妇科疾病中人乳头瘤病毒感染的分子流行病学调查

目的:探讨各种妇科疾病与人乳头瘤病毒感染的关系,建立不同妇科疾病和不同年龄段妇女人乳头瘤病毒感染的流行病学资料库。方法:应用反向点杂交技术分别对疾病组1 512例病人标本和正常组500例标本进行HPV检测,所得数据用SPSS 13.0软件包进行统计分析。结果:正常组500例标本中,阳性标本101例,感染率为20.2%,疾病组1 512例标本中,阳性标本523例,感染率为34.6%,其中双重感染82例,占阳性标本的15.7%,多重感染34例,占阳性标本的6.5%,而高危感染340例,感染率为22.5%,占阳性标本的65.0%,其中单纯HPV16亚型感染155例,占所有阳性标本的29.6%;不同年龄段、不同性生活史、不同妇科疾病以及不同首次性生活年龄的HPV感染检出率都有显著性差异。结论:正常组和疾病组的HPV感染率有显著性差异(2χ=36.37,P<0.01)。在HPV感染的普查中可以有重点的选择高危人群,这样更加有针对性,从而大大降低普查成本。     

东南亚及西太平洋区的丝虫病

淋巴丝虫病为东南亚、西太平洋及南太平洋的重要公共卫生问题。此为慢性病，丝虫之成虫寄生于淋巴管或淋巴结，长达10－18年之久，致淋巴循环受阻塞而形成象皮病。根据微丝蚴在人体末梢血液之出现时间，分为日间和夜间之周期型与亚周期型。媒介多达40余种。防治方法主要赖药物治疗，世界卫生组织预期以现有的药物可于2020年消灭全球之淋巴丝虫病。本将班氏丝虫、马未丝虫及旁汶丝虫发现之经过，微丝蚴定期性，病媒蚊种及防治原则，作了相当完整的综述。笔曾在中所提及的多数国家实地防治丝虫病。     

High affinity neurotrophin receptors in the human pre-term newborn, infant, and adult cerebellum

The immunohistochemical occurrence of the high affinity neurotrophin (NT) receptors trkA, trkB, and trkC is shown in the pre-term newborn, infant, and adult human post-mortem cerebellum. Immunoreactive neuronal perikarya and processes were observed in all specimens examined, where they appeared unevenly distributed in the cerebellar cortical layers and deep nuclei, and showed regional differences among cerebellar lobules and folia. The trk receptor-antibodies, tested by Western blot on human cerebellum homogenates, revealed multiple immunoreactive bands for trkA and single bands for trkB and trkC. The results obtained show the tissue localization of the trk receptor-like immunoreactivity in the human cerebellum from prenatal to adult age. The analysis for codistribution of the receptors with the relevant ligand and among the receptors in discrete cortical and deep nuclei tissue fields shows a wide variety of conditions, from a good similarity in terms of type and density of labeled structures, to a lack of correspondence, and suggests the possibility of colocalization of trk receptors with the relevant neurotrophin and among them in the cerebellar cortex. These results sustain the concept that the neurotrophin trophic system participates in the development, differentiation, and maintenance of the human cerebellar connectivity and support the possibility of a multifactorial trophic support for the neurotrophins through target-derived and local mechanisms.     

Nucleic acid spot hybridization with nonradioactive labeled probes in screening for human papillomavirus DNA sequences

We examined 161 human tissue samples using the spot hybridization technique with nonradioactive labeled DNA probes of human papillomavirus (HPV). Whole cells were spotted on nitrocellulose filters; DNA of the cells was denatured and fixed to the filter. Then the DNA spots were hybridized to nonradioactive labeled DNA and monitored by a sandwich immunoenzymatic reaction. This technique is simple, sensitive, specific, requires no special equipment, and can be used in clinical settings. HPV DNA was found in 92% of samples in which, on the basis of histologic and colposcopic criteria, its presence was suspected, as well as in 31 samples where it was not suspected.     

