深低温对吸入麻醉药的MAC,心脏麻醉指数和心肌稳定性的影响

目的:研究深低温对吸入麻醉药MAC、心脏麻醉指数和心肌稳定性的影响。方法:新西兰白兔40只,随机分为氟烷、安氟醚、异氟醚和七氟醚组。采用夹尾试验法测定常温下(38℃±0.5℃)的最低肺泡有效浓度(MAC)。行体表降温后测定深低温下(23℃±0.5 ℃)的MAC。维持深低温增加吸入性麻醉药的浓度,同时用50Hz、25V电压胸外电击心脏。记录出现室颤或室性心律失常时的肺泡呼气末吸入麻醉药浓度。结果:从38℃到23℃兔体温每降低1℃,MAC下降值为:氟烷5.1％、安氟醚3.6％、异氟醚4.4％、七氟醚4.3％;氟烷、安氟醚、异氟醚和七氟醚心脏麻醉指数分别为4.4、3.18、6.25和4.6,异氟醚明显高于其它麻醉药;麻醉药浓度8MAC以内安氟醚和氟烷发生室颤的机率(100％)明显高于七氟醚和异氟醚(40％)。结论:异氟醚是深低温麻醉的最佳选择用药,而安氟醚则不宜用于低温麻醉。     

青黛颗粒抗炎、镇痛作用的实验研究

目的 :通过对青黛颗粒抗炎、镇痛作用的实验研究 ,探讨该药治疗溃疡性结肠炎的作用机理。方法 :采用小鼠醋酸扭体法、大鼠棉球肉芽肿法和角叉菜胶所致大鼠足肿胀方法。结果 :青黛颗粒给药高、中、低剂量组和阳性对照药组均明显降低小鼠扭体次数 (P<0 .0 5～P<0 .0 1)。对大鼠棉球肉芽肿和大鼠足肿胀有显著的抑制作用 (P <0 .0 1～P <0 .0 0 1)。结论 :青黛颗粒分别对大鼠具有抗炎、对小鼠具有镇痛作用。且镇痛作用呈量效关系     

25-Hydroxyvitamin D3 metabolism in a human leukemia cell line

Summary 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) is a potent inducer of monocytic differentiation of the human promyelocytic leukemia cell line, HL-60. We have noted that 25-hydroxyvitamin D₃ (25(OH)D₃) in high doses is also capable of promoting monocytic differentiation of this cell line. To test the possibility that the latter activity is due to conversion of 25OHD₃ to 1,25(OH)₂D₃ by HL-60, we exposed HL-60 cells to 25OHD₃ and analyzed the products by HPLC and radioreceptor assay. When chromatographed in the traditional solvent system (isopropanol-hexane), a new peak appears which migrates with authentic 1,25(OH)₂D₃. However, in a solvent system containing dichloromethane, 90% of the peak migrates with another metabolite, 19-Nor-10-Keto-25OHD₃ (19-Nor-25OHD₃). Production of this metabolite is enhanced by living cells and is synthesized by both virgin HL-60 and those which have undergone differentiation. We next determined if authentic 19-Nor-25OHD₃ also promotes differentiation of this cell. As assessed by appearance of the monocyte-specific surface antigen (63D3) and macrophage-specific esterase activity, we find that this metabolite does, in fact, induce monocytic differentiation of HL-60 with a potency of approximately 1/200 that of 1,25(OH)₂D₃ and similar to that of 25OHD₃. In agreement with the effect upon cell maturation, 19-Nor-25OHD₃ displaces³H-1,25(OH)₂D₃ from its HL-60 receptor with an efficiency comparable to 25OHD₃. Hence, HL-60 cells convert 25OHD₃ to 19-Nor-25OHD₃, and 19-Nor-25OHD₃ induces monocytic differentiation of HL-60 with comparable efficiency to its precursor, 25OHD₃.     

蝌蚪提取液诱导人早幼粒白血病细胞分化的实验研究

目的 :探讨蝌蝌提取液 (T871)对白血病细胞的诱导分化作用。方法 :利用细胞生长曲线、形态学观察、细胞免疫化学及原位mRNA杂交和完整细胞原位斑点印迹技术 ,观察了T871诱导HL 60细胞分化的作用和分化过程中癌基因c myc、c myb表达的变化。结果 :T871能抑制HL 60细胞的增殖 ,形态学表现为类似单核 /巨噬细胞 ,同时T871还能使HL 60细胞的NBT还原能力、ANAE活性显著增强 ,同时伴有癌基因c myc、c mybmRNA表达的下调。结论 :T871能诱导HL 60细胞向单核 /巨噬细胞方向分化 ,c myc、c myb癌基因可能参与了此过程     

