黄芪注射液治疗充血性心力衰竭的临床观察

目的 :观察黄芪注射液对充血性心力衰竭 (CHF)的治疗效果。方法 :将 12 7例心衰病人随机分为 2组。对照组 5 5例 ,给予传统治疗心衰药物洋地黄、利尿剂及休息、低盐饮食、吸氧等治疗 ;治疗组 72例 ,在对照组病人用药的基础上加黄芪注射液 40ml ,静脉滴注 ,每日 1次 ,2组均连续用药 15天。结果 :总有效率治疗组 91 7%,对照组 6 7 3%,治疗组效果明显优于对照组 (P <0 0 1) ,且 2组自身对照 ,治疗后好转程度 ,治疗组优于对照组 ,左室射血分数 (LVEF)明显好于对照组。结论 :在常规治疗CHF的同时加用黄芪注射液可显著提高疗效。     

黄芪、女贞、旱莲合剂对小鼠免疫功能的影响

本文报道以黄芪、女贞子、墨旱莲三合剂(AFE)大小剂量(9g/kg、20g/kg)分别给予小鼠灌胃7天,观察其对机体细胞免疫及体液免疫功能的影响。结果表明,两种剂量的AFE均能非常明显地增加小鼠细胞免疫功能(P<0.01),促进其免疫器官正常发育(P<0.05),提高血清IgG水平(>40％)。增加小鼠免疫生化酶一腺苷脱氨酶(ADA)活性,其中大剂量组对ADA的影响具有非常显著性意义(P<0.01)。该合剂还具有恢复环磷酰胺对小鼠免疫器官所致的萎缩作用。提示.该中药合剂可保护、提高小鼠免疫功能。     

黄芪多糖对红斑狼疮小鼠6种抗磷脂抗体的影响

目的 :观察黄芪多糖 (PG2 )对红斑狼疮小鼠抗心磷脂 (anticardiolipin ,aCL)、抗磷脂酰胆碱 (antiphosphatidylcho line ,aPC)、抗磷脂酰丝氨酸 (antiphosphatidylserine,aPS)、抗磷脂酰肌醇 (antiphosphatidylinositol,aPI)、抗磷脂酸 (antiphosphatidicacid ,aPA)和抗磷脂酰乙醇胺 (antiphosphatidylethanolamine ,aPE) 6种自身抗体的影响。方法 :19只雌性NZB×NZWF1小鼠随机分为黄芪I组 (2 5mg kg) 7只 ,黄芪II组 (5 0mg kg) 6只及生理盐水组 (0 2ml d) 6只 ,日一次腹腔注射 ;雌性BXSB及C5 7BL 6小鼠作对照 ,用酶联免疫吸附 (ELISA)法测定各组小鼠的 6种抗磷脂抗体A值进行比较。结果 :NZB×NZWF1小鼠黄芪II组各种抗磷脂抗体A均值比生理盐水组明显降低 (P <0 0 5或P <0 0 1) ,与BXSB ,C5 7BL 6小鼠比无统计学差异 ;黄芪I组与生理盐水组比有所升高 ;黄芪I组及生理盐水组与BXSB、C5 7BL 6比明显升高 (P <0 0 5或P <0 0 1)。结论 :黄芪多糖低剂量有使抗磷脂抗体升高的趋势 ,高剂量可明显抑制抗磷脂抗体的产生     

距骨骨折的手术治疗

目的:探讨距骨骨折的特点、类型、手术方法及影响疗效的相关因素。方法:本组距骨骨折39例,其中男29例,女10例;年龄23~55岁,平均32.5岁。骨折根据Hawkins分型方法:Ⅰ型骨折5例,Ⅱ型骨折25例,Ⅲ型骨折9例。Ⅰ型骨折采用松质骨拉力螺钉内固定。Ⅱ、Ⅲ型骨折行急诊手术,其中Ⅱ型骨折采用前外侧切口暴露,解剖复位,2枚松质骨拉力螺钉交叉内固定;Ⅲ型骨折采用踝前内侧切口暴露,解剖复位,松质骨拉力螺钉内固定,伴有内踝骨折时同时复位内固定,并以自体髂骨块跨骨折线嵌入植骨。术后短腿石膏管形外固定12~24周,X线片示骨折线模糊后拆石膏行不负重下踝关节活动,骨折线消失后恢复伤前活动。结果:除2例失访外,37例获得随访。随访时间1.5~6年,平均3年。根据Hawkins疗效标准评定:优12例(32.4%),良17例(45.9%),可6例(16.2%),差2例(5.4%),优良率为78.4%。结论:距骨骨折通过急诊手术、解剖复位、有效内固定、自体髂骨块嵌入植骨、局部封闭、理疗等综合治疗,可降低病残率。     

Benefits of arthroscopic tuberculoplasty in posterior ankle impingement syndrome

The purpose of this work was to describe the posterior ankle impingement syndrome related to the posterolateral tubercle of the talus bone and to present a retrospective analysis of our results after arthroscopic plasty of the tubercle in 15 ankles with a mean 3-year follow-up. Fifteen cases of posterior ankle impingement (PAI) underwent arthroscopic excision of an impinging bone spur. All the patients (13) were retrospectively evaluated at an average of 36 months after index surgery. There were seven women (bilateral in two of them) and six men. Ten were involved in different kinds of sport and three were professional ballet dancers. Preoperative symptoms included pain localized in the posterior ankle, limitation of motion, weakness and swelling. All patients had failed a course of conservative therapies. Surgery was performed through posterolateral and posteromedial portals as described by van Dijk. After soft tissue debridement, partial resection of the posterolateral process was performed until there was complete plantar flexion without bone impingement. Postoperatively, all patients followed the same rehabilitation protocol. Improvement in their impingement symptoms was recorded in all of them according to AOFAS score. One of them (7%) still had occasional discomfort. The results suggest that arthroscopic bone decompression of the posterolateral tubercle in cases of PAI resistant to non-surgical therapies is an effective treatment.     

