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161.
目的 对比应用逆行交锁髓内钉(GSH)和动力髁螺钉(DCS)内固定治疗股骨远端骨折的效果.方法 采用回顾性研究方法对我院自2000年8月~2004年10月治疗的54例股骨远端骨折病例进行对照研究,按A0分类:A1型17例,A2型14例,A3型11例,B1型2例,C1型6例,C2型3例,C3型1例.其中,DCS内固定30例,GSH固定24例.分组统计手术时间,出血量,骨折愈合时间,并发症,膝关节功能.结果 所有病例经5月~2年随访,平均9个月;骨折愈合时间为4.6月(4~9月).DCS固定组平均手术时间1.5小时(1~2.5小时).出血量105 ml(50~200 ml),骨折愈合时间平均4.7月(4~8月),并发症发生5例(发生率16.7%),根据Kolmert膝关节功能评分,优20例,良6例,可4例,优良率为86.6%.GSH固定组手术时间1.8小时(1.5~3.5小时).术中出血量平均120 ml(100~250 ml),平均骨折愈合时间5.1个月(4.5~9个月),并发症发生5例(发生率28%),根据Kolmert膝关节功能评分标准,优15例,良7例,可2例,优良率为91.7%.两组比较,结果无显著差异(P>0.05) 结论 逆行带锁髓内钉(GSH)及动力髁螺钉(DCS)均是治疗股骨远端骨折的较好方法,但各有其最佳适应症.选择好适应症以及术者技术熟练有利于骨折愈合及关节功能恢复. 相似文献
162.
163.
RNA was extracted from tissues processed by a new fixation and paraffin-embedding method (the AMeX method) and examined by Northern blot analysis and slot-blot analysis. The RNA extraction method for AMeX-processed tissue sections after the deparaffinization step was the same as that for fresh materials. The total amount of cellular RNA extracted from AMeX-processed mouse liver tissue was slightly less than that extracted from fresh tissue. In tissues of malignant lymphoma, the total amount of cellular RNA extracted from 25 sections each 20 microns thick was about 1.6-1.8 micrograms/mm2, regardless of the histological subtype and period of storage. The extracted RNA was moderately degraded, and usually could not be used for Northern blot hybridization analysis. The intensity of ethidium bromide staining and the hybridization signals of RNA extracted from AMeX-processed tissues were usually reduced in comparison with RNA from fresh material, but specific signals could be detected by slot-blot hybridization analysis. We have demonstrated previously that the AMeX method preserves high-molecular-weight DNA and various antigens. Since the present study showed that information on mRNA can be obtained from AMeX-processed tissue, the versatility and usefulness of this method were further proven. 相似文献
164.
C. K. Peck J. A. Baro 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1997,113(2):291-302
Neurons in the rostral superior colliculus (SC) of alert cats exhibit quasi-sustained discharge patterns related to the fixation
of visual targets. Because some SC neurons also respond to auditory stimuli, we investigated whether there is a population
of neurons in the rostral SC which is active in relation to fixation of both auditory and visual targets. We identified cells
which were active with visual fixation and which continued to discharge if the fixation stimulus was briefly extinguished.
The population of neurons exhibited similar discharge characteristics when the fixation stimulus was auditory. Few neurons
were significantly more active during fixation of visual targets than during fixation of auditory targets. Most fixation neurons
showed a diminished discharge rate during spontaneous (self-generated) saccadic eye movements away from a visual fixation
stimulus, regardless of the direction of the saccade. this diminished discharge rate (or pause) typically began, on average,
12.2 ms before saccade onset and the duration of the pause was Ionger than the duration of the saccade. These observations
are consistent with the hypothesis that increased discharge of these neurons is related to active fixation and that reductions
in their activity are important for the generation of saccades. However, the lack of a precise relationship between pause
duration and saccade duration implies that these neurons would be unlikely to project directly to the saccadic burst generator.
The mean interval from the beginning of the pauses of fixation neurons to be beginning of the saccades away from fixation
targets is also shorter than has been found in brainstem omnipause neurons. By analogy with the concept of a receptive field,
agaze position error field depicts the range of gaze position error for which a cell is active. Although fixation neurons appear to encode the magnitude
and direction of the error between visual targets and the visual axis, visual error fields at the end of fixating eye movements
were significantly larger than those at stimulus onset. For auditory stimuli, this difference was not significant. These observations
are compatible with a number of recent experiments indicating that neural signals of eye position are damped or delayed with
respect to current eye position. 相似文献
165.
股骨粗隆间骨折(intertrochanteric fracture of femur,IFF)占髋部骨折的近一半,多见于老年患者。IFF的治疗方式包括保守治疗和手术治疗。回顾髓内固定治疗股骨粗隆间骨折的相关文献并分析各种治疗方法的临床应用、优势、手术难度及并发症。总结髓内内固定治疗股骨粗隆间骨折进展。 相似文献
166.
