应用酶消化法使囊虫循环抗原游离的研究

检测囊虫病循环抗原(CA)时,预先用胰酶或木瓜酶消化可以使血清免疫复合物(1C)中的CA完全游离。用3.5％聚乙二醇(PEG)沉淀血清中的1G,再以胰酶消化,在抗体过量时CA的得率可达100％。应用ELISA双抗体夹心法检测自抗体过量型至抗原过量型的系列1C模拟血清,仅抗原过量时呈阳性;囊虫病人血清阳性率为43.1％(25/58),用胰酶消化后检测,由阴性变为阳性者占22.58％(7/31)。建议用PEG分离浓缩血清中的1C,再以胰酶或木瓜酶消化,以提高血清CA阳性率。     

囊尾蚴病发病过程中的IgE及其介导的免疫反应

检测了35例囊尾蚴病患者和30例献血员血清中总IgE水平、特异性IgE和组织胺含量,并进行了间接特异性肥大细胞脱颗粒试验。患者血清中总IgE水平达730.6IU/ml,而正常人的总IgE水平仅为186.5IU/ml。患者血清中特异性IgE的阳性率为85.7％,间接特异性肥大细胞脱颗粒的阳性率和血清中组织胺含量均明显高于正常人。     

Host response in childhood neurocysticercosis

The autopsy findings in 18 cases of childhood neurocysticercosis are described. Twelve of the children had died from other causes. Asymptomatic disease correlated with immunodeficiency and with negative serum anticysticercosis antibody test.     

脑囊虫病的CT诊断(附110例分析)

分析了110例各型脑囊虫病的CT表现、各型CT表现的病理基础,提出将CT表现结合囊虫发育的病理演变过程进行分期,以利动态观察与治疗。认为单个不典型病灶易误诊,应特别注意,并观察了24例经口服吡喹酮治疗半年后的疗效。     

脑囊虫病的CT诊断(附600例)

目的：探讨ＣＴ对脑囊虫病的诊断价值。方法：６００例中，男３７５例，女２２５例，发病年龄２～７７岁。根据病史、临床及ＣＴ表现，囊虫试验阳性及治疗随访观察证实者５４０例，手术与病理证实６０例。结果：脑实质型５０８例，脑室及脑膜下型１０例，脑膜脑池型１３例，混合型６９例。主要ＣＴ表现（１）小粟粒点状，小囊状及小囊内点状病灶；（２）点状、结节状、环状及环内点状强化灶；（３）其它：大环状、大囊状病灶及脑积水等。结论：小粟粒点状、小囊状及小囊内点状病灶或点状、结节状、环状及环内点状强化灶，是诊断脑囊虫病重要依据。     

Immunochemical detection of antigens of larval Taenia solium and anti-larval antibodies in the cerebrospinal fluid of patients with neurocysticercosis

A simple and quantitative enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of antigens of larval Taenia solium in the cerebrospinal fluid (CSF) of patients with cerebral cysticercosis. Another ELISA was developed for detecting antibodies in CSF against larval antigens. The examination of sixteen patients with clinical diagnosis of cerebral cysticercosis revealed that eleven patients had both circulating larval antigens and anti-larval IgG (but not IgM) antibodies in their cerebrospinal fluids. Of these patients, those with surgically and histologically confirmed infections were all positive by the two tests. CSF samples from nine normal individuals and from six patients suffering from proven neurological disorders other than neurocysticercosis were negative for both tests. In development of these assays it was found that cross-linking of antigens to microtiter plates further improved the performance of the ELISA. The results of this study suggest that either or both of these tests may be useful in discriminating between neurocysticerosis and other clinically related diseases.     

寄生虫病DNA疫苗研究进展

寄生虫病防治策略之一是研制安全有效的疫苗,DNA疫苗是近10多年发展起来的新型疫苗。近年来寄生虫病DNA疫苗研究取得了很大进展。本文就DNA疫苗的免疫机理、构建与优化、佐剂、递送途径,以及疟疾、血吸虫病、囊尾蚴病、弓形虫病等重要寄生虫病DNA疫苗的研究进展作一综述。