蝌蚪提取液诱导人早幼粒白血病细胞分化的实验研究

目的 :探讨蝌蝌提取液 (T871)对白血病细胞的诱导分化作用。方法 :利用细胞生长曲线、形态学观察、细胞免疫化学及原位mRNA杂交和完整细胞原位斑点印迹技术 ,观察了T871诱导HL 60细胞分化的作用和分化过程中癌基因c myc、c myb表达的变化。结果 :T871能抑制HL 60细胞的增殖 ,形态学表现为类似单核 /巨噬细胞 ,同时T871还能使HL 60细胞的NBT还原能力、ANAE活性显著增强 ,同时伴有癌基因c myc、c mybmRNA表达的下调。结论 :T871能诱导HL 60细胞向单核 /巨噬细胞方向分化 ,c myc、c myb癌基因可能参与了此过程     

某医科大学中老年教职工健康状况分析

对１００４名５０岁以上中老年教职工进行健康检查。结果：健康者仅占１１．２５％，而８８．７５％的中老年教职工至少患有１例次疾病，最多患有５例次疾病，总例次患病率为１８６．９５％，男女例次患病率分别为１８７．２４％和１８６．６２％，男女间无差异。前５位疾病的顺位是：心血管疾病、呼吸系疾病、脑血管疾病、消化系统病、泌尿生殖疾病。     

Expression of the cell surface antigen detected by the monoclonal antibody A7 in pancreatic carcinoma cell lines

In a previous study, we used a murine monoclonal antibody, A7, against human colon carcinoma as a drug-carrier to treat colorectal cancer.¹ In the present study, we found that MAb A7 also reacted immunohistochemically with 73% of human pancreatic carcinoma cell lines, with the A7 antigen mainly being detected on the cell surface. However, the A7 antigen was found in only 9% of the spent media of these human pancreatic carcinoma cell lines by ELISA. On the other hand, the positive incidence of CA19-9, POA, ferritin, CEA, DU-PAN-2 and SLX in those spent media was 100%, 64%, 64%, 55%, 55% and 36%, respectively. These results suggest that the A7 antigen may only rarely be shed into the sera of pancreatic cancer patients, in which case MAb A7 could be a suitable drug-carrier in targeting chemotherapy for pancreatic cancer patients.     

依托咪酯对豚鼠听诱发电位的影响

为探讨听诱发电位能否作为临床麻醉深度监测手段,采用非镇痛性麻醉药依托咪酯分次注入豚鼠腹腔,根据用药剂量模拟浅、中、深度麻醉,观察豚鼠听觉中潜伏期反应及听性脑干反应变化.结果显示,依托咪酯对听觉中潜伏期反应有明显抑制作用,且随麻醉深度加深而增强,但到一定深度后,出现“饱和”现象;中潜伏期反应谱峰明显向低频移动.依托咪酯对浅麻醉时听性脑干反应影响不显著,当麻醉加深时亦出现明显改变.提示中潜伏期反应及听性脑干反应结合使用,可以作为监测麻醉深度的客观电反应指标.     

强酸和强碱中和过程的PH控制

讨论了强酸、强碱中和过程ＰＨ控制方案。以０．９ｍｏｌ／Ｌ的ＮａｏＨ中和０．９ｍｏｌ／Ｌ的ＨＮＯ３，流量增量为０．１－０．３ｋｇ／ｍｉｎ，用模糊控制将ＰＨ控制在各要求的值上，ΔｐＨ≤０．２，获得满意的结果。     

氯化锂对K562白血病细胞增殖和凋亡的影响

目的 :研究氯化锂 (LiCl)在体外对K5 6 2白血病细胞增殖及凋亡的影响。方法 :采用液体培养试验 ,四唑盐 (MTT)比色试验 ,集落培养试验为指标观察LiCl对K5 6 2细胞增殖的影响 ,采用DNA片段凝胶电泳及细胞形态学改变为指标检测细胞凋亡。结果 :①不同浓度的LiCl(10～ 5 0mmol/L)对K5 6 2细胞具有抑制增殖的作用 ,这种增殖抑制作用呈剂量依赖关系。②在LiCl(30mmol/L)作用下 ,K5 6 2细胞形态学上可见凋亡细胞 ,且DNA琼脂糖凝胶电泳谱可见“梯形带”(DNAladder)。结论 :LiCl能抑制K5 6 2白血病细胞增殖和诱导其凋亡。     