老年大鼠脑M_1亚型受体的变化及黄芪的调节作用

目的：观察老年大鼠不同脑区胆碱能Ｍ１亚型受体的变化和黄芪对其的调节作用。方法：采用放射自显影技术显示大鼠脑Ｍ１受体，并用图像分析仪进行灰度分析，以反映Ｍ１受体在不同脑区的相对定量分布。结果：所得脑切片自显影灰度层次清晰，主要分布在大脑皮质、海马、纹状体部位，非特异结合灰度很低，老年大鼠皮质、海马、纹状体的灰度显著低于青年鼠，分别降低１５７８％，８６９％，１２３６％（Ｐ＜００５），老年服黄芪组三个部位的灰度均明显高于老年对照组，分别升高１６６３％，９８１％，１０３２％，（Ｐ＜００５）。结论：黄芪对老年大鼠降低的脑胆碱能Ｍ１亚型受体具有上调作用。     

大黄和黄芪配伍对大鼠减体重的体成分影响研究

目的研究大黄和黄芪配伍在大鼠减体重过程中对体成分的影响。方法大鼠胃饲大黄、黄芪混合水煎剂 ,观察对体重、摄食量、游泳时间、肥体重 (fatbodyweight,FBW )、瘦体重 (leanbodyweight,LBW )、%FBW、脂肪垫、心脏和心脏体重比的影响。结果R1ATr1组 (大黄 :黄芪 1∶1,游泳组 )与CTr组 (游泳对照组 )相比体重、摄食量减少 ,游泳时间延长 (P <0 .0 1) ,FBW和脂肪垫重量减少 (P <0 .0 1)。R1Auntr1组 (大黄 :黄芪 1∶1,不游泳组 )与Cuntr组 (不游泳对照组 )相比 ,体重、摄食量、FBW和脂肪垫重量均减少 (P <0 .0 1)。结论给药大鼠无论运动或不运动 ,体重的减轻主要是FBW减少。提示大黄和黄芪混合水煎剂的作用是促进脂肪动用。     

黄芪多糖对糖尿病大鼠微血管病变的作用及机制的研究

目的：探讨黄芪多糖（APS）对糖尿病大鼠微血管病变的影响以及对糖尿病的治疗作用及机制。方法：成年,健康SD大鼠40只,雌雄不限,分成四组：正常对照组,糖尿病组（腹腔注射四氧嘧啶200mg/kg),APS组（造模成功后用APS治疗）,优降糖组（造模成功后用优降糖治疗）。治疗两周时测一次血糖,四周后,测血糖;颈动脉取血测定血清胰素水平,NO和MDA含量。应用形态定量的方法比较各组的心肌病理变化。结果：腹腔注射alloxan(200mg/kg)后的SD大鼠血糖浓度显升高,血清NO,MDA,胰岛素水平明显改变。APS治疗四周后,血糖浓度明显下降,与优降糖治疗组无明显差异,血清NO较未治疗组明显升高（P＜0．01）,血清MDA较未治疗组明显减少（P＜0．01）,血清胰岛素水平升高（P＜0．05）,光镜下观察,糖尿病大鼠的心肌毛细血管数量减少,基底膜增厚,微血管与心肌纤维的比率显减低,这些改变都可被APS改善.结论：APS有降低血糖和保护血管内皮细胞的作用,这可能与APS减轻氧自由基的损伤,影响NO的产生以及促进胰岛B细胞的损伤的恢复有关。     

薄层扫描法测定还五溶栓胶囊中黄芪甲苷的含量

目的 :测定还五溶栓胶囊中黄芪甲苷的含量。方法 :硅胶G薄层板 ,氯仿 -甲醇 -水 (13∶6∶2 ) 10℃以下放置过夜的下层溶液展开 ,λS=5 30nm ,λR=70 0nm。结果 :线性范围 1.92～ 9.6 0 μg ,97.4 3% ,RSD =3.4 9%。 结论 :该方法可用于还五溶栓胶囊中黄芪甲苷的含量测定     

Application of network pharmacology and molecular docking to elucidate the potential mechanism of Astragalus–Scorpion against prostate cancer

The present study aimed to investigate the molecular mechanism of the Astragalus–Scorpion drug pair in the treatment of prostate cancer (PCa). We employed network pharmacology and molecular docking technology to retrieving the active ingredients and corresponding targets of Astragalus–Scorpion by using TCMSP, BATMAN-TCM, TCMID and Swiss Target Prediction Databases. The targets related to PCa were retrieved through GeneCards. Cytoscape software was used to construct the ‘active ingredient–target disease’ network, and GO and KEGG enrichment analyses were performed on the common targets. Autodock software was used for molecular docking verification. In total, 26 active ingredients, 340 potential targets related to active ingredients and 122 common targets were screened from Astragalus–Scorpion drug pair. The core targets of the protein–protein interaction (PPI) network were JUN, AKT1, IL6, MAPK1 and RELA, whereas the core active ingredients were quercetin, kaempferol, formononetin, 7-o-methylisomucronulatol and calycosin. Nearly 762 GO entries and 154 pathways were obtained by using the pathway enrichment analysis. Molecular docking results revealed that quercetin and kaempferol bind to AKT1 and formononetin binds to RELA, all of which were found to be stable bounds.