全文获取类型
收费全文 | 21532篇 |
免费 | 1809篇 |
国内免费 | 1037篇 |
专业分类
耳鼻咽喉 | 82篇 |
儿科学 | 478篇 |
妇产科学 | 475篇 |
基础医学 | 4682篇 |
口腔科学 | 424篇 |
临床医学 | 1843篇 |
内科学 | 4112篇 |
皮肤病学 | 246篇 |
神经病学 | 1475篇 |
特种医学 | 311篇 |
外国民族医学 | 7篇 |
外科学 | 1139篇 |
综合类 | 3378篇 |
现状与发展 | 5篇 |
预防医学 | 1366篇 |
眼科学 | 270篇 |
药学 | 2063篇 |
2篇 | |
中国医学 | 256篇 |
肿瘤学 | 1764篇 |
出版年
2024年 | 16篇 |
2023年 | 184篇 |
2022年 | 420篇 |
2021年 | 616篇 |
2020年 | 632篇 |
2019年 | 546篇 |
2018年 | 661篇 |
2017年 | 688篇 |
2016年 | 775篇 |
2015年 | 940篇 |
2014年 | 1284篇 |
2013年 | 1603篇 |
2012年 | 1395篇 |
2011年 | 1566篇 |
2010年 | 1336篇 |
2009年 | 1285篇 |
2008年 | 1413篇 |
2007年 | 1402篇 |
2006年 | 1342篇 |
2005年 | 1040篇 |
2004年 | 978篇 |
2003年 | 753篇 |
2002年 | 616篇 |
2001年 | 556篇 |
2000年 | 418篇 |
1999年 | 331篇 |
1998年 | 253篇 |
1997年 | 240篇 |
1996年 | 175篇 |
1995年 | 158篇 |
1994年 | 138篇 |
1993年 | 99篇 |
1992年 | 90篇 |
1991年 | 91篇 |
1990年 | 72篇 |
1989年 | 70篇 |
1988年 | 39篇 |
1987年 | 27篇 |
1986年 | 27篇 |
1985年 | 30篇 |
1984年 | 13篇 |
1983年 | 8篇 |
1982年 | 11篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1979年 | 10篇 |
1978年 | 5篇 |
1976年 | 5篇 |
1971年 | 2篇 |
1906年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
采用Etest药敏试验方法检测 30株临床分离白色念珠菌对 5种抗真菌药物的敏感性 ,并用随机扩增DNA多态性 (RAPD)分型方法对这些菌株进行基因分型。结果发现有两株对氟康唑耐药 ,且它们的RAPD带型有着高度相似性 ,提示RAPD带型可能与耐氟康唑基因有关。 相似文献
112.
Objectives It is likely that genetic factors play a role in the etiology of chronic sinusitis, and airway inflammation is an important pathological feature in chronic sinusitis. We hypothesized that individuals with greater inflammatory responses may be more likely to acquire the disease. Polymorphisms of the tumor necrosis factor (TNF) genes have been described, and certain inflammatory diseases are reportedly associated with certain alleles of TNF genes. The purpose of this study is to examine whether there is an association between some alleles of TNF genes and chronic sinusitis. Study Design Thirty‐eight Japanese patients with intractable chronic sinusitis were selected on the basis of the following criteria: 1) persistent mucous or mucopurulent nasal discharge and/or postnasal dripping for longer than 3 years and 2) opacification in bilateral maxillary sinuses and ethmoid cells on plain radiographic films. Methods Both tumor necrosis factor‐α (TNF‐α) and tumor necrosis factor‐β (TNF‐β) gene polymorphisms were analyzed by polymerase chain reaction (PCR) with restriction fragment length polymorphisms in these patients and 35 healthy control subjects. Results A significantly higher frequency (P < .05) of TNFB*2 allele of TNF‐β gene polymorphism was observed in patients with chronic sinusitis (74%) compared with control subjects (56%). There was no association between alleles of TNF‐α and chronic sinusitis. Conclusion We concluded that TNF‐β gene polymorphism may form a component of the genetic predisposition to chronic sinusitis in Japanese patients. 相似文献
113.
114.
115.
