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61.
Hidehito Sakaguchi Tetsuji Kawata Shigeki Taniguchi 《The Japanese Journal of Thoracic and Cardiovascular Surgery》2004,52(2):71-74
The surgical approach to ischemic mitral regurgitation with concomitant inferior left ventricular aneurysm remains uncertain
in terms of the indication for operation and the short-and long-term outcomes. We performed concomitant mitral valve repair,
left ventricular reconstruction, and aortic valve replacement on a 71-year-old male with severe ischemic mitral regurgitation,
inferior left ventricular aneurysm, and degenerative aortic regurgitation. Postoperative status was in New York Heart Association
functional class I without mitral regurgitation 8 months after operation. We discuss, and review the procedures reported in
the literature. 相似文献
62.
Repair of large midline incisional hernias with polypropylene mesh: Comparison of three operative techniques 总被引:9,自引:0,他引:9
de Vries Reilingh TS van Geldere D Langenhorst BLAM de Jong D van der Wilt GJ van Goor H Bleichrodt RP 《Hernia》2004,8(1):56-59
Polypropylene mesh is widely used for the reconstruction of incisional hernias that cannot be closed primarily. Several techniques have been advocated to implant the mesh. The objective of this study was to evaluate, retrospectively, early and late results of three different techniques, onlay, inlay, and underlay. The records of 53 consecutive patients with a large midline incisional hernia — 25 women and 28 men, mean age 60.4 (range 28–94) — were reviewed. Polypropylene mesh was implanted using the onlay technique in 13 patients, inlay in 23 patients, and underlay in 17 patients. Either the greater omentum or a polyglactin mesh was interponated between the mesh and the viscera. The records of these 53 patients were reviewed with respect to: size and cause of the hernia, pre- and postoperative mortality and morbidity, with special attention to wound complications. Patients were invited to attend the outpatient clinic at least 12 months after implantation of the mesh for physical examination of the abdominal wall. Postoperative complications occurred in 14 (26.4%) patients. The onlay technique had significantly more complications, as compared to both other techniques. Reherniation occurred in 15 (28.3%) patients. The reherniation rate of the inlay technique was significantly higher than after the underlay technique (44% vs 12%, P=0.03) and tended to be higher than the onlay technique (44% vs 23%, P=0.22). Repair of large midline incisional hernias with the use of a polypropylene mesh carries a high risk of complications and has a high reherniation rate. The underlay technique seems to be the better technique. 相似文献
63.
64.
处女膜修补手术及麻醉方法的改进 总被引:9,自引:7,他引:2
目的;增大处女膜粘膜瓣的接触面,减少因麻醉造成的处女膜粘膜水肿,提高修复手术的成功率。方法:手术全部采用1%的卡因行粘膜表面麻醉。53例采用瓦合粘膜瓣法,3例用瓦合粘膜瓣联合阴道粘膜瓣修复法。结果:56例术后一月随访,53例处女膜孔径为一指,成功率94.64%。结论:采用1%的卡因粘膜表面麻醉,瓦合粘膜瓣法及瓦合粘膜联合阴道粘膜瓣修复法对处女膜修复是行之有效的。 相似文献
65.
由于心肌细胞的增殖能力很低,细胞移植作为一种新的治疗方法用于改善心功能及心肌活力已受到广泛的关注。目前已有胚胎干细胞、骨髓间充质干细胞和内皮干细胞在体外诱导分化为心肌细胞;动物实验中用于心肌移植的细胞有胚胎心肌细胞、造血干细胞、骨髓间充质干细胞、骨骼肌成肌细胞、内皮干细胞、肝干细胞和神经干细胞。其中成肌细胞移植用来改善心肌梗死后的心脏功能,已有临床报道,并取得成功。 相似文献
66.
不同种属脱细胞真皮与自体皮复合移植的比较性研究 总被引:5,自引:2,他引:3
目的 观察不同种属脱细胞真皮基质 (acellulardermalmatrix,ADM)与自体皮复合移植的效果 ,为异种ADM的临床应用提供理论依据。 方法 本地产白色小猪 6头 ,分为异种 (人 )ADM +自体刃厚皮组 (A组 )、同种异体 (猪 )ADM +自体刃厚皮组 (B组 )、单纯自体刃厚皮组 (C组 )及单纯自体中厚皮组 (D组 )。观察术后 2、4、8、12、2 4周内移植物存活率 ,以及移植皮片收缩程度、移植区组织学变化等情况。 结果 A、B组移植后皮片外观光滑、有弹性 ;两组均获得了满意的皮片成活率 ,并可迅速诱导成纤维细胞、血管内皮细胞等宿主修复细胞的长入 ;两组移植皮片收缩面积有大于C、D组的趋势 (P <0.0 5);术后 2 4周移植区组织与单纯中厚皮移植组织结构一致。 结论 在观察期内(复合移植后 2 4周 ) ,与自体皮复合移植时 ,同、异种ADM具有相近的生物学作用 ,异种来源的ADM可能具有更广阔的应用前景 相似文献
67.
