复方中药含药血清对人A375黑素瘤细胞酪氨酸酶活性的影响

目的探讨复方中药含药血清对人A375黑素瘤细胞酪氨酸酶（TYR）活性的影响。方法体外培养人A375黑素瘤细胞,采用多巴速率氧化法研究复方中药含药血清对TYR活性的影响。结果低浓度（5%～10%）的Ⅰ方、Ⅱ方、Ⅲ方对酪氨酸酶活性无明显激活作用（P〉0.05）;高浓度（20%～30%）的Ⅰ方、Ⅲ方、Ⅵ方对TYR活性均有明显激活作用（P〈0.05）,以Ⅵ方作用最显著,且与其它各组相比,差异均有统计学意义（P〈0.01）。结论对TYR具有激活作用的中药组成复方并不一定对TYR有激活作用,具有祛风活血和补肾三重作用的Ⅵ方在促进TYR活性上优于祛风除湿Ⅰ方、养血活血Ⅱ方和滋补肝肾Ⅲ方。     

Interventions promoting physical activity among obese populations: a meta‐analysis considering global effect,long‐term maintenance,physical activity indicators and dose characteristics

As the benefits that regular physical activity (PA) have on obesity are well known, many interventions promote active lifestyle adoption among obese populations. This meta‐analysis aims to determine (i) the global effect that interventions promoting PA among obese populations have on their PA behaviour; (ii) variations in the effect of interventions depending on the PA indicator used; (iii) the programme's dose characteristics and (iv) maintenance of the intervention effects after the intervention has ended. A comprehensive search through databases and review articles was completed. Forty‐six studies met the inclusion criteria. Calculations of effect size (Cohen's d) and a moderator analysis were conducted. The meta‐analysis showed that interventions globally have an impact on the PA behaviour of obese populations (d = 0.44; 95% CI = 0.31, 0.57). The moderator analysis revealed that interventions of less than 6 months reported significantly larger effects than longer interventions. Moreover, the interventions had a stronger impact on the number of steps and the PA indexes (i.e. composite scores reflecting PA practice) than on other PA indicators. Finally, the analysis revealed that interventions succeed in maintaining PA behaviour after the intervention is over. However, relatively few studies addressed this issue (n = 9). Despite global positive effects, further research is needed to determine the optimal dose for interventions and to evaluate the maintenance of intervention effects.     

Is physical activity a part of who I am? A review and meta-analysis of identity,schema and physical activity

Two parallel literatures on the physical activity (PA) identity and schema constructs have the potential to supplement traditional social cognitive approaches used for PA promotion. The purpose of this paper was to review schema/identity research and appraise its relationship with PA via meta-analysis followed by thematic analyses of its correlates, as well as its proposed mechanisms on PA. Eligible studies were from English, peer-reviewed published articles that examined identity and/or schema in the context of PA. Searches were completed in June 2015 in five databases. Sixty-two independent data-sets (32 available for meta-analysis), primarily of modest quality, were identified. Results of the random effects meta-analysis showed that the point-estimate between identity/schema and behaviour was r?=?.44 (CI?=?.39?.48), and invariant to selected study characteristics. Thematic review showed that identity/schema was associated with commitment, ability, affective judgments, identified/integrated regulation and social comparison and predicted intention, self-regulatory efficacy, and self-regulation strategy use. It had reliable evidence as a moderator of the intention–behavior relationship, was associated with increases in the speed of processing of relevant information and created negative affect under hypothetical identity–behavior discrepant situations. While this initial research is promising, more rigorous research designs, including interventions to increase identity/schema, are warranted.     

Using Fitness Trackers in Clinical Research: What Nurse Practitioners Need to Know

Consumer-based wearable fitness trackers present a new array of opportunities and challenges to nurse practitioners engaged in health promotion research. Key advantages include the ability to provide continuous, objective, remote monitoring of physical activity and the potential to improve the efficacy of physical activity interventions. This article provides an overview of fitness trackers, including their functions and accuracy, and addresses the following key issues to consider before using trackers in research: 1) when to use a fitness tracker, 2) choosing a brand and model, 3) encouraging good compliance, and 4) extracting and using the data.     

GC-MS analysis,determination of total phenolics,flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves

ObjectiveTo perform phytochemical screening, estimate total phenolics, flavonoids and to evaluate antioxidant potential of Moringa peregrina (M. peregrina) leaves.MethodsThe dried powdered leaves of M. peregrina (150 g) were extracted exhaustively by Soxhlet with ethanol and then fractionated into hexane, chloroform, ethy alacetate and methanol. All the prepared extracts were also analyzed by gas chromatography-mass spectrometry to identify and characterize the chemical compounds present in the crude extracts. Folin- Ciocalteu reagent and aluminium chloride colorimetric methods were used to estimate total phenolic and flavonoid content of extracts. Hydrogen peroxide and 1,1 diphenyl -2-picrylhydrazyl were used to determine in vitro antioxidant activity.ResultsPhytochemical analysis of ethanol extract showed presence of major classes of phytochemicals. Gas chromatography-mass spectrometry results revealed presence of 19 phytoconstituents in hexane extract, 6 in ethyl acetate and 7 compounds in methanolic extract. Methanol extract was found to contain the highest phenolic content and flavonoids. In vitro antioxidant activities of all crude extracts were significant and comparable with the standard ascorbic acid.ConclusionsResults of this study show that the leaves of M. peregrina are the rich source of phenolic compounds that can play an important role in preventing the progression of many diseases.     

Effect of progressive muscular relaxation exercises versus transcutaneous electrical nerve stimulation on tension headache: A comparative study

Tension-type headache (TTH) is most frequent among all types of headaches. According to the International Headache Classification Subcommittee of the International Headache Society (2004), TTH occurs in 30–78% of the population. Progressive muscular relaxation exercises have been shown to reduce TTH, and home-based relaxation programmes can result in significant improvement in headaches. Transcutaneous electrical nerve stimulation (TENS) is a method of electrical stimulation that primarily aims to provide a degree of symptomatic pain relief by exciting sensory nerves and stimulating either the pain gate mechanisms or the opioid systems. The objective of this study was to compare between the effects of progressive muscular relaxation exercises and TENS on pain intensity and stress in people with TTH. Thirty patients with TTH were allocated to either Group A or Group B. Group A practised progressive muscular relaxation exercise, whereas Group B received TENS. In the latter group, TENS electrodes were placed bilaterally either on the head at the site of pain or on the occiput. The treatment was carried out for 15 minutes a day, for 7 days. Patients were assessed for pain intensity (Visual Analogue Scale) and level of stress (Lakaev Academic Stress Response Scale) before and after the intervention period. The results showed that progressive muscular relaxation exercises were effective in reducing pain as well as stress (p < 0.001). TENS, by contrast, reduced stress significantly (p < 0.001), but not pain (p = 0.233). Between-group analysis revealed that there was no statistically significant difference in reduction of pain between the two groups (p = 0.595), but the amount of stress reduction in Group A (p = 0.002) was significantly more than that in Group B. In conclusion, progressive muscular relaxation exercises were more effective in reducing stress level than TENS in patients with TTH. The effect on pain reduction was similar between the two treatment methods.