肺癌患者血清铜和铁蛋白测定分析

 目的: 探讨癌症病人血清铜和血清铁蛋白之间的相互关系, 方法: 分别用原子吸收分光度计火焰法和放射免疫分析法测定了27例肺癌和45名正常人的血清铜和血清铁蛋白水平并作统计学分析。结果: 肺癌组的血清铜水平和铁蛋白水平均比对照组增加而且均有非常显著意义(P<0.01)。相关关系分析结果显示：人体血清铜与血清铁蛋白两者相互有高度的正相关关系(P<0.005)。鉴于血清铜绝大部分是以本质为亚铁氧化酶的铜蓝蛋白形式存在, 能促进铁的吸收, 而铁蛋白是机体对铁起贮存作用的蛋白质, 结论: 肺癌患者血清铁蛋白水平异常升高是铜蓝蛋白水平增高所致。     

Ferritin is a component of the neuritic (senile) plaque in Alzheimer dementia

Summary A strong immunoreactivity for ferritin was observed in the neuritic (senile) plaques in Alzheimer's disease hippocampus. The ferritin accumulation was almost exclusively associated with the microglia, which appeared to have proliferated greatly. These cells were also positive for HLA-DR, a putative marker for reactive microglia. In contrast, in the diffuse plaques, which were without neuritic pathology, the ferritin-stained microglia appeared to be normal. Microglia were seen frequently in contact with neurons undergoing neurofibrillary changes but only the tangles in the extracellular space were ferritin positive. No ferritin was detected, by Western blots, in paired helical filaments isolated from Alzheimer's disease brain, suggesting that ferritin was most likely weakly associated with and was not a constituent of these fibrils. No correlation between increased ferritin/microglia activity and blood-brain barrier leakage was detected. Ferritin, an iron-storage protein, might have a role in the formation of amyloid through the action of free radicals generated during the release of iron from the ferritin molecule. Alternatively, the ferritin/microglia system might be secondarily involved in the removal and processing of the amyloid.Supported in part by the New York State Office of Mental Retardation and Developmental Disabilities and National Institutes of Health grants NS18105, AG05892 and AG04220. H. L. was funded by a grant from the Ministry for Science and Research, Austria, J. G. J. and J. F. were funded by the Council for Tobacco Research. Parts of this paper have been reported at the 9th International Conference on Proteins of Iron Transport and Storage, Brisbane, Australia, June 1989 and at the 2nd International Conference on Aluminium and Health, Orlando, Fla, USA, December 1989     

Untersuchungen zur elektronenmikroskopischen Darstellung der Kapsel von Cryptococcus neoformans. Ultrastructural Research on the Capsule of Cryptococcus neoformans

Zusammenfassung: An drei Cryptococcus-neoformans-Stämmen wurden elektronenmikroskopische Untersuchungen zur Darstellung ihrer Kapsel durchgeführt. Mittels polykationischem Ferritin gelang die kontrastreiche Markierung der Polysaccharidkapsel auf der Basis einer zytochemisch-physikalischen Reaktion zwischen den negativen Ladungsträgern der Extrazellularschicht und dem kationischen Marker. Zusätzlich konnten extrazellulär fibrilläre Strukturen differenziert werden, an die eine Anlagerung von Ferritin nicht erfolgt war. Geringfügige methodische Variationen verringerten die Ferritinmarkierung erheblich, eine einstündige Hitzebehandlung hob sie ganz auf. Dagegen traten unmarkierte Bestandteile verstärkt in den Vordergrund. Bei dem Versuch einer Interpretation dieser elektronenoptischen Befunde wurde die Möglichkeit diskutiert, daß die Kapsel von Cryptococcus neoformans einen von dem leicht ablösbaren Polysaccharid zu unterscheidenden Anteil mit fibrillärem Aufbau enthält, der evtl. im Sinne eines Stützgerüstes für die Ein- und Anlagerung des löslichen Kapsel-Antigens fungiert. Summary: Ultrastructural research on the capsule of Cryptococcus neoformans. For ultrastructural visualization of their capsule, three Cryptococcus neoformans-strains were treated cytochemically with a polycationized derivative of ferritin. This marker binds electrostatically to the anionic sites of polysaccharide capsules. After treatment, the yeast cells were covered with a dense irregular layer of ferritin granules. Moreover, fibrillary components could be detected extracellularly, which did not bind cationic ferritin. Slight methodical variations, e. g. pretreatment of the yeasts by heat, led to an intensification of these structures, whereas binding of ferritin decreased or was abolished, respectively. These findings indicate that the capsule of Cryptococcus neoformans apparently consists of two different components: one seems to bind cationic ferritin, is easily removable, and equivalent to the soluble polysaccharide antigen found in serum and liquor during Cryptococcus-infection, and a second fibrillary one is possibly acting as a supporting framework for the soluble component.     

