2型糖尿病大鼠血浆同型半胱氨酸与阴茎海绵体内NOS和内源性CO的相关性研究

目的:研究2型糖尿病性大鼠血浆同型半胱氨酸(Hcy)与阴茎海绵体内NOS和内源性CO的相关性。方法:选取3月龄雄性Wistar大鼠50只,随机选取10只为对照组(A组);高糖高脂饲料饲养4周后从其他40只大鼠中筛选出30只构建成功的糖尿病(DM)大鼠模型,随机分成3组:DM大鼠组(B组);胰岛素治疗组(C组)和叶酸+维生素B12治疗组(D组)。8周及12周后注射阿朴吗啡观察各组大鼠阴茎勃起情况。12周后测各组大鼠血浆总Hcy含量及阴茎海绵体内NOS活性和CO含量。结果:与A组比较,B组大鼠血浆Hcy浓度明显升高,阴茎勃起功能明显降低,阴茎海绵体NOS活性和CO含量均下降,差异有显著性(P<0.01)。2型DM大鼠中高Hcy血症发生率为55%。与B组比较,C组和D组中大鼠血浆Hcy浓度显著下降,阴茎勃起功能、阴茎海绵体NOS活性均升高(P<0.01),Hcy与NOS(rA=-0.89,rB=-0.76,rC=-0.91,rD=-0.91)及CO含量(rA=-0.82,rB=-0.77,rC=-0.93,rD=-0.81)均呈负相关。结论:2型DM大鼠血浆中的高Hcy可能是引起阴茎海绵体NOS活性下降、CO含量下降,进而导致DM ED发病的分子机制之一。胰岛素、叶酸和维生素B12可以改善DM大鼠的勃起功能,提高阴茎海绵体NOS活性和CO含量。     

Interfacial shear strength of bioactive‐coated carbon fiber reinforced polyetheretherketone after in vivo implantation

Despite the excellent osseointegration of carbon‐fiber‐reinforced polyetheretherketone (CFR/PEEK) with a surface hydroxyapatite (HA) coating, the bone‐implant interfacial shear strength of HA‐coated CFR/PEEK after osseointegration is unclear. We examined the interfacial shear strength of HA‐coated CFR/PEEK implants after in vivo implantation in a rabbit femur‐implant pull‐out test model. HA coating was performed by a newly developed method. Uncoated CFR/PEEK, HA‐coated blasted titanium alloy, and uncoated blasted titanium alloy were used as control implants. The implants were inserted into drilled femoral cortex, and pull‐out tests were conducted after 6 and 12 weeks of implantation to determine maximum interfacial shear strength. The HA‐coated CFR/PEEK (15.7 ± 4.5 MPa) and HA‐coated titanium alloy (14.1 ± 6.0 MPa) exhibited significantly larger interfacial shear strengths than the uncoated CFR/PEEK (7.7 ± 1.8 MPa) and the uncoated titanium alloy (7.8 ± 2.1 MPa) at 6 weeks. At 12 weeks, only the uncoated CFR/PEEK (8.3 ± 3.0 MPa) exhibited a significantly smaller interfacial shear strength, as compared to the HA‐coated CFR/PEEK (17.4 ± 3.6 MPa), HA‐coated titanium alloy (14.2 ± 4.8 MPa), and uncoated titanium alloy (15.0 ± 2.6 MPa). Surface analysis of the removed implants revealed detachment of the HA layer in both the HA‐coated CFR/PEEK and titanium alloy implants. The proposed novel HA coating method of CFR/PEEK significantly increased interfacial shear strength between bone and CFR/PEEK. The achieved interfacial shear strength of the HA‐coated CFR/PEEK implant is of the same level as that of grit‐blasted titanium alloy with HA. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1618–1625, 2012     

