CD30在变应性鼻炎患者鼻黏膜组织中的表达

目的 :探讨CD30在变应性鼻炎患者鼻黏膜组织中的表达 ,分析其在变应性鼻炎发病中的作用。方法 :应用免疫组织化学SP法和苏木精 伊红染色对 31例变应性鼻炎患者的下鼻甲黏膜 (变应性鼻炎组 )和 2 6例肥厚性鼻炎患者的下鼻甲黏膜 (对照组 )标本中CD30的表达进行检测 ,并进行统计学分析。结果 :变应性鼻炎组CD30细胞数显著高于对照组 ,其差异有统计学意义 (P <0 .0 5 )。结论 :在变应性鼻炎的鼻黏膜组织中存在着Th2细胞主导的黏膜反应 ,通过研究CD30的表达水平可了解变应性鼻炎中Th2反应的状态。     

间变性大细胞淋巴瘤1例报告

介绍诊治的1例间变性大细胞淋巴瘤的临床特征、形态学、免疫表型及遗传学特征,并复习相关文献.     

Description of Methicillin-Susceptible Staphylococcus aureus Clonal Complex 30 Related to the Pandemic Phage Type 80/81 Isolated from Patients in Three Tertiary Hospitals in Jos,North Central Nigeria

ObjectiveThe prevalence of phage 80/81 Staphylococcus aureus strains, the pandemic strains that were dominant in the 1950s, had declined in the 1960s and 1970s. However, these strains have reemerged in some countries in recent years. This study investigated the antibacterial resistance, virulence, and the genetic backgrounds of CC30-MSSA isolates obtained from patients in three tertiary hospitals.Materials and MethodsTwenty-two CC30-MSSA isolates cultured from different clinical samples were investigated using antibiotic sensitivity testing, spa typing, multilocus sequence typing, and DNA microarray analysis.ResultsAll 22 isolates were susceptible to vancomycin (MIC ≤2 μg/mL), teicoplanin (MIC ≤2 μg/mL), and cefoxitin but were resistant to penicillin G (n = 22; 100.0%), tetracycline (n = 12; 54.5%), ciprofloxacin (n = 15; 68.2%), cadmium acetate (n = 22; 100%), mercuric chloride (n = 13; 59.1%), and ethidium bromide (n = 3; 13.6%). The isolates belonged to sequence type, ST30, and five spa types: t012 (n = 12; 54.5%), t019 (n = 5; 22.7%), t017 (n = 2; 9.1%), t037 (n = 2; 9.1%), and t318 (n = 1; 4.5%). All 22 isolates were positive for agrIII, cap8, clfA, clfB, icaA, icaC, icaD, cna, and staphylococcal enterotoxin gene clusters (seg, sei, sem, sen, seo, seu). Eight isolates carried lukS-PV and lukF-PV that code for Panton-Valentine leukocidin.ConclusionThe current CC30-MSSA isolates share phenotypic and genotypic characteristics with the pandemic phage 80/81 isolates that were common in the 1950s and 1960s. Continued surveillance is recommended to keep abreast of the changing epidemiology of S. aureus causing healthcare and community-associated infections.     

CK18-M30和内毒素在大鼠非酒精性脂肪肝病中的促进作用

目的探讨细胞角蛋白18-M30片段(CK18-M30)、内毒素在大鼠非酒精性脂肪肝病(NAFLD)转化为非酒精性脂肪性肝炎(NASH)中的促进作用。方法 26只大鼠随机分为3组,对照组(NC组):普通饮食;NAFLD组:高脂饮食喂养8周;NASH组:高脂饮食喂养12周。生化仪测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST);光度法测定血清内毒素(ET);ELISA检测血清IL-18、CK18-M30,RT-PCR分析粪便肠道菌群;行苏木精伊红(HE)染色观察肝脏及末端回肠组织病理。结果 NAFLD及NASH组相比NC组,小肠黏膜结构由轻到重受到破坏。NAFLD组及NASH组大鼠血清ET、CK18-M30、肝脂肪变性程度及NAFLD活动度积分(NAS)、大肠杆菌属计数显著高于NC组,而双歧杆菌属、乳酸杆菌属计数显著低于NC组,差异有统计学意义(P<0.05);NASH组大鼠血清ET、CK18-M30、肝脂肪变性程度及NAS显著高于NAFLD组,差异有统计学意义(P<0.05);NASH组大鼠血清ALT、AST、IL-18显著高于NC组及NAFLD组,差异有统计学意义(P&l...     

Stratification of Gallstones in the Gallbladder

A clinical approach to the diagnosis of congenital cardiac malformations is described which the authors have found effective in exposing the important signs and their significance. Each available diagnostic method is evaluated separately before all data are correlated.

Clinical diagnosis based on history, physical examination, roentgenography and electrocardiography was 94 per cent accurate in a series of cases seen in office consultation and 90 per cent accurate in a series of hospital cases reviewed. The significance of the various signs and symptoms that are routinely searched for in the physical examination is discussed.

The salient points in the clinical diagnosis of the common entities are presented.     

Duchenne肌营养不良患者的脊柱融合

目的：观察肌营养不良患者脊柱固定融合术的疗效。方法：对１９８２～１９９５年接受脊柱融合的１２例肌营养不良患者的病历和Ｘ线片进行回顾性研究。随访时间不少于１年。资料包括患者术前肺功能、手术步骤、随访时Ｘ线片Ｃｏｂｂ角和骨盆倾斜程度及患者功能状态。结果：手术时患者年龄为１３５岁,术前肺功能为５６％,脊柱侧凸平均４８°,骨盆倾斜平均１５°,所有患者均接受后路融合双鲁氏棒固定术和髂骨植骨。最后随访时没有发现不融合病例,脊柱侧凸平均３１°,骨盆倾斜平均８°,所有患者均能够坐位而无不适。结论：后路融合双鲁氏棒固定术有助于维持肌营养不良患者的脊柱序列,建议对该类患者在脊柱侧凸发展至３０°前行脊柱融合术。     

过氧化氢干预下Rab30在PC12细胞中的表达及促凋亡通路

目的探讨经过氧化氢(H_2O_2)损伤干预下Rab30在PC12细胞中的表达及可能的促凋亡通路。方法将体外常规培养的PC12细胞分为正常组和H_2O_2组。采用MTT检测细胞存活率;Annexin V-FITC/PI双染法检测细胞凋亡率;Western-blot检测Rab30、JNK3、GM130、Caspase-3的蛋白表达情况。结果 H_2O_2损伤干预可导致PC12细胞的存活率显著降低(P0.05),早期凋亡率和总凋亡率均显著增高(均P0.05);JNK3、Caspase-3蛋白表达水平在PC12细胞中显著增加(P0.05),而Rab30、GM130蛋白表达显著下降(P0.05)。结论 H_2O_2在PC12细胞中的促凋亡通路可能是H_2O_2激活JNK3的表达而靶向下调Rab30,致使高尔基体结构破碎,并激活Caspase-3的活性,从而诱导PC12细胞的凋亡。