一测多评法测定竹节参中7种皂苷类成分的含量

目的建立一测多评法同时测定竹节参中7种皂苷类成分的含量,并验证此方法在竹节参中应用的可行性与适用性。方法采用HPLC法,以竹节参皂苷Ⅴ为内参物,建立人参皂苷Rg1、Re、Rb1,假人参皂苷RT1,竹节参皂苷Ⅳ、Ⅳa的相对校正因子(fs/i),并利用fs/i计算竹节参样品中成分的含量,同时用外标法测定各成分的含量,比较2种方法的差异。结果人参皂苷Rg1、Re、Rb1,假人参皂苷RT1,竹节参皂苷Ⅳ和Ⅳa的fs/i分别为1.286 7、1.432 7、0.966 6、0.962 4、1.207 2、0.938 4。采用一测多评法与外标法得到的10批竹节参样品各成分含量无显著性差异。结论建立的一测多评法准确性高,可用于竹节参皂苷类成分的定量分析和质量评价。     

复合PCR鉴别葡萄球菌及其多重耐药基因

目的　建立既可鉴别金黄色葡萄球菌又可同时检测其耐药基因的分子诊断方法。方法　对应于femB、mecA、ileS基因的 3对引物与快速提取的单菌落模板DNA进行单管同步扩增 ,电泳观察PCR片段 ;mecA、ileS耐药基因扩增结果分别与苯唑西林、莫匹罗星药敏试验对比 ,分析菌株的耐药性。结果　检测femB基因可快速特异性地筛选出金黄色葡萄球菌 ,mecA基因的检出与常规药敏试验鉴定耐甲氧西林葡萄球菌 (MRS)的结果基本一致 ,而拥有ileS基因的全部葡萄球菌分离株对莫匹罗星耐药。结论　复合PCR可快速敏感地从葡萄球菌中区分金黄色葡萄菌 ,并同时检出MRSA和耐莫匹罗星的多重耐药菌株。     

淋病奈瑟菌隐蔽性质粒的克隆及PCR的建立与应用

用分生物学方法自淋病奈瑟菌标准分离株中克隆４．２ｋｂ的隐蔽性质粒，并根据该质粒ＤＮＡ序列，设计引物，建立淋病奈瑟菌ＰＣＲ检测方法。该方法标处理简单、反应程序优化，可在２．５小时内完成一次检测。     

注射狂犬疫苗饮酒引起过敏反应一例报告

本文建立了一个用于检测军团菌的多聚酶链反应方法。扩增参考菌株的染色体DNA(L.pneumophital-14、L.micdadei、L.dumoffii Llongbeachae、L.Jordanis、L.bozemanii)，均可检出375bp的16SrRNA基因片段。对于已经监定的5株国内分离菌株染色体DNA进行扩增，获得了相同的结果，地高辛标记16SrRNA基因探针与扩增产物杂交，结果为阳性。而扩增14株非军团菌均为阴性。采用煮沸法制备细菌染色体DNA,PCR法检测环境水军团菌敏感性为280cfu/ml水，检查临床标本军团菌为560cfu/ml支气管灌洗液。上述结果表明该法敏感、快速、简便，具有较好的特异性。     

实时超声监测药物诱导排卵的临床应用

药物诱导排卵和自然排卵周期中卵泡生长发育的超声表现相似，但仍在某些重要差异。本文应用超声对４１例药物诱导排卵的妇女进行了动态监测，认为超声不但能准确地判断卵泡的生长发育，且能指导针对性地定ｈｃＧ的给药，促进成熟和排卵，减少和避免因药物诱导排卵而造成的并发症，尤其是ＯＨＳＳ的发生。     

CDC42在HBx介导肝细胞恶性转化中作用的定量蛋白质组学研究

目的寻找在乙肝病毒 X 蛋白（HBx）诱发肝细胞恶性转化过程中 CDC42信号通路发挥作用的介导因子。方法利用基于质量亏损的准等重二甲基标记（pIDL）的定量蛋白质组学方法,检测 CDC42基因敲除前后HBx 转基因细胞蛋白质组的差异。筛选差异蛋白并对差异蛋白进行基因本体（gene ontology,GO）功能分析。结果与结论共定量到3409个蛋白,筛选出220个差异蛋白,通过 GO 分析发现与细胞骨架组织相关的 palladin、成蛋白样亚家族1（formin-like 1,FMNL1）和角蛋白-19均发生显著下调。该研究为 CDC42在 HBx 介导 Huh7细胞恶性转化的机制研究提供了候选基因和蛋白。     