中西医结合治疗慢性肾炎疗效观察及其与血液流变学和微循环的相关意义分析

本文对比观察了中西医结合治疗与单纯西药治疗慢性肾炎的临床疗效,结果表明,前者缓解率及总有效率分别为79.8%和92.1%,显著高于对照组(均P<0.01)。治疗组各临床类型及各中医证型的疗效亦均明显高于对照组相对应的各型肾炎(P<0.05～0.01)。治疗组各型肾炎之血液流变学及甲皱微循环改善均较对照组为显著。两组中完全缓解加基本缓解者,该两类检查指标改善极为显著(P<0.05～0.01),而好转及无效者则无明显改善(P>0.05)。提示临床疗效与之密切相关。     

人乳头瘤病毒与呼吸道肿瘤关系的研究：Ⅰ.HPV与喉癌的关系

应用α－３２Ｐ－ｄＣＴＰ标记ＨＰＶ－１１及ＨＰＶ－１６ＤＮＡ为探针，以Ｓｌｏｔｂｌｏｔ及Ｓｏｕｔｈｅｒｎｂｌｏｔ两种核酸杂交技术，检测了取自青岛及北京两个地区的３７例喉鳞癌组织ＤＮＡ中ＨＰＶ－１１及ＨＰＶ－１６ＤＮＡ相关序列。结果表明，Ｓｌｏｔｂｌｏｔ杂交中，与ＨＰＶ－１１探针杂交阳性者占８６％（３２／３７），与ＨＰＶ－１６探针杂交阳性者占８１％（３０／３７）。在同一癌组织中，与两型探针同时呈现阳性者占８１％（３０／３７）。Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交中，与所用探针ＤＮＡ的内切酶ＰｓｔⅠ酶切图谱比较，有４６％（１３／２８）与ＨＰＶ－１６ＤＮＡ的酶切图谱相同，可定为ＨＰＶ－１６型。当用ＨＰＶ－１１探针时无一例出现与ＨＰＶ－１１相同的图谱。提示喉癌的发生与ＨＰＶ－１６有关。     

中西药物保留灌肠合并针灸治疗溃疡性结肠炎的临床研究

目的 观察中西药物保留灌肠合并针灸治疗溃疡性结肠炎(UC)的疗效.方法 对确诊的63例UC患者随机分为两组,治疗组(32例)用中西药物150 mL睡前保留灌肠合并针灸治疗,每日1次,疗程4周;对照组(31例)用强的松龙100 mg、庆大霉素16万u加生理盐水150 mL睡前保留灌肠,每日1次,疗程4周.然后比较疗效.结果 治疗组疗效明显高于对照组,总有效率分别为96.9%和77.4%,1年复发率分别为9.37%和25.8% (P<0.05).两组差异有统计学意义.结论 中西药物保留灌肠合并针灸治疗UC疗效显著,复发率低,有临床应用价值.     

PMN对链球菌组蛋白样蛋白释放的影响

为观察人多形核白细胞 (PMN)对链球菌组蛋白样蛋白 (HlpA)释放的影响 ,将PMN与链球菌以一定比例共同作用后取上清液做免疫印迹试验。发现与抗HlpA抗体特异性结合的蛋白条带。提示PMN作用于链球菌后 ,HlpA能自菌体内释放出来     

Correlation between the induction of an immediate early gene, zif/268, and long-term potentiation in the dentate gyrus.

Expression of the immediate early gene zif/268 (also termed NGFI-A, Krox 24, TIS8 and Egr-1) was investigated in awake rats following various long-term potentiation (LTP) induction protocols.zif/268 mRNA (Northern blots) and protein (immunohistochemistry) levels sharply increased following LTP, and followed a time course characteristic of other immediate early genes. When measured across 3 tetanization protocols known to produce differing degrees of LTP persistence,zif/268 induction was found to be more highly correlated with LTP duration than with the magnitude of initial LTP. These data support the hypothesis that the immediate early gene zif/268 plays a role as a third messenger in the cascade of cellular and nuclear events that govern the persistence of LTP.     

Strategic planning in academic paediatric hospitals: The need for child health input

Over the past two decades, a number of Canadian paediatric academic programs, previously operated as separate hospitals, have been integrated into larger teaching hospitals or regional health authorities. The present article describes the recent experience of the Children’s Hospital of Western Ontario within the London Health Sciences Centre (London, Ontario) to illustrate the potential deleterious effects of planning, system and program changes in a large academic hospital without child health input at the executive decision-making level. The vision of the London Health Sciences Centre Executive Leadership Team and Board of Directors was divergent from that of the paediatric health care providers, which resulted in the resignation of a number of paediatric subspecialists and compromised the ability of the Department of Paediatrics to deliver paediatric care and educate future professionals. The present article highlights the need for the involvement of paediatric stakeholders in strategic planning in the hope that other academic centres can learn from this experience.