Human T-Cell Leukemia Virus-1-positive Cell Line Established from a Patient with Small Cell Lung Cancer

A stable cell line, KHM-3S, was established from a patient with small cell lung cancer (SCLC), who had a high serum level of soluble interleukin 2 receptors (sIL2-R) and was seropositive for human T cell leukemia virus (HTLV)-l. KHM-3S cells were positive for IL2-R (Tac) and NKH-1, but negative for other lymphocytic markers such as OKT 11, OKT 4, OKT 8, T cell receptor (WT 31), B 1, and B 4. Moreover, the KHM-3S cells were negative for leukocyte common antigen and strongly positive for neuron-specific enolase (NSE). Secretion of sIL2-R and NSE by the KHM-3S line was detected by an enzyme-linked immunosorbent assay. Rearrangement of the T cell receptor gene and monoclonal HTLV-1 integration were found by Southern blot analysis of KHM-3S DNA. However, Northern blot analysis showed no T cell receptor mRNA. KHM-3S may be useful for studies on the role of HTLV-1 in carcinogenesis and IL2-R expression in SCLC.     

猕猴实验性脑动脉硬化扫描电镜观察

本实验表明,猕猴高脂造型22—28个月,其大脑中动脉(Middle Cerebral Artery:MCA)内皮发生水肿、细胞间隙变宽,局部破裂等改变,中膜平滑肌细胞增生、迁移。老年高脂组病损程度较重,且中央支也表现出类似改变。结果提示,高血脂可以引起脑动脉硬化性改变,且病损程度与年龄因素密切相关。     

生长抑素受体亚型在人鼻咽癌细胞株CNE2中表达

背景与目的：生长抑素受体（somatostatin receptor SSTR）在许多肿瘤组织均有表达，为生长抑素类似物如奥曲肽用于这些肿瘤的治疗提供了生物学基础，但对生长抑素受体在鼻咽癌中表达情况的研究甚少。本研究旨在了解生长抑素受体基因在鼻咽癌细胞中的表达情况，为生长抑素类似物用于鼻咽癌治疗的进一步研究提供试验基础。方法：采用RT—PCR法和SP免疫组化法联合检测体外培养的人鼻咽癌细胞株CNE2的SSTRs表达，并对PCR mRNA产物测序以鉴定结果。结果：RT—PCR提示人鼻咽癌细胞株CNE2分别表达生长抑素受体亚型SSTR1、SSTR2、SSTR4；免疫组化结果：人鼻咽癌细胞株CNE2表达SSTR1、SSTR2A为强阳性（〉60％），表达SSTR4为弱阳性、SSTR2介于两者之间、SSTR3和SSTR5则无表达。结论：我们的研究证实人鼻咽癌细胞株CNE2有多种生长抑素受体亚型基因表达，且以表达SSTR1、SSTR2较多。     

横窦和窦汇的浅表解剖学标志

目的为神经外科手术准确定位横窦的下缘和窦汇确定出一个可靠的浅表解剖学标志。方法对10例经10%甲醛溶液固定的尸头标本进行逐步解剖,测量各相关解剖结构到横窦下缘的距离。结果实验所收集的数据表明在定位横窦下缘和窦汇时,上项线及枕外隆凸尖不是一个可靠的浅表解剖学标志。而以半脊肌附着点作为浅表解剖学标志比以上项线作为浅表解剖标志更准确、可靠。本组10例标本中,无1例的半脊肌附着点超过横窦下部边界3mm,并且它可以作为一个可靠的浅表解剖学标志用以定位接近中线部分的横窦。结论这些发现可以帮助神经外科医生在治疗后颅窝和颅颈接合处病变而进行的各种中线入路手术时更准确的定位横窦。