Objective. Angiotensin-converting enzyme (ACE) plays a key role in the metabolism of angiotensin Ⅱ (AT Ⅱ) and inactivation of bradykinins and tachykinins, which are potent bronchialconstrictors and mediators of inflammation asthma, and ACE is heavily expressed in the lungs. An insertion-deletion (D/I) polymorphism of ACE gene has been shown to be associated with levels of ACE. We investigate whether the polymorphism of ACE gene is associated with asthma and bronchial responsiveness.Methods. A case-control study was carried out in 50 asthmatics, 7 families with at least 2 asthmatic individuals, and 50 healthy subjects. The insertion/deletion (I/D) polymorphism of ACE gene was amplified by polymerase chain reaction (PCR). Methacholine brocho-provocation and pulmonary function tests were performed in all asthmatics. Results. There was an higher gene frequency of DD genotype of ACE gene in asthmatic subjects and families individuals compared with healthy subjects (46%, 53% vs 16%, P<0.05; odd ratio 4.98). Anhigher prevalence of DD genotype of ACE was in patients with bronchial hyperresposiveness (BHR) (67%vs 33%, P<0.05; odd ratio 3.8). Accordingly, the mean values of FEV1% and FEV1/FVC were higher in asthmatics carrying non-DD alleles than patients with DD genotype (73.78% vs 56.56%, P<0.05; 79.19% vs 69.29%, P<0.05, respectively).Conclusion. These results suggested that DD allele of ACE genotype was significantly involved in genetic susceptibility to asthma. DD genotype of ACE might be a risk factor for the degree of airway obstruction, it could also be implicated in pathogenesis of bronchial hyperresponsiveness. 相似文献
116.
目的:研究本地区临床分离结核杆菌rpoB基因突变与利福平(RFP)耐药性的关系。方法:对36株RFP敏感株和44株RFP耐药株rpoB基因的328bp的PCR扩增产物进行单链构象多态性(Single-strand comformationpolymorphism,SSCP)分析。结果:36株RFP敏感株的SSCP带谱均与参考株H37Rv相同,44株RFP耐药株中,25株高度RFP耐药株和11株低度RFP耐药株的SSCP带谱与H37Rv带谱有差异;另8株低度RFP耐药株的SSCP带谱与H37Rv带谱相同。结论:SSCP分析可检测出rpoB基因突变,该基因突变与本地区临床分离结核杆菌对RFP耐药有关。 相似文献
117.
Sarah Wang Lina Patel Elise A. Sannar Mellad Khoshnood Natalie K. Boyd Lorena Mendez Noemi A. Spinazzi Eileen A. Quinn Michael S. Rafii Jonathan D. Santoro 《American journal of medical genetics. Part A》2023,191(7):1769-1782
Down syndrome regression disorder (DSRD) is a clinical symptom cluster of acute or subacute neurocognitive regression in otherwise health persons with Down syndrome. The objective of this study was to evaluate if adverse childhood experiences (ACEs) were more prevalent in children with DSRD than those with DS alone. A survey-based, cohort-based study was performed. Caregivers of individuals with DSRD with onset of symptoms between age 10 and 30 years and DS alone were administered the ACEs questionnaire via an online REDCap survey. A total of 159 responses were collected after excluding incomplete surveys and those not meeting criteria for DSRD. Individuals with DSRD were not more likely to experience ACEs (p = 0.18, 95% confidence interval [CI]: 0.43–1.17). In those with ACEs prior to the onset of symptoms, the median time prior was 7 months (interquartile range: 5–10). Individuals with DSRD were more likely to report three or more ACEs (52, 33%) compared to those with DS alone (39, 22%) (p = 0.02, 95% CI: 1.08–2.87). Exposure to ACEs were not predictive of response to particular therapeutic interventions although those with multiple ACEs 3 months prior to the onset of symptoms was associated with lower response rates to benzodiazepines and immunotherapy (p = 0.02, 95% CI: −3.64–−1.13). This study provides preliminary data that individuals with DSRD experience ACEs at a similar rate to individuals with only DS alone, although three or more ACEs, often preceding the onset of symptoms, was more prevalent in individuals with DSRD. 相似文献
118.
