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991.
未研究发现,经热休克处理的大鼠注油酸后,肺组织损伤明显减轻,Western印迹分析显示热休克使大鼠肺组织中HSP70含量明显增多;Northern印迹分析证实此乃由于HSP70mRNA转录增加所致。说明热休克处理使肺组织HSP70基因表达增强;提示热休克反应对大鼠油酸肺的保护作用与肺组织细胞中HSP70基因表达增强及与HSP70增多有关。  相似文献   
992.
Non-invasive ambulatory recordings of blood pressure and heart rate were performed using a Spacelabs device during day and night periods in patients with Parkinson's disease with (n = 19) or without orthostatic hypotension (n = 19). In patients with orthostatic hypotension, the average systolic and diastolic blood pressure during the night (137 ± 5/80 ± 3 mmHg) was higher (p < 0.05) than during the day period (121 ± 3/76 ± 2 mmHg). In patients without orthostatic hypotension, a decrease in blood pressure was recorded during the nocturnal period. In patients with orthostatic hypotension, the blood pressure variability was higher (p < 0.05) during the day (systolic: 14.6 ± 1.3%; diastolic: 16.5 ± 1.0%) than during the night (systolic: 9.1 ± 0.8%; diastolic: 10.8 ± 1.1%). The blood pressure load (percentage of values above 140/90 mmHg) during the night was significantly higher than during the day for both systolic (41.2 ± 8.1 vs. 19.6 ± 4.7%) and diastolic blood pressure (24.9 ± 6.9 vs. 16.3 ± 4.9%). There was a decrease in heart rate in both groups during the night. A fall of 25 mmHg or more in systolic blood pressure after meals occurred in ten patients with orthostatic hypotension and in one patient without orthostatic hypotension. These results indicate that orthostatic hypotension in Parkinson's disease is associated with specific modifications of ambulatory blood pressure including loss of circadian rhythm of blood pressure, increased diurnal blood pressure variability and post-prandial hypotension.  相似文献   
993.
心脏病患者血清七种元素含量与血压及生化指标的关系   总被引:31,自引:0,他引:31  
我们检测了心脏病患者血清微量元素Zn、Cu、Fe和常量元素K、Na、Ca、Mg的含量,并与血压及生化指标作相关分析。结果发现心脏病患者K和Ca/Mg显著低于对照组,而Na、Mg、Zn、Fe和Na/K、Zn/Cu则显著高于对照组。K、Ca/Mg与血压呈负相关,而Na、Zn、Fe、Zn/Cu和Na/K与血压呈正相关;Fe与红细胞压积(Hct)呈正相关;Zn/Cu与Hct、甘油三酯、极低密度脂蛋白均呈正相关,而与高密度脂蛋白呈负相关。  相似文献   
994.
本文对300例肛肠疾病采用CO_2激光治疗,治愈率为94.33%,一次切割完成,不需止血缝合,一般7-15天痊愈,疗效明显优于其它治疗方法。  相似文献   
995.
Summary A new double-staining procedure, in which the techniques of immunocytochemistry of glial fibrillary acidic protein (GFAP) and quantitative microdensitometry of azure B-RNA were combined, was used to study nucleic acid alterations in fibrous astrocytes in Alzheimer's disease (AD). RNA contents of GFAP-positive cells of the hippocampal endplate (Rose's H3-H5 fields) and the dentate gyrus molecular layer were determined in ten autopsy-proven AD patients (ages 51–88) and ten age-matched, non-demented control. In addition, RNA contents of pyramidal neurons of the endplate were examined. While there were no differences in RNA contents of astrocytes of either region between AD patients and controls, neuronal RNA was markedly depleted. These data suggest that astrocytes maintain protein synthetic capabilities in AD and that RNA loss is limited to the neuronal compartment.Supported by Grants 1P01-AG05119 and 1P50-AG05144 from the National Institutes of Health and by a Small Research Project Award from the University of Kentucky Medical Center  相似文献   
996.
