依那普利对实验性心肌梗死大鼠左室gp130基因表达的影响

目的 :研究急性心肌梗死 (AMI)后左室重塑 (LVRM)的发生与gp130基因异常表达的关系及依那普利对gp130表达的影响。方法 :AMI后 2 4h存活的 73只雄性Wistar大鼠随机分为AMI组及依那普利治疗组 ,AMI组又依照术后 1、3、7、14、2 1、2 8d分为 6个时间点 ,另设假手术组及正常对照组。各 8只。依那普利组于术后第 2天起灌胃给药 (10mg·kg-1·d-1) ,连续 4周。行心脏标本病理分析 ,分别采用放射免疫法和氯氨T法测定左室非梗死区 (LVNIZ)血管紧张素Ⅱ (AngⅡ )和LVNIZ羟脯氨酸含量 (HC) ,用RT PCR法检测LVNIZgp130mRNA的表达。最终获完整资料大鼠 76只 ,于上述各组分别为 9、8、8、9、9、8、9、8和 8只。结果 :①与正常及假手术组相比 ,AMI组LVRM参数 :心脏重量 (HW )、左室重量 (LVW )、左室重量指数 (LVWI)、心肌细胞横径 (TDM)、HC均显著增加(P <0 .0 5～ 0 .0 1) ;②AMI组LVNIZAngⅡ明显升高 ,与LVRM相关性好 (P <0 .0 1) ;③LVNIZgp130mRNA表达于AMI第 1天即开始明显增加 ,第 7天达高峰 ,以后有所下降 ,但第 2 8天时仍明显高于对照组 (P <0 .0 5～ 0 .0 1) ;④相关分析显示LVNIZgp130mRNA表达水平与LVNIZAngⅡ、LVRM参数间呈正相关 (P <0 .0 5～ 0 .0 1) ;⑤与AMI第 2 8天组相比 ,依那普利组LVRM明显减轻     

小鼠对HIV-2 gp105核酸疫苗免疫应答的研究

目的: 探讨HIV- 2gp105基因核酸疫苗在小鼠体内的免疫应答, 为开发HIV- 2核酸疫苗提供实验依据。方法:将HIV- 2外膜蛋白 (gp105 )基因插入真核表达质粒载体pVAX1中, 构建pVAX1 gp105重组表达质粒。将其肌注免疫BALB/c小鼠, 用ELISA法检测小鼠血清抗HIV -2抗体, 用流式细胞仪测定CD4 、CD8 T细胞亚群数, 以乳酸脱氢霉释放法检测脾特异性CTL的杀伤活性。结果: 重组质粒pVAX1 -gp105免疫组小鼠的血清抗体滴度、脾T细胞亚群的数量及特异性CTL的杀伤活性, 均明显高于对照组, 分别为P<0. 01, P<0. 05和P<0. 01。结论: HIV -2gp105核酸疫苗能诱导小鼠产生特异性细胞和体液免疫。     

Detection of orientation-specific anti-gp120 antibodies by a new N-glycanase protection assay

Several functions have been assigned to the extensive glycosylation of HIV-1 envelope glycoprotein gp120, especially immune escape mechanisms, but the intramolecular interactions between gp120 and its carbohydrate complement are not well understood. To analyse this phenomenon we established a new microwell deglycosylation assay for determining N-linked glycan accessibility after binding of gp120-specific agents. Orientation-specific exposition of gp120 in ELISA microplates was achieved by catching with either anti-C5 antibody D7324 or anti-V3 antibody NEA-9205. We found that soluble CD4 inhibited the deglycosylation of gp120 only when gp120 was caught by D7324 and not by NEA9205. In contrast, antibodies from HIV-infected individuals inhibited the deglycosylation best when gp120 was caught by NEA9205. These results demonstrated that both the CD4-binding site and the epitopes recognised by antibodies from HIV-infected individuals have N-glycans in the close vicinity. However, the difference in gp120 orientation indicates that antibodies in HIV-infected individuals, at least partly, bind to epitopes different from the CD4-binding site. Finally, we determined the structural class of the glycan of one V1 glycosylation site of prototype HIV-1 LAI gp120, which remained unsolved from previous studies, and found that it belonged to the complex type of glycans.     

The evolution of Epstein-Barr virus inferred from the conservation and mutation of the virus glycoprotein gp350/220 gene

To study variations of Epstein-Barr virus (EBV), we analyzed the gp350/220 gene for several cell lines and Japanese wild isolates using direct sequencing. The N-terminal region was highly conserved in all EBVs except for Jijoye/P3HR-1 and a few isolates. The variation of the region coincided with EBV types A and B (also referred to as types 1 and 2) and were, respectively, designated as the types a and b. The type A/a was detected in most Japanese cell lines and wild isolates, and was classified as China1 type with latent membrane protein (LMP) 1 gene. The type B/b was detected in only a few wild isolates with the Med and China2 types. The C-terminus had more diversity than the N-terminus and lacked the divergence between types A/a and B/b. The phylogenetic analyses of the gp350/220 and LMP1 genes may suggest a mode of EBV evolution into types A/a and B/b and then to LMP1 subtypes.     

