直线偏振光近红外线照射对原代培养的兔软骨细胞增殖的影响

目的 观察不同强度直线偏振光近红外线(超激光)照射诱导软骨细胞增殖效果,探讨超激光照射对兔软骨细胞增殖的影响.方法 采用输出功率为0％(0 mW)、20％(360 mW)、40％(720 mW)、60％( 1080 mW)、80％( 1440 mW)和100％( 1800 mW)的超激光对原代培养的兔关节软骨细胞进行5 min/d连续5d的照射,然后采用CCK-8法检测细胞活力.结果 超激光强度60％组D450值为1.88±0.11,80％组D450值为1.99±0.05,分别与对照组D450值0.95±0.38比较均有显著性差异(P＜0.05);与对照组D450值比较,超激光强度20％、40％及100％组D450值虽有增高但无显著性差异(P＞0.05).与对照组相比,超激光照射能诱导软骨细胞活力增加,20％ ～80％四组细胞活力增加的比率分别为(48.75±15.4)％、(67.02±29.61)％、(97.93±11.57)％和(108.52±5.81)％,但随着照射强度增加至100％时,细胞活力增加的比率下降,比率为(62.84±31.12)％.结论 直线偏振光近红外线照射对软骨细胞有促增殖作用,直线偏振光近红外线照射对细胞的增殖作用具有强度依赖性,照射强度为80％(1440mW)软骨细胞活力增加效果最好.     

留置超滑乳胶气囊导尿管并发症的原因分析及对策

目的探讨留置超滑乳胶气囊导尿管并发症的原因及对策。方法对586例留置超滑乳胶气囊导尿管患者进行观察,回顾分析。结果留置超滑导尿管并发症与导尿方法、规范操作、留置导尿管的时间及相关知识宣教有关。结论严格无菌操作,选择合适的导尿管、缩短留置尿管的时间是降低并发症的重要措施。     

便携式多通道大容量生理信号记录仪的研制

本文介绍了采用快闪随机存储器的高精度、大容量生理信号记录仪的设计方法与电路结构,并对其与计算机进行数据通讯的方法进行了阐述。     

La super hypertension artérielle en milieu cardiologique au Burkina Faso

Super hypertension is defined as systolic BP ≥ 250 mmHg and/or diastolic BP ≥ 150 mmHg in presence or not of complications. The aim of our study was to describe the epidemiological and evolutive patterns of super hypertension in the cardiology department of the Yalgado Ouedraogo University Hospital. It was an observation cohort over a period of 26 months (July 2011 to August 2013). We recruited 34 patients, corresponding to a prevalence of 12.9% of all hypertensive patients. The median time of follow-up was 7.1 months. The mean age was 47 years old, with a sex-ratio of 1.3. Twenty-one (62%) of the patients were known hypertensive, out of who 24% were regularly rewiewed, 57% on treatment, but none was on regular medications. Dyspnoea was the reason for consulting in 38% of the cases. We noticed a fundoscopy stage III or IV in 55.9% of the cases. All patients had left ventricular hypertrophy on ECG, and 90% on echocardiography. Complication was noticed on admission in 91% of the cases. Chronic renal failure occured in 14.7% of the cases during follow-up, and overall mortality rate was 0.5 person years. Probability of survival over one month was 70% for all patients. The mean age of deceased was 35%. Presence of renal failure, unknown hypertension, and age < 45 were factors linked to death (P < 0.05). Super hypertension is a pathology of the youth, with high morbi-mortality. The prevention is done through early detection and efficient management of hypertension.     

超选择性子宫动脉栓塞术在剖宫产后大出血中的应用

目的 探讨超选择性子宫动脉栓塞术在行剖宫产术后发生大出血患者中的临床疗效.方法 选择2007年7月～2010年3月行剖宫产术后发生子宫大出血的24例患者,经产科常规方法不能有效控制子宫出血,采用血管内介入技术栓塞子宫动脉止血.结果 22例患者经超选择性插管栓塞子宫动脉后均能有效止血,同时保留了子宫及生理功能,术中术后均未发生并发症.2例患者经血管内介入栓塞后3h内再次发生大出血,1例经再次超选择血管内栓塞后成功止血,1例直接切除子宫.结论 超选择性子宫动脉插管栓塞术是治疗剖宫产术后大出血的有效治疗方法,其具有创伤小、疗效好、止血迅速可靠的特点,能够在挽救产妇生命的同时保留子宫及其生理生育功能,其安全性和疗效均较高,若血管内介入术后不能有效止血,可再次行血管内介入栓塞或者切除子宫.     

