蜂蜜干膏的质量研究

目的 建立蜂蜜干膏的质量标准.方法 采用文献调研、性状、检查和含量测定等方法进行研究.结果 观察了样品的性状特征,建立了样品的检查和含量测定方法.结论 所建立的标准可用于蜂蜜干膏的质量控制.     

Simultaneous determination of jujuboside A, B and betulinic acid in semen Ziziphi spinosae by high performance liquid chromatography-evaporative light scattering detection

A reverse phase high performance liquid chromatographic (HPLC) method with evaporative light scattering detection (ELSD) was developed for simultaneous determination of jujuboside A, B and betulinic acid in semen Ziziphi spinosae. The analysis was performed by gradient elution, using an aqueous mobile phase (containing 0.1% acetic acid) modified by acetonitrile. The evaporator tube temperature of ELSD was set at 45 °C, and with the nebulizing gas flow-rate of 1.8 l/min. The method was validated for accuracy, reproducibility, precision and limits of detection and quantification. Quantification of the three active compounds in semen Ziziphi spinosae from different locations was performed by this method, which provides a new tool for quality assessment of semen Ziziphi spinosae.     

甘草次酸C3、C30衍生物的合成及其体外抗肿瘤活性研究

目的设计并合成甘草次酸C3、C30衍生物,并对其体外抗肿瘤活性进行研究。方法以甘草次酸为先导化合物,对其C3位羟基、C30位羧基进行结构修饰,并采用SRB法对目标化合物进行体外抗肿瘤活性研究。结果设计合成了12个新型甘草次酸衍生物,并利用MS、1H-NMR及元素分析确证了结构;体外实验中,目标化合物对MCF-7和A549肿瘤细胞的抑制活性均明显强于甘草次酸,其中化合物GA-I1、GA-I2和GA-II1对MCF-7和A549两种细胞表现出很好的抑制活性,明显高于对照药吉非替尼。结论甘草次酸衍生物具有良好的抗肿瘤活性,值得进一步研究。     

NMR Studies of Retinoid-Protein Interactions: The Conformation of [13C]-β-Ionones Bound to β-Lactoglobulin B

Purpose. Vitamin A (retinol) and its metabolites comprise the natural retinoids. While the biological action of these molecules are thought to be primarily mediated by ca. 55 kDa nuclear retinoic acid receptors, a number of structurally similar 15-20 kDa proteins are involved in the transport, and possibly metabolism, of these compounds. The milk protein -lactoglobulin B (-LG) is an 18 kDa protein which binds retinol and may be involved in oral delivery of retinol to neonates. -LG also binds drugs and other natural products and is of potential interest as a protective delivery vehicle. Methods. To examine the conformation of the model retinoid -ionone both in solution and when bound to -LG, NMR and computational methods have been employed. Results. Taken together, NMR studies of -ionone in solution measuring scalar and dipolar coupling, as well as CHARMm calculations, suggest -ionone prefers a slightly twisted 6-s-cis conformation. Isotope-edited NMR studies of ^l3C-labeled -ionones bound to -LG, primarily employing the HMQC-NOE experiment, suggest -ionone also binds to -LG in its 6-s-cis conformation. Conclusions. The methods employed here allow estimates of protein-bound ligand conformation. However, additional sites of ligand labeling will be necessary to aid in binding site localization.     

透明质酸在肿瘤治疗药物新型给药系统中的应用

透明质酸（hyaluronic acid, HA）因其良好的理化性质和肿瘤靶向性,已作为药物载体或者靶向因子应用于肿瘤治疗的新型给药系统中,并成为肿瘤治疗研究的热点。本文主要对HA在新型给药系统的应用进行介绍。     

HPLC法测定银灵康胶囊中绿原酸和落新妇苷的含量

目的建立同时测定银灵康胶囊中绿原酸和落新妇苷含量的方法。方法采用HPLC法。色谱柱为Kromasil C18柱(150 mm×4.6 mm,3.5μm),流动相为乙腈(A)-体积分数0.05%磷酸水溶液(B),梯度洗脱,柱温为30℃,检测波长为291 nm。结果绿原酸质量浓度在9.6～96 mg·L-1内与峰面积呈良好的线性关系(r=0.999 9),平均回收率为98.4%(RSD=1.6%,n=9);落新妇苷质量浓度在0.550～5.50 mg·L-1内与峰面积呈良好的线性关系(r=0.999 5),平均回收率为100.8%(RSD=1.8%,n=9)。结论建立的方法简便﹑准确﹑重现性好,可用于银灵康胶囊的质量控制。     

用于卡介菌多糖核酸制剂效力实验的小鼠模型的建立

目的  建立用于卡介菌多糖核酸制剂效力实验的小鼠模型。方法  选用猪血清、牛血清白蛋白、卵白蛋白作为致敏原,腹腔注射BALB/c小鼠以建立Ⅰ型变态反应动物模型,并通过在卡介菌多糖核酸制剂效力实验中应用建立的小鼠模型来验证其可行性。结果  猪血清、牛血清白蛋白和卵白蛋白均可作为致敏原致敏BALB/c小鼠,但牛血清白蛋白和卵白蛋白的致敏效果优于猪血清。卡介菌多糖核酸制剂效力实验显示,牛血清白蛋白致敏小鼠建立的动物模型可更好地反映卡介菌多糖核酸制剂的效力。结论  牛血清白蛋白致敏小鼠建立的动物模型可用于卡介菌多糖核酸制剂效力实验。