Fumonisins as a possible contributory risk factor for primary liver cancer: A 3-year study of corn harvested in Haimen, China, by HPLC and ELISA

Employing HPLC fluorometry, gas-liquid chromatography (GLC) and a novel enzymelinked immunosorbent assay (ELISA) based on a monoclonal antibody, 40 corn samples, each collected in 1993 from agricultural stocks for human consumption in Haimen (Jiangsu County) and Penlai (Shandong Province), high- and low-risk areas for primary liver cancer (PLC) in China, respectively, were analysed for fumonisins (FBs), aflatoxins (AFs) and trichothecenes. Levels and positive rates of FBs and deoxynivalenol (DON) were significantly higher in Haimen than in Penlai. ELISA of the 40 corn samples harvested in the two areas in 1994 revealed that FB contamination levels and rates in these areas were comparable to those observed in 1993 in Haimen. ELISA analysis of 1993 and 1994 products revealed a wide occurrence of AFB₁ but the positive rates as well as levels were not significantly different between these areas. ELISA of the same sample number of corn harvested in 1995 revealed that FB contamination in Haimen was significantly higher than in Penlai. These 3-yearly surveys of corn samples (240 in total) demonstrated that corn harvested in Haimen was highly contaminated with FBs and that the contamination level, as well as positive rate in 1993 and 1995, were 10–50-fold higher than those in Penlai, suggesting FBs as a risk factor for promotion of PLC in endemic areas, along with the trichothecene DON. Co-contamination with AFs, potent hepatocarcinogens, was assumed to play an important role in the initiation of hepatocarcinogenesis.     

多种底物在免疫金银染色检测抗核抗体试验中的联合应用

将多种底物联合应用于免疫金银染色检测ANA试验,与各底物单独应用比较,可明显改善检测的敏感性与核型显示。其中,两种鼠脏器印片联合应用的结果可与Hep-2细胞的结果相当,三种及四种鼠脏器印片联合应用的结果可与Hep-2细胞和一种鼠脏器印片联合应用的结果相当,且均不使正常人ANA阳性率明显升高。     

提高抗恶性疟独特型单抗克隆出现频率的方法学探讨

用Protein A-Sepharose亲和层析法提纯McAb-IgG,皮下多点和腹腔免疫雄性Wistar大鼠,间隔两周共3次,融合前3天采用腹腔、尾静脉和脾内注射等三种加强免疫方法,以间接ELISA法和抗体竞争ELISA法筛选Anti-Id阳性克隆。结果显示:融合前大鼠血清抗小鼠IgG或抗免疫原McAb-IgG滴度,静脉组和脾内组比腹腔组高一个滴度;Anti-Id阳性克隆出现频率,腹腔组为0.9％和0.3％,静脉组为11.0％和13.3％,脾内组为16.4％和12.9％,静脉组和脾内组明显高于腹腔组(P<0.01);上述三种加强免疫方法对抗小鼠IgG阳性克隆出现频率无明显影响(P>0.05);与腹腔加强免疫组相比,静脉和脾内加强免疫组也可提高细胞融合效果(P<0.01).本实验结果提示,腹腔和皮下多点多次长程基础免疫结合静脉或脾内加强免疫是制备Anti-Id McAb比较好的免疫方案。     

脾脏切除对胸腺和淋巴结免疫功能的影响

报道脾脏切除（脾切）对动物胸腺和淋巴结免疫功能的影响，脾切后３ｗ大鼠胸腺细胞自发增殖及ＣｏｎＡ诱导增殖均无明显变化，ＣｏｎＡ诱导淋巴结细胞增殖脾切组明显高于假手术对照组，脾切后３ｗ再用ＳＲＢＣ免疫，５ｄ后小鼠腹腔淋巴结数量脾切明显高于对照组，用ＱＨＳ法测定淋巴结细胞对ＳＲＢＣ初次抗体产生脾切组则明显低于对照组。     

双特异抗体介导的人单核—巨噬细胞对荷人肝癌裸鼠的抑制作用

观察双特异性单克隆抗体介导的人单核－巨噬细胞在裸鼠体内对肝癌生长的抑制作用。用化学交联法制备双特异性单克隆抗体ＨＡｂ１８－ＭＡｂ７及ＨＡｂ１８Ｆ（ａｂ）２－ＭＡｂ７Ｆ（ａｂ＇＿２，并将其与单核－巨噬细胞－同注入荷人肝癌裸鼠体内，观察肿瘤体积的变化。     

EPF单克隆抗体杂交瘤细胞株的建立

本文用纯化的早孕因子免疫ＢＡＬＢ／Ｃ小鼠，通过鼠－鼠杂交瘤技术，成功地建立了２株分泌ＥＰＦ单克隆抗体的杂交瘤细胞。经检测，它们所分泌的抗体亚类均为ＩｇＧ１，毁交瘤染色体数目１００－１０６条，ＥＩＳＡ检测诱生腹水抗体效价为１：１．２８＊１０＾５，连续培养生物学性状稳定。抗ＥＰＦ抗原的单克隆抗体的建立，为深入研究ＥＰＦ的生物学性质、特征提供了有力的工具。     

Improved Cellular Delivery of Antisense Oligonucleotides Using Transferrin Receptor Antibody-Oligonucleotide Conjugates

This work is dedicated to the memory of the late Dr Ian Walker.     

A群脑膜炎多糖菌苗接种前后流脑流行规律的改变及今后预防对策

本文研究了在使用菌苗预防前后的流行性脑脊髓膜炎流行强度的变迁，年龄、地区及季节分布特点，人群的易感性及带菌率之间的关系，并讨论了今后的预防措施。     

抗白色念珠菌鸡卵黄抗体(IgY)的研制

目的：观察鸡蛋黄中抗体的产量、纯度、来源及稳定性。方法：应用白色念珠菌作为抗原免疫25周龄Leghorn鸡，通过改良水溶法（WD）提取蛋黄中抗体IgY，双紫外光测定抗体含量，SDS-APGE电泳检测抗体纯度，Western blot免疫印迹法测定该抗体来源，ELISA检测热处理后的抗体活性。结果：抗体含量13mg/ml蛋黄液，抗体纯度达到95%，Western blot免疫印迹证明该抗体与鸡血清中的IgG具有相同的分子量和抗原性。ELISA检测热处理后的抗体活性，其具有良好的热稳定性。结论：鸡蛋黄内含有丰富的抗体，通过WD水溶提取法可得到高产量，高纯度的特异性抗体IgY，证明其来源于鸡血清中的IgG，对热具有高度稳定性。     

抗RSV—iRNA对细胞融合病变影响的研究

用RSV-iRNA对BALB/C鼠致敏注射,以融合细胞计数法检测致敏鼠血清的抗融合活性。试验结果表明:RSV-iRNA致敏鼠血清能抑制由RSV引起的典型细胞融合病变,且这种活性可因吸附血清特异抗体而消失。