Effect of 1,25—Dihydroxyvitamin D3on Gastric Carcinogenesis Induced by N—Methyl—N—nitro—N—nitrosoguanidine in Rats

INTRODUCTlONThehormonallyactiveformofvitaminD3(1,25(OH),D3)hasawellrecognizedroleinregulatingcalciumabsorptionandmineralmetabolism.Inthepastloyears,therehasbeenincreasinginterestintheroleof1,25(OH),D,inregulatingprolifera-tionanddifferentiatlonoftumorcellsincancerssuchasleukemia,breastcarc1noma,coloncarcinomaandmelanoma.(Kogaetal.,1988;Mangelsdorfetal.,1984;Shabahangetal.,1994;Reicheletal.,1989).Theantiproliferatingactivityof1,25(OH)zD3wasalsostudiedinvivo(Eismanetal.,1987;Honmaetal.…     

KMT2C通过c-Myc/CDK4信号通路调控胃癌细胞的生长与增殖

摘要:目的 探究赖氨酸特异性甲基转移酶2C(lysine specific methyltransferase 2C,KMT2C)在胃癌发生发展中的 作用及机制。方法 通过 TCGA 数据库分析 KMT2C在胃癌与癌旁的表达差异。采用 Western blot检测 KMT2C在胃 癌与癌旁临床样本中的表达差异。通过 Kaplan-Meier Plotter数据库分析 KMT2C 对胃癌患者预后的影响。采用细胞 实验(克隆形成、EdU 及 CCK-8检测)及皮下瘤负荷模型检测 KMT2C 在体内外对胃癌细胞增殖能力的影响。结果 KMT2C在胃癌中高表达。胃癌患者中 KMT2C高表达组相对于 KMT2C低表达组预后较差。敲减 KMT2C在体内外 均有抑制胃癌 细 胞 增 殖 的 作 用。基 因 集 富 集 分 析 (GSEA)发 现 KMT2C 影 响 c-Myc信 号 通 路。敲 减 KMT2C 后, H3K4me1蛋白表达水平降低,同时,CDK4的 mRNA 与蛋白表达水平降低。KMT2C与c-Myc核内结合促进了c-Myc 与 CDK4的启动子区域的结合。结论 KMT2C通过影响c-Myc/CDK4信号通路促进胃癌细胞增殖。     

单孔加一孔对比传统腹腔镜全胃切除胃癌根治术的安全性和有效性Meta分析

目的 比较单孔加一孔腹腔镜(SILS+1)和传统腹腔镜(CLS)下行全胃切除胃癌根治术的安全性和有效性。方法 计算机检索PubMed、Web of Science、Embase、中国知网、万方数据库中公开发表的关于SILS+1与CLS辅助下行全胃切除胃癌根治术的安全性和有效性的对照研究,并进行文献质量评价,采用RevMan 5.3对提取的数据进行汇总分析。结果 共6篇回顾性研究纳入本次分析,共计412例,其中SILS+1组204例,CLS组208例。结果显示,与CLS组相比,SILS+1组具有术中出血少[MD=-9.94,95%CI(-18.89,-0.99),P=0.03]、术后并发症发生率低[OR=0.44,95%CI(0.27,0.72),P=0.001]、术后住院时间短[MD=-2.09,95%CI(-2.30,-1.88),P<0.00001]、肛门首次排气时间短[MD=-0.26,95%CI(-0.43,-0.08),P=0.004]的特点,而在手术时间、淋巴结切除数量方面差异无统计学意义(P>0.05)。结论 SILS+1相对于CLS辅助下行全胃切除胃癌根治术...     

羟考酮复合舒芬太尼对腹腔镜胃癌手术患者镇痛效果、苏醒质量及免疫应答的影响

目的:探究羟考酮复合舒芬太尼对腹腔镜胃癌手术患者镇痛效果、苏醒质量及免疫应答的影响。方法:选取93例腹腔镜胃癌手术患者为研究对象,按照麻醉方法不同分为对照组(n=46)和观察组(n=47)。对照组给予舒芬太尼麻醉,观察组给予舒芬太尼复合羟考酮麻醉。比较两组麻醉苏醒质量(Steward评分);术后镇痛效果[视觉模拟评分(VAS)]、补救镇痛情况;麻醉前、术后24 h免疫功能及药物不良反应情况。结果:观察组自主呼吸恢复时间、苏醒时间、拔管时间均低于对照组(P<0.05),Steward评分高于对照组(P<0.05);两组术后1、6、12、24、48 h VAS评分均呈先升后降的趋势(P<0.05),但观察组术后6、12、24 h VAS评分均低于对照组(P<0.05);补救镇痛次数及补救镇痛率均低于对照组(P<0.05);术后24 h,两组CD3⁺、CD4⁺、CD4⁺/CD8⁺比值均降低(P<0.05),但观察组均高于对照组(P<0.05),而两组CD8     

一种新认识的胃癌癌前病变：人胃粘膜球样异型增生的组织病理学特点

Histopathologic features of globoid dysplasia of human gastric epithelium were described by means of observation of serial paraffin sections of 53 cases of globoid dysplasia. It was divided into three grades according to the architecture and cellular atypia. Penetration of outer layer globoid dysplastic cells through the basement membrane of "double layers structure" appeared in typical globoid dysplasia Grade III and infiltration of globoid dysplastic cells into stroma as well as the formation of incipient focus of signet ring cell carcinoma were described. The twinkling scene of infiltration of the globoid dysplastic cells into lamina propria through the basement membrane and the damage of basement membrane by globoid dysplastic cells were shown by Gordon Sweet's stain. Through the analysis of background lesions of the globoid dysplasia, a conclusion can be made that the globoid dysplasia might be an important precancerous lesion of the signet ring cell carcinoma of the stomach.     

