The effect of hyperthermia on hepatic and tumor lysosome stability in rats with morris 7777 hepatomas

The stability of lysosomal membranes was examined during systemic hyperthermia (SH) in Morris 7777 hepatoma-burdened Buffalo rats, since previous studies have shown inhibition of growth patterns of this tumor with SH treatments. Cathepsin D and acid phosphatase were assayed from both ruptured and intact lysosomes from hepatoma and normal hepatocytes. No significant differences were found between results obtained from control animals and those with SH. Cathepsin D hepatoma lysosomes were found to be less stable than those of either normal or tumor-burdened rat hepatocytes. We conclude that lysosomes are relatively stable organelles that do not contribute to the inhibition of growth patterns seen with hyperthermia.     

Impression technique for complete maxillary immediate denture.

A technique for making complete maxillary immediate denture impressions has been described. With this procedure impressions of the edentulous area and remaining dentition can be made in sections and they can be removed from the mouth joined together.     

Erythrocyte sodium-potassium-stimulated adenosine triphosphatase activity is not related to obesity

Altered erythrocyte sodium potassium (Na,K)-stimulated adenosine triphosphatase (ATPase) activity has been cited as having pathophysiologic significance in morbidly obese man. Previous studies have failed to consider obese patients after weight loss and, therefore, did not clarify the role of ATPase deficiency as a cause or effect of the obese state. To define more completely the possible alteration of cellular thermogenesis in obesity, a study was made of three groups of people: (1) normal weight controls; (2) morbidly obese; and (3) formerly morbidly obese patients who had lost over 100 pounds after gastric bypass surgery. Erythrocyte ATPase activity was determined by use of an assay that coupled ATPase activity with NADH oxidation in the presence of excess pyruvate kinase, lactic dehydrogenase, and phosphoenolpyruvate. This coupled assay produced a continuous slope so that activity could be calculated from the initial, maximal, linear portion of the decay trace. Results did not demonstrate any statistically significant differences in Na,K-ATPase activity between groups by analysis of variance. A nonsignificant correlation of 0.086 was seen between obesity index and Na,K-ATPase activity. It is concluded that (1) erythrocyte Na,K-ATPase activity is similar in both normal and obese individuals, (2) erythrocyte Na,K-ATPase does not change with weight loss, and (3) therefore, disordered erythrocyte thermogenesis does not have a role in the development or maintenance of obesity.