Short regimen of praziquantel in the treatment of single brain enhancing lesions

Twenty-six patients with single enhancing brain lesion (SEL) were openly assigned to receive single-day praziquantel therapy (n=14), or not (n=12). From 14 treated patients, complete resolution was found in 11, partial resolution in two, and the remaining case was later diagnosed as an arteriovenous malformation. Side effects presented in only one patient and remitted in the same day with symptomatic treatment. Conversely, the lesions persisted unchanged in six of 12 patients in the non-treatment group. Untreated patients with persisting lesions were prescribed praziquantel treatment. After this, SELs disappeared in three cases, other diagnoses (brain tuberculoma and arteriovenous malformation) were made in two, and one was not evaluated. When analyzed in regard to the baseline serology, resolution of lesions on computed tomography was found in 13 (complete=12, partial=1) of 14 seropositive patients, whereas it only happened in six (complete=5, partial=1) of 12 seronegative patients. Serological screening defines a subset of SEL patients with good prognosis. If antiparasitic therapy is to be used in patients with SEL, and we cannot find a strong argument against it, single-day praziquantel is the regimen of choice based on duration, costs, and minimal side effects.     

A Case of Neurocysticercosis in Entire Spinal Level

Cysticercosis is the most common parasitic infection affecting the central nervous system. Spinal neurocysticercosis (NCC) is very rare compared with intracranial NCC and requires more aggressive management because these lesions are poorly tolerated. The authors report a case of intradural extramedullary cysticercosis of the entire level of spine with review of the literature.     

4种CNS传染性疾病脑脊液嗜碱性粒细胞对比观察:192例

应用脑脊液细胞玻片离心法,对192例经住院临床确诊的4种中枢神经系统(CNS)传染性疾病住院病人脑脊液嗜碱性粒细胞(basos)进行了检查,并将4个亚组之阳性率进行比较,卡方检验表明,脑猪囊虫病与结核性脑膜炎/化脓性脑膜炎/病毒性脑膜炎之间有极显著差异(P<0.001)。作者等还指出,脑脊液中 basos 阳性率增高与嗜酸性粒细胞、浆细胞阳性率增高之同时存在,对脑猪囊虫病的诊断及其与置面的3种脑膜炎之鉴别诊断有重要参考价值。     

An Incidental Diagnosis of Neurocysticercosis in a Dental Patient

Tenia solium, a parasite causes cysticercous cellulose when affecting the central nervous system, the manifestation is called neurocysticercosis. The most common symptom in neurocysticercosis is seizure. Generally, oral diagnosticians come across cases of oral cysticercosis and it is rare to find a case of neurocysticercosis in the dental office, as it goes undetected. Sometimes, when patients experience seizure in the dental office and subsequent evaluation is performed, rarity such as this can be detected. One case of neurocysticercosis in a 27 year old unmarried female patient detected due to its presentation in the dental office is being reported here.     

鲁北地区绦／囊虫病流行因素调查分析

目的 调查分析鲁北地区绦虫病与囊虫病的流行情况。方法 采用现场访问、粪便检查及血清学检测等方法对鲁北4市的5县区15个乡镇43个行政村的31124人进行了调查。结果 人群带绦虫病患病率3．2／万，囊虫病患病率5．1／万，有较明显的职业分布特征。人群血清猪囊尾蚴特异性IgG4抗体阳性率1．38％，商品猪血清囊尾蚴抗体阳性率0．38％。结论 初步搞清了山东省重点疫区带绦虫病与囊虫病的流行现状，并对该病的各流行环节做了分析，为该病的预防提供了参考信息。     

快速斑点免疫金渗滤法检测血清中囊虫抗体的研究

为建立一种快速敏感、适于基层应用的检测囊虫抗体的方法,将囊尾蚴抗原点于硝酸纤维素膜(NC)上,用来检测血清中囊虫抗体,通过胶体金标记的金黄色萄萄球菌A蛋白(SPA)直接显色,阳性者出现红色斑点,结果易于判断.结果表明,整个试验过程仅需5分钟,操作简单,用该法与酶联免疫吸附试验对比检测57份囊虫患者血清标本,2法均阳性为54份,2法均阴性为1份,总符合率为95%.结果提示斑点免疫金渗滤法简便、快速、适于基层应用,可用于囊虫病的诊断及流行病学调查.     

