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161.
金标免疫层析法测定前列腺抗原方法的应用 总被引:3,自引:0,他引:3
目的:采用前列腺抗原(PSA)单抗标记胶体金的方法。测定PSA抗原。方法:采用柠檬酸三钠还原法制备胶体金、金标免疫层析方法(GSLIA)测定。结果:将金标方法与ELISA方法进行比较灵敏度、特异性、其两者无显著性差异。金标方法可在室温放置1年以上。结论:金标免疫层析法测定前列腺抗原,是一种快速、简便、灵敏的筛选前列腺癌患者的诊断方法。 相似文献
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164.
《Journal of labelled compounds & radiopharmaceuticals》2005,48(2):85-100
Asymmetric synthesis of AMPA potentiator LY450108‐[14C] containing 14C‐label attached to the chiral center of the molecule, was accomplished based on Evans' chiral oxazolidinone auxiliary method. Diastereoselective methylation of p‐nitrophenylacetic acid derivative was used as a key step. The auxiliary was reductively removed, and the resulting primary alcohol was converted into the corresponding amine. Its sulfonylation, reduction of the aromatic nitro group, and acylation with 3,5‐difluorobenzoyl chloride led to the final product. The synthesis of tritiated LY450108 is also detailed. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献
165.
Yuk Fai To Raymond Wai‐Yin Sun Yongxiong Chen Vera Sau‐Fong Chan Wing‐Yiu Yu Paul Kwong‐Hang Tam Chi‐Ming Che Chen‐Lung Steve Lin 《International journal of cancer. Journal international du cancer》2009,124(8):1971-1979
Nasopharyngeal carcinoma (NPC) is a common neoplasm in Southeastern Asia, and cisplatin‐containing regimens for combinational chemotherapy are widely used for treating locally recurrent or metastatic diseases. However, resistance to cisplatin is not infrequently seen and its associated side effects may be life‐threatening. In this report, another metallo‐pharmaceutical agent gold(III) porphyrin complex [Au(TPP)]Cl was investigated in comparison to cisplatin for its in vitro and in vivo anticancer effects. Through induction of the intrinsic apoptosis pathway, [Au(TPP)]Cl exhibited 100‐fold higher potency than cisplatin in killing NPC cells, including cisplatin‐sensitive and cisplatin‐resistant variants, and also an variant harboring the Epstein‐Barr virus. In addition, a safety concentration window was demonstrated, allowing [Au(TPP)]Cl to kill tumors with minimal cytotoxicity to noncancerous cells. More importantly, weekly intraperitoneal injection of 3 mg/kg [Au(TPP)]Cl was more effective than the same dose of cisplatin in inducing tumor apoptosis in vivo and remarkably inhibited tumor growth in animals without any noticeable side effect. [Au(TPP)]Cl therefore is a promising chemotherapeutic agent that deserves further development as a novel drug for the treatment of advanced NPC, in particular, for cases with cisplatin‐resistance. © 2008 Wiley‐Liss, Inc. 相似文献
166.
Peripheral facial paralysis is often accompanied by incomplete closure of the lid. In the past, tarsorrhaphy has been the
primary method for achieving improved eyelid closure, but it has functional and cosmetic drawbacks.As an alternative, a gold
weight implant has been used for closure of the upper lid by gravity and, ifnecessary, can be combined with further rehabilitative
facial surgery. A total of 36 patients with peripheral facial paresis were treated with gold weight upper lid implants. Postoperative
closure of the lids was sufficient in all cases. Complications were observed, such as pseudoptosis, deficiency of eyelid closure,
a prominent bulge of the gold implant, in one case frank extrusion of the gold implant, and, surprisingly, in several cases
the formation of a low grade corneal astigmatism. The satisfactory functional results show that the implant is both aesthetically
and functionally superior to tarsorrhaphy.
Received: 4 March 1998 / Accepted: 25 May 1999 相似文献
167.
采用金电极阳极溶出伏安法测定食品中微量的砷,以还原蒸馏法分离干扰离子,在静止溶液中富集As~(3+),此法测定范围可从25—400ppb。用本方法分析水质、饮料、植物油、河产品中微量的砷可得到满意结果。 相似文献
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169.
ABO反定型试剂红细胞的质量控制 总被引:2,自引:0,他引:2
已知血型的试剂红细胞是ABO血型的血清学鉴定中检测抗体必不可少的试剂。为了提高ABO血型检定结果的准确性,解决自制试剂红细胞无标准和无质控的问题,本研究组建立了ABO反定型试剂红细胞的质控方法,进行了试剂红细胞的特异性和亲和性检测、振荡检测、流式细胞术和原子显微镜观察。通过筛选建立了质控参考品,并在此基础上确定质量检定标准及质控方法,同时对制备的3批次试剂红细胞进行了评价。研究结果表明:制备的反定型试剂红细胞既能保证质量和性能的稳定,又能最大限度地降低批次间的差异。结论:制备的AB0反定型试剂红细胞解决了自制红细胞的质控问题,并能提高血型检定结果的准确性。 相似文献
170.
The addition of pyridoxal-5-phosphate for assay of alanine aminotransferase has been recommended. The referral methods are inconvenient for high volume instrumentation due to use of multiple reagents and blanks. We adapted a well-documented method to the centrifugal analyzer as a reference for adaptation of two kit methods. Reference intervals obtained and linearity determined were all similar. The kit methods compared favorably to the referral method. Linear regression analysis yielded the following: DOW = 1.011 BERG + 1.666, r = 0.9965; SKI = 0.938 BERG + 4.559, r = 0.9926; and DSKI = 1.028 BERG + 1.051, r = 0.9889. Precision of the assays was acceptable. We concluded that automation of reagent kits incorporating pyridoxal-5-phosphate is feasible and the assays compared favorably to a recommended method. High volume instrumentation can be used without denigration of analytical quality and to allow comparability to a documented method for interlaboratory review. 相似文献