EORTC QLQ—C30生命质量测定量表在维吾尔族恶性肿瘤患者中的应用评价

目的：将欧洲EORTC生命质量核心量表QLQ—c30（V3）中文版翻译成维吾尔文,并对其进行信度、效度,反应度、适应度检验及可行性分析,使量表可以应用于维吾尔族肿瘤人群。方法：通过对EORTCQLQ—C30（V3）中文版的翻译、回译及部分化调适,产生维文量表,用其对497例维吾尔族恶性肿瘤患者进行生命质量测定。所得资料用统计学方法进行相关分析及配对检验,最终对量表的信度、效度、反应度,可行性等进行考评。结果：①内部一致性信度：各领域的克朗巴赫系数（Cronbach’so）均在0．681以上;重测信度：除了腹泻,恶心呕吐等单项测试项目以外,P值均大于0．05;②结构效度：30个条目,共15个维度,提取了6个公共因子,累积方差贡献率为54．62％;因子分析结果表明,调查分析结果和理论构想基本吻合。③反应度：除了呼吸困难,便秘等项目外,其余领域及单项测量项目P值均小于0．05,表示量表能较好反映治疗前后生命质量的差异。④量表的可行性：回收率为96．42％,完成率为95．44％,完成量表的时间15min以内。结论：经翻译、回译、文化调适后产生的EORTCQLQ—C30（V3）维吾尔文版具有较好的信度、效度、反应度,可以用于维吾尔族恶性肿瘤患者的生活质量评价。     

脂必泰对高脂血症患者CD40/sCD40L的调节作用

目的 探讨脂必泰对高脂血症患者CD40/sCD40L水平的影响.方法 66例原发性高脂血症患者应用脂必泰治疗8周,分别测定治疗前（0周）、治疗4周和8周后CD40和sCD40L的浓度进行对比,同时入选44例健康者作为正常对照组.结果 高脂血症组与正常对照组相比CD40/sCD40L治疗前表达明显上调,而应用脂必泰治疗4周后sCD40L即有显著下降（P〈0.05）;CD40治疗后亦有下降,但未达到统计学意义.结论 高脂血症刺激细胞CD40/sCD40L表达显著增加,脂必泰具有抑制CD40/sCD40L信号转导通路的作用,这可能是其抗炎、抗动脉硬化的重要机制.     

LncRNA HIF1A-AS2 accelerates malignant phenotypes of renal carcinoma by modulating miR-30a-5p/SOX4 axis as a ceRNA

Objective: Several reports have proposed that lncRNAs, as potential biomarkers, participate in the progression and growth of malignant tumors. HIF1A-AS2 is a novel lncRNA and potential biomarker, involved in the genesis and development of carcinomas. However, the molecular mechanism of HIF1A-AS2 in renal carcinoma is unclear.Methods:The relative expression levels of HIF1A-AS2 and miR-30a-5p were detected using RT-qPCR in renal carcinoma tissues and cell lines. Using loss-of-function and overexpression, the biological effects of HIF1A-AS2 and miR-30a-5p in kidney carcinoma progression were characterized. Dual luciferase reporter gene analysis and Western blot were used to detect the potential mechanism of HIF1A-AS2 in renal carcinomas.Results:HIF1A-AS2 was upregulated in kidney carcinoma tissues when compared with para-carcinoma tissues (P < 0.05). In addition, tumor size, tumor node mestastasis stage and differentiation were identified as being closely associated with HIF1A-AS2 expression (P < 0.05). Knockdown or overexpression of HIF1A-AS2 either restrained or promoted the malignant phenotype and WNT/β-catenin signaling in renal carcinoma cells (P < 0.05). MiR-30a-5p was downregulated in renal cancers and partially reversed HIF1A-AS2 functions in malignant renal tumor cells. HIF1A-AS2 acted as a microRNA sponge that actively regulated the relative expression of SOX4 in sponging miR-30a-5p and subsequently increased the malignant phenotypes of renal carcinomas. HIF1A-AS2 showed a carcinogenic effect and miR-30a-5p acted as an antagonist of the anti-oncogene effects in the pathogenesis of renal carcinomas.Conclusions:The HIF1A-AS2-miR-30a-5p-SOX4 axis was associated with the malignant progression and development of renal carcinoma. The relative expression of HIF1A-AS2 was negatively correlated with the expression of miR-30a-5p, and was closely correlated with SOX4 mRNA levels in renal cancers.     

间变性大细胞淋巴瘤一例报道

间变性大细胞淋巴瘤(anaplastic large cell lymphoma,ALCL)是WHO最新淋巴瘤分类中一个较少的类型。上海交通大学医学院第三人民医院2006年4月收治1例患者,现报告如下。1临床资料患者,男性,16岁。右眼突出伴复视2周,发热、咳嗽2 d。门诊眼眶核磁共振(MRI):右侧眼眶内球后上方异     

CK18-M30和内毒素在大鼠非酒精性脂肪肝病中的促进作用

目的探讨细胞角蛋白18-M30片段(CK18-M30)、内毒素在大鼠非酒精性脂肪肝病(NAFLD)转化为非酒精性脂肪性肝炎(NASH)中的促进作用。方法 26只大鼠随机分为3组,对照组(NC组):普通饮食;NAFLD组:高脂饮食喂养8周;NASH组:高脂饮食喂养12周。生化仪测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST);光度法测定血清内毒素(ET);ELISA检测血清IL-18、CK18-M30,RT-PCR分析粪便肠道菌群;行苏木精伊红(HE)染色观察肝脏及末端回肠组织病理。结果 NAFLD及NASH组相比NC组,小肠黏膜结构由轻到重受到破坏。NAFLD组及NASH组大鼠血清ET、CK18-M30、肝脂肪变性程度及NAFLD活动度积分(NAS)、大肠杆菌属计数显著高于NC组,而双歧杆菌属、乳酸杆菌属计数显著低于NC组,差异有统计学意义(P<0.05);NASH组大鼠血清ET、CK18-M30、肝脂肪变性程度及NAS显著高于NAFLD组,差异有统计学意义(P<0.05);NASH组大鼠血清ALT、AST、IL-18显著高于NC组及NAFLD组,差异有统计学意义(P&l...     

