用Fura-2测定周围血单个核细胞内游离钙浓度

本文用Fura-2测定了周围血单个核细胞内游离钙浓度，并对有关实验条件进行了探讨。对比研究表明，血标本在4℃保存4小时对测定结果无显著影响，测定标本中单个核细胞数以10~5～10~6/ml为宜。认为测定单个核细胞内游离钙浓度对判断单个核细胞的功能状况有一定帮助。     

亚低温配合自由基清除剂治疗脑梗死的临床研究

目的探讨亚低温与自由基清除剂治疗脑梗死的方法和疗效。方法139例脑梗死患者随机分为3组,对照组47例常规治疗;治疗组1:46例采用亚低温治疗;治疗组2:46例给予亚低温配合自由基清除剂治疗。分别评定治疗前后的神经功能缺损评分(NDS)、重要脏器功能指标及预后。结果与对照组相比:亚低温治疗能提高患者治疗有效率(P<0.01),降低NDS(P<0.05)和血乳酸水平(P<0.01),亚低温配合自由基清除剂治疗能显著提高患者治疗有效率(P<0.01),降低NDS(P<0.01)和血乳酸水平(P<0.01),并能显著降低患者肝、肾功能不全和心律失常的发生率(P<0.01 VS对照组);结论亚低温配合自由基清除剂辅助脑梗死的综合治疗可明显改善患者预后、提高患者治疗有效率,降低并发症,值得临床推广。     

芳香烃羟化酶活性与苯并[a]芘中间代谢产物生成关系的研究

100mg香烟烟油,多氯联苯诱导大鼠肝S_9组分中芳香烃羟化酶代谢B[a]P为3-OH-B[a]P的活性分别升高约40,20倍,而代谢B[a] P生成6-OXY-B [a]P自由基产物却仅由多氯联苯诱导的S_9组分作用中才被观察到。     

德国小蠊成虫血淋巴游离氨基酸的测定

采用离心法收集羽化后不同天数的雌雄虫血淋巴，用意大利ＣＡＢＬＯＥＲＢＡ３Ａ３ｏ型氨基酸自动分析仪测定，共检出１０种氨基酸和１个氨残基，其氨基酸总量及每种氨基酸的含量与成虫性别、不同发育期有密切的关系。     

白内障形成机制研究

通过对老年性白内障、外伤性白内障、先天性白内障(共14例)及正常晶体(20例)的SOD测定,表明晶体内自由基产生增加,抗氧化能力障碍是白内障形成的重要原因之一。同时,对晶体内自由基的发生及致病机理进行了探讨,提示在白内障形成初期,给予抗氧化制剂在可能阻止或延缓白内障形成。     

Microvascular transfers of serratus anterior muscles in rats

Summary The serratus anterior muscle in the rat is supplied by the serratus division of thoracodorsal vessels, in a pattern similar to man [2]. The serratus anterior muscles were transferred to the groin area as a free muscle flap in 17 rats and as a free muscle graft in 13 rats. In the free muscle flap group, the serratus anterior muscles of 8 of 10 rats survived; histological examination showed them to be viable and the vascular anastomoses were patent at the 30th day. In the free muscle graft group, all of the muscle grafts became necrotic. As a result, it is concluded that the microvascular transfer of serratus anterior muscles in rats is a useful small animal model for microsurgical laboratory training of clinical free flap transfers and also for performing pharmacological and biochemical studies in transplanted muscle.     

Real time endoscopic imaging of oxyradical generation in pig stomach during ischemia–reperfusion

BACKGROUND: Oxygen-free radicals generation is considered to be a major cause of gastric injury during reperfusion. Chemiluminescence has been used to assess real-time free radical release on the surface of isolated organs. AIMS: To evaluate the combined use of chemiluminescence and gastroendoscopy techniques and to assess the real-time production of free radicals during ischemic damage of the gastric wall in an animal model. PATIENTS AND METHODS: For the experiment, an optical junction was set up between a fibroendoscope and a luminograph apparatus. Three pigs were submitted to gastrofibroendoscopy before, during and after 30 min of clamping of the coeliac artery. Under basal conditions, at the end of the ischemic phase and at the beginning of reperfusion, 1 mM of lucigenin, a specific superoxide enhancer, was injected in the left gastric artery of the animal. The endoscopic live images and chemiluminescence emission were recorded and successively superimposed to measure rate and spatial distribution of photon emission (photons/s). RESULTS: Free radical production was not observed under basal conditions or during the ischemic phase, but significantly increased during reperfusion reaching a maximum peak after 15 min (0.6+/-0.2 photons x 10(5)/s) and decreased progressively thereafter. The superimposition of live and chemiluminescence images allowed the determination of the regional production rate and distribution of photons. CONCLUSIONS: Preliminary observations, in an animal model, on an innovative imaging system which allows the visualization of rate and spatial distribution of reactive oxygen species formation are presented. This new endoscopic technique could be useful for the assessment of oxidative gastric mucosal injury in several gastric diseases; however, further studies remain necessary to determine the applicability of this technique in humans.     

An effective pharyngoesophageal reconstruction with free radial forearm flaps

Summary Treatment of cancer of the cervical aerodigestive tract is challenging due in part to the difficulty in reestablishment of pharyngoesophageal continuity after resection of the involved tract. From May 1989 to August 1990, six patients underwent immediate reconstruction utilizing microvascular transfer of free radial forearm flaps following resection of pharyngoesophageal neoplasms. A small island flap connected to the radial vascular pedicle by fasciocutaneous branch was used to monitor the vascular condition of the hidden fabricated free forearm flap. Stricture is the most troublesome complication of esophageal reconstruction using a conventional free forearm flap. Two small triangular flaps were designed and inserted bilaterally in the distal anastomosis of both lateral esophageal walls to prevent circular contracture. The outer layer sutures were anchored to surrounding rigid structures to withstand shrinkage and circular contraction. The problem of stricture was solved by these procedures. This one-stage, easily monitored operation for pharyngoesophageal reconstruction is considered to be as useful as a free jejunal transfer.     

Free flap reconstruction of the lower extremity in the elderly

Summary Over a four year period, thirteen patients over the age of 60 (mean 69 years) underwent reconstruction of lower extremity defects with free tissue transfers. Ten latissimus dorsi, one tensor facia lata, and two rectus abdominus free flaps were used. Despite significant risk factors which included previous tobacco use, hypertension, diabetes mellitus, and angiographic vessel abnormalities, ten of thirteen cases had successful outcomes. There was one acute flap failure secondary to arterial thrombosis. Four flaps had delayed healing, with one patient eventually requiring amputation secondary to persistent pain and wound drainage. This experience demonstrates that free flap reconstruction of lower extremity defects in the elderly patient is a reasonable alternative to amputation when other options are limited.From the Section of Plastic and Reconstructive Surgery at the Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA Requests for reprints: J. Fisher, M.D.