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981.
目的:了解反义VEGF寡脱氧核苷酸(ODN)对人胶质细胞瘤系(A172细胞)VEGF表达的抑制作用。方法:应用半定量PCR、免疫组化方法分别了解细胞内VEGF mRNA和蛋白的变化,并利用ELISA检测培养上清液中VEGF蛋白的含量。结果:A172细胞经反义VEGF ODN作用后VEGF mRNA的表达量明显减少,且随其浓度的增加而明显减少,但其表达量不受正义和错义VEGF ODN的影响。当A172细胞经50μmol·L~(-1)反义VEGF ODN作用后,细胞内及上清液内VEGF蛋白水平较对照显著减少。结论:反义VEGF ODN特异地抑制A172细胞VEGF mRNA和蛋白的表达。 相似文献
982.
An 8-year-old boy was found to have primary moyamoya disease associated with a brain stem glioma. For over 3 years the child
had experienced transient ischemic attacks induced by hyperventilation. One month before referral to our hospital he had presented
with progressive left facial nerve palsy. Magnetic resonance imaging showed a cystic mass in the lower pons. Angiography revealed
severe bilateral stenosis of the internal carotid arteries and prominent moyamoya vessels in the basal ganglia. Partial resection
of the tumor yielded a histological diagnosis of pilocytic astrocytoma. Local radiation therapy reduced the size of the tumor.
Anastomosis of the superficial temporal arteries and middle cerebral arteries on both sides was then performed. After direct
bypass surgery, the patient remained in a good condition for a 5-year follow-up period. Clinical investigation of the coincidence
of primary moyamoya disease and brain stem glioma led the authors to conclude that these two diseases coexisted independently.
Received: 25 December 1998 相似文献
983.
Shanop Shuangshoti Woranart Mitphraphan Somruetai Kanvisetsri Lisa Griffiths Yot Navalitloha Wichai Pornthanakasem Apiwat Mutirangura 《Neuropathology》2000,20(3):228-232
A 5‐year‐old girl who developed progressive headache, vomiting, and left hemiparesis was found to have a cystic tumor with an enhanced mural nodule in the right frontoparietal region on a computed tomography examination. The lesion was histologically and ultrastructurally verified as an astroblastoma, an uncommon neuroepithelial tumor of uncertain origin. Molecular analysis using 17 microsatellite markers on chromosomes 9, 10, 11, 17, 19, and 22 showed loss of heterozygosity at the D19S412 locus on the long arm of chromsome 19. This observation suggests that there is a tumor suppressor gene in this chromosomal region, which plays a role in the pathogenesis of astroblastoma. 相似文献
984.
p16 mRNA及其蛋白、EGFR在脑胶质瘤中的表达 总被引:4,自引:0,他引:4
目的:通过观察脑胶质瘤中p16 mRNA及其蛋白、EGFR的表达,探讨其在脑胶质瘤发生、发展中的作用。方法:应用原位杂交技术和免疫组织化学方法在38例人脑胶质瘤中检测p16 mRNA及其蛋白、EGFR的表达。以7例正常组织为对照。结果:p16mRNA和p16蛋白在Ⅲ~Ⅳ级、Ⅰ~Ⅱ级胶质瘤、正常组织中的阳性表达率分别为8.3%,16.7%;53.7%,57.7%;71.4%,85.7%。EGFR在Ⅲ 相似文献
985.
Machine learning in preoperative glioma MRI: Survival associations by perfusion‐based support vector machine outperforms traditional MRI
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986.
采用DextranT-40桥联法将抗人脑胶质瘤单克隆抗体SZ-39与肿瘤化疗药物氚标记阿霉素交联,制各成免疫交职物3H-Adr(阿霉素)-PAD(多醛葡聚糖)-MAb(单克隆抗体)SZ-39在荷瘤鼠体内研究免疫交联物药代动力学特征。结果显示Adr-MAbSZ-39在相关肿瘤内浓聚,发挥了单抗高选择性作用,为胶质瘤的治疗提供了高效低毒的新一代良药。但在肝内摄入较多,为其可能的副作用。血药浓度-时间曲线符合三室线性模型,T1/2长于游离Adr,有效分布体积大于游离Adr,以2周问隔多疗程治疗为佳。 相似文献
987.
Issei Tachibana Justin S. Smith Kazunari Sato Sandra M. Hosek David W. Kimmel Robert B. Jenkins 《American journal of medical genetics. Part A》2000,92(2):136-141
Epidemiological studies suggest that some familial aggregations of glioma may be due to inherited predisposition. Many genes involved in familial cancers are frequently altered in the corresponding sporadic forms. We have investigated several genes known to be altered in sporadic gliomas for their potential contribution to familial glioma. Fifteen glioma patients with a family history of brain tumors were identified through the Mayo Clinic Department of Neurology (nine diffuse astrocytomas, two oligodendrogliomas, two mixed oligoastrocytomas, one pilocytic astrocytoma, and one pineal glioma). Eleven of the propositi had one or more first degree relative with a glioma. Lymphocyte DNA was derived from each of the patients and analyzed by polymerase chain reaction (PCR) and direct sequencing of the PTEN, p53, p16INK4A/p14ARF, and CDK4 genes. In addition, fluorescence in situ hybridization (FISH) was performed on EBV‐transformed lymphocytes from each affected individual to detect germline copy number of the p16INK4A/p14ARF tumor suppressor region. A p53 germline point mutation was identified in one family with some findings of Li‐Fraumeni syndrome, and a hemizygous germline deletion of the p16INK4A/p14ARF tumor suppressor region was demonstrated by FISH in a family with history of both astrocytoma and melanoma. Thus, whereas germ‐line mutations of PTEN, p53, p16INK4A/p14ARF, and CDK4 are not common events in familial glioma, outside of familial cancer syndromes, point mutations of p53 and hemizygous deletions and other rearrangements of the p16INK4A/p14ARF tumor suppressor region may account for a subset of familial glioma cases. Collectively, these data lend genetic support to the heritable nature of some cases of glioma. Am. J. Med. Genet. 92:136–141, 2000. © 2000 Wiley‐Liss, Inc. 相似文献
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