A clean-air incubator for the cultivation and manipulation of cells at a constant temperature

Summary An incubator is described in which heated air is circulated through a high efficiency particulate air filter. The incubator is intended for the long term cultivation of cells and tissues, with culture manipulations performed in a microbe-free environment and without changes in temperature.     

Some techniques for portable incubators

A fully satisfactory portable infant incubator has not yet been marketed. This paper discusses some improvements which have been tried experimentally. These include better heat delivery, faster response to the infant's needs, higher humidity, lower power consumption, and wider range of oxygen content.     

人工模拟不同外邪环境对正常及免疫低下小鼠一般情况的影响

目的 探讨不同环境因素对正常及免疫低下小鼠一般情况的影响.方法 选取64只昆明种小鼠随机分为正常组、风寒组、寒湿组、湿热组、低免组、风寒低免组、寒湿低免组、湿热低免组,每组8只.腹腔注射环磷酰胺80mg/(kg·d)连续3天制备低免模型,借助人工气候箱制备风寒、寒湿、湿热外邪环境模型.于造模前、后观察记录小鼠精神活动、毛色、进食量、饮水量、体重、皮肤、关节及大便情况,连续观察7天.结果 与正常组比较,各模型组小鼠进食量、饮水量均明显减少,体重增长明显减慢(P＜0.05或P＜0.01).与低免组比较,寒湿低免组小鼠进食量减少、湿热低免组的饮水量减少,且两组体重都较造模前减轻(P＜0.05).外邪低免组与相应的外邪组小鼠比较,各外邪低免组进食量、饮水量明显减少,体重减轻(P＜0.05或P＜0.01).各模型组小鼠在精神状态、活动、毛色、皮肤、关节及大便等方面均有一定影响,而外邪低免组表现明显.结论 3种环境因素对正常及免疫低下小鼠的一般情况都有影响,对免疫低下小鼠的影响更为明显.     

婴儿培养箱风险管理初探

通过介绍婴儿培养箱的工作原理、安全使用注意事项,探讨婴儿培养箱常见不良事件的产生原因,分析其风险管理要点.     

基于T/CAME37-2021团体标准的婴儿培养箱运行性能参数实时监测

目的:依据国家卫生部关于《婴儿培养箱温度和湿度实时监测系统技术要求》(T/CAME37-2021)团体标准,研制婴儿培养箱温度和相对湿度实时监测系统,对婴儿培养箱运行性能参数实时监测与分析,提升婴儿培养箱使用的安全管理.方法:软件设计从采集→显示→传输→展示及分析整个过程的功能出发,将感知层、传输层和应用层3层功能串联...     

Synchrony of the first division as an index of the blastocyst formation rate during embryonic development

Purpose

To devise an uninvasive selection system for human embryos with high developmental potential after a single oocyte retrieval cycle by comparing the in vitro and in vivo effectiveness of first division synchrony against subsequent embryonic developmental stages.

Methods

The effects of using assisted reproductive technology on 948 embryos that were produced in 137 cycles were examined by dividing the embryos into “early cleavage” (first division within 25.90 hours) and “late cleavage” (first division at or after 25.90 hours) groups and comparing the blastocysts and good‐quality blastocyst formation rates between the two groups. These two groups were each divided further into “high synchrony” (first division synchrony within 3.96 hours) and “low synchrony” (first division synchrony at or after 3.96 hours) groups. The blastocysts, good‐quality blastocyst formation rates, and pregnancy rates were compared among these four groups.

Results

Both the blastocysts and good‐quality blastocyst formation rates were significantly higher in the early‐cleavage groups than in the late‐cleavage groups. The blastocyst formation rate of the latter was also significantly increased in the high‐synchrony, compared with the low‐synchrony, group.

Conclusion

First division synchrony in a single oocyte retrieval cycle could be a useful assessment of the blastocyst formation rate that enables the selection of viable embryos at an early stage of culture.     

婴儿培养箱监控系统综述

婴儿培养箱监控系统的作用为收集和分析培养箱中的被测参数,监控、评估其性能及早产儿的健康状态.一个高效的监控系统对确保培养箱的安全运行及监控早产儿的健康状态至关重要.该文介绍了婴儿培养箱监控系统的组成、各组成部分的研究现状和发展趋势,为研发出更加高效好用又符合性能需求的婴儿培养箱监控系统提供了参考.     

婴儿培养箱中央监测系统研制

目的设计一套可以对婴儿培养箱实时状态进行无线监测的系统。方法利用短距离无线通信技术为载体,采用STC89C52为控制核心,实现从机和主机之间的信息传递。结果实现了各个培养箱的温度信息能实时准确地传达到放置在护理站的主机系统。结论经仿真测试,该系统基本满足了设计要求,其设计思想可以为其他医疗设备的监测提供理论参考。     

早产儿双面蓝光治疗时体温及箱温调控方式探讨

目的 探讨早产儿高胆红素血症暖箱内双面蓝光治疗时体温变化及箱温调控方式,以维持病儿蓝光治疗期间所需体温的恒定水平.方法 选取2014年8月至2015年8月衢州市人民医院收治的高胆红素血症早产儿80例,随机将其分成观察组和对照组,观察组在蓝光治疗前测量体温1次,当体温<36.5℃时降低暖箱温度1.5℃;体温在36.5℃~37.5℃,降低箱温2.0℃;对照组在蓝光治疗前测体温<36.5℃,降低箱温0.5℃,体温在36.5℃~37.5℃之间,降低箱温1℃,观察两组在蓝光治疗期间体温状况.结果 观察组体温上升幅度为(37.0±0.3)℃,明显低于对照组的(37.5±0.3)℃,差异具有统计学意义(t=7.96,P<0.05);观察组发热的发生率为12.5%,低于对照组的62.5%,差异具有统计学意义(χ2=22.78,P<0.05).结论 早产儿在进行双面蓝光治疗时,根据蓝光治疗前测量的体温调控适宜的箱温,可有效降低早产儿光疗时发热的发生率.     

新型便携二氧化氯汽化消毒装置在 婴儿培养箱终末消毒的应用

〔摘 要〕 目的：探究新型便携二氧化氯汽化消毒装置相较于常规的床单位臭氧消毒在婴儿培养箱终末消毒的效果。方法：选取 2021 年 8 月至 2021 年 9 月在深圳市中西医结合医院新生儿科普通婴儿出院后待终末消毒的婴儿培养箱共 100 个,随机分为观察组与对照组,各 50 个。对照组采用常规臭氧消毒法,观察组采用新型便携二氧化氯汽化消毒 法,比较两种方法的消毒效果。结果：观察组空气细菌检测合格率为 98.00 %,高于对照组的 84.00 %,差异具有统 计学意义（P ＜ 0.05）。观察组物体表面细菌检测合格率为 100.00 %,高于对照组的 88.00 %,差异具有统计学意义 （P ＜ 0.05）。观察组空气细菌检出率为 34.00 %,低于对照组的 90.00 %,差异具有统计学意义（P ＜ 0.05）。观察 组物体表面细菌检出率为 42.00 %,低于对照组的 86.00 %,差异具有统计学意义（P ＜ 0.05）。结论：相较于常规 臭氧消毒法,新型便捷二氧化氯汽化消毒装置应用于婴儿培养箱的终末消毒,具有消毒效果较好且便携移动的优点。