融合蛋白抗原检测不同病程及不同类型囊虫病

猪囊尾蚴四个阳性克隆：２８ＫＤａ、１８ＫＤａ、１４ＫＤａ、３４ＫＤａ以等比联合使用，经异丙基硫代－β－Ｄ半乳糖苷（ＩＰＴＧ）诱导出多克隆β－半乳糖苷酶—猪囊尾蚴重组基因融合蛋白（ＦＰ）抗原，以简化—Ｗｅｓｔｅｒｎｂｌｏｔ（ＳＷＢ）法检测不同病程及不同类型囊虫病，并与粗制抗原（ＣＡ）进行的ＥＬＩＳＡ、ＩＨＡ法相对比。结果显示：用多克隆ＦＰ—ＳＷＢ可提高检出率，且识别抗体更灵敏，阳性率＞８０％，囊虫病人血清循环抗体至少３年中仍有相当高的检出率（＞６０％）。     

IHA法间接检测囊虫病患者血清特异抗原的探讨

目的 探讨适用于基层医院检测囊虫抗原的方法。方法 囊液抗原致敏血球间接血凝（IHA）法，检测待检血清与含1：8＋囊虫抗体兔血清的混合液的抗体，低于1：2#为阳性。结果 检测未经治疗的囊虫患52例和用吡喹酮治疗后的患41例，阳性率分别为82.69%和85.37%。结论 囊液抗原致敏血球IHA法间接检测囊虫患血清特异抗原结果稳定，方法简便，临床符合率较高。     

Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot

Objective

A commercial cysticercosis Western blot was evaluated for serological cross-reactivity of sera from patients with alveolar (AE) and cystic echinococcosis (CE).

Methods

A total of 161 sera were examined, including 31 sera from AE-patients, 11 sera from CE-patients, 9 sera from patients with other parasitic diseases and 109 sera from patients with unrelated medical conditions. All AE-and CE-sera were also examined by the echinococcosis Western blot.

Results

More sera from patients with AE than with CE showed cross-reactivity in the form of ladder-like patterns ("Mikado aspect") and untypical bands at 6-8 kDa (71% and 77.4% versus 27.3% and 45.5%, respectively). In contrast, triplets of bands in the area above 50 kDa and between 24 and 39-42 kDa were more frequent in CE than in AE sera. The fuzzy band at 50-55 kDa typical for cysticercosis was absent in all AE and CE sera.

Conclusions

Atypical banding patterns in the cysticercosis Western blot should raise the suspicion of a metacestode infection different from Taenia solium, i.e. Echinococcus multilocularis or E. granulosus, especially when the Mikado aspect and an altered 6-8 kDa band is visible in the absence of a fuzzy 50-55 kDa band.     

猪囊尾蚴病患者CD4~+ CD25~+调节性T细胞水平的变化

目的检测猪囊尾蚴病患者外周血中CD4+CD25+调节性T淋巴细胞及其FOXP3的表达情况,探讨CD4+CD25+调节性T淋巴细胞在猪囊尾蚴感染中的免疫调控作用及意义。方法采用流式细胞仪检测11例猪囊尾蚴病患者外周血中CD4+CD25+T淋巴细胞的含量,同时观察CD4+CD25+T细胞中表达FOXP3群体的百分含量。结果囊尾蚴病患者外周血中CD4+CD25+T淋巴细胞的百分含量为6.11%,较正常人(3.94%)明显升高(P<0.05);患者外周血中CD4+CD25+T细胞表达FOXP3的细胞百分含量为15.67%,与正常对照组(11.09%)有显著性差异(P<0.05)。结论猪囊尾蚴病患者外周血中CD4+CD25+T淋巴细胞的百分含量显著升高,表明CD4+CD25+调节性T细胞可能参与猪囊尾蚴感染的免疫抑制。