Human chronic kidney allograft rejection is accompanied by increased intraglomerular cathepsin B and L activity

Abstract The major reason for late graft losses is chronic rejection. Recently, a large number of studies have indicated that proteolytic enzymes play an important role as mediators of glomerular injury. The cysteine proteinases cathepsins B and L degrade structural matrix proteins such as type I collagen and laminin. We investigated intraglomerular protease activities in 12 patients after kidney graftectomy because of end-tage renal disease following chronic rejection. A group of 12 patients undergoing nephrectomy because of cancer served as controls using only non-involved parts of the kidney. The activities of cathepsins B and L in homogenates of isolated glomeruli were measured fluorometrically methylcoumarylamidc substrates and related to DNA content. In rejected kidney allografts we observed significantly enhanced intraglomerular cathepsin B activity and cathepsin B + L activity.     

维生素K对HBsAg与抗HBS结合的抑制作用

在ＲＰＨＩ试验中，将ＶｉｔＫ１或ＶｉｔＫ３预先与ＨＢｓＡｇ阳性血清作用后，再加抗ＨＢｓ单克隆抗体诊断红细胞，可见红细胞呈圆点状沉淀，提示ＨＢＳＡｇ与抗ＨＢｓ的结合受到抑制，该抑制作用呈浓度依赖性．ＲＰＨＩ试验后的抗ＨＢｓ单克隆抗体诊断红细胞，经洗涤后再与ＨＢｓＡｇ阳性血清作用，仍出现明显凝集，表明ＶｉｔＫ对ＨＢｓＡｇ与抗ＨＢｓ结合的抑制作用不是通过影响抗ＨＢｓ而产生的。     

Paraneoplastic movement disorder in a patient with non-Hodgkin's lymphoma and CRMP-5 autoantibody.

The paraneoplastic autoantibody, collapsin response-mediator protein (CRMP)-5 immunoglobulin G (IgG), is specific for neuronal cytoplasmic CRMP-5, and is usually associated with small-cell lung carcinoma or thymoma. We report on details of a movement disorder that followed anti-B-cell therapy in a patient with lymphoma, and was accompanied by CRMP-5 IgG.     

慢性乙型肝炎及乙肝后肝硬化患者血清中自身抗体结果分析

目的 :探讨慢性乙型肝炎及乙肝后肝硬化患者血清中自身抗体的变化情况。方法 :收集了慢性乙型肝炎患者 2 4 5例 ,乙肝后肝硬化 86例血清标本测定抗核抗体 (ANA)和可提取性核抗原 (ENA)抗体。结果 :慢性乙型肝炎和乙肝后肝硬化ANA的检出率分别为 13.1% (31/ 2 4 5 )、2 3.2 % (2 0 / 86 ) ,均明显高于正常对照组的 2 .5 % (2 / 80 ) (P <0 .0 5 )。两组病例间也有显著性差异 (P <0 .0 5 )。抗ENA抗体阳性率分别为 7.34%和 12 .7%。阳性主要有nRNP/Sm、SS -A、Scl- 70、Jo - 1、dsDNA等多种类型。结论 :慢性乙型肝炎及乙肝后肝硬化患者血清自身抗体显著高于正常人。除一部分是目前已鉴定的与常见自身免疫性疾病相关的自身抗体外 ,还有许多有待进一步鉴定。     

单项抗HBc阳性的临床分析

临床上对于乙型肝炎的诊断主要是依据病人的 HBV- M结果进行判断 ,对于单项抗 HBc阳性的临床意义 ,目前有不同的争议。通过在临床工作中搜集整理的有关资料进行总结分析 ,认为单项抗 HBc阳性的意义与其在血清中的滴度有关。     

小牛胸腺肽治疗乙型肝炎的疗效研究

本文报告了小牛胸腺肽(CTP)治疗乙肝患者50例(慢迁肝23例,慢活肝16例,重症肝炎11例)和HBsAg携带者10例的结果,CTP对消除或减轻临床症状和体征,改善肝功能具有明显的疗效,对慢迁肝,慢活肝和重症肝炎的治疗效果基本相同,各组间无显著性差异。对HBV血清学指标无重要改变,对HBsAg携带者疗效不侍。并对剂景、疗程与疗效的关系进行了讨论。     

Use of the 90Sr applicator for intraoperative radiation therapy in a mouse tumor model

Intraoperative radiation therapy (IORT) research is limited by the lack of small-animal models. We have implanted B16 melanoma into mouse kidneys, which we subsequently operated upon and irradiated with beta rays from a ⁹⁰Sr ophthalmic applicator. The IORT has effectively prolonged survival and produced some cures. The strategy should be applicable to other murine tumors and to other internal implantation sites.     

Importance of seafood as nutrient source in the diet of Belgian adolescents

BACKGROUND: Regular seafood consumption is recommended in dietary guidelines. The aim of this study was to investigate the importance of seafood as a nutrient source in adolescents' diet and the extent to which seafood consumption can increase the intake of omega-3 polyunsaturated fatty acids and vitamin D. METHODS: Consumption data recorded during seven consecutive days for 341 adolescents selected in Ghent (Belgium) were used to estimate the intake of vitamin D, linoleic (LA), alpha-linolenic (LNA), arachidonic (AA), eicosapentaenoic (EPA), docosapentaenoic (DPA) and docosahexaenoic (DHA) acid. RESULTS: The adolescents consumed on average 3.21 microg/day vitamin D, 11.7 g/day LA and 1.4 g/day LNA. The mean intakes of AA, EPA, DPA and DHA were 83.2, 55.9, 18.4 and 111.4 mg/day respectively. The major source of vitamin D was fortified margarine. Fats and oils were the main sources for LA and LNA. The intake of AA was mainly contributed by meat, poultry and eggs. Fish and seafood contributed for 84.1%, 59.3% and 64.4% respectively for EPA, DPA and DHA. CONCLUSION: Flemish adolescents would benefit from increased seafood consumption, as this would lead to a higher intake of EPA and DHA as well as of vitamin D. Moreover, replacement of foods rich in saturated fat (SFA) by seafood products can help to reduce SFA intake.     

The problem of rickets in UK Asians

A large body of work relating to the occurrence of rickets in UK Asians is reviewed. Several theories of the aetiology of this condition are shown to be untenable: it is not exclusively a function of sunlight deprivation or of darker pigmentation; nor is it simply due to phytate-induced losses of calcium from the gut. Asian rickets, however, is associated with a high consumption of cereals, and experiments with rats have suggested a mechanism. In the absence of adequate vitamin D from sunlight, the low-calcium, high cereal intake of the UK Asian population may induce a state of mild secondary hyperparathyroidism which enhances the destruction of vitamin D and leads to a progressive reduction in vitamin D status and, ultimately, to the development of clinical rickets.