福州"120"院前急救系统2 341例反应时间与处理时间的余弦模型分析

"120"急救系统每天不同时间接到呼叫后,即以最快速度派出急救车,处理后返院.本文将这2段时间分别称为"反应时间"(呼叫→出动)与"处理时间"(派出→返回).为研究这2段时间呼叫分布的规律,为制定应急措施提供参考,现将其拟合余弦模型进行分析,并报告如下.     

The relationship between fetal heart rate accelerations, fetal movements, and uterine contractions

The association between fetal heart rate (FHR) accelerations and fetal movements during uterine contractions was studied in 52 pregnant women near term or at the beginning of labor. FHR and uterine contractions were recorded by tococardiograph. At the same time, fetal movements, whether associated or not with contractions, were viewed by real-time ultrasound. During uterine contractions, 95.5% of the FHR accelerations were associated with fetal movements. Also, 90.9% of the accelerations which appeared when the uterus was not contracting were associated with fetal movements. Fetal movements were not seen in 91% of uterine contractions which were not associated with FHR accelerations. The suggestion is made that uterine contractions stimulate both fetal movements and FHR accelerations.     

恶性淋巴瘤mdr1和MRP mRNA及 P-gp表达水平与化疗疗效的相关研究

目的探讨恶性淋巴瘤mdr1、MRP mRNA和P-gp表达水平与化疗疗效的相关性.方法应用半定量逆转录多聚酶链反应(RT-PCR)技术和流式细胞术(FCM)方法,以8例人正常淋巴结为正常对照,对46例淋巴瘤患者[23例初治(HD1例,NHL22例)及23例复发(HD5例,NHL18例)]的mdr1 mRNA、MRP mRNA和P-gp表达水平与化疗疗效间的关系进行了前瞻性研究.结果复发患者mdr1基因和P-gp表达水平及阳性率均高于初治患者(P<0.001),而MRP基因表达水平及阳性率在复发与初治患者间差异无显著意义(P>0.05).mdr1基因及P-gp表达阳性患者的化疗有效(CR+PR)率(33.33%和26.67%)明显低于mdr1基因及P-gp表达阴性患者(85.71%和83.87%,P<0.001),而MRP基因表达阳性患者与阴性患者的化疗有效率差异无显著意义(P>0.05).相关分析显示,mdr1基因和P-gp表达水平之间有明显相关性(r=0.296 3,P<0.05),而mdr1和MRP之间、MRP与P-gp之间均无明显相关性(r=0.072 3,P>0.05;r=0.081 8,P>0.05).结论 mdr1基因及P-gp表达是恶性淋巴瘤患者多药耐药的主要机制,而MRP基因不是产生耐药的主要机制.mdr1基因及P-gp表达水平的高低与恶性淋巴瘤化疗疗效密切相关,而MRP基因的表达与化疗疗效未见相关.     

E-cadherin、β-catenin和p120ctn在所谓肺硬化性血管瘤中的表达

背景与目的所谓肺硬化性血管瘤(so-called pulmonary sclerosing hemangioma,PSH)是一种迄今未能确定其组织来源及性质的少见肺部肿瘤,多年来一直是人们研究的热点.本研究通过检测E-cadherin、β-catenin和p120ctn在PSH表面立方细胞和间质多角形细胞的免疫表型以探讨其组织来源.方法采用S P免疫组化法检测25例手术切除PSH标本及8例肺炎性假瘤标本的E-cadherin、β-catenin和p120ctn表达情况.结果25例PSH的立方细胞中,三种上皮粘附分子E-cadherin、β-catenin和p120 ctn均呈胞膜强阳性表达,β-catenin在胞膜强阳性表达的同时有胞质表达.而在多角形细胞中,E-cadherin表达缺失,β-catenin胞膜表达缺失伴部分胞质表达,p120 ctn胞质表达为主伴少量胞膜表达;三种粘附分子在多角形细胞中的表达存在异质性.血管瘤样区的腔内衬细胞E-cadherin、β-catenin和p120 ctn均呈胞质胞膜阳性表达.8例肺炎性假瘤中增生的Ⅱ型肺泡细胞E-cadherin、β-catenin和p120 ctn表达与PSH中立方细胞的表达情况相同.结论PSH中的立方细胞可能是增生的Ⅱ型肺泡细胞,而多角形细胞为肿瘤的实质细胞且缺乏分化成熟的上皮细胞所具有的E-cadherin/catenin复合体.血管瘤样区的腔内衬细胞是与立方细胞相同的上皮细胞而非血管内皮细胞.     

Discrimination of parakeratinised odontogenic keratocysts from other odontogenic and non-odontogenic cyst types by expression of a 38kd cell-surface glycoprotein

We have identified strong expression of a 38-kD cell surface glycoprotein (gp38), a marker of basal cell carcinomas (BCCs), in basal and suprabasal epithelial cell membranes of parakeratinised odontogenic keratocysts. In contrast, orthokeratinised cysts and most other odontogenic cyst types, ameloblastomas, normal stratified oral epithelium, cell rests of Malassez and glands of Serres, all proved negative. To our knowledge this is the first histochemical marker to distinguish between these major cyst types. It has obvious uses in the diagnosis of inflamed keratocysts and the separation of ameloblastomas from BCCs and may find a role in studies of the developmental biology of other odontogenic structures.