Biocompatibility and Adhesion Formation of Different Endoloop Ligatures in Securing the Base of the Appendix

Background and Objectives:

The common technique used in securing the base of the appendix is Endoloop ligature (Ethicon, Somerville, NJ, USA). Vicryl (polyglactin 910) (Ethicon) and polydioxanone (PDS) (Ethicon) Endoloop ligatures can be used. There are potential benefits of the use of PDS Plus (Ethicon) Endoloop ligature. However, the use of different materials may vary in terms of inflammation, foreign-body reaction, rate of infection in the surgical area, or rate of adhesion formation. An ideal suture would induce minimal inflammatory response and adhesion formation.

Methods:

Ninety rats were randomized into 3 groups: group I, in which appendectomy was performed with Vicryl ligature; group II, in which appendectomy was performed with PDS ligature; and group III, in which appendectomy was performed with PDS Plus ligature. The animals were killed on the seventh, 28th, and 60th days after surgery. The secured stump was used for histopathologic and immunohistochemistry analysis, as well as evaluation of the formation of adhesions.

Results:

Mild and moderate inflammation was more frequent in the PDS and PDS Plus groups than in the Vicryl group on the seventh postoperative day. There were no significant differences in the degree of inflammation on the 28th and 60th postoperative days. The lowest degree of postoperative adhesions was observed in the PDS group.

Conclusion:

Milder postoperative inflammatory changes and a lower degree of postoperative adhesions were seen in the PDS ligature group, suggesting that this could be the standard Endoloop used to secure the base of the appendix.     

内镜下尼龙绳联合高频电凝切除术治疗结肠巨大息肉临床应用

目的探讨内镜下尼龙绳套扎联合高频电凝切除术的方法治疗结肠巨大息肉,并评价其安全性和疗效。方法通过内镜检查确诊的30例结肠直径〉2cm的息肉（共30枚）,先采取用尼龙绳圈经内镜套扎于息肉的根部,再采取内镜下高频电凝切除术切除,2周后复查内镜。结果本组30例共30枚息肉均套扎成功,并同时进行高频电凝切除术,术后2周复查27例27枚息肉完全脱落消失,3例3枚息肉有蒂端残留,以氩束凝切除,2个月后复查创面修复。本组治疗的治愈率、有效率、无效率分别为90％、10％、0％。所有患者均未见出血、穿孔等并发症。结论内镜下尼龙绳套扎联合高频电凝电切除术治疗结肠巨大息肉的方法安全有效,并发症少。     

腹腔镜阑尾切除术阑尾根部3种处理方法的比较

目的比较腹腔镜阑尾切除手术中分别应用3种不同方法处理阑尾根部：丝线结扎、塑料夹（Hem—O—lok）夹闭、圈套器（Endoloop）套扎的效果。方法2010年2月～2011年8月168例因急性阑尾炎接受急诊腹腔镜阑尾切除术,采用3种不同方法处理根部：阑尾根部用丝线结扎50例（丝线组）,用Hem—O—lok夹闭65例（Hem—O—lok组）,用Endoloop套扎53例（Endoloop组）,比较3组术中、术后情况。结果3组术后并发症、术后住院时间无显著性差异（P〉0．05）。丝线组手术时间（54．7±5．6）min,显著长于Hem—O-lok组（44．7±5．5）min（q=13．903,P〈0．05）和Endoloop组（49．1±5．1）min（q=7．428,P〈0．05）。丝线组手术费用（3285．2±55．4）元,显著多于Hem．O—lok组（3083．3±80．0）元（q=23．402,P〈0．05）,但显著少于Endoloop组（3646．5±50．8）元（q=39．957,P〈0．05）。结论3种处理阑尾根部的方法都是安全、有效的,但Hem—O—lok夹闭比丝线结扎和Endoloop套扎,更省手术时间和手术费用,并且更适合于初学者。     

Percutaneous transgastric endoscopic tube ileostomy in a porcine survival model

AIM To introduce natural orifice transgastric endoscopic surgery(NOTES) tube ileostomy using pelvis-directed submucosal tunneling endoscopic gastrostomy and endoscopic tube ileostomy.METHODS Six live pigs(three each in the non-survival and survival groups) were used. A double-channeled therapeutic endoscope was introduced perorally into the stomach. A gastrostomy was made using a 2-cmtransversal mucosal incision following the creation of a 5-cm longitudinal pelvis-directed submucosal tunnel. The pneumoperitoneum was established via the endoscope. In the initial three operations of the series, a laparoscope was transumbilically inserted for guiding the tunnel direction, intraperitoneal spatial orientation and distal ileum identification. Endoscopic tube ileostomy was conducted by adopting an introducer method and using a Percutaneous Endoscopic Gastrostomy Catheter Kit equipped with the Loop Fixture. The distal tip of the 15 Fr catheter was placed toward the proximal limb of the ileum to optimize intestinal content drainage. Finally, the tunnel entrance of the gastrostomy was closed using nylon endoloops with the aid of a twin grasper. The gross and histopathological integrity of gastrostomy closure and the abdominal wall-ileum stoma tract formation were assessed 1 wk after the operation.RESULTS Transgastric endoscopic tube ileostomy was successful in all six pigs, without major bleeding. The mean operating time was 71 min(range: 60-110 min). There were no intraoperative complications or hemodynamic instability. The post-mortem, which was conducted 1-wk postoperatively, showed complete healing of the gastrostomy and adequate stoma tract formation of ileostomy.CONCLUSION Transgastric endoscopic tube ileostomy is technically feasible and reproducible in an animal model, and this technique is worthy of further improvement.     