Super Bond C＆B应用于后牙金属烤瓷修复体大面积崩瓷的临床疗效研究

目的探讨Super Bond C＆B应用于后牙金属烤瓷修复体（PFM）大面积崩瓷的临床疗效。方法对2005年9月-2010年9月山西医科大学附属口腔医院修复科收治的28例PFM后牙颌面崩瓷病例采取折断面固位预备,硅橡胶取模制作金瓷覆盖冠后应用Super Bond C＆B粘结,打磨抛光完成修复。结果修复后随访观察6个月-5年,28例中25例修复后金瓷覆盖冠无松动,脱落,变色,效果良好,成功率89.29%。3例松动后重新修复,效果满意。结论 Super Bond C＆B应用于后牙PFM大面积崩瓷的修复具有美观、简便、疗效持久的优点,值得临床推广应用。     

An automatic device for determining threshold variations in antidromically activated neurons

A device was designed and constructed with the purpose of evaluating threshold variations for antidromic invasion of extracellularly recorded neurons. Identification of a neuron is carried out by two procedures, an amplitude discriminator, which isolates the spike from the baseline noise, and by a latency window which is set accordingly to the neuron's antidromic latency. During threshold evaluation, the duration of an electric pulse applied to the neuron's axon is automatically varied depending on the presence or not of an action potential. For a given spike, the stimulus is progressively decreased (-delta i) up to a point where the neuron ceases to respond and thereafter, the stimulus amplitude is progressively increased (+delta i) until slightly suprathreshold values are obtained. The procedure guarantees a discharge probability of the neuron equivalent to 50% of all applied stimuli, and the simple monitoring of the stimulus amplitude is enough to obtain the threshold value for a predetermined intensity. The reliability of this device was checked in studies related to threshold variations in neurons antidromically driven in prefrontal cortex following stimulation of the ipsi and contralateral olfactory bulb. Variations in excitability were found during and following tetanic stimulation and throughout the axon's supernormal conduction period. This technique allows the assessment of threshold variations in antidromic driving, not only in the present experimental design, but also in other conditions induced by changes in extracellular ionic concentrations, drug applications or in those produced by excitatory or inhibitory synaptic activity on the neuron under study.     

中药注射液血必净联合激素防治鼠百草枯中毒后的肺损伤

目的 探讨中药注射液血必净联合激素对大鼠百草枯中毒急性慢性肺损伤的防治作用及可能机制.方法 雄性Wistar大鼠120只平均随机分六组,每组20只:正常组(A),中毒组(B),治疗组(C,D,E,F).除A组予等量生理盐水外,其余各组予20%百草枯(PQ)100 mg/kg腹腔汴射(ip)染毒2 h后,C,D,E,F组分别予血必净(1.25 g/kg)、血必净(2.5 g/kg)、地塞米松(25 mg/kg)、血必净(2.5g/kg)+地塞米松(25 me/kg),ip,A,B组予等量生理盐水治疗,1次/d,至处死,而28 d处死者治疗7 d.观察各组大鼠反应,丁染毒后2,3,4 d每组每大各处死5只,检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)及3 d动脉血气、肺系数;于28 d处死剩余鼠,测定肺匀浆羟脯氨酸(HYP)、血清TCF-β1.A,B,F组行3 d,28 d肺病理检查.结果 治疗组巾毒症状较B组轻,F绀最轻;各治疗组不同时间点SOD,MDA及血气,TGF-β1,HYP较B组佳,治疗组巾F组最佳,其中B,F组的3 d时的SOD,MDA及28 d时的HYP,TGF-β1分别为(37.47±13.00)nmol/mL,(91.86±21.35)nmol/mL;(11.34±3.07)nmol/mL,(5.63±1.58)nmol/mL;(2.54±0.63,1.32±0.07)mg/g;(484.13±63.79,202.22±49.83)pg/mL,差异均具有统计学意义(P＜0.05).肺病理显示F组急性期肺出血、肺实变及慢性期肺纤维均较B组轻.结论 血必净联合激素于中毒早期有较强清除氧自由基、抑制脂质过氧化反应的能力,可以减轻急性肺出血、肺水肿;晚期通过抑制TGF-β1分泌和HYP的合成,减轻慢性肺纤维化.     

