Comparison of antigenic and allergenic components of Holoptelea integrifolia pollen collected from different source materials

Antigenic extracts prepared from pollen samples collected at weekly intervals during the same season did not exhibit significant variation in protein concentration. Stored pollen samples from different years, however, showed highly significant variations in protein concentration. The protein content of samples from different ecozones of India also varied (CV = +/- 32%). The IEF and SDS-PAGE patterns were almost identical in samples from the same season, but were variable in the samples stored from different years and different parts of India. IgE binding proteins from different samples also varied depending on the overall protein profiles. Almost all the patients, however, showed IgE binding to four proteins at 50, 60, 66 and 70 kD, indicating the important allergenic components of Holoptelea integrifolia.     

高原油菜蜂花粉对缺氧动物心、肝、肺组织超微结构的影响

用高原油莱蜂花粉喂养动物后进行耐缺氧试验,取动物心、肝、肺组织后用电镜观察,结果表明高原油莱蜂花粉在机体缺氧时,对组织起微结构的损伤有明显的保护作用,从而使机体在缺氧环境下提高组织细胞对氧的利用,加速机体适应于低氧环境。     

NiTi,317L金属浸提液对犬食管成纤维细胞增殖功能的影响

目的：研究ＮｉＴｉ、３１７Ｌ两种合金的浸提液对培养的犬食管壁成纤维细胞增殖功能的影响。方法；采用ＮｉＴｉ、３１７Ｌ金属浸提液与原代培养食管壁成纤维细胞共培养，ＭＴＴ法检测不同培养时间Ｆｂ增殖功能的变化。结果 ＮｉＴｉ，３１７Ｌ金属浸提液对Ｆｂ增殖功能具有轻度抑制作用，ＮｉＴｉ金属浸提液较３１７Ｌ浸提液作用稍强。结论：ＮｉＴｉ，３１７Ｌ金属浸提液对食管壁Ｆｂ的增殖功能无促进作用。     

参附注射液用于老年患者全麻诱导期对血流动力学的影响

目的 观察参附注射液用于老年患者全身麻醉诱导期间对血流动力学的影响.方法 选择60-75岁手术患者50例随机分为两组,参附组25例于麻醉诱导前静注参附注射液0.5ml/kg,对照组25例于麻醉诱导前静注乳酸林格氏液0.5ml/kg,5min后麻醉诱导;两组均静注咪唑安定0.1mg/kg、罗库溴铵8mg/kg、芬太尼3μg/kg、异丙酚1.5mg/kg麻醉诱导.于麻醉前静息状态下、诱导后即刻、插管后即刻、插管后1、2、3、5、10min分别测定收缩压(SBP)、舒张压(DBP)、心率(HR)并记录.结果 参附组患者在麻醉诱导及插管时血流动力学较为稳定,且优于对照组.结论 参附注射液可有效维持老年患者全身麻醉诱导期间循环的稳定.     

水蛭提取液对凝血酶引起的体外星形胶质细胞毒性损害的保护作用研究

目的 探讨凝血酶对培养的大鼠脑星形胶质细胞毒性损害及水蛭提取液对其的保护作用. 方法 建立体外实验即大鼠脑星形胶质细胞培养实验模型,相差显微镜观察细胞生长状况.MTT法筛选水蛭提取液的有效浓度.星形胶质细胞分为水蛭提取液治疗组与凝血酶对照组,测定培养上清液酸脱氢酶(LDH)活性(细胞死亡的标志).免疫组化法观察它们的HSP70和TGFβ-1的表达. 结果 (1)一定浓度范同(1～100 U/mL)的凝血酶对星形胶质细胞有毒性作用,且随凝血酶剂量的增大,它对星形胶质细胞的毒性作用相应增加(F=118.65,P=0.000).(2)一定浓度范围内(0.25～4 mg/μL)的水蛭提取液能明显减轻10 U/mL凝血酶对星形胶质细胞的毒性作用(F=156.08,P=0.000),且随水蛭提取液浓度的增大,保护作用增强,还可促进星形胶质细胞HSP70和TGFβ-1的表达. 结论 水蛭提取液能促进星形胶质细胞增生,增强它们的HSP70和TGFβ-1表达,从而明显抑制凝血酶对星形胶质细胞的毒性作用.     

