离子对比色法测定硫酸阿托品滴眼液中主药的含量

目的:建立以离子对比色法测定硫酸阿托品滴眼液中主药含量的方法。方法:在一定pH值递质中,硫酸阿托品与酸性染料溴甲酚绿结合成稳定的离子对,用氯仿提取离子对,在420nm波长处测定其吸收度并计算其含量,将其与《中国医院制剂规范》采用的中和法结果进行比较。结果:2种方法所得结果含量均在规定范围内,无显著性差异。结论:本方法快捷、简便,适合医院制剂的快速分析。     

小剂量铁剂治疗孕妇缺铁性贫血的疗效观察

目的:观察小剂量硫酸亚铁治疗孕妇缺铁性贫血(IDA)的临床效果。方法:IDA 106例孕妇随机分为治疗组和对照组各53例,前组予以硫酸亚铁5 mg/(kg·d),后组给硫酸亚铁30 mg/(kg·d)。结果:两组治疗效果总有效率比较差异无显著性(P>0.05),达到治愈时间前组平均(46.3±9.3)d,后组(44.1±8.7)d,差异无显著性(P>0.05)。结论:口服小剂量硫酸亚铁治疗IDA疗效满意,价格低廉,副作用少,值得基层医院推广应用。     

静脉输注硫酸镁对腹腔镜胆囊切除手术应激反应的影响

目的观察硫酸镁对手术应激反应的影响。方法 40例择期在全身麻醉下行腹腔镜胆囊切除术(LC)患者随机分为硫酸镁(MgSO4)组和生理盐水(NS)组,每组20例。MgSO4组于麻醉前30 min静脉输注MgSO450 mg.kg-1.h-1至手术结束;NS组以生理盐水代替MgSO4。记录麻醉诱导前、气腹期及拔管时BP和HR的变化。采血测定血清皮质醇(Cor)、醛固酮(Ald)和血糖(BG)水平。结果与麻醉前比较,术中两组HR、BP、Cor、Ald和BG均明显升高(P<0.05),但MgSO4组低于NS组(P<0.05)。结论静脉输注硫酸镁能在一定程度上抑制腹腔镜胆囊切除术中的应激反应。     

两种不同测定方法监测人血浆中依替米星浓度的比较

目的:比较HPLC法和微生物法测定人血浆中依替米星浓度的差异。方法:选取入院时确诊为复杂尿路感染而肾功能正常患者6名,给予硫酸依替米星注射液200mg,qd,加入9.0mg/mL(0.9%)氯化钠注射液250mL中静脉滴注,连续用药7d,于第2天给药后及第5天给药前取对侧静脉采血2mL,分别采用HPLC法和微生物法测定血浆中药物的浓度。结果:HPLC法平均回收率为96.38%,日内、日间RSD分别为5.04%和6.21%;微生物法平均回收率为101.78%,日内、日间RSD分别为4.95%和6.03%。结论:两种方法回收率和精密度基本一致,受试者血浆中依替米星浓度测得值差异无统计学意义(P>0.05)。     

CHPF promotes malignancy of breast cancer cells by modifying syndecan-4 and the tumor microenvironment

Breast cancer is the leading cause of cancer-related deaths in women worldwide. Several studies have indicated that abnormal chondroitin sulfate (CS) chains accumulate in breast cancer tissues; however, the functions and dysregulation of CS synthases are largely unknown. Here, we demonstrate that chondroitin polymerising factor (CHPF) is frequently upregulated in breast cancer tissues and that its high expression is positively associated with tumor metastasis, high stages, and short survival time. CHPF modulates CS formation in breast cancer cells. Additionally, we found that CHPF promotes tumor growth and metastasis accompanied by an increase in G-CSF levels and the number of myeloid-derived suppressor cells in tumor tissue. We revealed that tumor cell-derived G-CSF is co-localised with CS on the cell surface. Interestingly, our study is the first to identify that syndecan-4 (SDC4) is modified by CHPF and that it is involved in CHPF-mediated phenotypes. Moreover, breast cancer patients with high expression of both SDC4 and CHPF had worse overall survival compared to other subsets, which implied the synergistic effects of these two genes. In summary, our results indicated that the upregulation of CHPF in breast cancer contributes to the malignant behaviour of cancer cells, thereby providing novel insights on the significance of CHPF-modified SDC4 in breast cancer pathogenesis.     

