Analysis of HLA-G gene polymorphism and protein expression in invasive breast ductal carcinoma

The human leukocyte antigen G (HLA-G) is a non-classical HLA class I molecule predominantly expressed in trophoblastic placental cells to protect the fetus during pregnancy. However, evidence has shown that this molecule may be implicated in the immune escape mechanism of tumor cells. Thus, the aim of this study was to evaluate the frequency of 14-bp insertion/deletion HLA-G polymorphism, as well as the expression of this molecule in patients with invasive breast ductal carcinoma (IDC). A significant association between the expression of HLA-G and the presence of metastasis in lymph nodes (p = 0.01) was observed and the expression of HLA-G was significantly higher in patients with shorter survival time (p = 0.03). The analysis suggests that the polymorphism observed in patients with IDC may be inducing a higher expression of the HLA-G molecule, which may possibly contribute to shorter survival time and a worse clinical prognosis for such patients.     

Significance of uroplakin III expression in recurrence of solitary muscle non-invasive bladder cancer

Numerous immunohistochemical biomarkers for patients with urothelial bladder cancer have been identified in order to predict their biological behavior. The aim of this present study was to examine the uroplakin III (UPIII) expression in homogenous group of non-muscle invasive bladder cancer and to correlate its value with clinico-pathological characteristics of patients and moreover with COX-2 expression and tumor infiltrating lymphocytes (TILs).     

Gastric carcinoma with an invasive micropapillary carcinoma component showing HER2 gene amplification and CD10 expression: A case report and review of the literature

An 81‐year‐old man was referred to our hospital because gastric cancer was detected on screening esophagogastroduodenoscopy. Surgical resection of the tumor was performed. It was 25 × 20 mm in size and swollen lymph nodes were present nearby. Lymphadenectomy was also performed. Histopathologically, the tumor reached the proper muscle layer via venous invasion. There were three components that constituted the tumor, that is, 40% of mass was invasive micropapillary carcinoma (IMPC), 40% was papillary adenocarcinoma and 20% tubular adenocarcinoma. Vascular invasion was prominent. Immunohistochemistry revealed that the tumor showed an entirely intestinal mucin phenotype, being positive only for CD10 and negative for MUC2, MUC5AC, and MUC6. HER2 staining score ranged from 2+ to 3+, depending on the components described above. HER2 gene amplification was present in all the components according to dual‐color in situ hybridization. The metastatic lymph nodes were similar to the primary site in morphology and immunohistochemistry, but HER2 amplification was higher in the lymph nodes. The IMPC component with HER2 amplification is rarely seen and its positivity for CD10 is an unexpected finding for gastric IMPC. Hence, this is a highly unusual case judging by the literature; further studies are needed to clarify the nature of gastric IMPC.     

不同手术方法治疗腋臭临床疗效分析

目的观察不同手术术式治疗腋臭的临床疗效,探讨和寻求疗效最佳的手术方法。方法对我院2011年1月至2013年7月收治的200例腋臭患者的临床资料进行回顾性分析,观察不同手术方法的远期疗效。手术方法分为传统腋臭切除术、微创刮除术、射频笔触式电针烧灼术和改良式切刮术,对观察数据进行统计学分析。结果传统切除术80例,手术切口平均愈合时间为18.7 d,远期疗效治愈78例(97.5%),显效2例(2.5%)。微创刮除术60例中,切口平均愈合时间为8.6 d;远期疗效治愈3例(5.0%),显效8例(13.3%),好转16例(26.7%),弱效4例(6.7%),无效29例(48.3%)。射频笔触式电针烧灼术30例,显效5例(16.7%),好转8例(26.7%),无效17例(56.6%)。改良式切刮术30例,伤口平均愈合时间为9.8 d,远期疗效治愈28例(93.3%),显效2例(6.7%)。结论传统切除术疗效好,但是伤口瘢痕大,恢复期长;微创刮除术和射频笔触式电针烧灼术恢复快,但是复发率高;改良式刮除术吸收了传统和微创手术的优点,恢复快,疗效肯定,缺点是伤口瘢痕较大,但是总体疗效令人满意,可以作为治疗腋臭的首选手术方法。     

