载辛伐他汀硫酸钙对去卵巢大鼠胫骨骨缺损修复的影响

目的观察辛伐他汀与硫酸钙混合后对骨质疏松骨缺损修复的促进作用。方法选用健康3月龄雌性SD大鼠48只,切除双侧卵巢,低钙饮食3月,并将胫骨上端造成3 mm骨缺损,随机均分为3组,未植骨组直接缝合皮肤,硫酸钙组在骨缺损处植入硫酸钙,载辛伐他汀硫酸钙组植入载辛伐他汀硫酸钙,植入后4、8周取标本进行组织学观察骨痂形成情况,植入后8周行Micro-CT检测,于12周对标本进行生物力学分析。结果植入4、8周时,载辛伐他汀硫酸钙组骨愈合程度较硫酸钙组和未植骨组有显著性改善,8周时,Micro-CT检测骨缺损区内骨体积分数(BV/TV),组织骨密度(t BMD),骨小梁数量(Tb.N),载辛伐他汀硫酸钙组优于后两组,12周时生物力学检测,载辛伐他汀硫酸钙组失效强度和刚度优于硫酸钙组和未植骨组(P0.05)。结论载辛伐他汀硫酸钙对骨质疏松性骨缺损具有良好的成骨和骨诱导作用,可以较好地促进骨缺损的修复。     

粉刺净胶囊剂质量控制研究

采用薄层色谱法对制剂中的大黄、赤芍进行了定性鉴别 ;用消化—配位滴定法对硫酸锌进行了含量测定 ,方法简便 ,专属性及重现性好 ,可作为该制剂的质量控制指标。     

精索静脉曲张不育与微量元素锌和镉关系的研究

本研究应用原子吸收光谱法 (AAS)测定 18例精索静脉曲张 (VC)不育患者和 14例正常生育的斜疝和或鞘膜积液患者外周静脉血、精索静脉血、精浆中的锌 (Zn)和镉 (Cd)的含量。结果表明 :精索静脉曲张不育患者精静脉血和精浆中Cd含量较对照组明显增高 (P <0 .0 1)、而精浆中的Zn含量较对照组明显减低 (P <0 .0 1)。此外 ,其精索静脉血和精浆中Cd/Zn比值与对照组也有显著性差异 (P <0 .0 1)。提示Cd的增高可能是导致精索静脉曲张不育的原因之一     

云南白药乙醇对长春酰胺血管外渗的防护作用

为了探讨长春酰胺（VDS）静渗的防护方法,将16只新西兰白兔制作成VDS静脉外渗模型,注射部位用白药加50％乙醇湿敷,与50％硫酸镁湿敷作对照,前者效果显著优于后者（均P＜0.01）。证实云南白药乙醇的防护效果后,于197年7月至2000年6月应用于临床15例VDS静脉外汉的肿瘤病人（观察组）,与1996年1月至1997年6月采用50％硫酸镁湿敷的6例VDS静脉外渗者（常规组）对比。结果常规组溃疡发生率显著高于观察组（P＜0.01）。提示云南白药加50％乙醇湿敷对VDS静脉外渗有良好的防护作用,值得推广。     

富锌姬松茸胞内多糖的分离及体内抑制小鼠肝癌的研究

研究了经DEAE-纤维素离子交换柱分离的富锌液态发酵姬松茸胞内多糖组分对小鼠肝癌实体型Heps的体内抑制效果,同时考察了柱层析过后各组分中锌元素的分布情况及给药后小鼠血清锌的变化情况。分离所得组分MP-2在5 mg/(kg.d)剂量下抑瘤率为59.5%(p<0.001),MP-3在10 mg/(kg.d)剂量下抑瘤率为68.1%(p<0.001),两样品在10 mg/(kg.d)剂量下均能使小鼠脾指数显著提高。组分MP-2含锌率3.38‰、MP-3含锌率2.46‰,远高于组分MP-1的含锌率0.12‰;注射样品MP-2、MP-3的实验组小鼠血清锌浓度皆显著高于不给予富锌样品的阴性对照组(p<0.05)。     