Benyam Asefa Paul Kauler Denis Cournoyer Shirley Lehnert T. Y.-K. Chow 《Current genetics》1998,34(5):360-367
The deoxyribonucleases (DNases) have been shown genetically to be important in the vital processes of DNA repair and recombination.
The NUD1 gene, which codes for an endo-exonuclease of Saccharomyces cerevisiae, was analyzed for its role in the DNA double-strand break (DSB) repair processes. While the nud1 strain is only slightly sensitive to ionizing radiation, expression of the HO-endonuclease to introduce a DSB at the MAT locus in that strain results in cell death. Cell survival is inversely proportional to the duration of HO-endonuclease expression.
Analysis of the surviving colonies from the nud1 strain indicated that many of the survivors are sterile and that the proportion of these sterile survivors increases with
the time of HO-endonuclease expression. On the other hand, the surviving colonies from the isogenic NUD1 strain are mating-proficient. Interestingly, double mutants of nud1 rad52 are more resistant to ionizing irradiation than the rad52 strain and have a cell-survival fraction of 32% for rad52-1 nud1 and 9% for rad52::URA3 nud1 following prolonged HO-endonuclease expression, indicating that nud1 has a suppressor effect on the DSB-induced lethality in rad52. Polymerase chain reaction analysis showed that many of the nud1 survivors contained small alterations within the MAT locus, suggesting that the survivors arose through the process of non-homologous end-joining. These results suggest that
the endo-exonuclease acts at a DSB to promote DNA repair via the homologous recombination pathway.
Received: 20 July / 20 September 1998 相似文献
68.
神经肽P物质对肉芽组织成纤维细胞增殖及表皮生长因子基因表达的作用 总被引:11,自引:1,他引:10
目的:探讨感觉神经肽P物质(SP)对离体培养的肉芽组织成纤维细胞的促增殖作用及其对表皮生长因子(EGF)基因表达的调控作用。方法:采用MTT法测定SP对肉芽组织成纤维细胞的促增殖作用;采用逆转录-聚合酶链反应(RT-PCR)方法检测SP对成纤维细胞EGF基因表达的调控作用,观察时间及剂量-效应关系。结果:10^-9~10^-5mol/L的SP在体外对肉芽组织成纤维细胞均具有明显的促增殖作用(P<0.01),且具有明显的剂量依赖性(γ=0.594,P<0.01),EGF抗体只能部分抑制这一作用(与对照组比较,P<0.01;与10^-7mol/L SP组比较,P<0.05)。10^-7mol/L SP可诱导成纤维细胞EGF mRNA的表达,在作用后6h与对照组比较,差异有非常显著性意义(P<0.01);SP在10^-8~10^-6mol/L范围内可以显著促进成纤维细胞EGF mRNA表达,在10^-7mol/L达到峰值,当浓度>10^-7mol/L时,其促EGF mRNA表达的效应强度随浓度升高而有所降低,至10^-5mol/L时与对照组比较,差异无显著性意义。结论:SP对肉芽组织成纤维细胞具有明显的促增殖作用,这种作用与其诱导成纤维细胞EGF表达有关。 相似文献
69.
The syndactyly repairs of 11 hands in seven patients (average age 20.4) who had simple complete syndactyly between the third and fourth fingers were done by the use of tissue expanders in order to obtain adequate skin closure. At the first stage, a wedge type, 7 cc tissue expander was placed beneath the dorsal skin of the syndactylous digits via a vertical dorsal hand incision under local anesthesia. Inflation was begun on the 15th postoperative day. After the inflation period (average 32 days), syndactyly repair similar to the Littler technique was performed using axillary block anesthesia. During the expansion period one tissue expander became exposed and this hand was repaired by the classical skin grafting technique. In the remaining ten hands the expanded dorsal skin ensured skin closure of the digits and web reconstruction without any need for skin grafting. No complications were encountered during the postoperative period. Active and passive abduction angles, tip-to-tip length, active range of motion of the digits, and two point discrimination tests were done at follow-up (average six months). The web appearances were normal in all patients. Active abduction angles and active range of motion of the digits was always normal at the third postoperative month. Two point discrimination was 8.7 mm on the interdigital sides of the digits and there was no tip-to-tip length differences of the digits. No contractures were seen.Presented at the International Congress of Surgery of the Hand and Upper Extremity, Izmir, Turkey, September 25, 1994. 相似文献
70.
报道7例自发性食管穿孔治疗经验,并对诊断和治疗和治疗新方法进行了讨论,自发性食管穿孔早期诊断的重要依据是迅速获得完整,详细的发病史。可疑时通过食道造影,胸腔穿刺刺液的分析而确诊。一经确诊应急取早期食管修补,对发病时间长,全身情况差的病人,经积极准备后仍应急取食管修补,对不宜手术者给予食管冲洗促进穿孔愈合。同时给予有效的食管腔内外引流。 相似文献