10% 5-氨基酮戊酸光动力疗法联合卡介菌多糖核酸注射液治疗面部扁平疣临床观察

目的探讨10%5-氨基酮戊酸光动力疗法(ALA-PDT)联合卡介菌多糖核酸注射液治疗面部扁平疣的临床疗效。方法将70例面部扁平疣患者按治疗方法不同随机分为观察组和对照组,各35例。观察组采用10%ALA-PDT联合卡介菌多糖核酸注射液治疗,对照组采用二氧化碳(CO_2)激光联合卡介菌多糖核酸注射液治疗。若第1次治疗皮损未完全消退,需进行第2次或第3次治疗,每次治疗间隔2周,观察第2周、第4周、第6周及治疗结束后3个月时的疗效和不良反应。结果两组患者治疗后与治疗前比较,皮损均明显消退及改善;治疗后6周,观察组总有效率为88.57%(31/35),对照组为68.57%(24/35),差异有统计学意义(P0.05)。随访3个月,观察组复发率为2.86%(1/35),对照组复发率为17.14%(6/35),差异有统计学意义(P0.05)。结论 10%ALA-PDT联合卡介菌多糖核酸注射液治疗面部扁平疣安全有效,并降低其复发率。     

Electrochemical,spectroscopic, and molecular docking studies of the interaction between the anti-retroviral drug indinavir and dsDNA

In this study, an electrochemical DNA biosensor was developed using a straightforward methodology to investigate the interaction of indinavir with calf thymus double-stranded deoxyribonucleic acid (ct-dsDNA) for the first time. The decrease in the oxidation signals of deoxyguanosine (dGuo) and deoxyadenosine (dAdo), measured by differential pulse voltammetry, upon incubation with different concentrations of indinavir can be attributed to the binding mode of indinavir to ct-dsDNA. The currents of the dGuo and dAdo peaks decreased linearly with the concentration of indinavir in the range of 1.0–10.0 μg/mL. The limit of detection and limit of quantification for indinavir were 0.29 and 0.98 μg/mL, respectively, based on the dGuo signal, and 0.23 and 0.78 μg/mL, respectively, based on the dAdo signal. To gain further insights into the interaction mechanism between indinavir and ct-dsDNA, spectroscopic measurements and molecular docking simulations were performed. The binding constant (K_b) between indinavir and ct-dsDNA was calculated to be 1.64 × 10⁸ M⁻¹, based on spectrofluorometric measurements. The obtained results can offer insights into the inhibitory activity of indinavir, which could help to broaden its applications. That is, indinavir can be used to inhibit other mechanisms and/or hallmarks of viral diseases.     

经皮二氧化碳监测在肥胖患者行腹腔镜胃旁路术中的应用

目的研究肥胖患者行腹腔镜下胃旁路术中经皮二氧化碳分压(P_(TC)CO_2)监测评估PaCO_2的准确性和相关性。方法择期行腹腔镜下胃旁路术的患者22例,男9例,女13例,年龄19~55岁,体重86~160kg,BMI35kg/m~2,ASAⅠ或Ⅱ级,在麻醉后(气腹前)和气腹后30、60和120min时测量PaCO_2、P_(ET)CO_2和P_(TC)CO_2。并计算PaCO_2-P_(ET)CO_2和PaCO_2-P_(TC)CO_2的差值,采用Bland-Altman分析、相关与回归和确切概率法分析PaCO_2与P_(ET)CO_2、PaCO_2与P_(TC)CO_2的线性回归方程和一致性界限(LOA)。结果 1例男性患者因麻醉诱导时使用了去氧肾上腺素而被排除出本研究。余患者PaCO_2-P_(ET)CO_2的差值为(10.3±2.3)mm Hg,PaCO_2-P_(TC)CO_2的差值为(0.9±1.3)mm Hg。PaCO_2与P_(ET)CO_2之间的线性回归方程为P_(ET)CO_2=11.58~+0.57×PaCO_2(r2=0.64,P0.01);PaCO_2与P_(TC)CO_2之间的线性回归方程为P_(TC)CO_2=0.60~+0.97×PaCO_2(r2=0.89,P0.01),气腹后30、60和120min时P_(ET)CO_2和P_(TC)CO_2与PaCO_2具有明显相关性(P0.01)。PaCO_2-P_(ET)CO_2差值的95%LOA为(10.3±4.6)mm Hg;PaCO_2-P_(TC)CO_2差值的95%LOA为(0.9±2.6)mm Hg。结论肥胖患者行腹腔镜下胃旁路术中,P_(TC)CO_2比P_(ET)CO_2更精确地评估PaCO_2。     

