多层螺旋CT三期增强扫描对肝腺瘤的定量分析

目的分析肝腺瘤的多排螺旋CT影像特征。方法对11例行三期增强扫描的肝腺瘤患者进行分析。结果11例中共发现19个病灶,其CT值在平扫、动脉期、延迟期存在统计学差异,门脉期无统计学差异。动脉期直径≤5cm病灶CT值高于5cm病灶,而其余各期无统计学差异。结论多层螺旋CT三期增强扫描结合定量分析有助于肝腺瘤的诊断及鉴别诊断。     

Dorsal root ganglion neurons promote proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Preliminary animal experiments have confirmed that sensory nerve fibers promote osteoblast differentiation, but motor nerve fibers have no promotion effect. Whether sensory neurons promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells remains unclear. No results at the cellular level have been reported. In this study, dorsal root ganglion neurons(sensory neurons) from Sprague-Dawley fetal rats were co-cultured with bone marrow mesenchymal stem cells transfected with green fluorescent protein 3 weeks after osteogenic differentiation in vitro, while osteoblasts derived from bone marrow mesenchymal stem cells served as the control group. The rat dorsal root ganglion neurons promoted the proliferation of bone marrow mesenchymal stem cell-derived osteoblasts at 3 and 5 days of co-culture, as observed by fluorescence microscopy. The levels of m RNAs for osteogenic differentiation-related factors(including alkaline phosphatase, osteocalcin, osteopontin and bone morphogenetic protein 2) in the co-culture group were higher than those in the control group, as detected by real-time quantitative PCR. Our findings indicate that dorsal root ganglion neurons promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells, which provides a theoretical basis for in vitro experiments aimed at constructing tissue-engineered bone.