Immunotropic activity of plant extract echinosol

The formulation of medicinal herbal tea Echinosol was developed. Immunotropic properties of aqueous-alcohol extract Echinosol were studied. This extract (300 mg/kg) had a stimulatory effect on the following parameters: formation of T cell precursors and production of T cell effectors; migration of lymphocytes, monocytes and neutrophils into the blood; digestive activity of neutrophils; migration of monocytes and lymphocytes into the peritoneal exudate; expression of C_3b receptors on macrophages; phagocytic capacity of cells due to the existence of C_3b receptors; number of antibody-producing cells in the spleen; and synthesis of immunoglobulins. Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 146, No. 8, pp. 188–191, August, 2008     

Primary versus secondary immunoglobulin E sensitization to soy and wheat in the Multi-Centre Allergy Study cohort

Background IgE sensitization to soy and wheat is classified as ‘primary’ when generated by food ingestion and ‘secondary’ when it as a consequence of primary sensitization to cross‐reacting pollen antigens via inhalation. The age‐specific relevance of these categories of sensitization throughout childhood is unknown. Objective To monitor the natural course of IgE sensitization against common food allergens in childhood in relation to sensitization against cross‐reactive airborne allergens. Methods The German Multi‐Centre Allergy Study with follow‐up from birth to age 13 recruited initially 1314 children. IgE antibody levels against cow's milk, hen's egg, soy, wheat, mites, cat and dog dander, birch and grass pollens were tested. Longitudinal data were analysed from the 273 children with sera obtained at age 2, 5, 7 and 10 years of age. Results The point prevalence of sensitization (>1.0 kU/L) to milk and egg allergens progressively decreased from about 4% at 2 years to <1% at 10 years. By contrast, the prevalence of IgE to wheat and soy progressively increased with age, from 2% to 7% (soy) and from 2% to 9% (wheat). At 10 years of age, IgE to grass pollen was detected in 97% and 98% of the children reacting against soy and wheat, respectively; IgE to birch pollen was observed in 86% and 82% of the children reacting against soy and wheat, respectively. Early IgE sensitization to soy or wheat preceded that to grass or birch pollen in only 4% and 8% of participants sensitized to soy and wheat, respectively. Conclusion IgE sensitization to soy and wheat is relatively uncommon and mostly primary in early infancy, more frequent and mostly secondary to pollen sensitization at school age. Clinical Implications Awareness should be raised to avoid unnecessary diet restrictions due to the high frequency of clinically irrelevant, secondary sensitization to soy and wheat in schoolchildren with pollinosis.     

In vitro studies in reproductive immunology. - 2. Demonstration of the inhibitory effect of male genital tract constituents on PHA-stimulated mitogenesis and E-rosette formation of human lymphocytes

The effect of male genital tract components (human spermatozoa, intact and chromatographed seminal plasma fractions) on in vitro cell-mediated immune reactions was examined. Their addition to PHA-containing lymphocyte cultures resulted in a marked degree of inhibition of DNA synthesis, the response varying with the fractions employed. The interference by such components with a terminal cell event during blast transformation was suggested by the failure of stimulated cultures to incorporate thymidine irrespective of the time during the incubation period at which the constituents were added. Seminal plasma and sperm extracts were also shown to inhibit T-cell associated E-rosette formation. The inhibitory properties of genital fractions remained unaltered after repetitive freeze-thaw cycling when tested in the transformation and E-rosette assays. Heating to 100 degrees C modified the response with some of the fractions.     

脑泰方提取物抗肿瘤坏死因子诱导人脐静脉内皮细胞凋亡的研究

目的 观察重组人肿瘤坏死因子α(rhTNF-α)诱导人脐静脉内皮细胞(HUEC)凋亡及中药脑泰方提取物(NTE)的作用。方法 将融合生长的HUEC随机分为空白对照组(正常培养，不加rhTNF-α)、NTE对照组(加中剂量NTE，不加rhTNF-α)、rhTNF-α组(仅加100ngmL rhTNF-α)和NTE高、中、低剂量组(加100ng/mL rhTNF-α和6mg/mL、2mg/mL、0．67mg/mL NIE)，观察rhTNF-α作用24h后内皮细胞吖啶橙/溴乙啶双重荧光染色和凋亡细胞计数。结果 荧光显微镜下可见，空白对照组和NTE对照组HUEC为正常结构的活细胞、染色质主要着亮绿色，凋亡细胞率分别为14．0％和14．5％；rhTNF-α组与NTE低剂量组细胞大多呈均匀橙色，染色质多被染为橘红色，以固缩状或圆珠状为主，凋亡细胞率分别为75．0％和63．5％；NTE高、中剂量组细胞多呈亮绿色，凋亡细胞率分别为43．5％和53．0％。结论 一定浓度的肿瘤坏死因子引起的内皮细胞损伤主要表现为细胞凋亡，脑泰方提取物可明显抑制这种凋亡的过度发生。