Axonal projections and conduction properties of olfactory peduncle neurons in the armadillo (Chaetophractus vellerosus)

Summary Extracellular unit recording was employed to study the axonal properties and efferent projections of antidromically identified neurons in the olfactory peduncle (OP) region of a primitive eutherian macrosmatic mammal, the south american armadillo (Chaetophractus vellerosus). Of 72 cells which satisfied the criteria for antidromic invasion, 55 (76%) and 17 (24%) responded to ipsi- and contralateral olfactory bulb (IOB; COB) stimulation, respectively. The absolute refractory period (3.25±0.3 ms; mean±SE) and the conduction velocity (CV; 1.94±0.2 m/s; mean±SE) of IOB and COB driven neurons were negatively correlated (r=-0.52; p < 0.001). In paired-shock tests (8–1950 ms interval), and early supernormal period (SPN) of increased CV and excitability was found following the relative refractory period in 82% of tested cells (N=50); this period was followed by a late subnormal phase (SBN) of decreased CV and increased threshold in 58% of neurons (N=50). Significant correlations were found to exist between: CV and absolute magnitude of latency variation (r=-0.55; p < 0.001; n=43), CV and duration of SPN and SBN periods (r=-0.60; p < 0.002; n=24 and r=0.58; p < 0.02; n=19, respectively) and between duration of SPN and SBN phases (r=0.79; p < 0.001; n=30). Maximum latency variation during the SPN and SBN periods was attained in a gradual, additive manner. 90% of long-latency units (15–30 ms; n=22) showed progressive increases in initial antidromic latency (16–22% of control) when stimulated with pulse frequencies of 2.5, 10,15, 20 or 40 Hz; initial antidromic latency and magnitude of latency increase were positively correlated (r=0.92). Supported by grants from CONICET, CONICOR and FPPM, Buenos Aires and Cordoba, Argentina     

Effects of UV photofunctionalization on the nanotopography enhanced initial bioactivity of titanium

This study addresses the control of the biological capabilities of titanium through specific nanosurface features and its potential modulation by UV photofunctionalization. Rat bone marrow derived osteoblasts were cultured on titanium disks with micropits alone, micropits with 100 nm nodules, micropits with 300 nm nodules, or micropits with 500 nm nodules, with or without UV treatment. After a 24 h incubation protein adsorption, as well as the attachment, retention, and spread of osteoblasts were examined in correlation with the topographical parameters of the titanium substrates. Each of the biological events was governed by a different set of multiple surface topographical factors with a distinctive pattern of regulation. For instance, without UV treatment the protein adsorption and cell attachment capability of titanium substrates increased linearly with increasing average roughness (Ra) and surface area of titanium disks, but increased polynomially with increasing nanonodule diameter. The cell retention capability increased polynomially with increasing nanonodular diameter and Ra, but increased linearly with increasing surface area. Consequently, the micropits with 300 nm nodules created the most favorable environment for this initial osteoblast behavior and response. UV treatment of the nanonodular titanium surfaces resulted in considerable enhancement of all biological events. However, the pattern of UV-mediated enhancement was disproportionate; exponential and overriding effects were observed depending upon the biological event and topographical parameter. As an example of overriding enhancement, the cell retention capability, which fluctuated with changes in various topographical parameters, became invariably high after UV treatment. The present data provide a basis for understanding how to optimize nanostructures to create titanium surfaces with increased biological capabilities and uncover a novel advantage of UV photofunctionalization of titanium substrates that synergistically increases its nanotopography enhanced biological capabilities whereby most of the initial biological events of osteoblasts were overwhelmingly enhanced beyond a simple proportional increase.