A cross sectional study of maternal iron status of Korean women during pregnancy

The purpose of this study was to determine the iron status of Korean women during pregnancy and to assess the relationship between maternal iron status and the outcome of their newborns. A total of eighty-one pregnant women living in Gwangju, Korea, participated in the study: 26 women were in the first trimester, 23 in the second trimester, and 32 in the third trimester. Maternal red blood cell (RBC) number, hemoglobin (Hb) concentration, and serum iron and ferritin levels were reduced significantly in the last trimester (p < 0.05) compared to the findings both in the first and second trimesters. On the other hand, total iron binding capacity (TIBC), transferrin level, and the ratio of sTfR to ferritin in the third trimester were higher (p < 0.05) than those both in the first and second trimesters. Dietary intake of iron in the three trimesters was 9.7 ± 2.3, 13.3 ± 4.3, and 10.6 ± 2.5 mg/day, respectively. All were far below the Korean Recommended Dietary Allowances (RDA) of iron for pregnant women. Approximately, ninety percent of the subjects consumed iron supplements after the 20th week of their pregnancies until delivery. The supplemental iron intake in the second and third trimesters was 40 ± 12 and 46 ± 11 mg/day, respectively. There was a significant correlation between the maternal Hb level in the third trimester and the birth weight of infants. In conclusion, maternal iron status deteriorated during pregnancy, although most subjects consumed more than the RDA of iron by taking iron supplements after the 20th week of pregnancy. The results confirm that maternal iron deficiency during pregnancy negatively affects the outcome of newborns.     

献血员献血时间对血清铁蛋白、转铁蛋白的影响

目的：探讨献血员献血时间对血清铁蛋白（SF），转铁蛋白（TF）含量的影响，方法：分别应用放免法和免疫比浊法对138名献血员进行了SF和TF含量测定，并与345名正常人作对照，结果：女献血员SF含量低于正常（P＜0．05），而TF含量则高于正常（P＜0．05），献血时间越长，差异越显（P＜0．01），结论：测定SF和TF含量对女献血员的缺铁状态有一定的诊断价值。     

Hepcidin-to-ferritin ratio: A potential novel index to predict iron overload-liver fibrosis in ß-thalassemia major

ObjectivesWe aimed to determine a threshold cutoff for hepcidin, ferritin, and the hepcidin-to-ferritin ratio in the diagnosis of liver fibrosis caused by iron overload in chronic hepatitis C virus (HCV)-free ß-thalassemia major patients .MethodsThis 1:1-matched case-control study included 102 individuals (3–30 yr.); 51 ß-thalassemia major patients with iron overload , and 51 apparently healthy individuals.ResultsThe highest areas under the receiver operating characteristic curves (AUC-ROCs) for the diagnosis of patients vs. controls had overlapping 95% confidence intervals (CIs): serum hepcidin (0.758; 0.64–0.87;    P ? 0.001), serum ferritin (1.000; 1.00–1.00; P  ?0.001), and the hepcidin/ferritin ratio (1.000; 1.00–1.00; P ? 0.001). For differentiation of patients with liver fibrosis stages of F0–F1 vs. F2–F4 and F0–F1 vs. F3–F4, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) with P-values ? 0.001 were the only statistically significant parameters, while the AUC-ROCs of the hepcidin/ferritin ratio (0.631, P = 0.188 and 0.684, P = 0.098) exhibited 90% and 89.5% sensitivity, respectively, in staging liver fibrosis.ConclusionOur results showed that the hepcidin/ferritin ratio is as effective as the APRI and maybe a better predictor for the diagnosis of liver fibrosis and discriminating its stages, with excellent sensitivity and specificity compared to its components.     

