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91.
血清乙型肝炎病毒标志物不同表现模式及肝功能异常对肾移植受者存活率的影响 总被引:1,自引:0,他引:1
目的 探讨血清乙型肝炎病毒标志物不同表现模式对肾移植受者长期存活的影响。方法 对 62例血清乙型肝炎病毒标志物阳性者及 1 96例血清乙型肝炎病毒标志物全阴性者肾移植术后的肝功能、人肾均存活的存活率等指标进行随访和回顾性分析。结果 术后早期血清乙型肝炎病毒标志物阳性组与血清乙型肝炎病毒标志物阴性组比较 ,肝功能异常发生率的差异无显著性 (P >0 .0 5) ;术后中远期 ,HBsAg、HBeAg及抗 HBc阳性者的肝功能受损率明显高于血清乙型肝炎病毒标志物阴性组及HBsAg、抗 HBe、抗 HBc阳性者 (P <0 .0 5) ,其人肾均存活的存活率也最低 (P <0 .0 5)。结论 对HBsAg、抗 HBe及抗 HBc阳性者进行肾移植应慎重 ,而HBsAg、HBeAg及抗 HBc阳性者则不适宜接受肾移植 相似文献
92.
93.
Akitoshi Yamada Yoshitaka Takeda Satoru Hayashi Kazuta Shimizu 《The Japanese Journal of Thoracic and Cardiovascular Surgery》2003,51(9):456-458
We experienced a case of familial spontaneous pneumothorax in three generations. Six of 13 family members had episodes of
spontaneous pneumothorax. It is well established that there are some diseases associated with human leukocyte antigen (HLA).
We performed HLA phenotyping for HLA of A, B and C. In our study, we detected the HLA haplotype A2, B61 in three of 4 who
had episodes of spontaneous pneumothorax. The HLA haplotype A2, B70 were also detected in three of 4 who had episodes. This
suggests that familial spontaneous pneumothorax might have hereditary factors. 相似文献
94.
Fumonisins as a possible contributory risk factor for primary liver cancer: A 3-year study of corn harvested in Haimen, China, by HPLC and ELISA 总被引:13,自引:0,他引:13
Y. Ueno K. Iijima S.-D. Wang Y. Sugiura M. Sekijima T. Tanaka C. Chen S.-Z. Yu 《Food and chemical toxicology》1997,35(12):1143-1150
Employing HPLC fluorometry, gas-liquid chromatography (GLC) and a novel enzymelinked immunosorbent assay (ELISA) based on a monoclonal antibody, 40 corn samples, each collected in 1993 from agricultural stocks for human consumption in Haimen (Jiangsu County) and Penlai (Shandong Province), high- and low-risk areas for primary liver cancer (PLC) in China, respectively, were analysed for fumonisins (FBs), aflatoxins (AFs) and trichothecenes. Levels and positive rates of FBs and deoxynivalenol (DON) were significantly higher in Haimen than in Penlai. ELISA of the 40 corn samples harvested in the two areas in 1994 revealed that FB contamination levels and rates in these areas were comparable to those observed in 1993 in Haimen. ELISA analysis of 1993 and 1994 products revealed a wide occurrence of AFB1 but the positive rates as well as levels were not significantly different between these areas. ELISA of the same sample number of corn harvested in 1995 revealed that FB contamination in Haimen was significantly higher than in Penlai. These 3-yearly surveys of corn samples (240 in total) demonstrated that corn harvested in Haimen was highly contaminated with FBs and that the contamination level, as well as positive rate in 1993 and 1995, were 10–50-fold higher than those in Penlai, suggesting FBs as a risk factor for promotion of PLC in endemic areas, along with the trichothecene DON. Co-contamination with AFs, potent hepatocarcinogens, was assumed to play an important role in the initiation of hepatocarcinogenesis. 相似文献
95.
用人喉癌手术切除标本建立了裸鼠移植瘤模型,已传至13代。原代移植成功率为66.7%,潜伏期30~70d;鼠间传代移植成功率为100%,潜伏期14~19d,生长稳定。经光学显微镜检查,各代移植瘤组织结构与原人喉癌组织基本一致。电镜检查证明,具有人喉癌特征,癌细胞间有大量桥粒,细胞浆中可见张力原纤维,有的张力原纤维与桥粒相连。细胞表面有较大的指状突,核膜较规则,胞浆中线粒体较多。 相似文献
96.
以地高辛甙元随机引物法标记HBV-DNA探针,以此探针检测慢性乙型肝炎患者的血清、肝组织,同时以ELISA法检测血清HBeAg、HBcAb。结果:血清NBeAg阳性率27%(10/37),血清HBV-DNA检出率57.1%(20/35),两者有显著性差异。血清HBcAb阳性率78.4%(29/37),肝组织HBV-DNA检出率83.8%(31/37),两者无显著性差异。血清与肝组织HBV-DNA检出率有显著性差异。提示:血清HBV-DNA检测是较HBeAg更为准确客观反映血液带毒状况的指标。而准确反映肝脏带毒状况的指标是肝组织HBV-DNA检测。当HBeAg阴转,血清HBV-DNA阴性而肝组织HBV-DNA阳性时,需注意肝硬化及肝癌的发生。 相似文献
97.
