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871.
872.
作者利用其采用PCR技术从恙虫病立克次体基因组中扩增出Sta58主要抗原基因的部分片段,约1kb,克隆入质粒pSP72的BamHI、SmaI位点,建重组质粒pSPRK9,又将pSPRK9中的AccI小片段约500bp亚克隆至pSP72构建重组质粒pSPRK7。以上无性繁殖系的建可为我国开展恙虫病立克次体核酸杂交研究提供特异核酸探针。 相似文献
873.
胃肠安对人胃癌细胞SGC-7901原位移植瘤细胞增殖和凋亡的影响 总被引:1,自引:0,他引:1
背景:以健脾类中药为基础的复方胃肠安可抑制人胃癌细胞SGC-7901裸小鼠皮下移植瘤细胞的增殖,并诱导细胞凋亡,提高进展期胃癌的3年生存率。目的:通过观察胃肠安对人胃癌细胞裸小鼠原位移植瘤生长和凋亡的影响,探讨其抑制肿瘤的作用机制。方法:以高效液相色谱(HPLC)法监测复方胃肠安制剂的稳定性;采用胃小囊法,予45只雄性BALB/c-nu/nu裸小鼠接种人胃癌细胞SGC-7901移植瘤模型,造模第28天,选出扪及胃部肿块直径为3mm左右的裸小鼠共43只,随机分为胃肠安组(14只)、5-氟尿嘧啶(5-FU)组(14只)和对照组(15只);采用免疫组化SP法检测增殖细胞核抗原(PCNA)的表达;采用缺口末端标记(TUNEL)法检测细胞凋亡指数。结果:胃肠安组原位瘤抑制率为40.8%,肝转移率(7.7%)和总转移率(30.8%)显著低于对照组(50.0%和71.4%)(P<0.05);胃肠安组原位移植瘤PCNA阳性率和总阳性率(51.0%±11.4%和60.4%±13.3%)显著低于对照组(61.6%±11.6%和74.4%±7.9%)(P=0.0287和P=0.0029);胃肠安组细胞凋亡指数(17.3%±8.9%)显著高于对照组(7.8%±3.0%)(P=0.0010)。结论:复方胃肠安可抑制人胃癌细胞裸小鼠原位移植瘤的生长和转移,并可抑制人胃癌细胞原位移植瘤的细胞增殖和诱导凋亡。 相似文献
874.
Immunoglobulin profiles in a murine intermediate host model of resistance for Echinococcus granulosus infection 总被引:3,自引:0,他引:3
Zhang W You H Li J Zhang Z Turson G Aili H Wang J McManus DP 《Parasite immunology》2003,25(3):161-168
We have shown previously that primary infection of Chinese Kunming (CKM) mice with Echinococcus granulosus oncospheres is protective against subsequent challenge. Nine groups of mice were infected with the oncospheres of E. granulosus by different routes (intraperitoneal, subcutaneous or intravenous injection). After infection, serum was collected after different periods of time and serum antibodies were tested by ELISA against oncospheral proteins and hydatid cyst fluid antigens. The results indicated that CKM mice produced low levels of antibodies before a secondary challenge infection given 3 weeks later by a different route. Most mice did not evoke significant antibody responses against oncospheral antigens until 5 weeks after infection. The level of IgG, especially IgG1 against oncospheral antigens increased from week 4 post-infection (p.i.), to a maximum at week 9 p.i. In addition, antibodies against hydatid cyst fluid antigens increased at the same time as the recognition of oncospheral antigens. Immunoblots using hydatid cyst fluid showed that the first antigen that was recognized - an 8-kDa protein, possibly the smallest subunit of Antigen B - appeared 5-6 weeks p.i. and reactivity to this molecule was intensive at week 9 p.i. The results suggest that protection against secondary infection was not principally antibody-mediated during the initial phases of infection, when cellular immune responses may play a pivotal role in the protective mechanism. 相似文献
875.
876.
Summary Rabbits were first immunized and later challenged with the same antigen (bovine serum albumin, or ferritin) by injection into the tibialis anterior tendon. Inflammatory changes of the tenosynovium and epitenon included infiltration by neutrophils (early) and mononuclear cells (later) over a 6-week course of tenosynovitis. A pattern of antigen entrapment in the tendon together with immunoglobulin was shown by use of radiolabelled antigen and immunochemical staining. Half-life of antigen in the tissues averaged 5 days over the 6-week period. Changes in the epitenon included cellular necrosis, appearance of phagocytic cells, and disruption of the collagen matrix. Tissues of control animals (challenged without prior immunization) showed minimal changes and significantly less retention of antigen (P>0.005). The model is relevant to the mechanism of tendon damage associated with antigen-driven chronic inflammation, as may be the case in rheumatoid arthritis. 相似文献
877.
