115例老年人胃息肉临床及病理观察

目的 研究老年人胃息肉的临床、病理特点及演变规律。方法 对３６４２名老年人进行胃镜检查,检出胃息肉患者１１５例,随访２～１０年,平均随访６．５年,对所发现的胃息肉根据情况均进行内镜下切除（钳除或电切）,送病理检查。结果 对３６４２例老年人行胃镜检查,发现胃息肉患者１１５例,检出率为３．２％;其中炎性、增生性、腺瘤性息肉分别占６０．８％、３３．６％、５．６％;息肉部位分别为贲门１３．３％、胃底２０．     

CD45RO CD20及总IgE在声带息肉组织中表达的研究

目的 :从免疫病理学角度探讨细胞免疫、体液免疫和局部变态反应在声带息肉发病中的作用。方法 :对 30例声带息肉组织 (息肉组 )和 10例正常声带组织 (对照组 )采用免疫组织化学方法观察两组CD4 5RO、CD2 0及IgE的表达情况并检测其阳性细胞数。 结果 :息肉组CD4 5RO、CD2 0和IgE阳性细胞数显著高于对照组 (P <0 .0 1) ;声带息肉组织中CD4 5RO、CD2 0和IgE的表达之间无相关性 ;男、女患者间的表达差异亦无统计学意义 (P >0 .0 5 )。 结论 :由T淋巴细胞介导的细胞免疫和B淋巴细胞介导的体液免疫在声带息肉中免疫应答活跃 ;声带黏膜局部变态反应参与了声带息肉的发病 ;在声带息肉的发病机制中免疫病理改变起着重要的作用。     

输尿管结石并发输尿管息肉的治疗

目的探讨输尿管镜气压弹道碎石术并配以自制电刀治疗输尿管结石并发输尿管息肉的方法及疗效。方法对78例输尿管结石并发输尿管息肉的采用输尿管镜气压弹道碎石术并配以自制电刀治疗。结果78例均获成功,患者术后症状全部消失。结论输尿管镜气压弹道碎石术并配以自制电刀治疗输尿管结石合并输尿管息肉,具有安全可靠、损伤少,术后恢复快等优点,是微创治疗的有效方法之一。     

宫腔镜下子宫内膜息肉切除术联合地屈孕酮治疗子宫内膜息肉60例

目的 分析宫腔镜下子宫内膜息肉切除术(TCRP)联合地屈孕酮(达芙通)治疗子宫内膜息肉的疗效及对预后的影响.方法 选取2015年1月至2018年11月皖北煤电集团总医院接收的子宫内膜息肉病人120例,据随机数表法分为两组,每组60例,对照组单行TCRP治疗,观察组行TCRP+地屈孕酮治疗,观察两组TCRP术后第12、1...     

Hysteroscopic Endometrial Polypectomy: Clinical and Economic Data in Decision Making

Study Objective

To compare the costs of hysteroscopic polypectomy using mechanical and electrosurgical systems in the hospital operating room and an office-based setting.

Efficacy and safety of SIC-8000 (Eleview®) for submucosal injection for endoscopic mucosal resection and endoscopic submucosal dissection in an in vivo porcine model

BackgroundSubmucosal injection is generally required for both endoscopic-mucosal resection (EMR) and submucosal dissection (ESD). SIC-8000 (Eleview™) is a new FDA 510(k) cleared and CE marked liquid composition for submucosal injection, containing a biocompatible polymer as a cushioning agent.AimsThe aim of this randomized study was to compare Eleview with saline solution when performing upper- and lower-GI EMR/ESD in a porcine model.MethodsEMR/ESD procedures were performed in a total of 10 white domestic pigs comparing Eleview vs. NaCl 0.9% containing methylene blue at 0.001% (control solution) as submucosal solution to be injected. Animals were randomized between EMR (22 procedures) and ESD (22 procedures) and between upper- (8 animals) and lower-(2 animals) GI procedures. All procedures were performed aiming for a resection size of 1 cm × 1 cm for EMR and 2 cm × 2 cm for ESD. Volume of injected fluid, number of injections, time of resection, technical success, en-bloc resection, and adverse events were collected. Endoscopic surveillance was conducted each week for 4 weeks after the EMR/ESD, including biopsies on the margins of resection sites at week-1 of follow-up. After four weeks, pigs were sacrificed and necropsy performed.ResultsOverall, 22 EMR and 22 ESD were performed in 10 live pigs. The average total volume needed for EMR and ESD was statistically significantly lower with Eleview as compared with control solution for both of the techniques (EMR: 5.6 ± 3.4 ml vs. 11.5 ± 6.7 ml; p = 0.02; ESD: 19.0 ± 10.0 ml vs. 36.3 ± 16.9 ml; p = 0.02). The rate of adverse events was statistically significantly lower in the Eleview than in the control group (2/26, 7.7% vs. 6/18, 33.3%; p = 0.03), but most of them were minor. No difference between Eleview and control solution was found in the rate of technical success (EMR: 11/13, 84.6% vs. 7/9, 77.8%; p = 0.7; ESD: 13/13, 100% vs. 9/9, 100%; p = 1), en-bloc resection (EMR: 6/13, 46.2% vs. NaCl: 5/9, 55.6%; p = 0.06; ESD: 12/13, 92.3% vs. 8/9, 88.9%; p = 0.8), and time of resection (EMR: 9.1 ± 7.75 min vs. 9.4 ± 9.6 min; p = NS; ESD: 38.4 ± 17.2 min vs. 40.2 ± 19.1 min; p = NS). At endoscopic follow up and necropsy, no difference in the process of healing of post-endoscopic ulcer and in the histological inflammation at the site of resection was shown between the two groups.ConclusionsThe new submucosal injection composition Eleview appeared to be an effective alternative to saline solution for submucosal injection for EMR/ESD, resulting in a substantial reduction of the volume administered to achieve an adequate lifting. No signs of adverse local or distant tissue reaction to Eleview were found at long-term follow up.     

