Application of HILIC stationary phase to determination of dimethindene maleate in topical gel

A novel high performance liquid chromatography method for the determination of dimethindene maleate in pharmaceutical gel using hydrophilic interaction liquid chromatography (HILIC) with UV detection was developed and validated.     

Carrier analysis of a moderately affected haemophilia B family.

Here we report the successful genetic diagnosis of a pregnant caucasian female patient whose family has a history of moderate haemophilia B. While restriction fragment length polymorphism (RFLP) analysis was not informative, nucleotide sequencing of the factor IX genes of the patient's family members determined that her mother and one of her two sisters were carriers of the mutation C31008T, which causes a Thr296Met transition. In contrast, the pregnant female herself and her other sister were found to carry only normal alleles. Plasma factor IX activity and antigen levels supported these findings.     

E-选择素、CEA联合检测对良恶性胸腔积液的鉴别

目的　探讨血清和胸腔积液中E -选择素 (E -selectin或E -SLT)、癌胚抗原 (CEA)及胸腔积液细胞学多项指标联合检测对结核性和恶性胸腔积液的诊断价值。方法　对 35例结核性胸腔积液、35例恶性胸腔积液及 2 0例健康人的血清和胸腔积液进行检测。结果　恶性胸腔积液组血清和胸液中E -SLT低于结核性胸腔积液组 ,而CEA则前者高于后者 ,差异均有高度显著性。以胸液E -SLT >2 3ng/ml为结核组的界值 ,单项指标诊断结核性胸腔积液的敏感性为 91.4 % ,特异性 71.4 % ,准确性 81.4 %。以血清CEA 5 μg/ml为恶性胸腔积液界值 ,敏感性为 6 8.6 % ,特异性 91.4 % ,准确性 80 %。联合上两项指标加胸腔积液细胞学检查 ,敏感性 97.1% ,特异性 10 0 % ,准确性 98.6 %。结论　E -SLT及CEA联合检测对良恶性胸腔积液的鉴别有重要意义。     

Effects of background suppression of gamma counts on signal estimation

Gamma detectors at border crossings are intended to detect illicit nuclear material. One of their performance challenges is the fact that vehicles suppress the natural background and, thus, potentially reduce probability of detection of threat items. Here we test several methods to adjust the detection to background suppression in the context of signal estimation.We show that, for the small-to-moderate suppression magnitudes, suppression adjustment leads to higher detection probability. However, for signals triggering alarm without an adjustment, adjustment does not improve estimation of the signal location, only moderately improves estimation of the signal magnitude, and does not improve estimation of the signal width.     

用la和OKT单克隆抗体研究胃癌和食管癌患者的免疫功能

用Ia和OKT单克隆抗体对经纤维胃镜确诊的食管癌17例、胃癌20例及正常人17名的外周血淋巴细胞进行分类。空腹抽血5ml,提取淋巴细胞,用Ia、OKT_3、OKT_4、OKT_8抗体和兔抗鼠IgG荧光抗体作免疫荧光染色。计数100个淋巴细胞中阳性细胞的百分比。结果:正常人T淋巴细胞(OKT_3阳性)为74.03±2.19,辅助性T淋巴细胞(OKT_4阳性)为49.02±3.88,抑制性T淋巴细胞(OKT_8阳性)为28.98±3.89,B淋巴细胞(Ia阳性)为20.86±2.98,OKT_4/OKT_8比值为1.72。食管癌患者与胃癌患者的OKT_4为41.27±5.98与39.33±7.29,明显低于正常人组;OKT_8为40.09±6.11与36.26±7.21,较正常人组显著增加;OKT_4/OKT_8比值为1.10与1.15,显著小于正常人组。说明进展期肿瘤患者的免疫功能均处于失调状态。     

通用复合数据巡检网络的构建

本文提出了一种复合数据采集网络的构建方法,采用视频图像识别技术和无线串行通讯技术对大量实验设备输出结果进行数据采集。系统通过对视频图像的分割和插值放大处理后得到字模图像,进而与字库图像比对后得到图像中的数符串。同时系统采用仿真串口实现对具体设备数据的读取,并通过构建的无线网络进行串行数据采集。本系统适用于对大量实验数据的自动检测与汇总。     

高效液相色谱法测定化妆品中三氯生

采用高效液相色谱（ＨＰＬＣ，带有二级管阵列检测器）测定化妆品的杀菌剂三氯生。用Ｃ１８柱，以乙腈－水（６０：４０），用冰醋酸调节ｐＨ值３．０为流动相，检测波长２８０ｎｍ，以保留时间及紫外光谱图作定性，以峰面积或峰高作定量．该方法准确度和精密度较高，各类化妆品加标回收率范围为９１．９％～９８．８％，相对标准偏差范围为０．７２％～２．８％。     

FDG双探头符合成像与X线断层图像同机融合在胸腹部肿瘤病变诊断中的价值

目的 :初步评价FDG双探头符合成像与X线断层图像同机融合在胸腹部肿瘤病变诊断中的价值。材料和方法 :回顾性分析 2 2例疑为胸腹部肿瘤或肿瘤术后复发而行18F -FDG双探头符合成像与X线断层联合扫描同机图像融合患者的资料。结果 :14例肿瘤或肿瘤术后复发的患者FDG显像 ,在 34个病灶中共检出 33个病灶 ( 97.1% )。融合影像可更加直观清晰地显示占位性病变或肿大淋巴结的代谢活性。其中 4例 ( 4 /14 )患者位于邻近膈肌上下及胸椎体、前列腺的 6个病灶 ( 6 /34)必须借助融合图像才能精确定位。结论 :FDG双探头符合成像与X线断层图像同机融合可明显提高符合显像定位诊断的准确性。