Variants in the UBR1 gene are not associated with chronic pancreatitis in Japan

Background/objectivesThe UBR1 gene encodes the enzyme ubiquitin-protein ligase E3 component n-recognin 1. Loss-of-function mutations in the UBR1 gene cause Johanson-Blizzard syndrome, which involves pancreatic exocrine insufficiency. No previous studies have examined an association of UBR1 variants with pancreatitis, in part due to the large size of the gene. This study aimed to clarify whether UBR1 variants are associated with chronic pancreatitis (CP) by the application of targeted next generation sequencing.MethodsExon sequences of the UBR1 gene from 389 Japanese patients with CP (188 idiopathic, 172 alcoholic, 20 hereditary, 9 familial) were captured by the HaloPlex target enrichment technology and subjected to next generation sequencing.ResultsNinety nine point two % of the coding regions of the UBR1 gene could be sequenced by ≥ 20 reads with a mean read depth of 595 and a median depth of 399. Fifteen non-synonymous variants including three novel ones [c.4514T > C (p.I1505T), c.4828C > G (p.H1610D) and c.4856A > T (p.D1619V)] and two synonymous variants were identified in the exonic regions. The frequency of any non-synonymous or synonymous variants was not different between the patients with CP and controls.ConclusionsVariants in the UBR1 gene were not associated with CP in Japan.     

Presence of cytomegalovirus in the perilymphatic fluid of patients with profound sensorineural hearing loss caused by congenital cytomegalovirus infection

Conclusion: Not all patients diagnosed with congenital infection using umbilical cord assay were found to be positive for CMV-DNA by perilymphatic fluid assay. In addition, a CMV-DNA-positive result was observed in one patient who had not been diagnosed with congenital infection. Sampling of perilymphatic fluid from a large population of patients with congenital SNHL caused by congenital CMV infection or of unknown etiology is required to determine the prevalence of CMV-related profound HL. Objectives: Sensorineural hearing loss (SNHL) is one of the most frequent manifestations in patients with congenital cytomegalovirus (CMV) infection. Using dried umbilical cord, a PCR-based assay was recently developed for the retrospective detection of congenital CMV infection. This study analyzed the presence of CMV in the perilymphatic fluid and evaluated differences in the effect of cochlear implantation between CMV-positive and -negative groups. Method: Perilymphatic fluid was collected from each patient at the time of cochlear implantation and analyzed for the presence of CMV using a PCR method. Results: The perilymphatic fluid in two of the five patients suffering from congenital CMV infection and in one of the 17 patients without congenital CMV infection was found to be positive for CMV.     

Labour experience and postpartum stress and depression: a quantitative and qualitative examination

Objectives: The purpose of this study was to examine the relationship between qualitatively and quantitatively assessed birth experiences and rates of post-birth distress and depressive symptoms three to four weeks postpartum. Both the rates of post-birth distress and depressive symptoms represented risk factors for subsequent mental health problems in the later postpartum period. Background: Childbirth is accompanied by various stress factors. However, little is known about the relationship between stressors occurring during birth (intrapartum) and the ways women cope with them and women’s development of depressive symptoms or acute stress reactions postpartum. Methods: One hundred and twenty-seven women from two longitudinal studies were interviewed 48–96 h after childbirth. Thirty birth interviews from both samples were additionally examined for qualitative themes related to women’s reported experience in connection with mental health adaptation (i.e. without symptoms (n = 10), symptoms of depression (n = 10) and acute stress reactions (n = 10)) at three to four weeks postpartum. Results: Women with depressive symptoms reported less intimate and helpful contact with their partners and baby during labour compared with women without symptoms or with acute stress reactions. Women with acute stress reactions had less confidence in themselves, and reported disorientation during the birth process, compared with women without symptoms or with depressive symptoms. Conclusions: Recognition of how women cope with intrapartum factors during labour could help to identify psychological distress shortly after delivery, and inform the introduction of timely and appropriate psychological support for affected women.