Fecal Genetic Mutations and Human DNA in Colorectal Cancer and Polyps Patients

Background: Colorectal cancer (CRC) is one of the most frequent cancers. Genetic mutations in CRC already described can be detected in feces. Microarray methods in feces can represent a new diagnostic tool for CRC and significant improvement at public health. Aim: to analyze stool DNA by human DNA quantify and microarray methods as alternatives to CRC screening. Method: Three methods were analyzed in stool samples: Human DNA Quantify, RanplexCRC and KRAS/BRAF/PIK3CA (KBP) Arrays. Results: KBP array mutations were presented in 60.7% of CRC patients and RanplexCRC Array mutations in 61.1% of CRC patients. Sensitivity and specificity for human DNA quantification was 66% and 82% respectively. Fecal KBP Array had 35% sensitivity and 96% specificity and RanplexCRC Array method had 78% sensitivity and 100% specificity. Conclusion: Microarray methods showed promise as potential biomarkers for CRC screening; however, these methods had to be optimized to improve accuracy and applicability by clinical routine.     

胆囊息肉样病变的自然病程及相关危险因素分析

目的调查分析某地区胆囊息肉样病变（PLG）的自然病程及相关危险因素对其病程变化的影响。 方法统计西南医科大学附属医院2010年3月至2016年1月经超声诊断为PLG并在肝胆外科门诊长期随访的510例患者临床资料,分析PLG直径增长情况以及危险因素。 结果510例中单发息肉293例（57.5%）,随访15（2~58）个月,300例（58.8%）息肉直径增大2（1~10）mm,年平均增长速度为0.4 mm;196例（38.4%）直径无变化;8例直径减小;6例消失。单因素分析及多因素Logistic回归分析显示,饮酒和睡眠质量差是PLG直径增大的危险因素（P<0.01）,饮酒者的PLG直径增大的风险是非饮酒者的2.615倍,睡眠质量差者的息肉增大风险是睡眠质量正常者的2.282倍。 结论泸州地区PLG患者的增长速度缓慢,饮酒、睡眠质量差可能是促进胆囊息肉增长的危险因素。     

鼻息肉组织中白细胞介素5mRNA表达状况的半定量分析

目的 观察鼻息肉组织中白细胞介素5(Interleukin-5,IL-5)mRNA的表达水平。方法 采用逆转录PCR(RT-PCR)法,半定量检测鼻息肉(11例)和正常中鼻甲(8例)组织中IL-5 mRNA表达水平。结果 鼻息肉中IL-5 mRNA表达状况的半定量分析显示,11例鼻息肉标本RT-PCR产物中均可见IL-5和β-actin的扩增条带,IL-5/β-actin光密度比值0.711±0.112,IL-5 mRNA阳性表达率为100％。而8例正常中鼻甲组织的扩增产物中只有1例显示两条条带,余者均只有β-actin条带,IL-5 mRNA阳性表达率为12.5％。结论 鼻息肉中IL-5 mRNA阳性表达水平高于正常鼻粘膜组织,提示鼻息肉组织中IL-5mRNA的表达水平在鼻息肉的发病机制中发挥作用。     

Unilateral auditory neuropathy spectrum disorder

A polymerase chain reaction (PCR)-based genotyping of the penicillin-binding protein (PBP) genes pbp1a , pbp2x and pbp2b was used to characterize Streptococcus pneumoniae isolated from the nasopharynx of children with acute otitis media (AOM). Mutations were observed in pbp1a , pbp2x and pbp2b genes in 36.5% of the strains. Decreased susceptibility to &;#103 -lactam antibiotics was closely associated with the frequency of mutations in the three PBP genes. Of penicillin-intermediately-resistant S. pneumoniae strains, 54.5% appeared to be genetically similar to penicillin-resistant S. pneumoniae strains. Of penicillin-susceptible S. pneumoniae strains, 33.3% had mutations in the pbp2x gene and showed relatively high MICs to cephalosporins. Strains with mutations in the three PBP genes were often isolated from children &;#104 2 years old. Evaluation of mutations in PBP genes using PCR will prove useful for studying the epidemiology of antibiotic resistance.