二氧化碳充气在妇科术后无痛结肠镜检中的安全性研究

目的 比较妇科手术后行结肠镜检查中注入空气与二氧化碳(carbon dioxide,CO2)的效果. 方法 选取我院妇科手术后行常规无痛结肠镜检查患者62例,采用随机数字表法分为C02组和空气组(每组31例),结肠镜检查中分别注入C02、空气.记录两组患者结肠镜检查成功率、操作时间、丙泊酚用量、恢复时间及检查后不同时间的腹痛程度,比较检查前后HR、MAP、Sp02、经皮二氧化碳分压(transcutaneous carbon dioxide partial pressure,PtcC02)的变化,VAS评估检查后15 min、1h、3h、6h及24 h腹痛程度. 结果 两组患者检查成功率、操作时间、丙泊酚用量和恢复时间差异无统计学意义(P＞0.05);两组患者检查前、检查中及检查后HR、MAP、Sp02、PtcCO2差异无统计学意义(P＞0.05);C02组患者检查后15 min、1h、3h、6h、24 h时点VAS评分均显著低于空气组[(21.6±9.5)分比(46.7±14.1)分、(14.4±5.4)分比(32.6±9.6)分、(9.1±3.6)分比(22.9±8.4)分、(4.3±1.1)分比(9.7±3.5)分、(2.3±1.1)分比(4.1±1.9)分](P＜0.05). 结论 妇科术后患者行无痛结肠镜检查时注入CO2代替空气安全有效,且能减轻检查后腹痛程度.     

疼痛护理干预对二氧化碳激光治疗皮肤病患者焦虑状况的影响

目的 探讨疼痛护理干预对二氧化碳激光治疗皮肤病患者焦虑状况的影响。方法 选取2022年 1月-10月于我院皮肤科门诊采用二氧化碳激光治疗皮肤病的500例患者为研究对象,采用随机数字表法 分为对照组和观察组,每组250例。对照组采用常规护理干预,对照组在对照组基础上实施疼痛护理 干预,比较两组负性情绪、疼痛程度及护理满意度。结果 观察组护理满意度高于对照组（P＜0.05）; 观察组护理后HAMA、HAMD评分均低于对照组（P＜0.05）;观察组术后1、2 h NRS评分低于对照 组（P＜0.05）。结论 对二氧化碳激光治疗皮肤病的患者实施疼痛护理干预可有效缓解其焦虑程度,同时 提升就医体验感,提高满意度,值得临床应用。     

二氧化碳激光在皮肤美容中的应用效果

目的 分析二氧化碳激光在皮肤美容中的应用效果。方法 选取2021年6月-2022年6月我院皮肤 美容科收治的70例患者为研究对象,采用随机数字表法分为观察组与对照组,每组35例。对照组给予常规 治疗方式,观察组给予二氧化碳激光治疗,比较两组临床疗效、皮损情况、皮肤状况及疼痛程度。结果 观察组治疗总有效率为100.00%,高于对照组的88.57%,差异有统计学意义（P＜0.05）;观察组治疗后色 素痣、疣状痣、皮脂腺痣、乳头状瘤数量均小于对照组,差异有统计学意义（P＜0.05）;观察组治疗后皮 肤色泽、质地评分高于对照组,VAS评分低于对照组,差异有统计学意义（P＜0.05）。结论 二氧化碳激 光在皮肤美容中的应用效果确切,可有效改善患者的皮肤色泽及质地,促进皮损恢复,还能减轻疼痛,具 有一定的临床应用价值。