胆红素对新生豚鼠蜗核损伤的动态研究

目的　探讨听性脑干反应 (ABR)在胆红素所致听力损伤中的监测价值。方法　 2 0只新生 5日豚鼠随机分为胆红素组和对照组 ,胆红素组腹腔注射胆红素 (2 0 0 μg/ g) ,对照组腹腔注射相应体积的生理盐水 (0 1ml/g) ,于腹腔注射前和后 4h、 8h分别记录ABR并观察神经行为状况 ,每组各取 5只于腹腔注射后 4h、 8h分别行蜗核形态学观察。结果　注射胆红素后 ,ABR波潜伏期、波间期呈逐渐延长趋势 ,振幅呈逐渐下降趋势 ,其中波Ⅱ改变出现最早且最明显。用药后 4h ,无明显神经系统症状和形态学改变时 ,Ⅰ -Ⅱ波间期延长及波Ⅱ振幅下降与对照组及用药前比较差异有显著性 (P <0 0 1) ;用药后 8hABR改变更明显 ,同时伴有神经症状和镜下形态学改变 ,蜗核体积及大椭圆细胞截面积显著性减小 (P <0 0 1)。结论　ABR是监测胆红素所致听力损害早期的敏感指标 ,优于耳蜗电图和耳声发射等主要反映周围听觉系统功能状态的电生理技术 ,加强高胆红素血症新生儿的ABR动态监测有非常重要的临床意义     

胆汁中提取胆红素的最佳实验条件探讨

从胆汁中提取胆红素，所得成品，其含量和收率受许多因素的影响。本文通过正交表实验选择，结果表明，最主要因素为醇析酸化ｐＨ值，其次为乙醇浓度、乙醇用量和抗氧剂用量等。     

两种检测血清结合胆红素方法的结果比较

[目的]比较2，4-二氯苯胺重氮法（DCA）和钒酸盐氧化法检测血清结合胆红素的结果。[方法]根据NCCLS（EP6-P）评价方案，评估两种方法的相关性和偏倚。[结果]两种方法的检测结果相关性良好（r=0．9985），Ⅰ、Ⅱ、Ⅲ组检测结果无差异，Ⅳ组结果有差异。[结论]应建立与方法学相对应的血清结合胆红素参考值范围。     

生物标志物联合检测在脓毒症相关急性肝损伤患者中的应用价值

目的:探讨生物标志物联合检测对脓毒症相关急性肝损伤患者的早期诊断和预后评估的价值。方法:收集内蒙古科技大学包头医学院第一附属医院重症医学科(ICU)2019年11月-2022年1月收治的104例脓毒症患者临床资料,按照入ICU时是否发生急性肝损伤分为脓毒症相关急性肝损伤组(n=42)和非肝损伤组(n=62),将脓毒症相关急性肝损伤患者按28 d是否存活分为存活组(n=16)和死亡组(n=26),记录104例患者入ICU 6 h内血清正五聚蛋白3(PTX-3)、C-反应蛋白(CRP)、降钙素原(PCT)、总胆红素(TBIL)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、凝血酶原时间国际标准化比值(INR值)、凝血酶原时间(PT)、凝血酶原活动度(PTA)、血小板(PLT)、入ICU 24 h内急性生理与慢性健康状况评估量表II(APACHEII)评分最差值及序贯器官衰竭评估(SOFA)评分;同时记录脓毒症相关急性肝损伤患者28 d预后。PTX-3的相关性采用Spearman相关分析;绘制受试者工作特征曲线(ROC曲线),计算ROC曲线下面积(AUC),分析各指标单独检测以及联合检测对脓毒...     

脂联素、直接胆红素及甘油三酯对2型糖尿病患者大血管病变的诊断价值

目的 探讨脂联素（APN）、直接胆红素（DB）、甘油三酯（TG）及三者联合对 2 型糖尿病（T2DM）患者大血管病变的诊断价值。方法 选取120例T2DM患者,根据是否合并大血管病变将其分为T2DM组（62 例）和 T2DM+大血管病变组（58 例）。比较两组的临床资料;采用非条件多因素逐步Logistic回归分析T2DM患者合并大血管病变的风险因素;绘制受试者工作特征（ROC）曲线评估APN、DB、TG及三者联合对T2DM患者合并大血管病变的诊断效能。结果 非条件多因素逐步Logistic回归分析结果显示,■是T2DM患者合并大血管病变的危险因素（P <0.05);■是T2DM患者合并大血管病变的保护因素（P <0.05）。ROC曲线分析结果显示,当APN ≤ 5.870 nmol/mL时,诊断T2DM患者合并大血管病变的曲线下面积（AUC）为0.718,敏感性为70.7%(95% CI:0.571,0.815）,特异性为61.3%(95% CI:0.480,0.713）;当DB ≤ 14.205 mol/L 时,AUC 为 0.772,敏感性为 74.1% (95% C...     

