高龄初产妊高征孕妇过氧化反应的研究

目的：探讨高龄初产妊高征孕妇体内过氧化反应。方法：测定正常初产妇、妊高征孕妇妊娠晚期母血过氧化脂质（LPO）、超氧化物歧化酶（DOD）的含量。结果：1．高龄初产妇母血LPO较非高龄初产妇明显升高，SOD明显下降，P＜0．05。孕妇年龄与母血LPO呈明显正相关，与SOD呈明显负相关，P均＜0．05。2．妊高征孕妇LPO较正常孕妇显著增高，SOD显著下降，P＜0．05，并随病情加重LPO水平升高、SOD水平下降更为显著，P＜0．05。3．高龄初产妊高征孕妇母血LPO含量较非高龄初产妊高征孕妇明显增高，P＜0．05，而SOD则无显著变化，P＞0．05。4.妊高征孕妇胎儿宫内生长发育迟缓（IUGR）发生率较正常孕妇显著升高，P＜0．025，各组脐血清LPO、SOD含量无显著差异，P＜0．05。结论：高龄初产妊高征孕妇体内过氧化作用明显增强，LPO水平的升高可能为高龄初产妇妊高征发生率及IUGR发生率增加的原因之一。     

Modified Scleral Flap Incision to Reduce Corneal Astigmatism after Intraocular Lens Implantation

To investigate a simple method during extracapsular cataract extraction with posterior chamber intraocular lens implantation in order to reduce surgically induced corneal astigmatism.Methods: A modified scleral flap incision was used in the extracapsular cataract extraction with intraocular lens implantation and the postoperative changes in conreal astigmatism was observed.Results: The peak value of postoperative corneal astigmatism was 3. 60 D, and the corneal astigmatism regression was 2. 11 D, surgically induced astigmatism was less significant in modified scleral flap incision group than that in conventional limbal incison group (P<0. 05).Conclusions: The modified scleral flap incision is an ideal incision for cataract extraction with intraocular lens implantation when phacoemulsifier is not available. Eye Science 1995; 11-. 136-139.     

红景天苷对3T3-L1脂肪细胞糖代谢及细胞分化的影响

目的:观察红景天苷对脂肪细胞糖代谢和细胞分化的影响,分析其改善糖代谢的可能机制.方法:检测红景天苷干预的脂肪细胞对 3H-葡萄糖的摄取,对红景天苷干预分化的细胞进行油红O染色后通过比色法分析脂肪细胞分化程度.逆转录多聚酶联反应(RT-PCR)检测脂肪细胞分化相关基因过氧化物体增殖剂活化受体-γ(PPAR-γ)、CAAT/增强子结合蛋白-α(C/EBP-α) mRNA的表达. 结果:红景天苷组葡萄糖摄取率为110.4%,明显高于正常对照组 (P<0.01);红景天苷明显抑制细胞分化及PPAR-γ、C/EBP-α mRNA表达,与对照组比较,P<0.01.结论:红景天苷促进3H-葡萄糖摄取,可抑制脂肪细胞分化,下调分化相关基因的表达.     

In vitro growth of epithelial cells from mucosa derived from different regions of the gastrointestinal tract

Attempts have been made to culture the mucosa from various parts of the gastrointestinal tract. In this study, using an explant culture method, epithelial cells have been successfully cultured from all major regions of the gastrointestinal tract. The success rate, as judged by outgrowth of epithelial cells for at least 4 weeks, varied with the tissue studied with 19/50 colonic biopsies, 5/11 small intestinal biopsies, 9/12 stomach biopsies and 42/47 gallbladder biopsies yielding outgrowth of epithelial cells. Differentiation of the epithelial cells along the mucus cell pathway could be demonstrated on the monolayer cultures using Periodic acid Schiff or Alcian blue staining. Because the cultures were very heterogeneous and many morphological cell types were present in most cultures, differentiation along the other known differentiation pathways of the gastrointestinal mucosa, such as development of absorptive cells and endocrine cells, could not be excluded.
The problem of bacterial contamination, which has hindered previous studies on tissue from these sites, was overcome by decontaminating the biopsy by soaking in dilute sodium hypochlorite (0.04%).     

星形胶质细胞源性因子对神经干细胞分化的实验研究

目的探讨星形胶质细胞源性因子对神经干细胞分化的影响。方法分离和培养新生大鼠脑组织的神经干细胞;采用差速贴壁法和振荡法分离纯化星形胶质细胞,用免疫细胞化学染色法,胶质纤维酸性蛋白(GFAP)标记星形胶质细胞,进行细胞的纯度鉴定;将星形胶质细胞和神经干细胞在互不接触的情况下进行共培养,免疫荧光法观察神经干细胞分化后神经元特异性烯醇化酶(NSE)、GFAP和酪氨酸羟化酶(TH)的表达。结果纯化的星形胶质细胞GFAP抗体标记阳性,细胞纯度达98%;星形胶质细胞与神经干细胞共培养时,神经干细胞贴壁分化加快,NSE阳性细胞及TH阳性细胞明显多于对照组(P<0·05)。结论星形胶质细胞源性因子可快速诱导神经干细胞向神经元细胞、包括多巴胺神经元细胞分化,提示星形胶质细胞支持神经元发生。     

