日本血吸虫成虫31/32KD抗原的纯化及其免疫化学特性

本文采用超凝胶柱层析法分离纯化日本血吸虫成虫31/32KD抗原。分离的抗原经银染色及免疫印迹法分析证明已达免疫纯及生化纯级。该抗原PAS染色呈阴性,经过碘酸钠处理后不失去活性,在琼脂糖凝胶电泳中趋向阳极。鉴于血吸虫成虫31/32KD组分是一种主要血清学抗原,它的分离纯化为改进和标化血吸虫病的诊断提供了条件。     

腰椎间盘膨出CT诊断标准的探讨

在经椎间盘中心平面的ＣＴ扫描图像上，对下腰段８２个正常椎间盘（男４９个，女３３个）及相应平面后方之硬胶囊、椎管的矢径、横径和面积进行了测量，并用指数形式来表示前、后二者间各项指标的比例关系。通过与４０个（男２７个、女１３个）膨出椎间盘的比较分析，发现硬膜囊面积／椎间盘面积、硬膜囊矢径／椎间盘矢径及椎管矢径／椎间盘矢径等三个指数在椎间盘膨出的诊断中最有价值。     

踝/肱指数对下肢动脉硬化性闭塞症缺血程度的评价

目的：利用检测下肢动脉硬化性闭塞症患的踝/肱指数对其下肢缺血程度进行评估。方法：采用以色列Flo-link 500T血管多普勒诊断仪对我院50例确诊为下肢动脉硬化性闭塞症患进行了分组踝/肱指数的检测。结果：踝/肱指数在0．5—0．9之间，常提示下肢动脉或近端有单水平阻塞；踝/肱指数在0．45以下常提示患下肢缺血程度严重，为坏疽前期静息痛表现；踝/肱指数在0．30以下多伴有缺血性溃癌或坏死。结论：踝/肱指数的检测可以反映下肢缺血程度，指导临床选择治疗方法，判断愈后及疗效，其无创性、可重复性、价格低康，更易被患接受。     

磁共振水成像的临床应用及其进展

磁共振水成像是近年来磁共振成像研究的重大进展之一。具有无创，无辐射的特点，它利用重T2加权成像使含水器官显影，应用于胰胆管，泌尿系，椎管，涎管，内耳迷路，输卵管等含水器官的显示，具有良好的应用前景。     

髋关节滑膜软骨瘤病误诊1例

患者女,33岁,农民.因右膝关节疼痛8年、右髋部腹股沟区疼痛6年、加重半年于2001年12月入院.起病以来无发热、消瘦,无嗜酒史及糖皮质激素服用史,无外伤史.在外院曾诊断为右股骨头缺血性坏死并于1999年诊断为右髋关节结核后口服抗痨药物半年,因症状无好转自行停药.入院前行走100米后即感右髋疼痛.……     

Potential diagnostic value of sampling oral mucosal surfaces for Actinobacillus actinomycetemcomitans in young adults*

Actinobacillus actinomycetemcomitans has been implicated in the pathogenesis of several forms of early onset and refractory adult periodontitis. Early diagnosis of colonization of the oral cavity might be of importance in order to initiate preventive measures. The aim of the present study was to determine the potential diagnostic value of oral mucosal and salivary tests to identify, among healthy young men with no or minor periodontal disease, individuals colonized by A. actinomycetemcomitans. Two hundred and one male recruits. 18–25 yr of age, took part in the present study. Mean values of periodontal parameters suggested only minor periodontal disease. Of the sites, 64.8±17.6% (mean ± SD) had a periodonta) probing depth (PPD) of 1 or 2 mm. only 1.6±2.9% deep sites of 5 mm were detected. More than 1000 subgingival and extracrevicular samples were selectively cultivated for A. actinomycetemcomitans. The organism was isolated in 55 subjects (21%). The odds for presence of at least 1 deep site of 5 mm was increased by a factor 1.99 if A. actinomycetemcomitans could be recovered. In identifying subjects colonized by A. actinomycetemcomitans. diagnostic test parameters sensitivity and predictive value for a negative test were 74.5±5.9% and 91.1±2.3%', respectively, for both saliva and dorsum of tongue samples. In contrast, pooled subgingival plaque from mesial surfaces of 1st molars was only 34.5±6.4% sensitive: the negative predictive value was 80.2±3.0%. The results point to a high diagnostic value of oral mucosal and especially saliva samples to identify young adult individuals colonized by A. actinomycetemcomitans.     

IGFBP-1检测在妊娠晚期气囊引产中的临床意义

【目的】比较宫颈Bishop评分和宫颈胰岛素样生长因子结合蛋白 1(IGFBP 1)检测在妊娠晚期气囊助产中的临床意义。【方法】将妊娠晚期有引产指征的 15 0例初产妇随机分成 3组 ,每组 5 0例。A组在气囊助产前使用宫颈IGFBP 1检测作为引产时机预测 ,B组在气囊助产前使用宫颈Bishop评分作为引产时机预测 ,C组则单纯催产素引产同时使用宫颈Bish op评分作为引产时机预测。【结果】总引产时间、潜伏期、活跃期、第一产程、第二产程 ,A、B两组较C组短 (P <0 0 1) ;A组总引产时间较B组短 (P <0 0 5 ) ;A组潜伏期、活跃期、第一产程、第二产程的较B组短 ,但差别无统计学意义 ;阴道分娩率A组 >B组 >C组 ,剖宫产率A组 相似文献   

化疗药物外渗后局部组织损伤处理的实验研究

目的根据化疗药物外渗后局部组织损伤的特点寻找理想的处理方法。方法制作化疗药物外渗的动物坏死模型,分别采用临床常用的三种处理方法进行动物实验,进行肉眼观察及组织学观察。结果1∶5 000呋喃西林加季德胜蛇药外敷对化疗药物外渗所致的局部组织坏死疗效最佳,其次为复方利多卡因,50%硫酸镁外敷疗效甚微,对照组无效。结论临床处理化疗药物外渗应根据其局部特点选择合理有效的方法。     

Testing for skin sensitization according to the notification procedure for new chemicals: the Magnusson and Kligman test

The notification procedure for new chemicals in the European Union (called the Chemicals Act in Germany) requires a skin sensitization test when the amount of a new chemical produced exceeds 100 kg/year. The preferred test is that of Magnusson and Kligman; more than 90% of the tests submitted are performed with it. Though the Magnusson and Kligman test is described in the literature, and in the test guidelines of the European Union and of the OECD, discrepancies do occur in the performance of the test between test laboratories. In this paper, recommendations are given for standardized performance of the Magnusson and Kligman test.