药品包装材料对液体药剂质量的影响

目的:探讨药品包装材料与用药安全的关系。方法:参考有关文献并结合我国实际情况进行分析。结果:药品包装材料质量对人体能产生一定的危害,对药品质量也产生一定的影响。结论:应按药品的理化性质选用合适的药品包装材料,药品包装材料生产企业也要按专业标准化生产药品包装材料,以适应各种药品包装的需求。     

水发产品卫生学调查

[目的]了解水发产品的生产模式、加工工艺、水发剂使用的情况以及本市市售水发产品中甲醛与过氧化氢残留情况。[方法]用现场调查与抽样检验结合的方法,对市售水产品进行检测,对水发产品的生产模式、加工工艺、水发剂使用等情况进行调查。[结果]水发产品生产整体尚处于较低水平,调查中未发现有在水发过程中使用甲醛的情况,而使用过氧化氢的情况较为普遍,抽检的212件水发产品甲醛不合格率为9.0%,抽检的97件水发样品过氧化氢检出率为2.1%。[结论]产品中检出甲醛不合格率较高、过氧化氢检出率低;水发产品的原料中可能有甲醛本底的存在,而过氧化氢在产品中的残留相当不稳定。     

新型液相色谱-串联质谱法快速测定水产品中孔雀石绿及其隐色代谢物残留

目的采用液相色谱-串联质谱（LCMS／MS）建立水产品中孔雀石绿及其隐色代谢物残留的检测方法。方法均质后的水产品样品，用乙腈和乙酸铵缓冲液提取，合并提取液，用二氯甲烷反提取，经中性氧化铝柱和丙磺酸（PRS）柱固相萃取净化，没有使用氧化铅柱在线氧化，色谱分离后直接进入串联质谱检测器。采用电喷雾（ESI）正离子扫描模式，多反应监控（MRM）方式检测，外标法定量。结果孔雀石绿和隐色孔雀石绿的检出限为0．5ng／g，线性方程的相关系数r〉0．99，在添加浓度0．5～2．0ng／g范围内，孔雀石绿回收率为78．4％～92．1％。隐色孔雀石绿回收率为82．4％～98．1％，相对标准偏差（RSD）均小于5．7％。结论此方法高效快速，结果准确，重现性好，适合于对水产品中孔雀石绿和隐色孔雀石绿的残留检测。     

莱菔子不同炮制品对消化系统作用及其急性毒性研究

目的:研究莱菔子不同炮制品对消化系统药理作用及急性毒性。方法:分别采用小鼠胃排空和小肠推进及兔在体肠蠕动实验,研究莱菔子不同炮制品对消化系统的药理作用。结果:莱菔子不同炮制品对消化系统呈现不同药理作用。结论:莱菔子炮制得当,可更好地发挥药理作用。     

现代化是中药与植物药发展的必由之路

本文就我国中药与植物药如何逐步实现现代化提出一些管见,如什么是中药现代化的过程,中药现代化要从国内、国外两个层面着手,不同地区和不同厂家要扬长避短,选择优势项目等方面进行了论述,以供讨论。     

高速逆流色谱在天然产物分离中的应用

综述了高速逆流色谱在天然产物有效成分分离中的新应用.     

补脾肾活血法防治糖尿病肾病机理探讨

目的观察补脾肾活血方对糖尿病大鼠肾脏病变的影响,以探讨其防治糖尿病肾病的机制.方法用链脲佐菌素(STZ)制备糖尿病大鼠模型,12周后,测定肾功能指标、糖化血红蛋白(GHb)、血清和肾皮质糖基化终产物(AGEs)水平,并对大鼠肾脏进行光镜和电镜观察.结果补脾肾活血中药治疗后肾功能指标、血糖、糖化血红蛋白、血清和肾皮质AGEs都显著低于糖尿病组(P＜0.01 或 P＜0.05),且肾脏病理改变得以改善.结论补脾肾活血中药能够通过降低血糖、减少尿蛋白和抑制蛋白非酶糖化防治糖尿病肾病的发生.     

High mobility group box 1 (HMGB1) is upregulated by the Epstein-Barr virus infection and promotes the proliferation of human nasopharyngeal carcinoma cells

Conclusion: The current study confirmed the significant high mobility group box 1 (HMGB1) was promoted in human nasopharyngeal carcinoma (NPC) tissues by Epstein-Barr virus (EBV) infection, in association with the malignant status of NPC, and promoted the proliferation NPC cells RAGE-dependently. Objectives: The present study was to examine the association of HMGB1 over-expression in human NPC with the EBV-positivity and to determine the regulatory role of HMGB1 on the proliferation of NPC cells in vitro. Methods: Real-time PCR and Western blotting were utilized to examine the HMGB1 expression. EBV infection in CNE-2 cells was performed to investigate the HMGB1 promotion by EBV infection. RNA interference technology was utilized for the RAGE knockout. Results: It was demonstrated that HMGB1 was significantly higher in both mRNA and protein levels in the EBV-positive NPC tissues, in marked association with the malignant status of NPC, and with the LMP1 DNA level in EBV-positive NPC samples. In addition, the MTT assay, growth curve, and the colony forming assay confirmed the promotion by HMGB1 to the proliferation of CNE-2 cells, depending on RAGE.     

Correlation between the median particle size of chewed frankfurter sausage and almonds during masticatory performance test

The correlation between chewing and gastric function is best reflected when the same food type is used during both tests. We proposed frankfurter sausage as test food for masticatory performance as it can also be used in gastric emptying test. The suitability of frankfurter sausage to determine masticatory performance, however, has never been examined. To examine the correlations between the median particle size of frankfurter sausage and almonds (as standard test food) after different numbers of chewing cycles. Twenty‐seven subjects performed masticatory performance tests by chewing 2 types of test foods, that is, a piece of almond or 5‐g frankfurter sausage cubes placed in a sealed latex bag, for 5 and 15 chewing cycles. For each individual, right and left sides were tested separately. Chewed samples obtained from both sides were pooled. Median particle sizes were determined using a multiple sieving method. Spearman’s rank correlation was used to examine any correlation between median particle sizes of the 2 test foods after 5 and 15 cycles. Median particle sizes after 5 and 15 cycles were 2.04 ± 0.87 and 0.95 ± 0.58 mm for almonds and 4.16 ± 0.19 and 3.73 ± 0.25 mm for frankfurter sausage, respectively. Significant correlations were observed between the median particle size of chewed frankfurter sausage after 15 cycles and that of chewed almonds after 5 and 15 cycles (r = .76, P < .01 and r = .52, P = .01, respectively). Frankfurter sausage chewed for 15 cycles may be suitable for the determination of masticatory performance in conjunction with gastric emptying test.