腹腔镜下经肛微创手术治疗直肠肿瘤的疗效分析

目的探讨腹腔镜器下完成经肛微创手术治疗直肠肿瘤的可行性及疗效。方法我院2012年6月至12月14例适合于局部切除的直肠良性肿瘤、类癌病例选择腹腔镜下经肛微创治疗。手术方法以肛管扩张器、手套联合切口保护套建立腹腔镜操作通路,使用腹腔镜/结肠镜作为照明和充气设备,超声刀完成直肠黏膜下切除或全层切除,全层切除创面采用可吸收线缝合。结果 14例患者手术过程顺利,无围手术期并发症。手术时间30~70 min,其中建立操作入路平均花费15 min。切除的肿瘤直径1.5~3.8 cm,大体观和病理检查证实均为R0切除。随访6~12个月无复发及远期并发症。结论使用腹腔镜器械设备完成经肛微创手术治疗直肠肿瘤安全有效,但该术式部分操作细节仍需改进。     

不同微创入路在跟骨关节内骨折治疗中应用

目的探讨纵行切口和跗骨窦微创入路在跟骨关节内骨折治疗中的临床疗效。方法回顾性分析2008年3月至2012年3月我院收治的67例纵行切口或跗骨窦微创入路术治疗并跟踪随访的跟骨关节内骨折患者的临床资料,按照手术类型分为2组:纵行切口微创入路组(ZW组)36例(37足),行纵行复位小切口入路治疗方案;跗骨窦微创入路组(FW组)31例(33足),行跗骨窦间隙入路治疗方案。根据X线片对比2组患者手术前后的Bohler角、Gissane角、并发症、愈合时间、跟骨内骨折术后AOFAS踝关节与足后评分。结果 ZW组愈合时间、Bohler角、Gissane角等指标均优于FW组,但组间比较无显著性差异(P0.05);ZW组切口感染并发症明显少于FW组,组间比较差异具有统计学意义(P0.05);评分结果显示ZW组得分为82.49分,FW组得分为86.53分,组间比较无统计学意义(P0.05)。结论 2种治疗手段虽无明显差异,但经纵行小切口复位微创入路治软组织损伤程度、术后愈合时间、手术并发症等临床效果更为满意。     

Outcomes of implants placed with three different flapless surgical procedures: A systematic review

The aim of this systematic review was to evaluate the outcomes of flapless surgery for implants placed using either free-hand or guided (with or without 3D navigation) surgical methods. Literature searches were conducted to collect information on survival rate, marginal bone loss, and complications of implants placed with such surgeries. Twenty-three clinical studies with a minimum of 1 year follow-up time were finally selected and reviewed. Free-hand flapless surgery demonstrated survival rates between 98.3% and 100% and mean marginal bone loss between 0.09 and 1.40 mm at 1–4 years after implant insertion. Flapless guided surgery without 3D navigation showed survival rates between 91% and 100% and mean marginal bone loss of 0.89 mm after an observation period of 2–10 years. The survival rates and mean marginal bone loss for implants placed with 3D guided flapless surgery were 89–100% and 0.55–2.6 mm, respectively, at 1–5 years after implant insertion. In 17 studies, surgical and technical complications such as bone perforation, fracture of the surgical guide, and fracture of the provisional prosthesis were reported. However, none of the identified methods has demonstrated advantages over the others. Further studies are needed to confirm the predictability and effectiveness of 3D navigation techniques.     

改良头皮冠状切口在颧骨复合体骨折中的应用

目的:探讨改良头皮冠状切口在各类颧骨复合体骨折坚固内固定术中的应用。方法:将87例颧骨复合体骨折病例分为改良组和传统组,分别应用改良冠状切口(42例)与传统切口(45例)进行复位和固定,对比分析改良冠状切口的优越性、可能并发症及预防措施。结果:改良组切口均Ⅰ期愈合,随访3⁶个月,面部瘢痕不明显,开口度恢复理想,咬合关系正常,术后秃发率及颞部凹陷率较低,无1例出现面神经功能障碍。传统组中,2例术后第5天出现创区感染,切口延期愈合,术后肿胀反应明显;4例患者术后3月时仍存在面神经功能障碍,术后秃发率及颞部凹陷率较高。结论:改良冠状切口较传统切口具有切口隐蔽、术野暴露好、复位精确、美观效果好、面神经损伤率低等优点,值得推广应用。