The pathogenesis of myasthenia gravis (MG) involves a T cell-dependent antibody-mediated autoimmune response directed against acetylcholine receptors (AChR). Inactivation of AChR-specific T cells should interrupt the immune response, resulting in therapeutic benefit. Since each individual's repertoire of T cells responds to a heterogeneous and unique spectrum of AChR epitopes presented in association with self-major histocompatibility complex (MHC) class II, an individualized approach is required to target all relevant AChR-specific T cells. The individual's own antigen-presenting cells (APC) can be used for this purpose, since they process and present the antigen appropriately, and express the correct MHC class II. A novel method of binding AChR to surface immunoglobulin with a heterobifunctional antibody conjugate allows us to use all B cells as APC. Conjugate-plus-AChR-treated B cells (AChR-APC) effectively targeted AChR-specific T cells, stimulating vigorous proliferative responses in a rat cell culture system. If APCs are 'fixed' with cross-linking reagents, they induce long-lasting or permanent 'anergy' of the specific T cells. We prepared AChR-APC, allowed them to process AChR in vitro, and fixed them with paraformaldehyde. Pre-culture of these fixed AChR-APC with AChR-specific T cells induced anergy: when restimulated with fresh AChR-APC, the T cells exhibited markedly reduced proliferative responses and IL-2 production, compared with responses of T cells pre-cultured with control fixed B cells. Implications for the design of antigen-specific therapeutic strategies for MG and other immune disorders will be discussed.  相似文献   
997.
载脂蛋白B信号肽序列多态性及其与冠心病的关联   总被引:4,自引:0,他引:4  
应用聚合链反应(PCR)对103例冠心病人及100名正常人载脂蛋白B(apoB)基因5'端信号肽序列的多态性研究结果表明:(1)中国人信号肽序列插入/缺失(Ins/Del)多态性频率分布与国外不同种族间有差异。少见Del等位基因相对频率为0.259,高于南亚人,但低于法国高加索人。(2)冠心病组与正常对照组间等位基因相对组间等位基因相对频率分布差异无显著意义。(3)冠心病组内具Del等位基因者血浆  相似文献   
998.
郑景浩  苏肇伉 《上海医学》1995,18(3):128-130
小儿先心病术后左房夺和肺动脉压力的监测在ICU中十分重要。心电图是论断和评价心脏疾患的常用方法。本文分析了32例小儿先心病手术前后(伴肺动脉高压16例)6个肢体导联心电图中P波和QRS波时限总和及振幅总和与左房、肺动脉压力的关系。结果表明两者均有良好的相关性(r=0.85 ̄0.88,r=0m74 ̄0.80,P〈0.01),该法简单、方便,可广泛开展,尤其在术后未放置PA和LA管或在IOU中已拨除L  相似文献   
999.
Acute renal failure and acute heart failure are rare in Kawasaki disease. We experienced two patients with Kawasaki disease who presented acute renal failure and acute heart failure. These two patients gave us an important insight into the understanding of water balance and fluid therapy in Kawasaki disease. One patient showed acute prerenal failure due to fluid exudation from the intravascular to the extravascular space, and subsequent acute heart failure. The other patient showed acute heart failure caused by fluid infusion for the treatment of dehydration. It is suggested that acute renal failure could be caused by a fluid shift from the intravascular to the extravascular space in Kawasaki disease. It is also demonstrated that the reserve of cardiac function could be decreased in patients with Kawasaki disease due to myocarditis even with normal echocardiography and chest X-rays.  相似文献   
1000.
The monoclonal antibody Alz-50 is directed against Alzheimer's disease-related modified tau proteins and reveals cytoskeletal changes, i.e. neurofibrillary tangles and dystrophic neurites. The present study shows that, in the hypothalamus of non-demented control subjects, this same antibody gives a distinctive staining pattern of a subpopulation of somatostatin neurons and beaded fibers. Furthermore, Alz-50 occasionally recognizes somatostatin-containing cell bodies and dystrophic neurite-like fibers in the (neuritic) senile plaques of AD patients. These observations have direct consequences for the interpretation of Alz-50 staining in diagnostic usage and for the assessment of Alzheimer's disease-like changes induced by β-amyloid in experimental animal brains. On dot spotting, Alz-50 was found to bind to a number of fragments from the somatostatin precursor, of which somatostatin15–28 stained best. Preadsorption of Alz-50 by somatostatin15–28, as well as other specificity tests, failed, however, to provide any clue to the nature of the unknown compound(s) stained in the control hypothalamus.  相似文献   
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