脑肿瘤干细胞体外分化过程中的回逆现象分析

目的探讨脑肿瘤干细胞（BTSCs）体外分化过程中的回逆现象，为研究其分化抑制机制奠定基础。方法利用CD133免疫磁珠筛选系统，从肿瘤组织中分离获得的CD133^＋细胞（BTSCs）分成4组进行培养：（1）含10％胎牛血清（FCS）；（2）10％FCS＋丙戊酸钠注射液（VPA）；（3）无FCS＋生长因子；（4）无FCS＋生长因子＋VPA。取不同时间点上的细胞，相差显微镜观察其形态变化：流式细胞术检测与分化相关的标志物、细胞周期和DNA倍体变化；利用免疫激光共聚焦分析与分化相关标志物的共表达情况。结果无FCS条件下培养的BTSCs呈悬浮球状生长，高表达CD133和巢蛋白（nestin），不表达胶质纤维酸性蛋白（GFAP）和β-微管蛋白Ⅲ（β-TubulinⅢ）。G0／G1期细胞占大多数，G2／M期细胞接近0％，DNA都是异倍体，对VPA反应不敏感。含FCS培养的原本悬浮的细胞约4h开始贴壁。均呈圆形。此后逐渐向多形性分化，至7d时分化的细胞部分又返回至圆形。至10d-21d时，有的还能重新恢复球形，并呈悬浮生长。培养3d、7d、10d和21d时，CD133、nestin阳性细胞数先降后升，GFAP^＋和β-TubulinⅢ^＋细胞数始终处于较低水平。含FCS培养液中加入VPA。细胞形态上未见上述的回逆现象，CD133和nestin表达的先降后升现象消失，GFAP和β-TubulinⅢ在第7天以后表达明显升高，但极大部分细胞共表达nestin。而神经干细胞（NSCs）在含FCS培养至10d时，即以GFAP和β-TubulinⅢ表达为主，未见CD133^＋细胞。此外，含血清培养时BTSCs仍以异倍体为主。含少量的G2／M期细胞，加VPA诱导后细胞周期和DNA倍体变化不明显。结论BTSCs在含血清条件下培养出现的多向分化表型不稳定，时有去分化所导致的回逆。加入诱导分化剂VPA培养，虽然能阻止回逆现象出现，并有代表星形胶质细胞和神经元标志物表达上升．但因其共表达nestin而仍属于未完全分化细胞，表明BTSCs分化始终处于受抑状态。     

细胞移植用于心肌修复--细胞心肌成形术的研究进展

由于心肌细胞的增殖能力很低，细胞移植作为一种新的治疗方法用于改善心功能及心肌活力已受到广泛的关注。目前已有胚胎干细胞、骨髓间充质干细胞和内皮干细胞在体外诱导分化为心肌细胞；动物实验中用于心肌移植的细胞有胚胎心肌细胞、造血干细胞、骨髓间充质干细胞、骨骼肌成肌细胞、内皮干细胞、肝干细胞和神经干细胞。其中成肌细胞移植用来改善心肌梗死后的心脏功能，已有临床报道，并取得成功。     

中药安迪对HL-60细胞分化的诱导作用

目的　探讨安迪粉针剂 (Andi)对 HL- 60细胞分化的诱导作用 .方法　采用人早幼粒白血病细胞株 (HL - 60 )为靶细胞 ,分为不加任何药物的对照组 (C组 )、安迪粉针剂 (Andi)组、阳性对照药维甲酸 (RA)组和苦参 (KS)组 ,进行体外培养和诱导分化 ,观测细胞生长曲线、细胞形态、硝基蓝四氮唑(NBT)还原和吞噬能力等指标 .结果　 2 mg· L-1 Andi可显著地抑制 HL - 60细胞增殖 ,使原始细胞分化为中幼以下的成熟细胞 ,分化后的细胞具有 NBT还原能力和吞噬功能 ;Andi为 68.0 % ,RA为 61 .5% ,KS为 59.0 % ,C组还原能力仅6.0 % (P<0 .0 1 vs C) .其形态的改变和吞噬能力与阳性对照药维甲酸 (RA)和苦参 (KS)相似 ,分别为 52 .0 % ,45.5%和56.5% (P>0 .0 5) ;均明显高于空白对照组 .C组吞噬功能仅7.5% (P <0 .0 1 vs C)其 NBT还原能力与 KS相当 (P >0 .0 5) .结论　 Andi对 HL - 60细胞具有显著的诱导分化作用     

Recurrent venous thrombosis following free flap surgery: The role of heparin-induced thrombocytopenia

Complications following free tissue transfer have been well established in the literature. Common and rare causes of free flap failure must be addressed by the treating surgeon when microvascular patency is threatened. With the evolution and prevalence of microsurgery, ‘rare’ causes of free flap failure will become increasingly frequent. A high index of suspicion must be established in patients with multiple failed operative interventions. A case of recurrent free flap failure secondary to heparin-induced thrombocytopenia is presented in a patient with a history of squamous cell carcinoma of the floor of the mouth, and a long-standing history of alcohol and tobacco consumption.