全文获取类型
收费全文 | 3123篇 |
免费 | 228篇 |
国内免费 | 73篇 |
专业分类
耳鼻咽喉 | 7篇 |
儿科学 | 216篇 |
妇产科学 | 43篇 |
基础医学 | 279篇 |
口腔科学 | 87篇 |
临床医学 | 470篇 |
内科学 | 475篇 |
皮肤病学 | 46篇 |
神经病学 | 108篇 |
特种医学 | 80篇 |
外科学 | 190篇 |
综合类 | 569篇 |
预防医学 | 318篇 |
眼科学 | 68篇 |
药学 | 322篇 |
5篇 | |
中国医学 | 92篇 |
肿瘤学 | 49篇 |
出版年
2024年 | 7篇 |
2023年 | 60篇 |
2022年 | 93篇 |
2021年 | 117篇 |
2020年 | 113篇 |
2019年 | 99篇 |
2018年 | 91篇 |
2017年 | 109篇 |
2016年 | 125篇 |
2015年 | 101篇 |
2014年 | 164篇 |
2013年 | 189篇 |
2012年 | 165篇 |
2011年 | 180篇 |
2010年 | 137篇 |
2009年 | 143篇 |
2008年 | 152篇 |
2007年 | 131篇 |
2006年 | 107篇 |
2005年 | 110篇 |
2004年 | 118篇 |
2003年 | 93篇 |
2002年 | 79篇 |
2001年 | 89篇 |
2000年 | 72篇 |
1999年 | 42篇 |
1998年 | 38篇 |
1997年 | 43篇 |
1996年 | 43篇 |
1995年 | 34篇 |
1994年 | 33篇 |
1993年 | 24篇 |
1992年 | 32篇 |
1991年 | 31篇 |
1990年 | 19篇 |
1989年 | 29篇 |
1988年 | 20篇 |
1987年 | 29篇 |
1986年 | 15篇 |
1985年 | 35篇 |
1984年 | 19篇 |
1983年 | 19篇 |
1982年 | 19篇 |
1981年 | 12篇 |
1980年 | 11篇 |
1978年 | 5篇 |
1976年 | 7篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1972年 | 4篇 |
排序方式: 共有3424条查询结果,搜索用时 15 毫秒
11.
12.
新型壳聚糖对血浆胆红素和细胞因子吸附性能的体外筛选初步研究 总被引:7,自引:0,他引:7
目的观察各类新型吸附剂对重型肝炎患者血浆中的胆红素和细胞因子的吸附性能。方法第一步,收集第一组重型肝炎患者的血浆各3ml,以8种不同的吸附剂(1#~3#为致孔剂浓度分别为3%、1%、5%的壳聚糖,4#为胺基化壳聚糖,5#为苯乙烯/二乙烯苯聚合物,6#为后交连苯乙烯/二乙烯苯聚合物,7#为壳聚糖-苯乙烯/二乙烯苯聚合物,8#为壳聚糖-后交连苯乙烯/二乙烯苯聚合物)各1ml进行吸附,检测对胆红素的吸附能力,做吸附剂筛选实验和吸附实验。第二步,用以上筛选出吸附率较好的两种吸附剂各1ml对第二组患者血浆(各3ml)进行胆红素和细胞因子的吸附实验。结果第一步实验显示胺基壳聚糖(4#)和苯乙烯/二乙烯苯聚合物(5#)有较好的吸附效果,4#吸附剂对总胆红素(TBiL)、直接胆红素(DBiL)、间接胆红素(IBiL)的吸附率分别为50.5%±3.4%、57.0%±11.3%、39.0%±7.2%;5#分别为30.1%±2.5%、32.6%±3.0%、27.6%±2.9%。第二步实验显示4#胺基化壳聚糖吸附后血浆中TBiL、DBiL、IBiL、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)水平下降比5#明显,有统计学意义(P<0.001)。结论胺基化壳聚糖(4#)体外吸附重型肝炎患者血浆中胆红素、细胞因子效果有明显优势。 相似文献
13.
胆红素对新生豚鼠蜗核损伤的动态研究 总被引:2,自引:0,他引:2
目的 探讨听性脑干反应 (ABR)在胆红素所致听力损伤中的监测价值。方法 2 0只新生 5日豚鼠随机分为胆红素组和对照组 ,胆红素组腹腔注射胆红素 (2 0 0 μg/ g) ,对照组腹腔注射相应体积的生理盐水 (0 1ml/g) ,于腹腔注射前和后 4h、 8h分别记录ABR并观察神经行为状况 ,每组各取 5只于腹腔注射后 4h、 8h分别行蜗核形态学观察。结果 注射胆红素后 ,ABR波潜伏期、波间期呈逐渐延长趋势 ,振幅呈逐渐下降趋势 ,其中波Ⅱ改变出现最早且最明显。用药后 4h ,无明显神经系统症状和形态学改变时 ,Ⅰ -Ⅱ波间期延长及波Ⅱ振幅下降与对照组及用药前比较差异有显著性 (P <0 0 1) ;用药后 8hABR改变更明显 ,同时伴有神经症状和镜下形态学改变 ,蜗核体积及大椭圆细胞截面积显著性减小 (P <0 0 1)。结论 ABR是监测胆红素所致听力损害早期的敏感指标 ,优于耳蜗电图和耳声发射等主要反映周围听觉系统功能状态的电生理技术 ,加强高胆红素血症新生儿的ABR动态监测有非常重要的临床意义 相似文献
14.