培养大鼠星形胶质细胞牵张损伤后超微结构的变化

目的研究体外培养星形细胞牵张损伤后超微结构的变化。方法取新生1~2 d大鼠的皮层细胞原代培养,经纯化后传代培养于乳胶膜培养皿中。采用计算机控制的牵张损伤装置,分别以50、150、250 kPa压力牵张损伤培养于乳胶膜上的大鼠皮层星形胶质细胞。用3%戊二醛固定后,行扫描电镜和透射电镜观察。结果当用50 kPa驱动压力进行牵张损伤时,细胞结构即有明显破坏,表现为细胞间隙增宽,部分胞体和突起被撕裂;以50 kPa牵张损伤后1 h,透射电镜下可见线粒体肿胀、嵴减少;损伤后6 h可见线粒体致密、细胞器减少等。当牵张应力增大,星形细胞损伤程度加重,细胞器明显减少,线粒体空泡化,微丝、微管明显减少,直至水样胞质或致密胞体。结论较小的应力即可致星形细胞紧密连接的破坏和超微结构的改变,可能与脑外伤后产生广泛的脑水肿有关。     

冠心病中医辨证与载脂蛋白关系的初步研究

对９６例各种类型冠心病患者进行中医辨证分型，检测其血清载脂蛋白ＡⅠ及Ｂ１００（ａｐｏＡⅠ及ａｐｏＢ１００），并设立对照组。结果显示各证型冠心病患者的ａｐｏＡⅠ，ａｐｏＢ１００及ａｐｏＢ１／ａｐｏＡⅠ均有不同程度的改变。在标实证中以痰浊型改变最为明显，其ａｐｏＡⅠ显著降低，ａｐｏＢ１００及ａｐｏＢ１００／ａｐｏＡⅠ显著升高。在本虚证型中以肾虚型改变最为明显、其ａｐｏＡⅠ显著降低。ａｐｏＢ１００／ａｐｏＡⅠ显著升高。这种各证型间的差异无疑对冠心病的中医辨证客观化有一定意义，值得进一步验证和探讨。     

Cellular localization of immunoreactive epidermal growth factor during Wolffian duct differentiation of the fetal mouse

Previous studies from this laboratory indicated a role for epidermal growth factor (EGF) in androgen-dependent male reproductive tract differentiation of the fetal mouse. Expression of an EGF-like protein during Wolffian duct differentiation was indicated from the determinations by radioimmunoassay (RIA) and radioreceptor assay. To further characterize the protein and to assess its role in male sexual differentiation, expression of the protein has been analyzed by Western blot assay and its tissue-specific cellular expression has been determined by immunocytochemical assay in the present study. Western blot analysis of the 18-day fetal male reproductive tract detected an immunoreactive band of the predicted 6-kDa size. Immunocytochemical analysis also detected EGF-specific immunostaining in the Wolffian duct derivatives. At day 18 of gestation, the staining was localized predominantly in the epithelial nuclei of the Wolffian duct derivatives whereas at days 14 and 16 of gestation, the staining was equally distributed in the mesenchymal and epithelial sites of the Wolffian duct derivative. The intensity of the staining increased with progression of differentiation during the 14th–18th days of gestation. Prenatal exposure to the antiandrogen flutamide significantly reduced the immunostaining of the duct. Thus, a role for EGF in Wolffian duct differentiation is indicated.     

同型半胱氨酸对胚胎海马神经元细胞的影响

目的 观察同型半胱氨酸(homocysteine，HCY)对海马神经元细胞分化和增殖的作用机理。方法 采用大鼠胚胎海马神经元细胞进行体外培养。观察不同浓度的HCY(0．01、0．1、1．0、10．0、100．0)mmol／L对细胞分化和增殖的影响。结果 随着剂量的增加．同型半胱氨酸对胚胎海马神经元细胞分化和增殖具有抑制作用，并用流式细胞术分析证明对脂质过氧化(LPO)的影响而使得神经元蛋白质含量增高。结论 同型半胱氨酸可抑制胚胎海马神经元细胞分化和增殖的影响，HCY可能在导致神经管畸形的过程起着重要作用。     

Mucinous adenocarcinoma with neuroendocrine differentiation of the mandibular ramus: report of a case

A rare case of mucinous adenocarcinoma with neuroendocrine differentiation of the mandibular ramus is presented. The patient, an 80-year-old man, was referred to our hospital with chief complaint of swelling and pain in the left buccal mucosa. CT and MRI examination showed an osteolytic tumor mass occupying the upper region of the left mandibular ramus. Macroscopically, the excised tumor was a relatively well-defined, solid mass with diffuse bone resorption, measuring 3 cm x 3.2 cm x 3 cm. Microscopical examination showed that the tumor forming glandular structures with abundant mucous production and high cellular atypia. Immunohistochemical studies demonstrated the positive reactivities for pan-keratin, cytokeratin 7, vimentin,alpha-amylase, alpha-smooth muscle actin, neuron-specific enolase, glial fibrillary acid protein, calcitonin, and somatostatin in tumor cells. These findings suggested that the tumor was originated from heterotopic or misplaced salivary gland in the mandible.