Exploratory COVID-19 death risk score based on basic laboratory tests and physiological clinical measurements

BACKGROUND: In the event of a sudden shortage of medical resources, a rapid, simple, and accurate prediction model is essential for the 30-day mortality rate of patients with COVID-19.METHODS: This retrospective study compared the characteristics of the survivals and nonsurvivals of 278 patients with COVID-19. Logistic regression analysis was performed to obtain the “COVID-19 death risk score”(CDRS) model. Using the area under the receiver operating characteristic(AUROC) curve and Hosmer-Lemesho...     

Sudomotor dysfunction is frequent and correlates with disability in Friedreich ataxia

Objectives

To evaluate autonomic symptoms and function in Friedreich’s Ataxia (FRDA).

抗p30血清的制备与鉴定

作者用分离和纯化获得的p30免疫家兔,制备特异性抗p30血清。鉴定结果表明,该抗血清仅与p30发生反应,与猪、狗、牛、羊、兔、大鼠、小鼠、鸡等8种动物精液;猪、狗、牛、鸡等4种动物血清;人阴道分泌液、初乳、血清、唾液、尿液等5种人体液;人阴道粘膜、子宫内膜、蜕膜、绒毛、卵巢、乳腺、睾丸、附睾、脑、扁桃、甲状腺、胸腺、心、肺、脾、肝、胰、胃、小肠、结肠、肾上腺、肾与膀胱等23种人体组织均无交叉反应,具有高度的种属特异性和男性生殖器官特异性。     

尿液可溶性CD163对IgA肾病临床预后的预测价值研究

目的探讨尿液可溶性巨噬细胞生物标志物CD163（sCD163）对IgA肾病（IgAN）临床预后的预测价值。方法选取原发性IgAN患者78例,均行肾脏穿刺病理检查,检测尿液sCD163水平。根据病理检查肾小球中新月体的百分比将患者分为C0组（无新月体形成）、C1组（新月体百分比＜25%）、C2组（新月体百分比≥25%）,比较3组患者尿液sCD163水平;分析尿液sCD163水平与肾脏穿刺时的平均动脉压（MAP）、尿蛋白肌酐比、血肌酐、估算的肾小球滤过率（eGFR）及肾小球新月体百分比的相关性;以尿液sCD163水平＜1.03为尿液sCD163低水平组,尿液sCD163水平≥1.03为尿液sCD163高水平组,比较两组患者临床预后情况。结果C2组患者尿液sCD163水平高于C0、C1组患者（5.67±7.49vs0.69±0.76、0.57±0.67,均P＜0.05）。尿液sCD163水平与尿蛋白肌酐比、血肌酐、肾小球新月体百分比呈正相关（r=0.787、0.271、0.217,均P＜0.05）,与eGFR呈负相关（r=-0.299,P＜0.05）。尿液sCD163高水平组患者的肾脏生存率低于低水平组患者（84.35%vs98.18%,P＜0.05）。结论尿液sCD163可作为预测IgAN临床预后的一项无创性生物标志物。IgAN患者高尿液sCD163提示预后不良。     

聚乙二醇化重组人粒细胞集落刺激因子大鼠和Beagle犬的免疫原性

目的：在进行聚乙二醇化重组人粒细胞集落刺激因子（PEG30-rhG-CSF）大鼠和Beagle犬重复给药毒性试验时,观察给药后动物血清中抗PEG30-rhG-CSF抗体的产生和抗体的中和活性,为非临床安全性评价的确切性以及临床给药周期提供依据。方法：大鼠分为赋形剂对照组、PEG30-rhG-CSF 0.75、3.0、12 mg / kg组,sc给药,隔日1次,连续2周;Beagle犬分为赋形剂对照组、PEG30-rhG-CSF 0.25、1.0和4.0 mg / kg,sc,每周1次,连续4周。采用ELISA法检测动物血清中抗PEG30-rhG-CSF的抗体,采用NFS-60细胞／MTT比色法检测抗体的中和活性。结果：大鼠在给药期和恢复期各剂量组动物血清中均未检测到抗PEG30-rhG-CSF的结合抗体。Beagle犬在给药的第1周和第2周,未检测到结合抗体,但在第4周时,低、中和高3个剂量组各有5只（5/6）的动物血清中检测到结合抗体,而抗体滴度与剂量无明显相关性。恢复4周后,仅高剂量组1只（1/2）动物出现抗体,且抗体滴度呈下降趋势。另外,在给药期和恢复期,血清中所产生的抗体均无中和PEG30-rhG-CSF的活性。结论：大鼠重复给予PEG30-rhG-CSF的毒性试验中,所有动物血清中均未检测到结合抗体和中和抗体;Beagle犬用药组的绝大部分动物血清中出现抗PEG30-rhG-CSF的抗体,但所产生抗体无中和活性。