The Short Time Exposure (STE) test for predicting eye irritation potential: Intra-laboratory reproducibility and correspondence to globally harmonized system (GHS) and EU eye irritation classification for 109 chemicals

Short Time Exposure (STE) test is an easy in vitro eye irritation test that assesses cytotoxicity in SIRC cells (rabbit corneal cell line) following a 5 min dose treatment. To assess intra-laboratory reproducibility, medium control, three vehicles (saline, saline containing 5% (w/w) dimethyl sulfoxide, and mineral oil) and three standard chemicals (sodium lauryl sulfate, calcium thioglycolate, and Tween 80) were evaluated. Assessments were repeated 30 times for vehicles and 18 times for standard chemicals; resulting in almost the same cell viability and a low coefficient of variation value. In addition, the STE eye irritation rankings of three standard chemicals, as calculated on the cell viabilities in 5% and 0.05% solutions were in agreement in all tests. Based on these results, high intra-laboratory reproducibility was confirmed.In addition, the irritation category (irritant and non-irritant) was evaluated for 109 chemicals with STE test, globally harmonized system (GHS) classification, and European Union (EU) classification. The results of the evaluation found the STE classification to have an accuracy with GHS classification of 87% and with EU classification of 83%, which confirmed the excellent correspondence.The correspondence of STE rankings (1, 2, and 3) based on the prediction model by STE test with the eye irritation rankings by GHS (non-irritant, categories 2 and 1) and EU (non-irritant, R36, and R41) was 76% and 71%, respectively.Based on the above results, STE test was considered to be a promising alternative method for assessing eye irritation that has high intra-laboratory reproducibility as well as an excellent predictability of eye irritation.     

地塞米松联合硫酸镁预防长春瑞滨所致静脉炎

目的　长春瑞滨是目前最常用的化疗药物之一,它是缓和的发疱剂,能引起静脉炎。本研究旨在探讨地塞米松联合硫酸镁预防长春瑞滨所致静脉炎的效果。方法　前瞻性观察我科３００例初治晚期非小细胞肺癌患者,化疗方案包含长春瑞滨,每周期评估静脉炎的程度。全组分为试验组和对照组各１５０例,试验组使用长春瑞滨前后用地塞米松２.５ｍｇ静脉注射,５０％硫酸镁湿敷静脉穿刺点以上皮肤,从静脉用药前１０ｍｉｎ至结束后４～６ｈ;对照组仅用药前后给予地塞米松２.５ｍｇ静脉注射,未用硫酸镁湿敷。结果　试验组静脉注射长春瑞滨７９１次,其中３９次（４.９３％）发生静脉炎;对照组予８０７次静脉注射长春瑞滨,９８次（１２.１４％）发生静脉炎,两组差异有统计学意义（Ｐ＜０.０５）。试验组有８例发生静脉炎（５.３３％）,对照组１９例发生静脉炎（１２.６７％）,两组差异有统计学意义（Ｐ＜０.０５）。结论　地塞米松联合硫酸镁能预防长春瑞滨治疗非小细胞肺癌患者所致静脉炎的发生,增加患者对化疗的依从性。     

注射用硫酸头孢匹罗的制备

目的:研究并确定注射用硫酸头孢匹罗的处方及无菌粉末分装工艺。方法:采用溶析结晶法,制备出合适松密度的无菌碳酸钠结晶,对无菌粉末进行物理化学性质测定,制定合理的处方及制备工艺。结果:确定了注射用硫酸头孢匹罗处方为主药硫酸头孢匹罗1 190 g,无水碳酸钠242 g。结论:该处方设计合理,可实现工业化大生产。     

人硫酸酯酶１基因的作用机制及抗瘤活性

人硫酸酯酶１（ｈＳｕｌｆ-１）能够使细胞表面的硫酸乙酰肝素蛋白聚糖（ＨＳＰＧｓ）脱硫酸化,从而抑制下游肝素结合生长因子信号途径的激活。人恶性肿瘤中ｈＳｕｌｆ-１常处于失活状态,转染外源性ｈＳｕｌｆ １能够抑制癌细胞的增殖、迁移和侵袭,因此ｈＳｕｌｆ-１是肿瘤基因治疗的一个很有潜力的靶点。     

单甘酯对血小板粘附聚集功能的影响

观察了单甘酯（Ｇｌｙｃｏｌ ｍａｎｎａｔｅＳｕｌｆａｔｅ,ＧＭＳ）对血小板聚集,粘附功能及小鼠尾出血时间的影响。结果表明单甘酯２５,５０ｍｇ．ｋｇ＾－１剂量组对血小板粘附有显著抑制作用,Ｐ〈０．０１;２５,５０,１００ｍｇ．ｋｇ＾＆－１能明显抑制胶原和花生四烯酸诱导的血小板聚集,对ＡＤＰ诱导的血小板聚集无抑制作用。单甘酯４０,８０ｍｇ．ｋｇ＾－１可明显延长小鼠尾出血时间。结果证明单甘酯有明显的抗血