The effect of Fufangkushen on gastric cancer cell killing by lines SGC-7901 human γδT cell

Objective To explore the effect of Fufangkushen on gastric cancer cell killing by human γδT cells. Methods Isopentenyl pyrophosphate method was used to amplify human peripheral blood γδT cells in vitro. Fufangkushen at various concentrations was used to induce γδT cells and gastric cancer cell lines SGC-7901 for 24 hours, MTr assays was used to detect inhibitory effect of Fufangkushen on these cell lines, LDH assays was used to measure the cytotoxic activity of γδT cells, and flow cytometry was used to detect apoptosis of γδT cells and SGC-7901 before and after the treatment. Results Ten days after cultivation, proliferation ra-tio of γδT cells increased from 4.21% to 70.35% and CD44 was up to 94.0%. Inhibitory rate of Fufangkush-en on SGC-7901 at various concentrations was significantly higher than that on γδT cells (22.3% vs-22.4%, P<0.05). The negative inhibitory ratio on γδT cells showed a dose-dependent manner with Fufangkushen's concentrations ranging from 1/5 to 1/400. γδT cells cytotoxic activity to SGC-7901 induced by Fufangkushen for 24 h was higher than control group, (83.6% vs 71.2%, P<0.05). Apoptotic rate was significantly lower in γδT cells than in SGC-7901 (4.64% vs49.23%, P<0.05). Conclusion Fufangkushen, within routine concentration ranges, can promote γδT cells' proliferation, inhibit tumor cell growth and enhance γδT cells' cytotoxic activity. This may be beneficial to tumor adoptive immunotherapy and provide evidence for the appli-cation of Fufangkushen in the treatment of tumors.     

别被"抗幽牙膏"忽悠了——科学认识胃中"幽灵"幽门螺旋杆菌

“幽门卫盾,国家专利菌株;预防胃癌,永不口臭;孕妇可用,医用级别……”近期一些不法商家炒作“抗幽牙膏”能够杀灭幽门螺旋杆菌、预防胃癌等噱头,在电商平台售卖所谓“抗幽牙膏”。     

残胃癌及残胃再发癌的外科治疗

统计我院自 1984 - 2 0 0 1年共收治残胃癌及残胃再发癌共 17例 ,其中残胃癌 7例 ,残胃再发癌 10例。全部病例均经过胃镜及病理证实 ,现分析如下 :1　临床资料1 1　见下表1.2　主要症状 :残胃癌及残胃再发癌的早期症状不典型 ,临床表现因发生部位的不同而异。主要表现为中上腹部不适、疼痛、吞咽困难、呕吐、呕血、黑便、消瘦、厌食等。1.3　胃镜所见 :多可见粘膜充血、糜烂、溃疡、肿胀及结节等。癌旁粘膜多伴有萎缩性炎性或萎缩增生性炎性改变 ,特别是吻合口处残胃粘膜常见息肉状增生。1.4　病理学检查 :残胃癌中 ,腺癌 4例 ,低分化腺癌2…     

Hp感染、DNA含量、P~(21)蛋白异常表达与胃癌关系的研究

目的 :明确幽门螺杆菌 (Hp)感染、DNA含量及 P2 1 蛋白在胃炎性病变、不典型增生及胃癌中异常表达的意义 ,探讨三者之间的关系。方法 :Hp、P2 1 蛋白采用免疫组化 (SP)法检测 ;DNA含量采用 Feulgen反应显示 DNA,用北京航天航空大学研制的 CMM- 3彩色病理图像分析系统进行 DNA含量分析。结果 :Hp感染与胃炎性病变及不典型增生密切相关 ,在胃癌中的检出率较前二者低 (34% ) ;不典型增生和胃癌 P2 1 蛋白阳性表达与胃炎性病变 P2 1 蛋白阳性表达有显著性差异 ;中、高增殖病例 P2 1 蛋白阳性表达无显著性差异。结论 :对胃粘膜活检标本同时检测 Hp、P2 1 及 DNA含量 ,对预防胃癌发生有重要的临床意义。另外 ,还可以作为早期诊断胃癌的有效方法。     

以发烧骨痛为首发症状的胃癌1例

1 临床资料 患者男性,63岁,因发烧伴腰背痛于2000年2月10日入院.体温37.3～39℃,夜间升高为著 ,盗汗.腰背部痛呈渐进性加重,活动受限,不能翻身、坐起.既往无结核病史及外伤史. 查体:神清,轻度贫血貌,全身表浅淋巴结无肿大,心肺正常,腹软,无压痛及反跳痛,肝脾肋下未及,T9～10处压痛明显,转体活动受限,四肢运动正常,双下肢无浮肿,NS (-).血常规:Hb 101g/L,RBC 3.1×1012/L,WBC12.1×109/L,P0.80,L0.20,PLT1 22×109/L,ESR65mm/h.末梢血涂片未见异常细胞,ASO<250u,RF(-).肝肾功能及钾、钠、氯、钙、磷均正常.血TB-DNA(-),ALP143.5u/L.ECG(-),胸片(-).胸腰椎X光片示C 9～10椎体骨质破坏.CT示胸腰椎椎体序列正常,生理曲度存在,椎间隙正常,胸腰椎大部分椎体(C9～10为著)及部分附件骨质见虫蚀样破坏,大小不等,多数