逆行带锁髓内钉治疗股骨远端骨折的应用解剖 总被引:6,自引:0,他引:6
目的:为带锁髓内钉治疗股骨远端骨折提供形态学基础。方法:①选用30例成人干燥股骨测量股骨内外侧髁、内外上髁、髁间窝有关数据进行统计分析。②20侧下肢标本解剖观测膝降动脉及隐神经髌下支的分支、分支等及两者的关系。结果:髁间窝呈U型占35%,V型占65%,此窝最宽处横径:左侧(1.98±0.36)cm, 右侧(2.06±0.29) cm;内侧髁后关节面宽,左侧(2.21±0.23) cm,右侧(2.34±0.29) cm;内、外上髁的距离,左侧(7.30±0.60)cm,右侧(7.43±0.57)cm。膝降动脉髌下支和隐神经髌下支呈紧密的伴行关系。隐神经在缝匠肌中下1/3交界点穿出,有上、下两支分布到膝关节前外侧皮肤。结论:术中注意这些解剖位置特点,避免损伤神经和血管,减少手术出血,防止并发症。 相似文献
167.
The effect of a series of fixatives on the AgNOR technique 总被引:6,自引:0,他引:6
With increasing interest being shown in nucleolar organizer regions (NORs) in pathology, it was considered of great importance to evaluate the effect of some of the more commonly used and more specialized fixatives on the demonstration of these moieties. NORs can be demonstrated in paraffin sections by a silver technique (AgNOR method) which was developed from a method used by cytogeneticists for the demonstration of NORs in chromosome spreads. The degree of staining is dependent on the fixation regime employed and results may vary greatly from one fixative to another. The fixative schedules and post-treatments used in this study were based on standard sequences from the literature. We have shown that, in general, alcohol-based fixatives give optimal results, Carnoy's fluid being especially recommended. Mercurial and dichromate-containing fixatives were found to have highly detrimental effects on NOR staining. 'Routine' 10 per cent formol saline fixation gave adequate results whereas 10 per cent neutral buffered formalin gave optimal staining, similar to alcohol-based fixation. 相似文献
168.
Development of a competitive enzyme-linked immunosorbent assay for detecting cytomegalovirus antibody 总被引:4,自引:0,他引:4
A competitive enzyme-linked immunosorbent assay (ELISA) for detecting cytomegalovirus (CMV) antibody was developed. The competitive ELISA was five times more sensitive than the complement fixation test (CFT) and twice as sensitive as indirect ELISA. Testing of paired sera from cardiac transplant patients taken before and after transplantation showed good correlation between results of competitive and indirect ELISA and CFT. The competitive ELISA was more successful than CFT or indirect ELISA in detecting passively acquired antibody, but detection of CMV antibody by competitive ELISA immediately after primary CMV infection was unreliable, possibly because of the high affinity of the monoclonal antibody chosen for the horseradish peroxidase conjugate. However, competitive ELISA may well prove to be more suitable than indirect ELISA for detecting CMV antibody in blood donations. 相似文献
169.
椎弓根螺钉在胸腰椎骨折治疗中体会 总被引:3,自引:0,他引:3
目的探讨椎弓根螺钉(PS)在胸腰椎骨折治疗中的疗效。方法回顾性分析应用PS治疗胸腰椎骨折的68例病例资料。全部病例均采用分离显露损伤椎体及上下各一个椎体行后路减压,椎弓根螺钉复位内固定,小关节突及横突间和椎间植骨融合的治疗方法。结果随访2-52个月,68例骨折均愈合,58例椎体高度完全恢复,压缩性骨折中6例残留10%-30%的压缩未能恢复,65例植骨融合良好,无内固定松动现象。结论椎弓根是脊柱中最坚强的部位,椎弓根螺钉经椎弓根至椎体固定胸腰椎骨折操作简便、固定可靠、骨折复位和恢复胸腰椎正常弧度可以分步骤完成,重建的脊椎稳定性好,可早期下床活动,为目前胸腰椎骨折治疗中较好的方法之一。 相似文献
170.
Ectodermal dysplasias (EDs) are developmental disorders affecting tissues of ectodermal origin including hair, nails, teeth and sweat glands. Ectodermal dysplasia of hair, nails and teeth is a rare type of congenital disorder characterized by sparse and thin hair, dystrophic finger-and toenails and missing and abnormal teeth. In an effort to understand the molecular basis of this form of ED a family of Pakistani origin with an autosomal recessive pattern of inheritance was ascertained from a remote region in Pakistan. The clinical features of the affected individuals included thin and fine hair on the scalp, dystrophic and flat nails, absent or sparse eyebrows and eyelashes, missing and abnormal teeth, and thin body hair. A human genome scan carried out using microsatellite markers mapped the disease locus in this family to chromosome 18q22.1–18q22.3. A maximum two-point LOD score of 2.73 (θ= 0.0) was obtained at marker D18S541. Multipoint linkage analysis resulted in a maximum LOD score of 3.42 obtained with several markers, including D18S1125, ATA82B02, D18S848, D18S488, D18S1091, and D18S485, which supported the linkage. The linkage interval is flanked by markers D18S857 and D18S815, which corresponds to a region of 17.32 cM according to Rutgers combined linkage and physical map (build 36). This region covers 8.63 Mb according to the sequence-based physical map. Three candidate genes, CDH7, CDH19 and ZNF407 , from the linkage interval were sequenced and found to be negative for functional sequence variants. This study is the first step towards the identification of a gene involved in hair, nails and teeth type ED. 相似文献