Comparative study of pituitary and bacteria-derived human growth hormone by monoclonal antibodies

We have established hybridoma lines which secrete mouse monoclonal antibodies (Mabs) to human pituitary growth hormone, hGH. Using indirect competitive ELISA and indirect passive hemagglutination inhibition twelve different Mabs were characterized with regard to cross-reactivity with the hGH-related hormones, human chorionic somatomammotropin, hCS, and human prolactin, hPRL. The reactivity of these Mabs with pituitary hGH was compared to that with either bacterially-produced methionyl-hGH or to that of reduced and S-carboxymethylated hGH, which has an altered conformation. None of the Mabs reacted with hPRL. Four did not react with hCS whereas the others showed varying degree of cross-reactivity with hCS. All Mabs reacted more weakly with reduced and S-carboxymethylated hGH than with the native form of the hormone, which was not seen with conventional rabbit antisera to hGH. Thus in the case of hGH the Mabs are superior to conventional antisera in revealing small conformational differences. However the pituitary and bacterially-derived methionyl-hGH were indistinguishable as determined by the 12 Mabs.     

Demonstration of T4, T8, M1 and B7 determinants on human T cells with a rosette test: implications for the assay specificity of monoclonal antibodies

Under optimal test conditions significantly more freshly isolated human T cells reacted with OKT4, OKT8, OKM1 and OKB7 monoclonal antibodies (Mabs) in the indirect antiglobulin rosetting reaction (IARR) than by indirect immunofluorescence. Rabbit erythrocytes (E) coated with anti-mouse immunoglobulin were more sensitive indicator cells in the IARR than similarly coated sheep E. Treatment of T cells with neuraminidase further enhanced T cell reactivity in the IARR with each Mab so that an average of 60% or more of T cells were T4+, T8+ and M1+ and at least 40% had the T4+ T8+ phenotype. The various findings suggest that the rosette assay detects determinants on T cells that are expressed below the detection threshold of immunofluorescence. Moreover, these findings indicate that the cellular specificities of a particular Mab may change when one assay system is substituted for another or when the protocol of a particular assay is altered.     

Cells with dendritic morphology and bright interleukin-1 alpha staining circulate in the blood of patients with rheumatoid arthritis.

Freshly isolated peripheral blood mononuclear cells (PBMC) from 10 healthy volunteers, 28 patients with rheumatoid arthritis (RA), eight patients with osteoarthritis, and five patients with ankylosing spondylitis were examined for interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) production using monoclonal antibodies and an indirect immunofluorescent method. In freshly isolated PBMC from healthy controls very few cells were stained for either IL-1 type. All 20 RA patients who were not receiving parenteral gold therapy had PBMC staining for IL-1 alpha. In these patients, up to 7.5% of PBMC showed bright IL-1 alpha staining (range 1.2-7.5%). No IL-1 beta staining was seen. These IL-1 alpha-staining cells had a dendritic morphology and the percentage of cells staining correlated well with levels of C-reactive protein, an index of disease activity in these RA patients. Significantly fewer IL-1 alpha-staining cells were present in the peripheral blood of RA patients receiving gold therapy and in the blood of patients with osteoarthritis and ankylosing spondylitis. These IL-1 alpha-containing cells, circulating in the blood of RA patients and correlating with disease activity have not been previously described. These results support the idea that IL-1 alpha plays an important role in the pathogenesis of rheumatoid inflammation.     

Preparation of Aminated Macroporous Polyvinyl Alcohol Resins and Evaluation for Bilirubin Adsorption

In the present study we prepared macroporous polyvinyl alcohol beads. A series of bilirubin adsorbents were generated by immobilization of eight amine agents to the beads as ligands. The adsorption of bilirubin was evaluated by in vitro static and dynamic adsorption tests. The results show that these adsorbents have excellent adsorption efficiency and capacity. Among the eight ligands, trimethylamine (TMA), triethylamine (TEA) and 1,6- hexanediamine(HDA) showed the highest adsorption capacity. The adsorption equilibrium can be achieved in half an hour, and the adsorption percentage of bilirubin was up to 80%. Static electricity and hydrophobic interaction played the main role in bilirubin adsorption, and the adsorption was found to match the monolayer model. The excellent adsorption of these adsorbents indicates their potential in clinical treatment.     

Primary biliary cirrhosis and coronary atherosclerosis: Protective antioxidant effect of bilirubin

Increased concentration of lipid peroxidation products in patients with primary biliary cirrhosis is related to elevation of serum lipid content, but not to activation of lipid peroxidation. Hyperbilirubinemia in patients with primary biliary cirrhosis is accompanied by a decrease in the concentration of lipid peroxidation products and increase in antioxidant activity of blood serum. Antioxidants play a major role in the prevention of atherosclerosis. We hypothesized that the absence of increased risk of atherosclerosis in patients with primary biliary cirrhosis is due to inhibition of lipid peroxidation in blood serum by antioxidant compound bilirubin. __________ Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 145, No. 1, pp. 23–28, January, 2008