Pamela E. B. Rodgers-Johnson Frederick W. Hickling Aggrey Irons Bruce K. Johnson Maureen Irons-Morgan Gary A. Stone Clarence J. Gibbs 《Journal of molecular neuroscience : MN》1996,28(1-3):237-243
Reports of an 18-fold higher incidence of schizophrenia among second-generation Afro-Caribbeans, and especially Jamaican migrants
in the United Kingdom were soon called “an epidemic of schizophrenia,” with the inference that a novel virus, likely to be
perinatally transmitted, was a possible etiological agent. This intriguing observation led us to explore a possible link with
human T-cell lymphotropic virus type one (HTLV-I), because it is a virus that is endemic in the Caribbean Islands, is perinatally
transmitted, known to be neuropathogenic, and the cause of a chronic myelopathy (tropical spastic paraparesis/ HTLV-I associated
myelopathy). We therefore examined inpatients at the Bellevue Mental Hospital, Kingston, Jamaica and did standard serological
tests for retroviruses HTLV-I and HTLV-II and HIV-I and HIV-II on 201 inpatients who fulfilled ICD-9 and DSM III-R criteria
for schizophrenia. Our results produced important negative data, since the seropositivity rates for HTLV-I, the most likely
pathogen, were no greater than the seropositivity range for HTLV-I carriers in this island population, indicating that HTLV-I
and the other retroviruses tested do not play a primary etiological role in Jamaican schizophrenics. 相似文献
98.
Masanobu Satake Manabu Inuzuka Katsuya Shigesada Tsuneyuki Oikawa Yoshiaki Ito 《Cancer science》1992,83(7):714-722
The core sequence of the enhancer of murine leukemia virus (MuLV) long terminal repeat is highly conserved in a large number of MuLV strains and appears to play an essential role when SL3-3 or Moloney strains induce T cell lymphoma in mice. We found by using the electrophoretic mobility shift assay that a polyomavirus enhancer core-binding protein, PEBP2, bound to this core motif of MuLV. We also noted that PEBP2 in several hematopoietic cell lines derived from B lymphocyte, macrophage and myelocyte lineages migrated significantly faster than the authentic PEBP2 detected in NIH3T3 (ibroblasts. Interestingly, PEBP2 detected in the cell lines of T lymphocyte lineage appeared to contain both types, which were indistinguishable in electrophoretic mobility from those of NIH3T3 and of B lymphocyte, macrophage and myelocyte lineages. The treatment of the nuclear extract containing PEBP2 with phosphatase generated PEBP3, which is a subcomponent of PEBP2 and retained the same DNA-binding specificity as PEBP2. The altered mobility of hematopoietic cell-derived or T lymphocyte-derived PEBP2 was found to be due to the alteration of the mobility of PEBP3. Based on the distinct mobility of PEBP2/3 of T lymphocytes from those of other hematopoietic cells, we discuss the implication of PEBP2 in MuLV-induced T cell leukemia and T cell-specific gene expression. 相似文献
99.
Iain L. Campbell 《Journal of neuroimmunology》1996,71(1-2)
To elucidate the possible involvement of the inducible nitric oxide synthase (iNOS) and NO in the development of lymphocytic choriomeningitis (LCM), the consequences of inhibition of iNOS by the inhibitor aminoguanidine was examined in mice following intracerebral infection with LCM virus (LCMV). Aminoguanidine administration to mice infected with LCMV completely blocked increased plasma nitrate/nitrite levels and led to increased proinflammatory cytokine gene expression at early stages of lesion development in the brain, enhanced clinical severity and decreased survival time. The levels of LCMV recovered from the brain of aminoguanidine treated mice did not differ from those in infected control mice. These findings argue against either an anti-viral or pathogenic role of NO in LCM but rather suggest a possible protective action of this mediator. 相似文献
100.
Mary Steidl Matsui Isabella Illarda Nianci Wang Vincent A. DeLeo 《Experimental dermatology》1993,2(6):247-256
Abstract Several lines of evidence implicate protein kinase C (PKC) in the development of basal cell and squamous cell carcinomas, tumors which originate from epidermal keratinocytes. To examine PKC in a model relevant to human skin, we exposed normal human epidermal keratinocytes (NHEK) in serum-free media to a variety of PKC agonists and antagonists. NHEK PKC activity increased up to 10-fold within the 1st hour of exposure to tetradecanoyl phorbol acetate (TPA), and gradually returned to control values within 72 h. TPA-induced PKC activity was enhanced by pretreatment of cultures with protein and RNA synthesis inhibitors. TPA-induced growth arrest and differentiation was antagonized by staurosporine. Down-regulation by bryostatin pretreatment blocked TPA-stimulated differentiation. Our overall conclusion is that activation of PKC in cultured human keratinocytes is required for differentiation. These results are crucial to the analysis of compounds suspected of promoting or inhibiting epidermal tumors. 相似文献