从胆汁中提取胆红素,所得成品,其含量和收率受许多因素的影响。本文通过正交表实验选择,结果表明,最主要因素为醇析酸化pH值,其次为乙醇浓度、乙醇用量和抗氧剂用量等。 相似文献
15.
[目的]比较2,4-二氯苯胺重氮法(DCA)和钒酸盐氧化法检测血清结合胆红素的结果。[方法]根据NCCLS(EP6-P)评价方案,评估两种方法的相关性和偏倚。[结果]两种方法的检测结果相关性良好(r=0.9985),Ⅰ、Ⅱ、Ⅲ组检测结果无差异,Ⅳ组结果有差异。[结论]应建立与方法学相对应的血清结合胆红素参考值范围。 相似文献
16.
Comparative study of pituitary and bacteria-derived human growth hormone by monoclonal antibodies 总被引:1,自引:0,他引:1
We have established hybridoma lines which secrete mouse monoclonal antibodies (Mabs) to human pituitary growth hormone, hGH. Using indirect competitive ELISA and indirect passive hemagglutination inhibition twelve different Mabs were characterized with regard to cross-reactivity with the hGH-related hormones, human chorionic somatomammotropin, hCS, and human prolactin, hPRL. The reactivity of these Mabs with pituitary hGH was compared to that with either bacterially-produced methionyl-hGH or to that of reduced and S-carboxymethylated hGH, which has an altered conformation. None of the Mabs reacted with hPRL. Four did not react with hCS whereas the others showed varying degree of cross-reactivity with hCS. All Mabs reacted more weakly with reduced and S-carboxymethylated hGH than with the native form of the hormone, which was not seen with conventional rabbit antisera to hGH. Thus in the case of hGH the Mabs are superior to conventional antisera in revealing small conformational differences. However the pituitary and bacterially-derived methionyl-hGH were indistinguishable as determined by the 12 Mabs. 相似文献
17.
D G Haegert 《Journal of immunological methods》1985,82(2):261-266
Under optimal test conditions significantly more freshly isolated human T cells reacted with OKT4, OKT8, OKM1 and OKB7 monoclonal antibodies (Mabs) in the indirect antiglobulin rosetting reaction (IARR) than by indirect immunofluorescence. Rabbit erythrocytes (E) coated with anti-mouse immunoglobulin were more sensitive indicator cells in the IARR than similarly coated sheep E. Treatment of T cells with neuraminidase further enhanced T cell reactivity in the IARR with each Mab so that an average of 60% or more of T cells were T4+, T8+ and M1+ and at least 40% had the T4+ T8+ phenotype. The various findings suggest that the rosette assay detects determinants on T cells that are expressed below the detection threshold of immunofluorescence. Moreover, these findings indicate that the cellular specificities of a particular Mab may change when one assay system is substituted for another or when the protocol of a particular assay is altered. 相似文献
18.
Preparation of Aminated Macroporous Polyvinyl Alcohol Resins and Evaluation for Bilirubin Adsorption
WANG Wei-chao ZHANG Sheng-nan HU Yue-han XIE Hui OU Lai-liang YU Yao-ting KONG De-ling GU Han-qing 《中国生物医学工程学报(英文版)》2008,17(3)
In the present study we prepared macroporous polyvinyl alcohol beads. A series of bilirubin adsorbents were generated by immobilization of eight amine agents to the beads as ligands. The adsorption of bilirubin was evaluated by in vitro static and dynamic adsorption tests. The results show that these adsorbents have excellent adsorption efficiency and capacity. Among the eight ligands, trimethylamine (TMA), triethylamine (TEA) and 1,6- hexanediamine(HDA) showed the highest adsorption capacity. The adsorption equilibrium can be achieved in half an hour, and the adsorption percentage of bilirubin was up to 80%. Static electricity and hydrophobic interaction played the main role in bilirubin adsorption, and the adsorption was found to match the monolayer model. The excellent adsorption of these adsorbents indicates their potential in clinical treatment. 相似文献
19.
Primary biliary cirrhosis and coronary atherosclerosis: Protective antioxidant effect of bilirubin 总被引:1,自引:0,他引:1
L. B. Dudnik O. A. Azyzova N. P. Solovyova A. P. Savchenkova M. A. Pokrovskaya 《Bulletin of experimental biology and medicine》2008,145(1):18-22
Increased concentration of lipid peroxidation products in patients with primary biliary cirrhosis is related to elevation
of serum lipid content, but not to activation of lipid peroxidation. Hyperbilirubinemia in patients with primary biliary cirrhosis
is accompanied by a decrease in the concentration of lipid peroxidation products and increase in antioxidant activity of blood
serum. Antioxidants play a major role in the prevention of atherosclerosis. We hypothesized that the absence of increased
risk of atherosclerosis in patients with primary biliary cirrhosis is due to inhibition of lipid peroxidation in blood serum
by antioxidant compound bilirubin.
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 145, No. 1, pp. 23–28, January, 2008 相似文献
20.
本文采用间接ELISA法检测了11株抗B-CLL患者IgM腹水和杂交瘤上清McAb的相对亲和力。结果提示,各株McAb结合抗原的相对亲和力不同。根据50%最大结合浓度分为两组。其中4株杂交瘤上清McAb与纯化腹水McAb测得结果基本吻合。实验结果为合理地选用这些单抗提供了重要依据。 相似文献