结核分支杆菌Ag85 B分泌蛋白基因疫苗的构建和免疫原性的研究 总被引:17,自引:1,他引:17
目的:构建编码结核分支杆菌(MTB)Ag85B分泌蛋白的重组真核表达质粒,并研究其免疫原性。方法:采用聚合酶链反应(PCR)方法从结构分支杆菌H37Ra基因组DNA中扩增出Ag85B分泌蛋白基因,用HindⅢ和EcoRⅠ消化后,与同样酶消化的pcDNA3连接,转化大肠杆菌JM109,阳性克隆用酶切鉴定;重组表达质粒肌注免疫小鼠4周后,分别用dot blotting和ELISA方法检测抗体的产生和滴度,结果:酶切监定重组表达质粒pTB30s构建成功,dot blotting检测免疫小鼠血清抗Ag85B特异性抗体阳性,ELISA检测抗体几何平均滴度为1:120。结论:应进一步研究pTB30s刺激机体的细胞免疫应答,以用于结核病(TB)的防治研究。 相似文献
878.
目的探讨特发性扩张型心肌病(idiopathic dilated cardiomyopathy,IDC)易感的分子机制和确立一种人类主要组织相容性复合体(HLA—A*)基因的检测方法。方法采用聚合酶链式反应和顺序特异性引物(PCR—SSP)基因分析方法,对31例特发性扩张型心肌病患者及29例无血缘关系的健康人的HIA—A*各等位基因及亚基因进行检测分析,并将该方法与其他检测HIA等位基因的方法进行对比。结果HIA—A* 03基因与IDC呈正相关(RR=4.697,P〈0.05),其他HIA—A*各等位基因未见异常。结论HIA—A*03基因可能是北方汉族人IDC的致病易感基因之一。采用的方法(PCR—SSP)具有快速、简便、敏感、准确和可靠等优点,值得推广。 相似文献
879.
Julio Núñez Pau Llàcer Eduardo Núñez Silvia Ventura Clara Bonanad Vicent Bodí Gema Miñana Enrique Santas Beatriz Mascarell Gregg C. Fonarow Francisco J. Chorro Juan Sanchis 《International journal of cardiology》2014
Background
The use of loop diuretics in acute heart failure (AHF) is largely empirical and has been associated with renal function impairment by reducing renal perfusion but also renal improvement by decreasing renal venous congestion. Antigen carbohydrate 125 (CA125) has emerged as a proxy for fluid overload. We sought to evaluate whether the early changes in creatinine (ΔCr) induced by intravenous furosemide doses (ivFD) differ among clinical groups defined by overload status (CA125) and creatinine on admission (Cr).Methods and results
We included 526 consecutive patients admitted for AHF. All patients received intravenous furosemide for the first 48 hours. CA125 and Cr were dichotomized at 35 U/ml and 1.4 mg/dl, respectively, and grouped as follows: C1 [Cr < 1.4, CA125 ≤ 35 (n = 151)]; C2 [Cr < 1.4, CA125 > 35 (n = 241)]; C3 [Cr ≥ 1.4, CA125 ≤ 35 (n = 45)]; and C4 [Cr ≥ 1.4, CA125 > 35 (n = 89)]. Clinicians in charge of the management of patients were blind to CA125 values. ΔCr was estimated as the absolute difference in Cr between admission and 48–72 hours. Multivariable linear regression analysis was used for modeling purposes. The adjusted analysis showed a differential effect of ivFD on ΔCr. Per increase in 20 mg/day of ivFD, the mean ΔCr was 0.010 mg/dl (p = 0.464) in C1, 0.002 mg/dl (p = 0.831) in C2, 0.045 mg/dl (p = 0.032) in C3, and − 0.045 mg/dl (p < 0.001) in C4 (omnibus p < 0.001). A similar pattern of response was observed in a validation cohort.Conclusions
In patients with AHF, the magnitude and direction of ΔCr attributable to ivFD were differentially associated with values of CA125 and Cr on admission. 相似文献880.
目的对MPT63、Ag85A、RV2031c和融合蛋白CFP10-ESAT6四种结核分枝杆菌抗原进行诊断效能评价,优选出最佳的抗原组合,分析其应用价值。方法基于酶联免疫吸附试验,分别用MPT63、Ag85A、RV2031c和融合蛋白CFP10-ESAT6四种重组结核抗原,对199份血清(含94份结核病患者血清和105份健康者血清)进行检测。结合受试者工作特征曲线法计算不同抗原的最佳临界值(cut-off value)并以此确立各抗原组合的灵敏度、特异度、阳性预测值、阴性预测值和诊断效率,对它们的诊断效能和应用价值进行综合评价。结果 11种组合中Rv2031c与CFP10-ESAT6的组合诊断效能最好,灵敏度为63.8%,特异度为82.9%,阳性预测值为76.9%,阴性预测值为71.9%,诊断效率为73.9%。其余组合的灵敏度的范围30.9%~66%,特异度范围76.2%~90.5%。结论 Rv2031c和CFP10-ESAT6组合后的诊断效能最佳,有一定的临床应用价值。 相似文献