U. Zwettler D. Fliser M. Nowicki E. Ritz 《European journal of clinical pharmacology》1994,46(3):185-189
The influence of angiotensin converting enzyme (ACE) inhibition on acute extrarenal and renal potassium elimination in stable chronic renal failure has been examined in 10 male patients median age 44 y; mean CLCR 42 ml·min–1·1.73 m–2. In a double blind, placebo-controlled cross-over study, K+ 0.3 or 0.4 mmol·kg–1 body weight was infused IV on two occasions while the patients also received an infusion either of placebo or 0.5 mg of the ACE inhibitor perindoprilat in random order. Plasma K+ levels and urinary K+ excretion were measured at regular intervals. During the study patients adhered to an isocaloric diet providing a standardised daily intake of potassium and sodium (50 mmol K+ and 40 mmol Na+).The median rise in plasma K+ was not significantly different after placebo ( K 0.66 mmol·1–1) compared with to the infusion of perindoprilat ( K 0.66 mmol·1–1). The median baseline urinary K+ excretion rate was 6.5 mmol·3 h–1 before the placebo infusion and 5.9 mmol·3 h–1 before infusion of perindoprilat. During the potassium load, the urinary excretion rate rose to 16.1 mmol·3 h–1 (after placebo) and 15.1 mmol·3 h–1 after perindoprilat in the first 3 h, and it returned almost to the baseline value within the next 3 h (5.6 mmol·3 h–1 after placebo and 5.7 mmol·3 h–1 after perindoprilat); the differences were not statistically significant.With perindoprilat a decrease in mean arterial blood pressure and ACE activity, an increase in renin plasma activity and a decrease in aldosterone concentrations were observed compared to the placebo infusion. There was no significant differences plasma in adrenaline or insulin levels after either infusion.Thus, ACE inhibition did not interfere either with the extrarenal or the renal disposal of an acute potassium load in patients with chronic renal failure. 相似文献
119.
C. C. Harland G. B. Steventon J. R. Marsden 《European journal of clinical pharmacology》1995,49(1-2):1-6
A pharmacogenetic predisposition to thalidomide-induced neuropathy has been investigated. Differences of drug metabolism were examined in 16 patients with severe orogenital ulceration, who were treated with thalidomide (200 mg/day) for 0.3–5.0 years. Eight had evidence of early peripheral neuropathy according to nerve conduction studies. Rates of C-hydroxylation, N-acetylation, and conjugation reactions with sulphate, glucuronide and glycine, were tested with the probe compounds debrisoquine, sulphadimidine, paracetamol and aspirin, respectively. Urinary drug metabolites were analysed by high pressure liquid chromatography. Results were compared with 16 healthy age- and sex-matched volunteers.Of the patients 6.25% and 13.3% of the controls had a poor Debrisoquine Hydroxylator Ratio (DMR); none of the patients with neuropathy had a poor DMR as compared to 12.5% without neuropathy. Of the patients 40.0% and 35.7% of the controls were slow acetylators; 28.6% with neuropathy were slow acetylators as opposed to 50% without neuropathy. Similarly, there were no significant differences in rates of conjugation between groups. All unaffected patients were active smokers, whereas only two of those with neuropathy smoked. Cumulative dose or duration of therapy were unrelated to risk of neuropathy.In conclusion, changes of nerve conductivity are a frequent and unpredictable adverse effect of thalidomide (200 mg/day), although smoking may have a protective action against their development. Nerve conduction studies are required before and during treatment, irrespective of the prescribed dose. 相似文献
120.
急性白血病患者MGMT基因突变的研究 总被引:1,自引:0,他引:1
目的研究甲基鸟嘌呤-脱氧核糖核酸-甲基转移酶(MGMT)基因突变与急性白血病发病的关系。方法应用聚合酶链反应-单链构象多态性(PCR-SSCP)技术对62例急性白血病中MGMT基因进行了突变分析。结果MGMT基因突变在急性白血病中为14.5%。结论急性白血病中存在MGMT基因突变,其在急性白血病的发生与发展中起到一定作用。 相似文献