内镜下钛夹、尼龙圈套辅助高频电切术治疗胃肠道巨大有蒂息肉的临床研究

目的 评价内镜下钛夹、尼龙圈套辅助高频电切术治疗胃肠道巨大息肉的效果及安全性.方法 对50例患者共85处消化道巨大有蒂息肉样病变分别采用钛夹、尼龙圈套辅助高频电凝切除术的治疗方法进行回顾性分析.结果 85枚巨大息肉中,上消化道15枚,结肠66枚,直肠4枚.其中62枚息肉采用尼龙圈套＋高频电凝切除术治疗,23枚息肉采用高频电凝切除术＋肽夹钳夹术治疗,术中无一例发生出血;术后迟发性出血2例（2.4%）,急诊内镜下钛夹止血成功,无其它并发症发生.结论 消化道息肉高频电切术后最常见且最棘手的并发症是出血,巨大息肉发生在术中或术后迟发性出血的机率更大,采用尼龙圈套及金属肽夹辅助高频电凝切除术,能有效预防粗蒂大息肉样病变切除术中和术后出血,是消化道巨大息肉内镜下治疗安全有效的联合治疗技术.     

金属夹联合尼龙套圈在内镜带蒂大息肉摘除中的应用

背景：消化道出血是内镜息肉摘除术最常见的并发症。目的：观察金属夹联合尼龙套圈对内镜带蒂大息肉摘除术的效果和安全性。方法：选取2011年6月一2012年8月上海市第一人民医院分院89例带蒂大息肉患者,分为尼龙套圈组（A组）和金属夹联合尼龙圈套组（B组）,回顾性分析息肉形态、部位、大小以及并发症发生率。结果：A组和B组息肉形态均以山田Ⅳ型为主,主要位于乙状结肠,两组患者性别、年龄、息肉顶部、蒂部平均直径相比均无明显差异（P〉0．05）。A组和B组的术中出血发生率（12．8％对10．0％）、术后迟发性出血发生率（10．3％对4．0％）相比均无明显差异（P〉0．05）,经治疗后出血均停止。A组2例患者术后3个月息肉复发,B组手术创面愈合良好,无息肉残端残留。结论：金属夹联合尼龙套圈能有效预防内镜下带蒂大息肉切除术中和术后的出血。     

腹腔镜圈套器辅助钛夹处理阑尾根部的研究

目的:研究腹腔镜下应用圈套器套扎辅助钛夹加固法处理阑尾根部的可靠性与安全性。方法:418例患者应用圈套器套扎辅助钛夹加固法处理阑尾根部,常规腹腔镜下切除阑尾。结果:418例患者无粪漏及其它术后并发症发生,平均住院2.8d。结论:腹腔镜阑尾切除术采用圈套器套扎辅助钛夹加固法处理阑尾根部安全可靠,操作简便,经济实用。     

内镜全层切除术后胃壁缺损的金属夹联合尼龙绳间断缝合术

目的探讨金属夹联合尼龙绳间断缝合术修补内镜下全层切除术（EFR）治疗后胃壁缺损的临床价值。方法回顾性分析2011年3—10月间复旦大学附属中山医院内镜中心行金属夹联合尼龙绳间断缝合术修补EFR治疗后胃壁缺损患者的临床资料,评价其可行性、安全性和疗效。结果共计14例患者．中位年龄49（32-63）岁。病变位于胃体5例,胃底8例,胃窦与胃体交界l例。病变大小为0．6～3．0cm。应用金属夹联合尼龙绳间断缝合术修补成功率100％（14／14）,内镜下修补中位用时12（8-201min,无中转外科手术或腹腔镜手术病例。4例于治疗后1d出现腹部胀痛或隐痛．体温升高．其中2例经B超提示腹腔有少量积液,给予穿刺引流;4例均继续半卧位,持续胃肠减压和抗感染治疗1～3d后好转出院。术后14例均获随访,中位随访时间3（1～8）个月,2例创面金属夹和尼龙绳残留．给予内镜下异物钳取出,创面愈合好。结论金属夹联合尼龙绳间断缝合术修补EFR治疗后胃壁缺损安全而有效,可以成为临床上消化道缺损内镜下修复的方法之一。