肿瘤标志物三项联合检测在鼻咽癌诊断中的意义探讨

目的探讨血清EB病毒(EBV)、铁蛋白(SF)及细胞角蛋白19片段(CYFRA21-1)对鼻咽癌(NPC)的临床诊断价值。方法采用免疫酶法及电化学发光免疫分析等技术,检测184例NPC患者、52例鼻咽良性疾病患者、80名正常对照者血清中的EBV壳抗原免疫球蛋白A抗体(EB-VCA-IgA)、SF及CYFRA21-1表达水平。结果NPC组EB-VCA-IgA、SF、CYFRA21-1敏感性分别为79.3%、46.7%、42.4%。3项联合检测敏感性为92.4%,与单项检测比较差异有显著性(P<0.05)。结论EB-VCA-IgA、SF、CYFRA21-1联合检测对NPC的诊断具有较高的临床价值。     

肿瘤标志物谱对常见肿瘤的筛查意义

目的 探讨用简单的肿瘤标志语来筛查提高常见肿瘤的检出率。方法 将甲胎蛋白(AFP)、癌胚抗原(CEA)、铁蛋白(Ft)、唾液酸(SA)组合为简单的肿瘤标志物谱——‘癌谱’,用定量ELISA法检测‘癌谱’中的AFP、CEA、Ft,用化学法检测SA。将临床确诊的各类常见肿瘤病人,良性疾病人群及健康体检人群作“癌谱”测定,结果经统计分析作肿瘤诊断的阳性率、特异性、阳性预测值、阴性预测值评价。结果 ‘癌谱’对肿瘤组的阳性率为89．7％,特异性90．3％。阳性预测值90．1％,阴性预测值89．9％,常见肿瘤的灵敏度分别为：肝癌95．6％、肺癌(腺癌和鳞癌)92．1％、胃癌86．6％、直结肠癌81．6％、乳腺癌84．2％、绒癌93．8％、卵巢癌88％。结论“癌谱”是一种筛查常见肿瘤的简单、廉价的实验室方法。     

血清铁蛋白水平与冠状动脉病变程度的相关性探讨

目的 现察血清铁、铁蛋白及转铁蛋白与冠状动脉狭窄的关系.探寻冠心痛的发病原因.方法 对315例冠心病患者行冠状动脉造影和血清铁、铁蛋白及转铁蛋白的测定,采用Gensini评分系统衡量冠状动脉造影结果 .按照Gensini评分的三分位间距将观察时象分为3组,应用方差分析研究铁代谢指标在3组中的分布情况,应用多因素回归分析研究Gensini评分与铁代谢指标的关系.结果 单因素方差分析显示.血清铁、血清铁蛋白及血清转铁蛋白/血清铁蛋白比值与Gensini评分肴相关性(相关系数分别为6.162、7.191和4.380,均P<0.05);多因素逐步线性回归分析显示,血清铁蛋白与冠状动脉狭窄程度的评分独立相关(其偏相关系数为0.240,P<0.01).结论 血清铁蛋白与冠状动脉狭窄独立相关.     

Relation between iron content of serum ferritin and clinical status factors extracted by factor analysis in patients with hyperferritinemia

OBJECTIVES: The aims of this study were to develop a new technique for determination of iron content of serum ferritin (ICF, micromol Fe/mg protein) and to investigate relations between ICF and clinical status in patients with hyperferritinemia. METHODS: ICF values were determined by a combination of immunoprecipitation of ferritin and direct colorimetric iron assay. One hundred fifty patients with hyperferritinemia were screened. Factor analysis of the results of 11 laboratory tests was applied to extract factors representing the clinical status of patients. Relations between the extracted factors and the ICF values or serum ferritin concentrations were assessed. RESULTS: Within-run coefficients of variation (CVs) of the ICF assay were <==5.7%. The mean ICF value of 150 patients was 0.423 micromol/mg (SD, 0.211 micromol/mg). Three factors representing clinical status were identified: inflammation, tissue cell damage, and body iron status. Serum ferritin level correlated with all three factors. In contrast, ICF correlated significantly only with the factor representing tissue cell damage (r = 0.293, p = 0.001), and this correlation was independent of inflammation and iron status (p = 0.008). CONCLUSIONS: ICF changes in response to tissue cell damage independent of inflammatory and body iron statuses, whereas serum ferritin changes in response to all three pathologic statuses.