保留冠状动脉的猪心螺旋形心室肌带的解剖

Objective Based on the Helical Ventricular Myocardial Band (HVMB) theory proposed by Torrent-Guasp,the ventricular myocardial hand extends from the root of the pulmonary artery to the root of the aorta with two helical coils.This new theory is considered as a revolutionary concept for further understanding the global, three-dimensional and functional architecture of the ven- tricular myocardium. No repot had described techniques for disecting HVMB while keepin～ the integrity of the coronmy artery sys- tern. We explored techniques for dissecting HVMB in swine.Methads 33 fresh swine hearts were randomly divided intoll groups, 3 bearts in each. 160% barium sulfate (type I)suspmmion was injected into the coronary artery system. The coronary arteries were li- gated. The strial tissue was removed following puuing the hearts in boiling water then cooling for several hours. The superficial coro- nary vessels and fat tissue around the atrio-ventricular taxi inter-ventricular sulcus we～'e preserved. Some branches of the left anterior descending artery, distal segment, of posterior descending branch, and middle and distal segment of obtuse marginal branches were mu- tilated appropriately. HVMB dissection was completed with fingers in accordnce with Torrent Guasp' s technique. Results A contin- ued bundle of muscle, originated at the root of pulmonary artery and ended at the root of aorta, was unwrapped along the major dire- tion of the cardiac muscle fiber in all of the 33 hearts with spating of the coronary artery. The swine hearts' ventricular myocandium was cumosed of two loops, with basal loop firm the root of the pulmonart artery to the anterior papillary muscle and apical from the beginning of the anterior papillary muscle to the root tithe aorta. Each loop consisted of two segments: the right segment-coincid- ing with the right ventricular free wall and the left segment-coinciding with the basal d the left ventricular free wall. Posterior papillary muscle, which belongs to the descendant segment, denmrcated the border between the descendent and the ascendant of the HVMB's apical loop. Conclusion Although controversies about the theory of the HVMB remain, we have dissected the HVMB in the swine hearts' ventricular myocardium successfully with sparing of the coronary artery systems. This dissection procedure provides technical information for the studies of associated diseases based on the theory of HVMB.     

Local insulin-zinc injection accelerates skin donor site wound healing

BACKGROUND: Systemically administered insulin has been shown to accelerate wound healing. To minimize the hypoglycemic and hypokalemic effects of insulin, we investigated a new route of insulin administration by local injection into skin wounds. MATERIALS AND METHODS: Partial thickness skin donor site wounds were created on the backs of adult rabbits with a dermatome set at 0.015 inch. The wounds were covered by Aquaphor gauze (Smith and Nephew, Largo, FL), and OpSite membrane (Smith and Nephew, Hull, United Kingdom) and protected by rabbit jackets. Long-acting insulin-zinc suspension was selected for local injection. In study 1, insulin was injected into the wound at different doses, and the concentrations of blood glucose and wound insulin were measured to determine the proper dose and injection frequency. In study 2, wound healing days were compared between two groups (n = 7 each) receiving local injection of either insulin-zinc or zinc alone as control. Based on the results from study 1, a dose of 0.25 units of long-acting insulin-zinc suspension was injected into the wound every other day in the insulin group. RESULTS: After injection, 0.25 units of insulin decreased blood glucose concentration (minimum 60 mg/dL) during the first 3 h, which then returned to the preinjection level (80 mg/dL). One injection maintained wound insulin concentration above 50 muU/mL for more than 24 h. With local injection of 0.25 units insulin-zinc every other day, the wound healing time was 11.2 +/- 2.3 d, which was faster (P = 0.02) than 15.1 +/- 4.1 d in the control group. CONCLUSION: Local injection of long-acting insulin-zinc suspension accelerated skin wound healing without major systemic side effects, demonstrating its potential usefulness in burn treatment.     

特布他林注射液联合3%氯化钠雾化治疗小儿肺炎

目的：探讨特布他林注射液联合3%氯化钠雾化治疗小儿肺炎的临床疗效。方法按照随机数字表法将86例小儿肺炎患儿分为实验组和对照组,实验组患儿给予特布他林注射液联合3%氯化钠雾化吸入治疗,对照组患儿给予小儿肺热咳喘口服液治疗,比较两组临床疗效、用药期间不良反应、临床症状消失时间、体温恢复正常时间及治疗时间。结果实验组患儿治愈率和总有效率均显著高于对照组,差异有统计学意义（P＜0.05）;实验组患儿各临床症状消失时间、体温恢复正常时间及治疗时间均显著低于对照组,差异有统计学意义（P＜0.05）;两组患儿治疗期间均未发现明显不良反应。结论特布他林注射液联合3%氯化钠雾化治疗小儿肺炎治愈率高,能够迅速改善患儿临床症状和体征,缩短治疗时间,临床疗效显著。     

