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991.
Chad Ellermeier Emily C. Higuchi Naina Phadnis Laerke Holm Jennifer L. Geelhood Genevieve Thon Gerald R. Smith 《Proceedings of the National Academy of Sciences of the United States of America》2010,107(19):8701-8705
During meiosis, the formation of viable haploid gametes from diploid precursors requires that each homologous chromosome pair be properly segregated to produce an exact haploid set of chromosomes. Genetic recombination, which provides a physical connection between homologous chromosomes, is essential in most species for proper homologue segregation. Nevertheless, recombination is repressed specifically in and around the centromeres of chromosomes, apparently because rare centromeric (or pericentromeric) recombination events, when they do occur, can disrupt proper segregation and lead to genetic disabilities, including birth defects. The basis by which centromeric meiotic recombination is repressed has been largely unknown. We report here that, in fission yeast, RNAi functions and Clr4-Rik1 (histone H3 lysine 9 methyltransferase) are required for repression of centromeric recombination. Surprisingly, one mutant derepressed for recombination in the heterochromatic mating-type region during meiosis and several mutants derepressed for centromeric gene expression during mitotic growth are not derepressed for centromeric recombination during meiosis. These results reveal a complex relation between types of repression by heterochromatin. Our results also reveal a previously undemonstrated role for RNAi and heterochromatin in the repression of meiotic centromeric recombination and, potentially, in the prevention of birth defects by maintenance of proper chromosome segregation during meiosis. 相似文献
992.
Mechanical properties of thermoplastic polyurethane elastomers based on either polyether or polycarbonate (PC)-glycols, 4,4’-dipheylmethane diisocyanate (1,1’-methylenebis(4-isocyanatobenzene)), 1,4-butanediol, were controlled by restriction of crystallization of polymer glycols. For the polyether glycol based-polyurethane elastomers (PUEs), poly(oxytetramethylene) glycol (PTMG), and PTMG incorporating dimethyl groups (PTG-X) and methyl side groups (PTG-L) were employed as a polymer glycol. For the PC-glycol, the randomly copolymerized PC-glycols with hexamethylene (C6) and tetramethylene (C4) units between carbonate groups with various composition ratios (C4/C6 = 0/100, 50/50, 70/30 and 90/10) were employed. The degree of microphase separation and mechanical properties of both the PUEs were investigated using differential scanning calorimetry, dynamic viscoelastic property measurements and tensile testing. Mechanical properties could be controlled by changing the molar ratio of two different monomer components. 相似文献
993.
994.
总结了制药分离工程课程被评为郑州大学精品课程后,所做的教学实践与教学改革工作,通过总结课程建设的经验,反映制药分离工程精品课程建设的特色。 相似文献
995.
目的分析临床金黄色葡萄球菌(SA)分布特征及耐药性,并建立可用于检测葡萄糖球菌A蛋白(SPA)的新方法。方法分析132株SA菌株的分布特征与耐药性。构建金属螯合免疫磁性微球,对其表面形态、大小、表面基团吸收峰进行测定,用该磁性微球分离SPA,并用于SA检测。结果 132株SA分离自痰液74株(占56.06%)、分泌物43株(占32.58%)、血液10株(占7.57%)、胆汁3株(占2.23%)、器械导管2株(占1.52%)。检出甲氧西林敏感SA(MSSA)61株(占46.21%),对青霉素G、左氧氟沙星、庆大霉素、克林霉素、红霉素的耐药率分别为93.12%、13.23%、23.12%、56.87%、54.23%;甲氧西林耐药SA(MRSA)71株(占53.79%),对青霉素G、复方磺胺甲噁唑、左氧氟沙星、庆大霉素、克林霉素的耐药率分别为92.12%、21.21%、61.21%、29.21%、76.23%。磁性微球分散性良好,对SA样品的检测灵敏度可达100CFU,在一定条件下可维持高活性3周。结论 SA菌株耐药性较强;免疫磁性微球技术可用于SA的快速检测,值得推广应用。 相似文献
996.
膜分离与沉淀剂结合提取腌肠衣盐水中肝素纳的工艺研究 总被引:1,自引:0,他引:1
目的探讨膜分离与沉淀剂结合提取腌肠衣盐水中肝素纳的工艺改进。方法采用盐解-离子交换-膜分离及蛋白沉淀除杂法从中提取肝素纳。结果从废弃的腌肠衣盐水中提取肝素钠取得了成功,与传统工艺(盐解-离子交换法)比较,洗脱液体积可浓缩至原体积的15%左右,乙醇用量可减少85%左右,而效价分别从传统工艺的96.82U/mg增加到157.58U/mg,总收率比传统工艺提高了12.5%。结论工艺方法可行、可靠,适宜于中、小型规模的生产。 相似文献
997.
Early catastrophic failure of a modular polyethylene component is a potential problem after revision total hip arthroplasty. We describe an unusual case of polyethylene failure that occurred within 18 months of implantation in which no obvious technical error or mechanical failure was identified. The acetabular polyethylene insert was prepared with gas plasma sterilization, and the shelf life was 4 months. Radiographic evaluation used generalized Hough transforms to assess the cup articulation. We identified cup penetration of 2.8 mm before revision and catastrophic destruction of the polyethylene liner at the time of revision. Possible factors implicated for failure included a thin polyethylene liner, increased hip separation, femoral head mismatch, and the high activity level of a younger patient. We believe that this case report highlights the need for future investigations of these subtle factors. 相似文献
998.
Mayman DJ González Della Valle A Lambert E Anderson J Wright T Nestor B Sculco TP Salvati EA 《The Journal of arthroplasty》2007,22(4):624-629
Five patients presented with fiber metal mesh shedding of a Harris Galante II acetabular cup detected between 11 and 15 years after implantation. All patients presented with hip pain and 4 demonstrated gross acetabular loosening and fiber metal separation on preoperative radiographs. The remaining patient underwent revision surgery because of a liner dislodgment and had a radiographically well fixed shell. Loosening and fiber metal separation were detected intraoperatively. Scanning electron microscopy of the retrieved shells demonstrated isolated diffusion bonding marks in the areas where the mesh separated from the substrate and no evidence of corrosion. Progressive osteolysis in the iliac bone was evident in 4 of our cases. Progressive iliac osteolysis may lead to loss of bone support in well-fixed cups and excessive stresses transferred to the interface between the fiber metal mesh and the titanium substrate leading to the separation of the 2 layers. Fiber metal separation may contribute to long-term loosening in the Harris Galante acetabular component. 相似文献
999.
药房托管作为一种医药分开的过渡模式,顺应专业化管理的社会分工趋势,有利于减轻患者的疾病经济负担。但是,药房托管模式在运行中存在“内部人控制”,受托企业利润低、人事管理困难,在三级医院推广困难等问题。本文对此进行了分析,并提出了对策。 相似文献
1000.
胰岛细胞移植是治愈糖尿病的可能方法 ,但胰岛细胞数量有限,不能满足临床需要. 肝卵圆细胞可以在体外诱导分化为胰岛细胞,因此可以作为胰岛细胞潜在的替代资源.文章就肝卵圆细胞及其定向诱导分化为胰岛细胞的相关研究作一综述. 相似文献