05)。免疫荧光显示,LIMK1沉默细胞Snail与Vimentin阳性信号较对照组明显减弱,而E-cadherin阳性信号显著增强。裸鼠实验结果显示,沉默组肿瘤生长速度较SW480组明显减慢(P<0.05)。沉默组移植瘤瘤重（0.16±0.079）g较SW480组（0.86±0.165）g明显减少,瘤重抑制率为97.7%(P<0.05)。LIMK1沉默组移植瘤较对照组的Ki-67、Vimentin、Snail与CD34阳性表达明显减弱,而E-cadherin表达明显增加。结论:沉默LIMK1可体外抑制人结肠癌SW480细胞EMT。     

上海市广州管圆线虫疫源地调查

[目的]了解上海市广州管圆线虫疫源地的分布及感染情况,为预防控制广州管圆线虫病提供科学依据。[方法]随机抽取南汇区新场镇和青浦区金泽镇为调查点。在调查点野外、自由市场、餐饮场所对广州管圆线虫的中间宿主、转续宿主和保虫宿主感染情况进行调查。[结果]调查点发现广州管圆线虫中间宿主螺类2种(铜锈环棱螺、中国圆田螺)、蛞蝓2种(双线嗜黏液蛞蝓、黄蛞蝓)和蜗牛1种(中华灰尖巴蜗牛),同时查到保虫宿主(鼠)和转续宿主(淡水鱼、河虾、青蛙、河蟹,蟾蜍)。经检测均无广州管圆线虫感染。[结论]本市目前尚无广州管圆线虫疫源地存在,但具备了该病流行的基本条件,一旦有该虫传入可引起传播,应加强健康教育。     

湖沼垸内型血吸虫病疫区渠道硬化消灭钉螺的研究

目的 为彻底消灭湖沼垸内钉螺,为控制血吸虫病疫情提供更为有效的灭螺方法和科学依据.方法 采用水泥管道及砖砌两种方法硬化渠道.结果 硬化24条有螺渠道,钉螺面积22.65万m2,灭光钉螺渠道16条,钉螺面积17.81万m2,分别占66.67%、78.63%.水泥管道硬化11条,水泥砖砌硬化13条,水泥管道硬化灭光钉螺渠道9条.水泥砖砌硬化灭光钉螺渠道7条,分别占81.82%、53.85%,灭光钉螺面积分别占 97.88%、55.66%.结论 硬化有螺渠道是一种环保型的有效灭螺方法,水泥管道硬化灭螺效果优于水泥砖砌硬化灭螺.     

Sex steroid receptor evolution and signalling in aquatic invertebrates

In vertebrate reproductive endocrinology sex steroids play a pivotal role via binding to receptors. However, information on the origin and relevance of sex steroids in invertebrates is limited. This review highlights current literature on steroid receptors in aquatic invertebrates and reports on some new findings. It has been shown that invertebrates of the deuterostome clade, such as Acrania and Echinodermata, respond to estrogens and androgens and, at least in Branchiostoma, an estrogen receptor has been cloned. Within the protostomes, most findings are related to aquatic molluscs. Sex steroid receptor-like proteins are abundant in gastropods, bivalves and cephalopods and also sex hormone signalling shows partial similarity to the deuterostomes. In ecdysozoans, however, the impact of sex steroids is still a matter of debate even though there is evidence on the presence of estrogen receptor-like proteins in Crustacea and on physiological effects of estrogens in both Nematoda and Crustacea. Recent findings suggest the presence of an estrogen receptor α-like protein of unclear physiological role in Gammarus fossarum (Crustacea). Binding studies revealed the crustacean Hyalella azteca to possess specific binding sites only for androgens but not for estrogens suggesting a possible limitation to functional androgen receptors in this species. Further studies have to be conducted to shed more light into the discussion about the controversy about sex steroid receptors in invertebrates.     

Snail与E-cadherin在上皮性肿瘤中的表达及其临床意义

目的:探讨Snail与E-cadherin蛋白表达在上皮性肿瘤中的临床意义。方法:应用免疫组织化学SP法,研究150例人肝癌、乳腺癌、宫颈癌、子宫内膜癌与卵巢癌临床标本及30例相应正常组织中Snail与E-cadherin蛋白的表达及定位。结果:在上述5种组织中,Snail主要表达于癌组织细胞核、细胞质,相应正常组织往往缺如,且在肝癌、乳腺癌中Snail表达与肿瘤转移有一定的相关性,并与E-cadherin表达呈负相关。E-cadherin定位于细胞膜,在肝、乳腺的正常组织中E-cadherin强表,相应癌组织中其表达往往下降或缺如;而在宫颈癌、内膜癌与卵巢癌中,E-cadherin表达与癌变无明显相关性。结论:Snail、E-cadherin的表达在肝癌、乳腺癌中具有重要意义,Snail有可能作为肝癌、乳腺癌肿瘤转移的生物学标志。     

pEGFP-C1-STAT3真核表达载体的构建及其在SGC7901细胞株中对Snail表达的影响

目的研究信号转导与转录激活因子3（STAT3）基因转染人胃癌细胞株SGC7901后,对锌指转录因子Snail1（Snail）及切除修复交叉互补基因1（excision repair cross complementation group 1,ERCC1）表达的影响。方法利用基因重组技术构建重组pEGFP-C1-STAT3真核表达载体;脂质体介导转染技术转染胃癌细胞株SGC7901,荧光显微镜、Western blot 检测转染后SGC7901细胞株中STAT3、pSTAT3、Snail及ERCC1的表达,流式细胞仪检测转染后顺铂处理下细胞凋亡率。结果重组质粒经Hind Ⅲ、SacⅡ双酶切测序与STAT3基因序列一致;利用脂质体介导将pEGFP-C1-STAT3重组质粒转染SGC7901细胞株,获得了STAT3基因表达,Western blot 检测pSTAT3、Snail、ERCC1表达增高,具有统计学意义,流式细胞术分析显示转染重组质粒pEGFP-C1-STAT3后,细胞在顺铂作用下的早期凋亡率减低。结论成功构建STAT3重组质粒并转入中分化胃腺癌细胞株SGC7901后,细胞Snail及ERCC1显著增高,细胞对顺铂敏感度下降。