硫酸沙丁胺醇口服液对儿童支气管哮喘的治疗效果

目的：探讨硫酸沙丁胺醇口服液对儿童支气管哮喘的治疗效果。方法2013年4~12月在河南省开封市中心医院门诊接受治疗,经肺功能检查有可逆性气道阻塞,支气管舒张试验阳性的轻、中度哮喘急性发作患儿100例,将其随机分为研究组（n=50）和对照组（n=50）,两组患儿均给予基础治疗,观察组在上述基础治疗上再给予硫酸沙丁胺醇口服液治疗,所有患儿每天早晚监测血压,并记录2周后的哮喘日间与夜间症状评分,晨间用力肺活量（FVC）、第一秒用力呼出量（FEV1）、最大呼气流量占正常预计值之比（PEF%）,并记录治疗效果及不良反应。结果研究组治疗2周后哮喘日间症状评分、哮喘夜间症状评分、临床疗效评分分别为（0.11±0.00）、（0.03±0.01）、（0.04±0.00）分,均低于对照组,差异有统计学意义（P＜0.05）。 FVC、FEV1、PEF分别为（82.0±5.6）%、（84.2±5.2）%、（82.3±3.3）%,均较对照组升高,差异均有统计学意义（均P＜0.05）。两组不良反应事件发生率分别为4.0%、4.0%,组间比较差异无统计学意义（P＞0.05）。结论硫酸沙丁胺醇用于儿童支气管哮喘效果明显,可改善哮喘轻中度急性发作的临床症状及肺功能,缓解支气管平滑肌痉孪,解除喘息,安全性高,可用于治疗儿童支气管哮喘。     

硫酸吲哚酚对大鼠主动脉平滑肌细胞增生影响的机制研究

目的：探讨硫酸吲哚酚（indoxyl sulfate,IS）对大鼠血管平滑肌细胞（vascular smooth muscle cell,VSMC）增生的影响以及这种变化和氧化应激的关系。方法实验均分为8组：正常组、IS 100μM组、IS 300μM组、IS 500μM组、辛伐他汀10μM＋正常组、辛伐他汀10μM＋IS 100μM组、辛伐他汀10μM＋IS 300μM组、辛伐他汀10μM＋IS 500μM组。采用WST-1法检测VSMC增殖情况;硫代巴比妥酸法检测培养基中丙二醛（malondialdehyde,MDA）含量;ELISA方法测定晚期氧化蛋白产物（advanced oxidation protein products,AOPP）。结果与正常组比较,IS 300μM组和 IS 500μM 组上清液的 WST-1（OD 值）[（1.55±0.27）比（1.18±0.25）与（1.73±0.30）比（1.18±0.25）,P〈0.01]含量、MDA含量[（2.60±0.47）μg/ml比（1.59±0.21）μg/ml与（2.82±0.54）μg/ml比（1.59±0.21）μg/ml,P〈0.01]和 AOPP 含量[（67.94±8.58）μmol/L 比（54.97±8.46）μmol/L与（72.09±9.49）μmol/L比（54.97±8.46）μmol/L,P〈0.01]均明显增高。与同浓度 IS 组相比,辛伐他汀10μM＋IS 300μM 组和辛伐他汀10μM＋IS 500μM 组上清液 WST-1（OD 值）[（1.22±0.24）比（1.55±0.27）与（1.32±0.30）比（1.73±0.30）,P〈0.05]、MDA[（1.64±0.38）μg/mL 比（2.60±0.47）μg/ml 与（1.70±0.40）μg/ml 比（2.82±0.54）μg/ml,P〈0.01]含量和 AOPP 含量[（55.56±7.41）μmol/L比（67.94±8.58）μmol/L 与（55.54±6.80）μmol/L 比（72.09±9.49）μmol/L,P〈0.05]均明显降低。大鼠 VSMC 上清液的 WST-1含量与 MDA 含量呈正相关（r=0.621,P〈0.01）,大鼠VSMC上清液的WST-1含量与AOPP含量呈正相关（r=0.581,P〈0.01）。结论 IS可浓度依赖性地促进大鼠 VSMC增生,其作用可能与 IS增加 VSMC的氧化应激有关;辛伐他汀可抑制此作用。